FastQCFastQC Report
Wed 25 May 2016
SRR1780285_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780285_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542117
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT223724.126784439521359No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC73031.3471261738702163No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG55761.028560255443013No Hit
CACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCTG43550.8033321220326977Illumina PCR Primer Index 2 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC13050.2407229435712217No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAAGC250.002348509235.2095953
ATGCCGT29500.033.85436244
TCTTCTG5150.033.31717744
GTCTTCT5150.033.31717743
CTTTATA1250.031.6886372
TCAGTTA353.217358E-431.4255441
CCGTCTT5600.030.63990241
CGTCTTC5700.030.48828942
CACTACA454.057899E-529.341332
ACCGTAT300.00574496129.3305058
CGCAACT300.00574496129.33050520
TATGCCG35950.027.78035543
GTTCCGA406.91808E-427.5736451
TCTTTAT1450.027.3834821
TTAATAC905.11136E-1026.896223
TCCGTAT508.338568E-526.39745313
CAGTTAT450.001398005624.44208742
AGTTATA450.001398005624.44208743
GCCGTCT7150.023.99768440
GTAGCGT651.8123508E-523.69002313
Produced by FastQC (version 0.11.5)