FastQCFastQC Report
Wed 25 May 2016
SRR1780284_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780284_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194227
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT106065.460620819968388TruSeq Adapter, Index 5 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC30231.5564262435191811RNA PCR Primer, Index 5 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG28761.480741606470779TruSeq Adapter, Index 5 (95% over 22bp)
CACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTG27511.4163839219058112TruSeq Adapter, Index 5 (96% over 29bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC6850.3526801114160235RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT3520.1812312397349493TruSeq Adapter, Index 5 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT2790.14364635194900813No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATA150.002222113870.04
GCAACAA150.002222113870.044
CATAGAT150.002222113870.034
GAGTTTC150.002222113870.069
TATGTCG252.3860755E-455.99999636
TCTTTAT950.055.2631571
TCGTTGT200.006943383752.534
CTGTATA200.006943383752.59
ACCGATT200.006943383752.570
AAGACCG200.006943383752.541
AACGACC200.006943383752.555
CAATACG200.006943383752.569
GCAGTAT305.867276E-446.66666442
TTGCGAG305.867276E-446.6666649
CTTTATA1200.043.752
GTTGTAC350.00125319440.08
TTTATAC1500.037.3333323
TTGATAT400.002414502435.022
TGGCGTA400.002414502435.062
ATAGCAT400.002414502435.03
Produced by FastQC (version 0.11.5)