##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780284_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 194227 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.621978406709676 34.0 32.0 34.0 21.0 34.0 2 30.717809573334293 34.0 32.0 34.0 21.0 34.0 3 30.494447218975736 34.0 32.0 34.0 21.0 34.0 4 30.40973706024394 34.0 32.0 34.0 11.0 34.0 5 30.41308365984132 34.0 32.0 34.0 11.0 34.0 6 33.26967414417151 37.0 34.0 38.0 21.0 38.0 7 33.74564298475495 38.0 34.0 38.0 21.0 38.0 8 33.620027081713665 38.0 34.0 38.0 21.0 38.0 9 34.01238756712507 38.0 35.0 38.0 21.0 38.0 10-11 33.796611696623025 38.0 34.0 38.0 21.0 38.0 12-13 33.85674751708053 38.0 34.0 38.0 21.0 38.0 14-15 33.817025954167036 38.0 34.0 38.0 21.0 38.0 16-17 33.87884794596014 38.0 34.0 38.0 21.0 38.0 18-19 33.88433894360722 38.0 34.5 38.0 21.0 38.0 20-21 33.87835882755745 38.0 34.0 38.0 21.0 38.0 22-23 33.88724276233479 38.0 34.5 38.0 21.0 38.0 24-25 33.90937923151776 38.0 35.0 38.0 21.0 38.0 26-27 33.89043490348922 38.0 35.0 38.0 21.0 38.0 28-29 33.98248956118356 38.0 35.0 38.0 21.0 38.0 30-31 33.98669597944673 38.0 35.0 38.0 21.0 38.0 32-33 34.037175572912105 38.0 35.0 38.0 21.0 38.0 34-35 34.00658765259207 38.0 35.0 38.0 21.0 38.0 36-37 33.918031478630674 38.0 35.0 38.0 21.0 38.0 38-39 33.90442368980626 38.0 35.0 38.0 21.0 38.0 40-41 33.98074932939293 38.0 35.0 38.0 21.0 38.0 42-43 34.05982947787898 38.0 35.0 38.0 21.0 38.0 44-45 34.11246376662359 38.0 35.5 38.0 21.0 38.0 46-47 34.03884114978865 38.0 35.5 38.0 21.0 38.0 48-49 33.99776807549929 38.0 35.0 38.0 21.0 38.0 50-51 33.95109588265277 38.0 35.0 38.0 21.0 38.0 52-53 33.99786332487244 38.0 35.0 38.0 21.0 38.0 54-55 33.993245017428066 38.0 35.0 38.0 21.0 38.0 56-57 33.934437539579974 38.0 35.0 38.0 21.0 38.0 58-59 33.991419833493794 38.0 35.0 38.0 21.0 38.0 60-61 34.012796881998895 38.0 35.5 38.0 21.0 38.0 62-63 33.87725444969031 38.0 35.0 38.0 20.5 38.0 64-65 33.865232434213574 38.0 35.0 38.0 20.0 38.0 66-67 33.769517111421166 38.0 34.5 38.0 20.0 38.0 68-69 33.70329305400382 38.0 34.0 38.0 20.0 38.0 70-71 33.714460399429534 38.0 34.0 38.0 19.5 38.0 72-73 33.722978782558556 38.0 34.5 38.0 19.0 38.0 74-75 33.74340591163947 38.0 35.0 38.0 19.0 38.0 76 32.11126671369068 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.35558439189291136 1101 2 -0.17581151481246948 1101 3 -0.20898998556065962 1101 4 0.5067366205995256 1101 5 -0.36036140292931407 1101 6 0.1485756960250626 1101 7 -0.3354934332141326 1101 8 -0.24751944362730427 1101 9 -0.5165416176303808 1101 10-11 -0.30822922465738145 1101 12-13 -0.14596154763023605 1101 14-15 -0.7352343853803305 1101 16-17 -0.28040041247521685 1101 18-19 0.044357597021672746 1101 20-21 0.08110545049640194 1101 22-23 -0.033177067826144935 1101 24-25 -0.175421926529431 1101 26-27 -0.44383722210911003 1101 28-29 -0.2907046429162463 1101 30-31 -0.163259770713843 1101 32-33 -0.5008428783152752 1101 34-35 -0.6278059724346292 1101 36-37 -0.3707173565849402 1101 38-39 -0.019932028405371227 1101 40-41 -0.10599594450082606 1101 42-43 -0.28452985703694367 1101 44-45 0.09277543407232969 1101 46-47 0.07134746434749673 1101 48-49 -0.016541464370376957 1101 50-51 -0.26897085962744427 1101 52-53 0.004881512824830736 1101 54-55 -0.04614767356907379 1101 56-57 0.18436708714644823 1101 58-59 0.03257191157695871 1101 60-61 -0.10556574322734491 1101 62-63 -0.4300181518583557 1101 64-65 -0.33362806421169466 1101 66-67 -0.36776204988149885 1101 68-69 -0.045760110808615195 1101 70-71 -0.331411096677229 1101 72-73 -0.4039591507899871 1101 74-75 -0.44408571951095865 1101 76 -1.3455919435074328 1102 1 -0.13181163954872943 1102 2 -0.043379082380038625 1102 3 0.07413191184695478 1102 4 -0.1473725906525445 1102 5 -0.6208688491510443 1102 6 -0.45015568842060816 1102 7 -0.7685877520227393 1102 8 -0.08546759586117503 1102 9 0.044738029363550424 1102 10-11 0.2667018288451004 1102 12-13 0.25635505468635955 1102 14-15 0.09912854898259837 1102 16-17 0.20680311758545145 1102 18-19 0.15042488880325067 1102 20-21 0.17597583108106818 1102 22-23 -0.10987315166508438 1102 24-25 0.17490901665865266 1102 26-27 -0.07591113275444883 1102 28-29 0.2965264657434332 1102 30-31 0.25014342840364634 1102 32-33 0.1266805634938848 1102 34-35 0.08885150136570985 1102 36-37 0.20961910232295367 1102 38-39 -0.03802358935407568 1102 40-41 0.12514029377826574 1102 42-43 0.12451592343740714 1102 44-45 0.11914057472318262 1102 46-47 -0.4161042731042457 1102 48-49 -0.35346920844097696 1102 50-51 -0.09718376641178139 1102 52-53 0.0677055613631623 1102 54-55 -0.04631314516311846 1102 56-57 -0.106807761171261 1102 58-59 -0.0934622858858134 1102 60-61 0.07372272891827691 1102 62-63 0.03498460600154374 1102 64-65 0.11339344709552535 1102 66-67 -0.054358850764010924 1102 68-69 0.019711483234409855 1102 70-71 0.20959828004644265 1102 72-73 0.08936682825027731 1102 74-75 -0.3994359677183468 1102 76 0.25352554132433625 1103 1 -0.44911254245274534 1103 2 -0.7912645442732824 1103 3 -0.7915517262102263 1103 4 -0.4779691683308336 1103 5 -0.11902097570784065 1103 6 -0.9770099467923785 1103 7 -0.62650431227393 1103 8 -0.8343295762406626 1103 9 -0.5147469698101617 1103 10-11 -0.7629580422544535 1103 12-13 -0.5888742769242938 1103 14-15 -0.5689753525843386 1103 16-17 -0.6417876911220901 1103 18-19 -0.1450095116895156 1103 20-21 -0.6400293069085095 1103 22-23 -0.1932302719871828 1103 24-25 -0.610850339139617 1103 26-27 -0.31459478583499134 1103 28-29 -0.620578382295264 1103 30-31 -0.8742500033827838 1103 32-33 -0.626928799064892 1103 34-35 -0.8578305892852569 1103 36-37 -0.36927607968507203 1103 38-39 -1.0131862172404382 1103 40-41 -0.48318565306907857 1103 42-43 -0.6001734685671636 1103 44-45 -0.6350058730030312 1103 46-47 -0.5445502566085949 1103 48-49 -0.6552772699766649 1103 50-51 -1.0177439875556615 1103 52-53 -0.6797329555306035 1103 54-55 -0.7735001709882781 1103 56-57 -0.7493381459195447 1103 58-59 -0.7169317809506381 1103 60-61 -0.7040291752927743 1103 62-63 -0.723728943568041 1103 64-65 -0.6720541739552033 1103 66-67 -0.5930102036177018 1103 68-69 -0.4594661379665581 1103 70-71 -0.5248638085012587 1103 72-73 -0.5850994668385852 1103 74-75 -0.6713588077345776 1103 76 -0.545131370173042 1104 1 -0.6184303521948387 1104 2 -0.5883359766520009 1104 3 -0.6918806760834428 1104 4 -0.46659298521540293 1104 5 -0.743839177600826 1104 6 -0.22198622737443685 1104 7 -0.20683813547860552 1104 8 -0.46971681572682655 1104 9 -0.2590912402191421 1104 10-11 -0.20992058613347808 1104 12-13 -0.4325564595676141 1104 14-15 -0.3611639352824838 1104 16-17 -0.35960085977743006 1104 18-19 -0.5496597359389028 1104 20-21 -0.7865909431295464 1104 22-23 -0.533512544482555 1104 24-25 -0.5321174814637288 1104 26-27 -0.495081281376649 1104 28-29 -0.4227706199920078 1104 30-31 -0.24719882578791896 1104 32-33 -0.30653200328032426 1104 34-35 -0.3099558186744886 1104 36-37 -0.2839547063193564 1104 38-39 -0.18730692356891865 1104 40-41 -0.6193590979813948 1104 42-43 -0.22892460123599534 1104 44-45 -0.25166124470876383 1104 46-47 -0.31368188985994294 1104 48-49 -0.5283250819269867 1104 50-51 -0.46835581909068225 1104 52-53 -0.2883530412707813 1104 54-55 -0.14999167692383963 1104 56-57 -0.10549872219098688 1104 58-59 -0.034383637766062236 1104 60-61 0.02756257659373773 1104 62-63 -0.21812270874961115 1104 64-65 -0.07131327528242792 1104 66-67 -0.21168153548395452 1104 68-69 -0.4641833705234646 1104 70-71 -0.1767004452933847 1104 72-73 0.02932292916528212 1104 74-75 -0.2856289121304698 1104 76 -0.10927659545040314 1105 1 0.41088563361729413 1105 2 0.2356840209018145 1105 3 0.10549502719444348 1105 4 0.5061626410076876 1105 5 0.2686325383972168 1105 6 0.14124151235159133 1105 7 0.12798711525525164 1105 8 -0.08131249209669278 1105 9 0.36434167318594746 1105 10-11 0.56139370263854 1105 12-13 -0.7176133333445236 1105 14-15 0.2746986503339528 1105 16-17 0.08372984900437075 1105 18-19 0.29636253962371484 1105 20-21 0.3119329377412967 1105 22-23 0.30952462222487753 1105 24-25 -0.03274813826412526 1105 26-27 0.26876641309497273 1105 28-29 0.2577806887164087 1105 30-31 0.31282441295962826 1105 32-33 -0.040375722703025474 1105 34-35 0.21894546251435543 1105 36-37 0.1826109278538368 1105 38-39 0.48228815654361057 1105 40-41 0.3252979011624362 1105 42-43 0.21682935852427931 1105 44-45 0.21543567004172104 1105 46-47 0.4134233572046355 1105 48-49 0.3370698238949288 1105 50-51 -0.18019534942336435 1105 52-53 -0.10324189278741613 1105 54-55 0.032961860869697546 1105 56-57 0.13608105398318315 1105 58-59 0.08291470494430797 1105 60-61 -0.010548204962034902 1105 62-63 0.22498822569266252 1105 64-65 0.11860806461483264 1105 66-67 0.5305040534348322 1105 68-69 0.19071549505873264 1105 70-71 0.3702032209248145 1105 72-73 -0.02003456533080339 1105 74-75 -0.04621821313340746 1105 76 0.03132929373746407 1106 1 -0.1311505087506113 1106 2 -0.12453949915689222 1106 3 0.268980380668026 1106 4 0.3788081889528918 1106 5 -0.236989862532333 1106 6 -0.40789620780496705 1106 7 -1.0346267851850612 1106 8 -0.3718694576054986 1106 9 -0.5134943713346019 1106 10-11 -0.34005757243484425 1106 12-13 -0.25643121494922383 1106 14-15 -0.1713400605271076 1106 16-17 -0.3008924449046333 1106 18-19 2.4241670297442397E-4 1106 20-21 0.1106693308430593 1106 22-23 -0.17539121375848765 1106 24-25 0.13617997450495523 1106 26-27 -0.09586406024161676 1106 28-29 -0.2613224790918096 1106 30-31 -0.6092340508368466 1106 32-33 -0.1470408095425526 1106 34-35 -0.24635503589561836 1106 36-37 -0.24188034232718536 1106 38-39 -0.356582853118816 1106 40-41 -0.15360567332386665 1106 42-43 -0.30426706698942496 1106 44-45 -0.26535987484574974 1106 46-47 -0.36651946324546003 1106 48-49 -0.05474386861974523 1106 50-51 -0.40084114198696597 1106 52-53 -0.20126889254279945 1106 54-55 -0.42592122682599864 1106 56-57 -0.2708607574160382 1106 58-59 -0.3925049684901367 1106 60-61 -0.5037485780050872 1106 62-63 -0.3580444308631101 1106 64-65 -0.09283969406912007 1106 66-67 -0.21853085055244748 1106 68-69 -0.1921677149461587 1106 70-71 -0.12679829265150744 1106 72-73 -0.3131428242593728 1106 74-75 -0.42163673991912276 1106 76 -0.15232943302378743 1107 1 -0.014414847151936527 1107 2 0.07528738628642984 1107 3 0.5279409406559807 1107 4 0.18813379485384019 1107 5 0.5109101826279847 1107 6 -0.528229641494562 1107 7 0.4563825479475625 1107 8 -0.24131850171207958 1107 9 0.38663734626287294 1107 10-11 0.2145337266770042 1107 12-13 0.4750549358862415 1107 14-15 0.3591337464877995 1107 16-17 0.2439912672736 1107 18-19 0.2637453521237134 1107 20-21 0.3182129858182208 1107 22-23 0.30247834818641905 1107 24-25 0.5324862367358776 1107 26-27 0.6497459804026633 1107 28-29 0.5243071310241021 1107 30-31 0.6221994129596311 1107 32-33 0.8039451907585118 1107 34-35 0.5903847653989729 1107 36-37 0.7736115288153727 1107 38-39 -0.10847205980254415 1107 40-41 0.7719475405855079 1107 42-43 0.33652587294736236 1107 44-45 0.4221514152340333 1107 46-47 0.5651661456661756 1107 48-49 0.4769436219718486 1107 50-51 0.4755738813458663 1107 52-53 0.4635549822125782 1107 54-55 0.6760658272158508 1107 56-57 0.6362312943677964 1107 58-59 0.5858293684058467 1107 60-61 0.5129193431148877 1107 62-63 0.4287141872311224 1107 64-65 0.4914896751917581 1107 66-67 0.5786861447809812 1107 68-69 0.8084588844818157 1107 70-71 0.5023997353478933 1107 72-73 0.6044395933230362 1107 74-75 0.7878412820588991 1107 76 0.29440020719900417 1108 1 -0.04762199433546144 1108 2 -0.29245943382259654 1108 3 -0.47882768441973056 1108 4 -0.023980075527745726 1108 5 0.2263250826885539 1108 6 -0.04269057426257916 1108 7 -0.0026877125006521396 1108 8 0.02715515061984064 1108 9 -0.12246319886129697 1108 10-11 0.2502393596267325 1108 12-13 0.14293046586807634 1108 14-15 0.1926058845859231 1108 16-17 0.17788739083508176 1108 18-19 0.4700445647221443 1108 20-21 0.5739746454184669 1108 22-23 0.2963986994886554 1108 24-25 0.3841594650823339 1108 26-27 0.022103642425683745 1108 28-29 0.2035545568266457 1108 30-31 0.0535970507549024 1108 32-33 0.2361529436355525 1108 34-35 -0.04104315707225936 1108 36-37 0.32796495098376255 1108 38-39 0.2959646452247142 1108 40-41 0.32258351878054725 1108 42-43 0.1516303550793907 1108 44-45 0.050581154785810156 1108 46-47 0.4110550009054208 1108 48-49 0.26449662015476605 1108 50-51 -0.07980164863595718 1108 52-53 -0.09510643511024597 1108 54-55 0.23481809856654934 1108 56-57 0.2693703010304276 1108 58-59 0.1355568063222634 1108 60-61 0.2884644672820613 1108 62-63 0.309203284747781 1108 64-65 0.25797968124868476 1108 66-67 0.2056470775490098 1108 68-69 -0.01620041291371166 1108 70-71 0.029905792283081212 1108 72-73 -0.2426985047993071 1108 74-75 0.21489306885478499 1108 76 0.5088914246429752 1109 1 0.2534233491345361 1109 2 0.23519012722365318 1109 3 0.33651005353543795 1109 4 0.25095430767435545 1109 5 0.4079629383014307 1109 6 0.8655590410872236 1109 7 0.41717683029356323 1109 8 0.510155120451202 1109 9 0.5151887756763713 1109 10-11 0.6369033111155531 1109 12-13 0.26104447316888724 1109 14-15 0.7292155325178626 1109 16-17 0.6199749100675902 1109 18-19 0.7248564632348291 1109 20-21 0.5072529209788499 1109 22-23 0.4731643193034216 1109 24-25 0.37008202217649 1109 26-27 0.40432526127504786 1109 28-29 0.36241131611105004 1109 30-31 0.561327765450045 1109 32-33 0.5967097059368243 1109 34-35 0.4807951691714436 1109 36-37 0.35435497119674864 1109 38-39 0.6290905691394002 1109 40-41 0.18199764373905936 1109 42-43 0.4645357462158515 1109 44-45 0.2563652378099235 1109 46-47 0.44792108230042516 1109 48-49 0.27800462992941277 1109 50-51 0.858118826821844 1109 52-53 0.19832509715511293 1109 54-55 0.44942273850379166 1109 56-57 0.5450325027379748 1109 58-59 0.5185364123197829 1109 60-61 0.28878878019122567 1109 62-63 0.44586346515626474 1109 64-65 0.49695681341751197 1109 66-67 0.7365737074099243 1109 68-69 0.6256450029610292 1109 70-71 0.5046433250914788 1109 72-73 0.32820911881670867 1109 74-75 0.6997370969719583 1109 76 0.2928160633734791 1110 1 -0.30036045260621336 1110 2 -0.12555002293248307 1110 3 0.3563869166453095 1110 4 -0.3617624553314265 1110 5 -0.0808639392039403 1110 6 0.10129088614735338 1110 7 -0.1212037867196969 1110 8 0.07923137230600474 1110 9 0.22372419967920365 1110 10-11 -0.17080049594045477 1110 12-13 -0.17551013006899296 1110 14-15 0.06753192345534131 1110 16-17 0.3164734815910748 1110 18-19 -0.02305618930210329 1110 20-21 0.30888847121914154 1110 22-23 0.036006212422371675 1110 24-25 0.28275116929849275 1110 26-27 0.07278029194853985 1110 28-29 0.14666706877420665 1110 30-31 -0.13187091459335676 1110 32-33 0.13600388351084547 1110 34-35 -0.11943626617061653 1110 36-37 0.2787675240725278 1110 38-39 0.08564384356191823 1110 40-41 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-0.19563513891509388 2116 5 -0.7405599688421667 2116 6 0.13671689845197932 2116 7 0.65033116689618 2116 8 0.007196496802919228 2116 9 -0.2930998084742811 2116 10-11 0.4809875431308157 2116 12-13 0.5478608461921581 2116 14-15 0.24391619377024654 2116 16-17 0.45390736468969806 2116 18-19 0.5143245027028627 2116 20-21 0.18176733687257496 2116 22-23 0.5493932576013307 2116 24-25 0.10927195102159004 2116 26-27 0.3507297938864795 2116 28-29 -0.1716202524032866 2116 30-31 0.42195810021831903 2116 32-33 0.19714388395720306 2116 34-35 0.2925194550336627 2116 36-37 0.018499411203258376 2116 38-39 0.41467911334863317 2116 40-41 -0.35555468691671166 2116 42-43 0.2625468114682974 2116 44-45 0.49655370213631755 2116 46-47 0.3734710164710435 2116 48-49 0.40907905410728773 2116 50-51 0.4182602490322367 2116 52-53 0.5290955227531242 2116 54-55 0.7403663915164103 2116 56-57 0.44628104191755114 2116 58-59 0.17391223148870694 2116 60-61 0.09495825015379467 2116 62-63 0.1151004361173733 2116 64-65 0.12015020385228325 2116 66-67 0.2776874812823209 2116 68-69 0.43573464925757577 2116 70-71 0.5329573534055143 2116 72-73 0.47064095952440255 2116 74-75 0.29650997822759706 2116 76 0.45815874595754025 2117 1 -0.023328156065247185 2117 2 -0.31112897512993243 2117 3 0.23313019584523786 2117 4 -0.5526171208970752 2117 5 -0.5150666183488148 2117 6 0.17323555997064233 2117 7 -0.3159922994272861 2117 8 -0.192020577414155 2117 9 -0.20628084665532498 2117 10-11 -0.24640949676621915 2117 12-13 -0.25171551588420726 2117 14-15 -0.239996290142237 2117 16-17 -0.6689208206384905 2117 18-19 -0.42196893359056986 2117 20-21 -0.6608163182110829 2117 22-23 -0.3290387458306796 2117 24-25 -0.6218788426292008 2117 26-27 -0.1332363150796354 2117 28-29 -0.7930005612835913 2117 30-31 -0.3149037616435493 2117 32-33 -0.8787623794490571 2117 34-35 -0.3031744531602527 2117 36-37 -0.17814366043980812 2117 38-39 -0.4439116202420976 2117 40-41 -0.7367215680835955 2117 42-43 -0.8977384738169931 2117 44-45 -0.8232393051566902 2117 46-47 -0.6746842816842502 2117 48-49 -0.5562523487241222 2117 50-51 -0.5829731272011429 2117 52-53 -0.5719608908032896 2117 54-55 -0.4351215964715678 2117 56-57 -0.866058082921576 2117 58-59 -0.9488623287858573 2117 60-61 -0.49507821488266757 2117 62-63 -0.681633798116863 2117 64-65 -0.9962697875677122 2117 66-67 -0.5122631836683453 2117 68-69 -0.9803689543460266 2117 70-71 -0.5856344526862998 2117 72-73 -1.5591745702911197 2117 74-75 -0.5744145176969013 2117 76 -0.9801724423736466 2118 1 -0.1604384450679639 2118 2 -0.38531635260416053 2118 3 -0.1984688049768728 2118 4 -0.5013697295102411 2118 5 -0.9479620208657309 2118 6 -0.3569532087639473 2118 7 -0.46953685137821566 2118 8 -0.4493325991684074 2118 9 0.0660504630265848 2118 10-11 -1.5519848145427346 2118 12-13 -0.4165363911134463 2118 14-15 -0.2955360359210246 2118 16-17 -0.05854091170279929 2118 18-19 -0.48286819369302236 2118 20-21 -0.6043161284937639 2118 22-23 -0.41939952493647326 2118 24-25 -0.3231412533039659 2118 26-27 -0.48175493899665867 2118 28-29 -0.1956400035146686 2118 30-31 -0.24637628425152514 2118 32-33 -0.3153928417269327 2118 34-35 -0.6370018420175185 2118 36-37 -0.10354522876966854 2118 38-39 -0.6026338740838852 2118 40-41 -0.43206716980369464 2118 42-43 -0.3056860485972379 2118 44-45 -0.377921370545927 2118 46-47 -0.4411738788511457 2118 48-49 -0.7180456517026741 2118 50-51 -0.39234674384567114 2118 52-53 -0.4417239022495707 2118 54-55 -0.3722026373872751 2118 56-57 -0.41565730508056475 2118 58-59 -0.3093054768286052 2118 60-61 -0.47420140466323346 2118 62-63 -0.5016987762993708 2118 64-65 -0.4730215108333766 2118 66-67 -0.6060863038858813 2118 68-69 -0.976681323915372 2118 70-71 -0.3393141782285696 2118 72-73 -0.3970927388865846 2118 74-75 -0.34280919296408285 2118 76 -0.6270294587525775 2119 1 0.5148603597129124 2119 2 0.24416571910898455 2119 3 0.1859933464175505 2119 4 -0.007345825127771377 2119 5 0.4265399711661395 2119 6 0.09125396255079465 2119 7 0.31930777013572964 2119 8 0.6940936956523132 2119 9 0.4266082319508868 2119 10-11 0.5675370978078647 2119 12-13 0.41912097940447524 2119 14-15 0.23036037842160084 2119 16-17 0.28309255443265613 2119 18-19 -0.03964110205954796 2119 20-21 0.26520578385683535 2119 22-23 0.3555008734699001 2119 24-25 0.1927950016761173 2119 26-27 0.4843805191706636 2119 28-29 0.4368301160072434 2119 30-31 0.27852411415484823 2119 32-33 0.2383920188308437 2119 34-35 0.17574716196654805 2119 36-37 0.18231134493551338 2119 38-39 0.2579238948404239 2119 40-41 0.10578143577399146 2119 42-43 0.5210071784944006 2119 44-45 0.1798475537568649 2119 46-47 0.15842771477436202 2119 48-49 0.3048627148311809 2119 50-51 0.06482457089536098 2119 52-53 -0.2903294799388121 2119 54-55 -0.007660804717950498 2119 56-57 -0.18059754412438878 2119 58-59 -0.05731344495608681 2119 60-61 0.12042010928099245 2119 62-63 0.03515381334207035 2119 64-65 -0.051706769797519314 2119 66-67 -0.10409427201337706 2119 68-69 -0.2794701685368821 2119 70-71 0.10391291340489062 2119 72-73 0.27023793840425725 2119 74-75 0.29065694249408836 2119 76 0.8650024137573809 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 10.0 13 255.0 14 1215.0 15 2414.0 16 3253.0 17 3628.0 18 3698.0 19 3320.0 20 2646.0 21 1951.0 22 1491.0 23 1275.0 24 1270.0 25 1341.0 26 1439.0 27 1708.0 28 2050.0 29 2344.0 30 3012.0 31 3923.0 32 4899.0 33 6445.0 34 9074.0 35 13532.0 36 25769.0 37 92263.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.33911866012449 17.274117398713877 10.000154458442957 30.386609482718676 2 22.44178203854253 20.94096083448748 28.925947473832164 27.691309653137825 3 19.891158283863728 19.54311192573638 26.464909616067793 34.1008201743321 4 15.618322890226386 16.723215618837752 31.247457871459684 36.411003619476176 5 16.100748093725382 30.255834667682656 33.00107606048593 20.64234117810603 6 30.530770696144206 30.62087145453516 19.294948694053865 19.553409155266777 7 31.613172283834295 25.2406989795393 19.270334558709955 23.875794177916447 8 26.508157980095454 29.51803817182987 20.641826316629512 23.331977531445165 9 24.994465239127415 16.564123422593152 19.512220237145193 38.92919110113424 10-11 26.999405333463766 21.994424035855044 24.568995476930287 26.437175153750903 12-13 25.965493983843647 19.80981017057361 24.3421357483769 29.882560097205847 14-15 24.78697606408996 20.835156801062674 22.208807220417345 32.16905991443002 16-17 26.07773378572495 23.095656113722605 22.80424451801243 28.022365582540015 18-19 25.214311089601342 22.432257101226917 23.529169476952223 28.82426233221952 20-21 24.919295463555528 22.698955346064142 23.986366468101757 28.395382722278573 22-23 26.23502396680173 22.490951309550166 23.151776014663255 28.12224870898485 24-25 25.712182137395935 23.293877782182705 22.614003202438386 28.37993687798298 26-27 24.481663208513748 23.611804743933646 24.01854531038424 27.88798673716836 28-29 25.198092953090974 23.002466186472528 24.806283369459447 26.993157490977055 30-31 25.330154921818288 22.582081790894158 24.584635503817697 27.503127783469854 32-33 24.237876299381654 23.576536732792043 24.573308551334264 27.612278416492043 34-35 24.625824421939278 23.128607248219865 24.745787145968375 27.49978118387248 36-37 24.849789164225367 22.10351804846906 24.892265236038245 28.15442755126733 38-39 23.928187121255025 22.36043392525241 26.527722716203208 27.183656237289355 40-41 23.695273302046836 21.795429562907223 26.67478433684384 27.8345127982021 42-43 22.845948297610526 21.229283261338537 26.78026227043614 29.144506170614797 44-45 22.1462515510202 20.924227836500588 28.663882982283617 28.265637630195595 46-47 21.914049025109794 21.761135166583433 28.87806535651583 27.446750451790948 48-49 21.330710972212927 22.10892409397252 28.51714746147549 28.043217472339066 50-51 21.191440942814335 23.22231203694646 27.485879924006447 28.10036709623276 52-53 20.169698342660908 23.37574075694934 27.565940883605265 28.888620016784483 54-55 20.232511442796316 22.99500069506299 27.567742898773083 29.204744963367606 56-57 20.276532099038754 23.340730176546 27.21943910990748 29.163298614507767 58-59 20.745570904148238 23.051892888218426 26.686557481709546 29.51597872592379 60-61 20.65778702240162 22.492238463241463 27.066010389904594 29.783964124452318 62-63 20.98246119865209 22.937652688999698 26.277052822349162 29.802833289999047 64-65 21.606161862150987 22.852641496805283 24.898701004494743 30.64249563654899 66-67 21.731015770207026 23.394790631580573 23.85868082192486 31.01551277628754 68-69 21.52744347450013 23.759631152288694 23.882683361951123 30.83024201126005 70-71 22.218332157732963 24.526456156970966 23.393760908627534 29.861450776668537 72-73 22.49306865952895 25.071244140217736 22.829788931994347 29.60589826825897 74-75 22.118505971121348 25.961699356164065 23.39896975953333 28.520824913181258 76 22.55980146839249 25.336463707227665 23.346513854993667 28.757220969386175 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 0.5 8 0.0 9 1.0 10 2.5 11 1.5 12 0.0 13 1.0 14 2.5 15 3.5 16 9.5 17 15.0 18 17.0 19 28.5 20 40.0 21 42.0 22 65.0 23 101.5 24 161.0 25 207.0 26 276.0 27 437.0 28 634.0 29 739.0 30 905.0 31 1183.5 32 1653.0 33 2010.0 34 2272.0 35 2912.0 36 3609.5 37 3929.0 38 4530.0 39 5700.0 40 6915.5 41 8045.0 42 8528.0 43 8565.0 44 9225.5 45 9971.5 46 10094.0 47 10149.0 48 9924.0 49 9315.0 50 8986.0 51 8496.5 52 7360.0 53 6301.5 54 5890.0 55 5594.5 56 5140.5 57 4838.0 58 4694.0 59 4524.0 60 4451.5 61 4504.5 62 4460.0 63 4453.0 64 4481.0 65 4565.5 66 4622.5 67 4630.0 68 4502.5 69 4297.0 70 4080.0 71 3941.0 72 3828.5 73 3426.0 74 2741.0 75 2346.0 76 2080.0 77 1489.0 78 897.0 79 630.0 80 451.5 81 192.0 82 82.5 83 54.0 84 40.0 85 19.5 86 14.0 87 15.0 88 11.0 89 6.5 90 5.0 91 4.0 92 4.0 93 4.0 94 4.0 95 4.5 96 5.0 97 8.5 98 9.5 99 20.5 100 34.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 5.148614765197423E-4 8 0.0 9 0.0 10-11 2.5743073825987116E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.5743073825987116E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.5743073825987116E-4 64-65 0.0 66-67 0.0 68-69 2.5743073825987116E-4 70-71 0.0 72-73 2.5743073825987116E-4 74-75 2.5743073825987116E-4 76 5.148614765197423E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 194227.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.62740645526541 #Duplication Level Percentage of deduplicated Percentage of total 1 68.87874296838974 39.6930331716715 2 17.75539602666437 20.463948472055844 3 6.270031884319911 10.839770276555315 4 2.5137243021569 5.794376483074962 5 1.2671017758877285 3.650989452963537 6 0.784955356123105 2.714096481392626 7 0.5500193733355615 2.218733298983514 8 0.40135854161090406 1.8503401449363295 9 0.3091935713779328 1.603622125003612 >10 1.2674941921064447 11.111696742622449 >50 0.001982008027405322 0.05939335074030035 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005148614765197424 5.148614765197423E-4 2 0.0 0.0 0.0 0.005663476241717166 5.148614765197423E-4 3 0.0 0.0 0.0 0.007722922147796136 5.148614765197423E-4 4 0.0 0.0 0.0 0.018020151678190984 5.148614765197423E-4 5 0.0 0.0 0.0 0.023168766443388406 5.148614765197423E-4 6 0.0 0.0 0.0 0.02368362791990815 5.148614765197423E-4 7 0.0 0.0 0.0 0.025228212349467375 5.148614765197423E-4 8 0.0 0.0 0.0 0.027802519732066086 5.148614765197423E-4 9 0.0 0.0 0.0 0.030891688591184543 5.148614765197423E-4 10 0.0 0.0 0.0 0.03552544187986222 5.148614765197423E-4 11 0.0 0.0 0.0 0.03604030335638197 5.148614765197423E-4 12 0.0 0.0 0.0 0.03809974926246094 5.148614765197423E-4 13 0.0 0.0 0.0 0.03964433369202016 5.148614765197423E-4 14 0.0 0.0 0.0 0.04170377959809913 5.148614765197423E-4 15 0.0 0.0 0.0 0.04530780993373733 5.148614765197423E-4 16 0.0 0.0 0.0 0.0473672558398163 5.148614765197423E-4 17 0.0 0.0 0.0 0.04839697879285578 5.148614765197423E-4 18 0.0 0.0 0.0 0.05251587060501372 5.148614765197423E-4 19 0.0 0.0 0.0 0.060238792752809855 5.148614765197423E-4 20 0.0 0.0 0.0 0.06693199194756651 5.148614765197423E-4 21 0.0 0.0 0.0 0.0705360222832047 5.148614765197423E-4 22 0.0 0.0 0.0 0.08031839033707981 5.148614765197423E-4 23 0.0 0.0 0.0 0.08546700510227723 5.148614765197423E-4 24 0.0 0.0 0.0 0.09370478872659312 5.148614765197423E-4 25 0.0 0.0 0.0 0.10554660268654718 5.148614765197423E-4 26 0.0 0.0 0.0 0.1215073084586592 5.148614765197423E-4 27 0.0 0.0 0.0 0.21109320537309437 5.148614765197423E-4 28 0.0 0.0 0.0 0.3289964834961154 5.148614765197423E-4 29 0.0 0.0 0.0 0.4510186534312943 5.148614765197423E-4 30 0.0 0.0 0.0 0.5745854077960325 5.148614765197423E-4 31 0.0 0.0 0.0 0.7069048072616063 5.148614765197423E-4 32 0.0 0.0 0.0 0.8690861723653251 5.148614765197423E-4 33 0.0 0.0 0.0 1.0178811390795306 5.148614765197423E-4 34 0.0 0.0 0.0 1.2737672929098427 5.148614765197423E-4 35 0.0 0.0 0.0 1.614605590365912 5.148614765197423E-4 36 0.0 0.0 0.0 2.1490318029934046 5.148614765197423E-4 37 0.0 0.0 0.0 2.909482203813064 5.148614765197423E-4 38 0.0 0.0 0.0 3.677140665304 5.148614765197423E-4 39 0.0 0.0 0.0 4.598227846797819 5.148614765197423E-4 40 0.0 0.0 0.0 5.605296894870435 5.148614765197423E-4 41 0.0 0.0 0.0 6.780725645765007 5.148614765197423E-4 42 0.0 0.0 0.0 8.089503519078193 5.148614765197423E-4 43 0.0 0.0 0.0 9.437410864606878 5.148614765197423E-4 44 0.0 0.0 0.0 10.634463797515279 5.148614765197423E-4 45 0.0 0.0 0.0 11.757891539281356 5.148614765197423E-4 46 0.0 0.0 0.0 12.819021042388545 5.148614765197423E-4 47 0.0 0.0 0.0 13.872942484824458 5.148614765197423E-4 48 0.0 0.0 0.0 14.82749566229206 5.148614765197423E-4 49 0.0 0.0 0.0 15.733651860966807 5.148614765197423E-4 50 0.0 0.0 0.0 16.565668007022712 5.148614765197423E-4 51 0.0 0.0 0.0 17.573251916571845 5.148614765197423E-4 52 0.0 0.0 0.0 18.761037342902892 5.148614765197423E-4 53 0.0 0.0 0.0 19.993100856214635 5.148614765197423E-4 54 0.0 0.0 0.0 21.08409232495997 5.148614765197423E-4 55 0.0 0.0 0.0 21.96450544980873 5.148614765197423E-4 56 0.0 0.0 0.0 22.799610764723752 5.148614765197423E-4 57 0.0 0.0 0.0 23.583744793463318 5.148614765197423E-4 58 0.0 0.0 0.0 24.312788644215274 5.148614765197423E-4 59 0.0 0.0 0.0 25.044406802349826 5.148614765197423E-4 60 0.0 0.0 0.0 25.762638562094867 5.148614765197423E-4 61 0.0 0.0 0.0 26.451008356201765 5.148614765197423E-4 62 0.0 0.0 0.0 27.185715683195436 5.148614765197423E-4 63 0.0 0.0 0.0 27.988899586566234 5.148614765197423E-4 64 0.0 0.0 0.0 28.846658806448126 5.148614765197423E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTAAT 15 0.0022221138 70.0 9 GACCTAG 15 0.0022221138 70.0 1 CGGAATA 20 0.0069433837 52.5 69 GATACTT 20 0.0069433837 52.5 18 TGCATAT 20 0.0069433837 52.5 6 AGAGGGT 50 1.7052644E-4 35.0 6 GGCTATA 40 0.0024145024 35.0 1 ATAAGAG 75 1.483395E-6 32.666668 3 GCGATGT 45 0.0042997343 31.111113 19 CAGCTAC 45 0.0042997343 31.111113 1 TAAGAGA 85 3.9367605E-6 28.82353 4 GTATAAG 75 5.564538E-5 28.0 1 CGTATCC 50 0.007195854 27.999998 56 CAGCCGT 50 0.007195854 27.999998 56 TTCGGTC 50 0.007195854 27.999998 28 TGAATGG 50 0.007195854 27.999998 41 CATTAAA 90 6.141003E-6 27.222223 61 AGAGACA 90 6.141003E-6 27.222223 6 TATAAGA 80 8.634314E-5 26.25 2 AAGAGAC 95 9.3454655E-6 25.789473 5 >>END_MODULE