FastQCFastQC Report
Wed 25 May 2016
SRR1780286_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780286_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138006
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT109177.910525629320464No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC32852.3803312899439155No Hit
CACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCTG27782.01295595843659TruSeq Adapter, Index 12 (96% over 26bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG26731.9368723098995695No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC6640.48113850122458446No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT2960.21448342825674246TruSeq Adapter, Index 12 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT2330.16883323913453038No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1710.12390765618886135No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1640.11883541295306002No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC1630.11811080677651697No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAA150.002220746870.016
AAAACCG150.002220746870.069
TTGCTGC252.3836464E-455.99999649
TCTTTAT850.053.529411
CTTTATA850.053.529412
AGCGTTC200.00693914652.537
AAGTAGT200.00693914652.537
AAAAGCA200.00693914652.515
TCTAGTT200.00693914652.543
CTACGTA200.00693914652.522
CACTACC200.00693914652.556
GAACAAT200.00693914652.56
CCCATAG200.00693914652.51
AAAGCGT200.00693914652.535
AATGTAG200.00693914652.523
CAAGGTT352.0778481E-550.00000433
CTGCAAG506.148366E-848.99999630
CTAGACT305.8613386E-446.66666417
AAACTCG305.8613386E-446.66666436
CACATAT305.8613386E-446.6666647
Produced by FastQC (version 0.11.5)