##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780286_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 138006 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.72892482935524 34.0 34.0 34.0 34.0 34.0 2 33.73351883251453 34.0 34.0 34.0 34.0 34.0 3 33.74359085836848 34.0 34.0 34.0 34.0 34.0 4 33.76440879382056 34.0 34.0 34.0 34.0 34.0 5 33.759264089967104 34.0 34.0 34.0 34.0 34.0 6 37.50399258003275 38.0 38.0 38.0 37.0 38.0 7 37.508311232844946 38.0 38.0 38.0 37.0 38.0 8 37.41709056127994 38.0 38.0 38.0 37.0 38.0 9 37.4308073562019 38.0 38.0 38.0 37.0 38.0 10-11 37.37529165398606 38.0 38.0 38.0 37.0 38.0 12-13 37.35743373476515 38.0 38.0 38.0 37.0 38.0 14-15 37.288103415793515 38.0 38.0 38.0 36.0 38.0 16-17 37.320486065823225 38.0 38.0 38.0 36.0 38.0 18-19 37.257514166050754 38.0 38.0 38.0 36.0 38.0 20-21 37.29258872802632 38.0 38.0 38.0 36.0 38.0 22-23 37.27868715852934 38.0 38.0 38.0 36.0 38.0 24-25 37.30261003144791 38.0 38.0 38.0 36.0 38.0 26-27 37.20006014231265 38.0 38.0 38.0 36.0 38.0 28-29 37.16392040925757 38.0 38.0 38.0 36.0 38.0 30-31 37.14550816631161 38.0 38.0 38.0 36.0 38.0 32-33 37.091249655812064 38.0 38.0 38.0 36.0 38.0 34-35 37.0738663536368 38.0 38.0 38.0 36.0 38.0 36-37 37.076420590409114 38.0 38.0 38.0 36.0 38.0 38-39 36.50870252018028 38.0 37.0 38.0 34.0 38.0 40-41 36.785473095372666 38.0 37.0 38.0 34.5 38.0 42-43 37.03136457835167 38.0 38.0 38.0 36.0 38.0 44-45 37.10943364781241 38.0 38.0 38.0 36.0 38.0 46-47 37.05149413793603 38.0 38.0 38.0 36.0 38.0 48-49 37.07177948784835 38.0 38.0 38.0 36.0 38.0 50-51 36.91705433097111 38.0 38.0 38.0 35.0 38.0 52-53 36.92035491210527 38.0 38.0 38.0 35.0 38.0 54-55 36.89067866614495 38.0 38.0 38.0 35.5 38.0 56-57 36.936281755865686 38.0 38.0 38.0 35.5 38.0 58-59 36.80397953712158 38.0 37.5 38.0 35.0 38.0 60-61 36.79904134602843 38.0 37.0 38.0 35.0 38.0 62-63 36.79106343202469 38.0 37.0 38.0 35.0 38.0 64-65 36.67605393968378 38.0 37.5 38.0 34.5 38.0 66-67 36.32534817326783 38.0 37.0 38.0 34.0 38.0 68-69 36.199194237931685 38.0 37.0 38.0 34.0 38.0 70-71 35.40120719389012 38.0 36.5 38.0 29.0 38.0 72-73 34.07498949321044 38.0 35.5 38.0 22.0 38.0 74-75 33.667525324985874 38.0 35.0 38.0 19.5 38.0 76 33.027187223743894 38.0 34.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05033398924722832 1101 2 -0.07934315231671007 1101 3 0.030029775967847172 1101 4 0.03189777234603497 1101 5 0.010517622531466486 1101 6 -0.42603628372344815 1101 7 -0.07802751193120372 1101 8 -0.11191439980284201 1101 9 -0.11759470693760932 1101 10-11 -0.13029759523621465 1101 12-13 -0.014190810511692575 1101 14-15 0.1648416122505978 1101 16-17 -0.04686918306637722 1101 18-19 -0.02346377619601725 1101 20-21 -0.09752272457618005 1101 22-23 0.07272227492264705 1101 24-25 0.06669401988523305 1101 26-27 -0.042017932428727534 1101 28-29 -0.36824196108086227 1101 30-31 -0.11548837635465503 1101 32-33 -0.12689517861310406 1101 34-35 -0.16835020261120093 1101 36-37 -0.18804184867418883 1101 38-39 -0.11027645396781338 1101 40-41 0.02878492382328801 1101 42-43 -0.0388339731539773 1101 44-45 -0.028426353990809616 1101 46-47 -0.12012482220310261 1101 48-49 -0.015623095831273304 1101 50-51 -0.1703340022636226 1101 52-53 -0.18287872888149082 1101 54-55 -0.14508743097110255 1101 56-57 -0.09430304167868542 1101 58-59 0.04644231548958544 1101 60-61 -0.3108502745899244 1101 62-63 -0.2650510067916443 1101 64-65 -0.1664928860584851 1101 66-67 -0.20748869182622087 1101 68-69 -0.5672844074435446 1101 70-71 -0.1962177627907593 1101 72-73 0.06466732087853444 1101 74-75 0.48479818349884596 1101 76 -0.7342828518621971 1102 1 0.12436055818905345 1102 2 0.048218307448138376 1102 3 0.02604443469437001 1102 4 -0.029821574119068828 1102 5 -0.010416871284377294 1102 6 0.14069146097039464 1102 7 0.13595935629295042 1102 8 -0.016999145565556262 1102 9 0.21761677573149996 1102 10-11 0.014610024247673437 1102 12-13 0.1234256194776151 1102 14-15 0.10347346444177674 1102 16-17 0.025561722415375243 1102 18-19 0.24834107950714213 1102 20-21 0.14085718075287446 1102 22-23 0.12797498526010287 1102 24-25 0.11488458333003138 1102 26-27 0.13824623003882408 1102 28-29 0.02293226399166315 1102 30-31 0.1876571420451043 1102 32-33 0.27973178733589066 1102 34-35 0.32162536617792625 1102 36-37 0.1840531276580748 1102 38-39 0.1441898767697012 1102 40-41 0.20939265019372755 1102 42-43 0.27720664373409676 1102 44-45 0.2240549408633683 1102 46-47 0.17728699639516066 1102 48-49 0.18771329140341209 1102 50-51 0.07511568471148422 1102 52-53 0.1165715688738942 1102 54-55 0.10737095962287668 1102 56-57 0.15584965338926082 1102 58-59 0.4119561343932361 1102 60-61 0.16403138673241102 1102 62-63 0.09981385807322596 1102 64-65 0.32319256210153924 1102 66-67 0.4824716074561124 1102 68-69 0.5210802283787146 1102 70-71 0.5258924830483025 1102 72-73 0.2557499289102978 1102 74-75 0.09903699858512027 1102 76 0.1447826543219577 1103 1 -0.12898617697586445 1103 2 0.025129929814305285 1103 3 0.002434874721167546 1103 4 -0.051353850196804274 1103 5 0.007295882792007546 1103 6 0.08157449276252038 1103 7 0.07944085431989834 1103 8 0.23038377454464154 1103 9 0.04550003088748156 1103 10-11 6.49783014708305E-4 1103 12-13 -0.02559530227057394 1103 14-15 -0.11156264604888122 1103 16-17 -0.11338176652811427 1103 18-19 0.2238864595979777 1103 20-21 0.05025707651641653 1103 22-23 0.15149147695841947 1103 24-25 -0.017246307891767287 1103 26-27 0.10102264281523787 1103 28-29 -0.038935802421853793 1103 30-31 0.026466612672756185 1103 32-33 0.039094455789474125 1103 34-35 -0.1588600779438778 1103 36-37 0.09201604925735296 1103 38-39 -0.1387935198500685 1103 40-41 0.13646807272369443 1103 42-43 0.13384427309899394 1103 44-45 0.3382683278040304 1103 46-47 -0.026997866980615015 1103 48-49 -0.08920949915574283 1103 50-51 -0.005243186556477042 1103 52-53 -0.05165119934888196 1103 54-55 -0.009552575482473458 1103 56-57 -0.24676370376954537 1103 58-59 0.09734813796706021 1103 60-61 0.09089833632663158 1103 62-63 0.12062392433783486 1103 64-65 0.1346697417605398 1103 66-67 0.04941003348545081 1103 68-69 -0.20584256218044317 1103 70-71 -0.03271447101320035 1103 72-73 -0.2930625199021577 1103 74-75 -0.42930042975230975 1103 76 -0.11904729132616865 1104 1 -0.011260049013323226 1104 2 -0.07083828265403014 1104 3 0.05197733810716443 1104 4 -0.006287047635481713 1104 5 -0.003250723903754249 1104 6 -0.02002153084178815 1104 7 -0.07562676065509777 1104 8 -0.5430045074958514 1104 9 -0.2540664356299942 1104 10-11 -0.14291479279457064 1104 12-13 -0.03196167129841143 1104 14-15 -0.18408903102956486 1104 16-17 -0.2213515447391785 1104 18-19 -0.17003077760466567 1104 20-21 -0.035640899096407 1104 22-23 -0.26157287359070125 1104 24-25 0.0014761346668947795 1104 26-27 -0.18364711102341857 1104 28-29 -0.10484470869148055 1104 30-31 -0.27900831697960626 1104 32-33 -0.16567071972881564 1104 34-35 -0.2167704022446486 1104 36-37 -0.2875177730012979 1104 38-39 0.02744109044986942 1104 40-41 0.0498426175873945 1104 42-43 -0.1572546271883013 1104 44-45 -0.12595592791426213 1104 46-47 -0.3905160613542833 1104 48-49 -0.1014215538976515 1104 50-51 -0.4514983287658012 1104 52-53 -0.3276281268003345 1104 54-55 -0.41012052650130926 1104 56-57 -0.6252893945058204 1104 58-59 -0.23622153389167266 1104 60-61 -0.4109812935081507 1104 62-63 -0.3106274142983452 1104 64-65 -0.2693768169289612 1104 66-67 -0.25341347333513653 1104 68-69 -0.2649715070027767 1104 70-71 -0.31829634384830285 1104 72-73 0.15067783268538193 1104 74-75 0.10587708263699369 1104 76 -0.06038363797570412 1105 1 0.07235882383437797 1105 2 0.09578298442458077 1105 3 0.10063613869783694 1105 4 0.018407938816324076 1105 5 0.010698896890211529 1105 6 0.09364709039661534 1105 7 0.12376106174171042 1105 8 0.1418243838462132 1105 9 0.21338205973207636 1105 10-11 0.2882044743127068 1105 12-13 0.18239367844567766 1105 14-15 0.09764169684530088 1105 16-17 0.10319577357883247 1105 18-19 0.26285907343689985 1105 20-21 0.1922518909711215 1105 22-23 -0.1280013118926604 1105 24-25 0.10356069259437817 1105 26-27 0.1585092738312781 1105 28-29 0.22244808997808008 1105 30-31 0.09294692380547076 1105 32-33 0.1259523394387969 1105 34-35 0.1150420448887246 1105 36-37 0.07658094959765549 1105 38-39 0.15828150850838796 1105 40-41 0.16656854266373955 1105 42-43 0.16516010521696245 1105 44-45 -0.13130710567514825 1105 46-47 0.036768135288063775 1105 48-49 0.1352273830351578 1105 50-51 -0.018207049787719143 1105 52-53 0.04772491473312357 1105 54-55 0.10995803868055276 1105 56-57 0.18930824567138416 1105 58-59 -0.05603056888758573 1105 60-61 -0.03375731105628432 1105 62-63 0.08922706807467051 1105 64-65 -0.13011716473759805 1105 66-67 -0.43013136985273803 1105 68-69 0.08811303658799119 1105 70-71 -0.14748517268230188 1105 72-73 -0.10678801009823502 1105 74-75 0.02346249359884922 1105 76 -0.35174863632698106 1106 1 0.03542243974273873 1106 2 -0.04136964115620145 1106 3 0.0782376114777108 1106 4 0.01925537167918634 1106 5 -0.17680292947371612 1106 6 -0.09265664123016393 1106 7 -0.14392544654267425 1106 8 -0.07546909092074827 1106 9 0.17003148552326053 1106 10-11 0.02465285396263539 1106 12-13 0.005791592007525992 1106 14-15 0.04249281165784424 1106 16-17 0.02956408543413147 1106 18-19 0.13787733707619054 1106 20-21 0.10883089216920894 1106 22-23 -0.05307655808651646 1106 24-25 0.03492593615826678 1106 26-27 -0.058689230011388815 1106 28-29 -0.006081175677515205 1106 30-31 -0.029770121283796414 1106 32-33 -0.018045498146314287 1106 34-35 0.09693182017290525 1106 36-37 -0.09137147727473405 1106 38-39 0.22405252630447592 1106 40-41 -0.048759764516056237 1106 42-43 0.08295911751935847 1106 44-45 0.01887845289999035 1106 46-47 -0.17502433132600714 1106 48-49 0.03779452017316487 1106 50-51 0.16228153909045062 1106 52-53 0.02298864168440673 1106 54-55 0.05554700452023553 1106 56-57 -0.031345738724162686 1106 58-59 -0.02649461143455767 1106 60-61 -0.14751786743979522 1106 62-63 0.3742932555034386 1106 64-65 0.2891429387076556 1106 66-67 0.3221260159629864 1106 68-69 0.42389369758562623 1106 70-71 0.40251336622656453 1106 72-73 0.3420591889900422 1106 74-75 0.20905029056562796 1106 76 0.3871397655315292 1107 1 0.1355137599082923 1107 2 0.011014257036542574 1107 3 0.007781157940527805 1107 4 0.04365405784712806 1107 5 0.10069990587012967 1107 6 0.1012072328069209 1107 7 0.04528404486291748 1107 8 0.26224613745331027 1107 9 0.15927190795267165 1107 10-11 -0.08624959547987032 1107 12-13 0.11268037288331811 1107 14-15 0.17781235859199285 1107 16-17 0.052876503503739514 1107 18-19 -0.1452372697315809 1107 20-21 0.01432188534965917 1107 22-23 0.06211572977820623 1107 24-25 -0.043966585898495225 1107 26-27 0.1669378596738511 1107 28-29 0.2325811311877004 1107 30-31 0.060014539874195805 1107 32-33 0.16550435235373584 1107 34-35 -0.10409037651894693 1107 36-37 0.17650960030322693 1107 38-39 0.11000542277392356 1107 40-41 0.07326312444156002 1107 42-43 -0.0013684272938050412 1107 44-45 0.10855264611578264 1107 46-47 0.11862523490320598 1107 48-49 0.14517304225938688 1107 50-51 -0.015115247028880674 1107 52-53 0.12988110482493198 1107 54-55 0.13295557388673984 1107 56-57 -0.04663450192508378 1107 58-59 0.1147681107901235 1107 60-61 -0.16883449802026718 1107 62-63 0.17564980183369272 1107 64-65 0.4026818897040769 1107 66-67 0.41285333689445025 1107 68-69 0.5622327016066606 1107 70-71 0.3251894889490785 1107 72-73 0.7681561356561204 1107 74-75 1.2423895155225395 1107 76 1.176654148457601 1108 1 -0.11246467674938998 1108 2 -0.09949787234351248 1108 3 0.05629867815616052 1108 4 -0.05068556479438513 1108 5 -0.025984730248467258 1108 6 -0.2680650043898396 1108 7 -0.005928893142467473 1108 8 0.10052107006787026 1108 9 -0.07792485754598033 1108 10-11 -0.13341940680062692 1108 12-13 -0.11777784814094616 1108 14-15 -0.008171468901274181 1108 16-17 -0.05494020808654909 1108 18-19 0.043441975551431256 1108 20-21 -0.0436295971301206 1108 22-23 -0.23033710041047328 1108 24-25 -0.09787494169363953 1108 26-27 -0.06082494356065382 1108 28-29 0.046597303694440484 1108 30-31 -0.09227656524709715 1108 32-33 -0.05956303309100264 1108 34-35 0.11693388224115608 1108 36-37 0.06599747092316477 1108 38-39 -0.12719134272472843 1108 40-41 -0.054769220571913024 1108 42-43 0.0848849419784301 1108 44-45 0.010169896789648192 1108 46-47 -0.14417799959813493 1108 48-49 -0.01250889844141767 1108 50-51 0.049574779925301016 1108 52-53 -0.2932994881669799 1108 54-55 -0.2686616767871115 1108 56-57 -0.05337304639947149 1108 58-59 -0.3434529134686386 1108 60-61 -0.16074824465099624 1108 62-63 -0.13701866177816413 1108 64-65 0.018584854663650674 1108 66-67 -0.1787908679573036 1108 68-69 0.044712485973235516 1108 70-71 0.18637547277657518 1108 72-73 0.1792073485941259 1108 74-75 -0.05814956803541094 1108 76 0.7211285419885982 1109 1 0.02514117531844562 1109 2 0.0057335883966587176 1109 3 -3.124398913882942E-4 1109 4 -0.0782640220165618 1109 5 0.09401644486555938 1109 6 -0.3962405664248436 1109 7 -0.16670652484704362 1109 8 -0.24806181586528453 1109 9 0.05940924940408365 1109 10-11 -0.051447859248902716 1109 12-13 0.038846489939359685 1109 14-15 -0.051129230896613365 1109 16-17 -0.046376564645527196 1109 18-19 0.019601849814236516 1109 20-21 0.1608402428340341 1109 22-23 -0.06875157781522745 1109 24-25 -0.10091554922759371 1109 26-27 0.017173249701535553 1109 28-29 -0.19052601717778828 1109 30-31 -0.13777551131671117 1109 32-33 -0.05368746297708782 1109 34-35 -0.11755277654245333 1109 36-37 -0.09824674714861459 1109 38-39 -0.07094010334665768 1109 40-41 -0.009920259446154489 1109 42-43 -0.19131092015131657 1109 44-45 -0.18451711893484912 1109 46-47 -0.035817797772303095 1109 48-49 -0.18055020369931185 1109 50-51 -0.32017851957454724 1109 52-53 -0.15948705105394367 1109 54-55 -0.18820308853972278 1109 56-57 0.07101277291205577 1109 58-59 -0.12817936895753235 1109 60-61 -0.05128270119747924 1109 62-63 -0.1083752574728436 1109 64-65 -0.08817425430614634 1109 66-67 0.27262183943195595 1109 68-69 0.19464234635500333 1109 70-71 0.6332015632456631 1109 72-73 0.28760794496449904 1109 74-75 0.6302322265105857 1109 76 0.84649117483157 1110 1 -0.007556372365478126 1110 2 0.02480715787895349 1110 3 0.09778020316038294 1110 4 0.08249892076273113 1110 5 0.09129164922523358 1110 6 -0.009319585498417382 1110 7 0.1112226304663082 1110 8 0.2015294647886705 1110 9 0.23379617038500555 1110 10-11 0.1460998246748062 1110 12-13 0.2541053455251898 1110 14-15 0.12053289444398274 1110 16-17 0.15389591491850751 1110 18-19 0.23758550104039244 1110 20-21 0.08727076054520921 1110 22-23 0.10563534316569445 1110 24-25 0.10889371507758483 1110 26-27 0.0498707973854593 1110 28-29 0.25906526347615966 1110 30-31 0.07475854862880027 1110 32-33 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0.27412771657088086 2115 54-55 0.23588256982463207 2115 56-57 0.4285942289507432 2115 58-59 0.2551993776364796 2115 60-61 0.3496613829257882 2115 62-63 0.3110053312365224 2115 64-65 0.02500070827588985 2115 66-67 0.04705097555660842 2115 68-69 0.03230976777727079 2115 70-71 -0.27858029959349295 2115 72-73 -0.15704032613347607 2115 74-75 -0.0861933021381418 2115 76 -0.18300569741006 2116 1 0.0788650626935592 2116 2 0.15384893807876665 2116 3 0.0781465367964671 2116 4 -0.02591767021516489 2116 5 -0.13053699140449737 2116 6 0.12259836787730194 2116 7 0.07597437130796436 2116 8 0.19133418776777944 2116 9 0.013337496452223263 2116 10-11 0.07069110532874845 2116 12-13 0.00916135521335093 2116 14-15 0.13459208306039017 2116 16-17 -0.14482116046750804 2116 18-19 -0.07200426583820274 2116 20-21 -0.09679296939727067 2116 22-23 0.06919120147632185 2116 24-25 0.015823021768476053 2116 26-27 0.05784082463341633 2116 28-29 0.07210643816583939 2116 30-31 -0.15524826336029918 2116 32-33 -0.08753547993137545 2116 34-35 0.06441815897072445 2116 36-37 -0.014595520990582145 2116 38-39 -0.1910203334405054 2116 40-41 -0.1627169594158815 2116 42-43 0.014744181271630907 2116 44-45 -0.18979641048798612 2116 46-47 0.03599570510386485 2116 48-49 -0.2086080299179045 2116 50-51 0.18416223125803555 2116 52-53 -0.19697948217716288 2116 54-55 -0.17851492626299859 2116 56-57 -0.025494190454580234 2116 58-59 0.09825493319203815 2116 60-61 -0.05004518984416251 2116 62-63 0.4099489932083529 2116 64-65 -0.21404467513569614 2116 66-67 0.042982117966623434 2116 68-69 -0.21274109294260768 2116 70-71 -0.380751661095843 2116 72-73 -0.5373807017203305 2116 74-75 -0.3505125509644387 2116 76 -0.11144857690927523 2117 1 -0.09968005701178129 2117 2 -0.09978826265788143 2117 3 -0.2717306884951185 2117 4 0.058367167108343665 2117 5 0.05921132044326072 2117 6 0.09977761895158466 2117 7 0.13354036271373815 2117 8 -0.4639604715324026 2117 9 -0.005677236512788397 2117 10-11 0.08049773516300718 2117 12-13 -0.00946970678787551 2117 14-15 0.2301645201383522 2117 16-17 0.11100296476269023 2117 18-19 0.13546964453625776 2117 20-21 0.1327266745560678 2117 22-23 0.14170501363101806 2117 24-25 -0.12967268111673746 2117 26-27 0.23587949866657 2117 28-29 -0.509492702411201 2117 30-31 -0.07520682689621339 2117 32-33 -0.17323222640160196 2117 34-35 0.07445499138324152 2117 36-37 0.12021110884367658 2117 38-39 0.14269667085660132 2117 40-41 0.11832202155404303 2117 42-43 0.12336910453743855 2117 44-45 0.16828370000925474 2117 46-47 0.1789668530473918 2117 48-49 0.09174494736877392 2117 50-51 0.24446763334520227 2117 52-53 0.026785097319461215 2117 54-55 0.08130705040979791 2117 56-57 0.053565048342221644 2117 58-59 0.1648678859237691 2117 60-61 0.04233510481513747 2117 62-63 0.09820866171664022 2117 64-65 0.17284912474153202 2117 66-67 0.2079606323926555 2117 68-69 0.20991851417832663 2117 70-71 0.4462971418507493 2117 72-73 0.10845263161299812 2117 74-75 0.23585945640205352 2117 76 -0.15520241361891607 2118 1 -0.03274542419782733 2118 2 -0.1280799008725424 2118 3 -0.24120427468917427 2118 4 -0.0035081820753433135 2118 5 -0.08384535594881726 2118 6 0.15777314940039844 2118 7 0.04317162474116998 2118 8 -0.023740718599263744 2118 9 -0.09686849605714087 2118 10-11 0.06900571207032868 2118 12-13 0.21559081838439198 2118 14-15 0.09191105934004185 2118 16-17 -0.027452246609826148 2118 18-19 0.010267893962044639 2118 20-21 0.10497045132682103 2118 22-23 0.05655075203812032 2118 24-25 0.18812327145636232 2118 26-27 0.12413295946488034 2118 28-29 0.01614513978881149 2118 30-31 0.19601578158351884 2118 32-33 0.11483655502492951 2118 34-35 0.11423089305311862 2118 36-37 0.07360672620043829 2118 38-39 -0.04666952195486829 2118 40-41 -0.024998482512010867 2118 42-43 -0.07274021323523527 2118 44-45 -0.12589191610596373 2118 46-47 0.18062741546591354 2118 48-49 0.22129209492604218 2118 50-51 0.25674337982232487 2118 52-53 0.09794873255442837 2118 54-55 0.22847633466084005 2118 56-57 0.03422803176763978 2118 58-59 0.04705331022074688 2118 60-61 0.156743199668945 2118 62-63 0.06500517298363917 2118 64-65 0.11148591163084376 2118 66-67 0.27207075716762574 2118 68-69 0.24932195324965534 2118 70-71 0.09738504302401196 2118 72-73 0.847216676556819 2118 74-75 0.8263601082116026 2118 76 0.5505676528035863 2119 1 -0.10561769592713688 2119 2 0.08172250129715763 2119 3 0.03772508085776849 2119 4 -0.028982804314786392 2119 5 -0.08494312167270124 2119 6 0.10985890427577516 2119 7 -0.03044325321310737 2119 8 -0.09228650188739351 2119 9 -0.03177744607651789 2119 10-11 -0.04367671076320079 2119 12-13 -0.054152821031856035 2119 14-15 -0.568233587437696 2119 16-17 -0.08347058575486699 2119 18-19 -0.055146028290309346 2119 20-21 -0.08390982763481958 2119 22-23 0.08355901756827677 2119 24-25 -0.043037131488233626 2119 26-27 0.10036956026559807 2119 28-29 -0.017740305129180456 2119 30-31 -0.30711607945224983 2119 32-33 -0.30410636115743017 2119 34-35 -0.3789726456269804 2119 36-37 0.13540447900942354 2119 38-39 -0.18192071658227604 2119 40-41 -0.16678784064193053 2119 42-43 -0.04971942026093501 2119 44-45 0.007761060776381612 2119 46-47 0.05745164380118695 2119 48-49 0.11792453713189843 2119 50-51 0.03478483662201626 2119 52-53 0.09607607337839141 2119 54-55 0.1323088285262699 2119 56-57 0.14198665245729103 2119 58-59 -0.13072016259340558 2119 60-61 0.14779963774204674 2119 62-63 0.2392593380359429 2119 64-65 -0.13885310425447273 2119 66-67 0.27047253942256333 2119 68-69 0.12772825571639856 2119 70-71 0.1915184538466903 2119 72-73 -0.15878874769734352 2119 74-75 0.2109817019091338 2119 76 -1.1285941707790066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 2.0 22 6.0 23 16.0 24 32.0 25 64.0 26 149.0 27 212.0 28 447.0 29 755.0 30 1186.0 31 1836.0 32 2861.0 33 4820.0 34 8117.0 35 13485.0 36 20317.0 37 83700.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.41802530324769 16.03843311160384 12.506702607133025 36.03683897801545 2 18.60208976421315 18.253554193295944 37.17084764430532 25.973508398185587 3 18.06515658739475 17.710099560888658 33.23478689332348 30.989956958393112 4 14.917467356491745 24.06634494152428 31.892091648189208 29.124096053794762 5 15.387736765068185 29.15308030085648 39.723635204266486 15.735547729808848 6 28.48426879990725 37.783139863484195 16.395663956639567 17.336927379968987 7 26.648116748547164 24.071417184760083 21.99614509514079 27.284320971551963 8 23.05769314377636 37.55343970552005 15.615987710679246 23.772879440024347 9 21.66065243540136 15.821775864817472 16.57536628842224 45.94220541135893 10-11 25.866628987145486 26.827456777241572 23.31021839630161 23.995695839311335 12-13 24.804356332333377 18.344854571540367 25.90032317435474 30.95046592177152 14-15 21.883831137776617 17.595611784994855 20.1397040708375 40.38085300639103 16-17 28.273046099444954 24.67211570511427 18.365143544483573 28.689694650957204 18-19 30.796124806167846 22.804443285074562 19.08648899323218 27.31294291552541 20-21 24.952900598524703 20.507441705433095 19.009680738518615 35.52997695752359 22-23 23.152616552903496 24.703998376882165 20.003478109647407 32.13990696056693 24-25 28.124139530165355 26.164442125704678 18.672376563337824 27.03904178079214 26-27 29.119386113647234 26.699201483993452 21.386388997579818 22.795023404779503 28-29 24.90725040940249 23.145370491138067 24.188803385360057 27.758575714099386 30-31 22.470037534599946 26.258278625567005 22.36678115444256 28.90490268539049 32-33 21.725866991290232 21.358491659782906 30.44722693216237 26.468414416764492 34-35 27.76437256351173 23.411663261017637 25.490558381519644 23.333405793950988 36-37 24.798921786009302 31.327623436662172 20.8903960697361 22.983058707592424 38-39 21.21429503065084 24.89529440748953 27.094474153297682 26.79593640856195 40-41 22.18418039795371 18.793385794820516 28.347680535628882 30.674753271596888 42-43 27.402794081416747 18.39992464095764 27.43866208715563 26.75861919046998 44-45 21.665000072460618 24.285538309928555 30.247235627436485 23.802225990174342 46-47 25.2162949436981 20.59584365897135 27.445545845832793 26.742315551497757 48-49 25.41302552062954 19.01294146631306 23.437386780284918 32.136646232772485 50-51 22.86060026375665 19.441546019738272 27.66944915438459 30.028404562120485 52-53 18.598466733330437 19.674144602408592 36.037563584191986 25.689825080068985 54-55 19.315102241931513 20.51577467646334 31.207338811356028 28.96178427024912 56-57 23.613466081184875 21.714273292465545 24.81160239409881 29.86065823225077 58-59 20.392591626451022 22.138167905743227 31.13197976899555 26.337260698810194 60-61 23.96634928916134 26.1807457646769 25.48113850122458 24.371766444937176 62-63 19.44842977841543 35.040143182180486 20.711418344129964 24.800008695274116 64-65 18.406083793458254 37.14874715592076 19.864716026839414 24.580453023781576 66-67 18.67128965407301 36.61797313160297 19.779574801095603 24.93116241322841 68-69 18.15464545019782 37.03172325840905 20.031737750532585 24.78189354086054 70-71 18.151747025491645 35.67091285886121 20.67446342912627 25.502876686520874 72-73 18.438691071402694 32.60945176296683 21.46609567699955 27.485761488630928 74-75 18.42927119110763 31.093937944727042 22.957697491413416 27.519093372751907 76 18.658609045983507 31.66166688404852 22.810602437575177 26.869121632392794 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 2.5 17 4.0 18 5.5 19 8.5 20 8.5 21 7.0 22 16.0 23 45.0 24 66.5 25 68.0 26 130.5 27 220.5 28 363.0 29 478.0 30 530.5 31 693.5 32 1007.0 33 1210.0 34 1348.5 35 1755.5 36 2250.0 37 2476.0 38 5197.5 39 9577.5 40 10733.0 41 9034.0 42 7838.0 43 7174.0 44 6476.5 45 6241.0 46 6039.0 47 6016.5 48 5651.0 49 5031.5 50 4755.0 51 4352.0 52 3618.0 53 3141.0 54 2995.0 55 3020.5 56 2827.0 57 2597.5 58 2587.0 59 2527.5 60 2553.5 61 2750.5 62 2862.0 63 2928.0 64 3064.5 65 3210.5 66 3370.5 67 3455.0 68 3374.0 69 3235.5 70 3032.5 71 2887.0 72 2698.0 73 2199.5 74 1625.5 75 1361.0 76 1143.5 77 680.0 78 304.0 79 174.0 80 114.0 81 35.5 82 12.0 83 7.0 84 4.0 85 0.5 86 0.5 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 138006.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.18955697578367 #Duplication Level Percentage of deduplicated Percentage of total 1 57.86456079619212 23.25551062997261 2 21.709577383528053 17.449965943509703 3 8.955358430693783 10.79735663666797 4 3.8024664647338815 6.11277770531716 5 2.0031011106303187 4.025187310696636 6 1.3702581854896871 3.3042041650363028 7 0.9718015289196595 2.7339391040969234 8 0.7608538872061157 2.446270452009333 9 0.503028991778451 1.8194861092995958 >10 2.0229337948939854 12.044403866498559 >50 0.01622674167027261 0.45432807269249165 >100 0.010817827780181739 0.8347463153775923 >500 0.0018029712966969567 0.48113850122458446 >1k 0.0054089138900908695 6.330159558280076 >5k 0.0 0.0 >10k+ 0.0018029712966969567 7.910525629320464 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT 10917 7.910525629320464 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 3285 2.3803312899439155 No Hit CACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCTG 2778 2.01295595843659 TruSeq Adapter, Index 12 (96% over 26bp) TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 2673 1.9368723098995695 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 664 0.48113850122458446 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT 296 0.21448342825674246 TruSeq Adapter, Index 12 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT 233 0.16883323913453038 No Hit CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 171 0.12390765618886135 No Hit CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 164 0.11883541295306002 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 163 0.11811080677651697 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0036230308827152444 0.0 2 0.0 0.0 0.0 0.008695274118516587 0.0 3 0.0 0.0 0.0 0.008695274118516587 0.0 4 0.0 0.0 0.0 0.053620857064185616 0.0 5 0.0 0.0 0.0 0.0652145558888744 0.0 6 0.0 0.0 0.0 0.0652145558888744 0.0 7 0.0 0.0 0.0 0.06738837441850354 0.0 8 0.0 0.0 0.0 0.06738837441850354 0.0 9 0.0 0.0 0.0 0.07463443618393403 0.0 10 0.0 0.0 0.0 0.07898207324319233 0.0 11 0.0 0.0 0.0 0.08115589177282147 0.0 12 0.0 0.0 0.0 0.08332971030245062 0.0 13 0.0 0.0 0.0 0.08477892265553671 0.0 14 0.0 0.0 0.0 0.08840195353825196 0.0 15 0.0 0.0 0.0 0.08840195353825196 0.0 16 0.0 0.0 0.0 0.08985116589133806 0.0 17 0.0 0.0 0.0 0.09419880295059635 0.0 18 0.0 0.0 0.0 0.09999565236294074 0.0 19 0.0 0.0 0.0 0.10289407706911294 0.0 20 0.0 0.0 0.0 0.11014013883454342 0.0 21 0.0 0.0 0.0 0.11521238207034477 0.0 22 0.0 0.0 0.0 0.1231830500123183 0.0 23 0.0 0.0 0.0 0.13115371795429184 0.0 24 0.0 0.0 0.0 0.13332753648392098 0.0 25 0.0 0.0 0.0 0.13912438589626538 0.0 26 0.0 0.0 0.0 0.14854426619132502 0.0 27 0.0 0.0 0.0 0.2478153123777227 0.0 28 0.0 0.0 0.0 0.33984029679868993 0.0 29 0.0 0.0 0.0 0.4499804356332333 0.0 30 0.0 0.0 0.0 0.578235728881353 0.0 31 0.0 0.0 0.0 0.7130124777183601 0.0 32 0.0 0.0 0.0 0.8717012303812878 0.0 33 0.0 0.0 0.0 1.1245887859948118 0.0 34 0.0 0.0 0.0 1.4064605886700579 0.0 35 0.0 0.0 0.0 1.8180368969465095 0.0 36 0.0 0.0 0.0 2.401344869063664 0.0 37 0.0 0.0 0.0 3.2418880338536007 0.0 38 0.0 0.0 0.0 4.011419793342318 0.0 39 0.0 0.0 0.0 4.754141124298943 0.0 40 0.0 0.0 0.0 5.530918945553092 0.0 41 0.0 0.0 0.0 6.322913496514644 0.0 42 0.0 0.0 0.0 7.206208425720621 0.0 43 0.0 0.0 0.0 8.119936814341406 0.0 44 0.0 0.0 0.0 8.997434894135038 0.0 45 0.0 0.0 0.0 9.858267031868179 0.0 46 0.0 0.0 0.0 10.791559787255627 0.0 47 0.0 0.0 0.0 11.571236033215946 0.0 48 0.0 0.0 0.0 12.37047664594293 0.0 49 0.0 0.0 0.0 13.104502702781039 0.0 50 0.0 0.0 0.0 13.781284871672247 0.0 51 0.0 0.0 0.0 14.480529832036288 0.0 52 0.0 0.0 0.0 15.1725287306349 0.0 53 0.0 0.0 0.0 15.906554787473008 0.0 54 0.0 0.0 0.0 16.557251134008666 0.0 55 0.0 0.0 0.0 17.244177789371477 0.0 56 0.0 0.0 0.0 17.92023535208614 0.0 57 0.0 0.0 0.0 18.56151181832674 0.0 58 0.0 0.0 0.0 19.12525542367723 0.0 59 0.0 0.0 0.0 19.67233308696723 0.0 60 0.0 0.0 0.0 20.283900699969568 0.0 61 0.0 0.0 0.0 20.928075590916336 0.0 62 0.0 0.0 0.0 21.491819196266828 0.0 63 0.0 0.0 0.0 22.06208425720621 0.0 64 0.0 0.0 0.0 22.650464472559165 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCACAA 15 0.0022207468 70.0 16 AAAACCG 15 0.0022207468 70.0 69 TTGCTGC 25 2.3836464E-4 55.999996 49 TCTTTAT 85 0.0 53.52941 1 CTTTATA 85 0.0 53.52941 2 AGCGTTC 20 0.006939146 52.5 37 AAGTAGT 20 0.006939146 52.5 37 AAAAGCA 20 0.006939146 52.5 15 TCTAGTT 20 0.006939146 52.5 43 CTACGTA 20 0.006939146 52.5 22 CACTACC 20 0.006939146 52.5 56 GAACAAT 20 0.006939146 52.5 6 CCCATAG 20 0.006939146 52.5 1 AAAGCGT 20 0.006939146 52.5 35 AATGTAG 20 0.006939146 52.5 23 CAAGGTT 35 2.0778481E-5 50.000004 33 CTGCAAG 50 6.148366E-8 48.999996 30 CTAGACT 30 5.8613386E-4 46.666664 17 AAACTCG 30 5.8613386E-4 46.666664 36 CACATAT 30 5.8613386E-4 46.666664 7 >>END_MODULE