FastQCFastQC Report
Wed 25 May 2016
SRR1780285_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780285_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542117
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTA602.955785E-729.31574233
ACAAGTG450.001398346524.4410627
AGTACCA450.001405129124.420775
CTTTGTA500.002571021422.01117740
ACCTTTG500.002587615721.98680742
AAATCGT803.6282836E-621.98680532
CTCTAGC500.002593166721.9786932
AAAATCG856.134951E-620.69346431
ATCGTAG755.444153E-520.54376634
ATACAGG755.5064207E-520.5134476
TAGATCT1950.020.31800839
TTCATAC654.9596344E-420.2880253
GTCCCTA550.004472098320.00646412
CAAGTGA550.004484064419.9972328
ACAGAGC550.00450566919.980638
AGTTAGT708.138117E-418.84931210
CCCAATG951.5963615E-518.5237530
AATGTGT951.602721E-518.51520533
CATGGTT600.00737741518.34603714
TGACGCT7200.018.34603513
Produced by FastQC (version 0.11.5)