FastQCFastQC Report
Wed 25 May 2016
SRR1780286_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780286_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138006
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAAAG150.002220746870.016
GATCGCT150.002220746870.036
CGTGATA150.002220746870.029
GTTCGGA150.002220746870.056
GAGTTGA150.002220746870.07
TATAGAG150.002220746870.07
GTGTCGA308.341245E-658.33333236
TAGAGAG200.00693914652.59
TTCGTGA200.00693914652.527
ACGATAG200.00693914652.523
CTGGATA200.00693914652.544
GACAGAA305.8613386E-446.66666414
TTGCTGG305.8613386E-446.66666430
TGTATAA305.8613386E-446.66666411
CAAGGGA305.8613386E-446.66666447
GTCCCCT305.8613386E-446.66666418
CGTTGCT404.5737175E-543.7528
CCAGAAA350.001251933340.00000413
TCTGTAC350.001251933340.0000043
TTCGGTC350.001251933340.00000447
Produced by FastQC (version 0.11.5)