FastQCFastQC Report
Wed 25 May 2016
SRR1780284_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780284_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194227
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAT150.002222113870.09
GACCTAG150.002222113870.01
CGGAATA200.006943383752.569
GATACTT200.006943383752.518
TGCATAT200.006943383752.56
AGAGGGT501.7052644E-435.06
GGCTATA400.002414502435.01
ATAAGAG751.483395E-632.6666683
GCGATGT450.004299734331.11111319
CAGCTAC450.004299734331.1111131
TAAGAGA853.9367605E-628.823534
GTATAAG755.564538E-528.01
CGTATCC500.00719585427.99999856
CAGCCGT500.00719585427.99999856
TTCGGTC500.00719585427.99999828
TGAATGG500.00719585427.99999841
CATTAAA906.141003E-627.22222361
AGAGACA906.141003E-627.2222236
TATAAGA808.634314E-526.252
AAGAGAC959.3454655E-625.7894735
Produced by FastQC (version 0.11.5)