##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780284_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 194227 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.76456414401705 34.0 34.0 34.0 34.0 34.0 2 33.7550752470048 34.0 34.0 34.0 34.0 34.0 3 33.76458988709088 34.0 34.0 34.0 34.0 34.0 4 33.788875902938315 34.0 34.0 34.0 34.0 34.0 5 33.77359996292997 34.0 34.0 34.0 34.0 34.0 6 37.55770309998095 38.0 38.0 38.0 37.0 38.0 7 37.55992215294475 38.0 38.0 38.0 37.0 38.0 8 37.47760610007877 38.0 38.0 38.0 37.0 38.0 9 37.48502525395542 38.0 38.0 38.0 37.0 38.0 10-11 37.43923347423376 38.0 38.0 38.0 37.0 38.0 12-13 37.41995963486024 38.0 38.0 38.0 37.0 38.0 14-15 37.35726237855705 38.0 38.0 38.0 37.0 38.0 16-17 37.38959567928249 38.0 38.0 38.0 37.0 38.0 18-19 37.34706812132195 38.0 38.0 38.0 36.5 38.0 20-21 37.37237098858552 38.0 38.0 38.0 37.0 38.0 22-23 37.35351676131537 38.0 38.0 38.0 37.0 38.0 24-25 37.35223733054622 38.0 38.0 38.0 36.5 38.0 26-27 37.259644642608905 38.0 38.0 38.0 36.0 38.0 28-29 37.253100753242336 38.0 38.0 38.0 36.0 38.0 30-31 37.24207756903005 38.0 38.0 38.0 36.0 38.0 32-33 37.15256117841494 38.0 38.0 38.0 36.0 38.0 34-35 37.16570816621788 38.0 38.0 38.0 36.0 38.0 36-37 37.196728570178195 38.0 38.0 38.0 36.0 38.0 38-39 36.65742404506068 38.0 38.0 38.0 34.0 38.0 40-41 36.90191116580084 38.0 38.0 38.0 35.5 38.0 42-43 37.13885299160261 38.0 38.0 38.0 36.0 38.0 44-45 37.19470516457547 38.0 38.0 38.0 36.0 38.0 46-47 37.15569153619219 38.0 38.0 38.0 36.0 38.0 48-49 37.16909338042599 38.0 38.0 38.0 36.0 38.0 50-51 37.05266260612582 38.0 38.0 38.0 36.0 38.0 52-53 37.051447533041234 38.0 38.0 38.0 36.0 38.0 54-55 37.02817579430254 38.0 38.0 38.0 36.0 38.0 56-57 37.07431510552086 38.0 38.0 38.0 36.0 38.0 58-59 36.98131052840233 38.0 38.0 38.0 36.0 38.0 60-61 36.98136201454999 38.0 38.0 38.0 36.0 38.0 62-63 36.954038315991085 38.0 38.0 38.0 36.0 38.0 64-65 36.87785683761784 38.0 38.0 38.0 35.5 38.0 66-67 36.60606918708521 38.0 37.5 38.0 34.5 38.0 68-69 36.52749360284615 38.0 38.0 38.0 34.0 38.0 70-71 35.93376049673836 38.0 37.0 38.0 31.5 38.0 72-73 34.968541963784645 38.0 37.0 38.0 24.0 38.0 74-75 34.67661035798318 38.0 37.0 38.0 23.0 38.0 76 33.99536109809656 38.0 35.0 38.0 22.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.10276036980574332 1101 2 -0.2028616035056956 1101 3 -0.08173756061476922 1101 4 0.024166265087785632 1101 5 -0.057471989915320876 1101 6 -0.17051040418706975 1101 7 -0.09314068943467646 1101 8 -0.1715835978096294 1101 9 -0.04936983940627471 1101 10-11 0.02831185692117799 1101 12-13 -0.024431066128457246 1101 14-15 0.013784576344164634 1101 16-17 0.08958943679998299 1101 18-19 -0.0019498928485930378 1101 20-21 0.10296304154710612 1101 22-23 -0.11592611834438316 1101 24-25 0.03548288168566671 1101 26-27 -0.030277231174977715 1101 28-29 -0.08468559334080794 1101 30-31 -0.018877825247571423 1101 32-33 -0.07953464712294789 1101 34-35 0.044415346363116726 1101 36-37 0.057033659918651836 1101 38-39 0.10036899170319913 1101 40-41 0.03570922002740673 1101 42-43 -0.03295941817919612 1101 44-45 -0.1342603616938831 1101 46-47 -0.07503686180188396 1101 48-49 -0.057270988428896885 1101 50-51 -0.23038368187775404 1101 52-53 -0.08227013714557785 1101 54-55 -0.11276969795940062 1101 56-57 0.02328307085490877 1101 58-59 0.11580357234684868 1101 60-61 -0.12204199523132075 1101 62-63 -0.10753858318656029 1101 64-65 0.05770966132756428 1101 66-67 -0.2845479788163914 1101 68-69 -0.685035465938725 1101 70-71 -0.2521630539183164 1101 72-73 -0.22971309973746656 1101 74-75 0.07489368638651683 1101 76 -0.9526405535045939 1102 1 -0.03298651431760646 1102 2 0.030618815689010148 1102 3 -0.06883077627941248 1102 4 0.04920763298629538 1102 5 -0.09569592813925709 1102 6 -0.13493401146781991 1102 7 -0.08525321011862275 1102 8 -0.2034644582619194 1102 9 -0.09884584602514224 1102 10-11 0.006248977715443971 1102 12-13 -0.07114921284659914 1102 14-15 0.08899141583672332 1102 16-17 -0.04998585277530765 1102 18-19 -0.047344266814391744 1102 20-21 0.01029894888301186 1102 22-23 0.005667924678228076 1102 24-25 -0.039034492831710565 1102 26-27 -0.02084535031453072 1102 28-29 0.06983729689636675 1102 30-31 0.020642307129705273 1102 32-33 0.01817632915945211 1102 34-35 0.11896029947950382 1102 36-37 -0.030969649513231445 1102 38-39 -0.04816934254942851 1102 40-41 -0.0636288935964231 1102 42-43 0.028265071616722537 1102 44-45 0.09113952799172154 1102 46-47 -0.06441910118412153 1102 48-49 0.09876872475366838 1102 50-51 -0.044917603554523566 1102 52-53 0.16480653135965895 1102 54-55 -0.04098260474373916 1102 56-57 -0.031074347788219825 1102 58-59 0.32256032910360233 1102 60-61 0.2386033439854529 1102 62-63 0.07914095349297412 1102 64-65 0.2870532906711958 1102 66-67 0.18023415025145084 1102 68-69 0.0959628793453362 1102 70-71 0.0044006526453870265 1102 72-73 -0.19893538324546256 1102 74-75 -0.15971745536748472 1102 76 0.2297091431308118 1103 1 -0.06027645016785499 1103 2 0.06219040951741306 1103 3 -0.018615191044368373 1103 4 -0.09983913473751471 1103 5 0.020682540379290515 1103 6 -0.046115899303401875 1103 7 0.01178268069158861 1103 8 -0.0457041409685246 1103 9 -0.13463149270572927 1103 10-11 -0.0543928202804409 1103 12-13 -0.11120149414401936 1103 14-15 0.06332400300977525 1103 16-17 0.048788992108491414 1103 18-19 0.15339037555448698 1103 20-21 0.10073958407073036 1103 22-23 0.10667373871905994 1103 24-25 0.022670207978045198 1103 26-27 0.037347639785082265 1103 28-29 0.015858359648952103 1103 30-31 -0.01725787765764153 1103 32-33 0.14202891993811306 1103 34-35 -0.019874338382372514 1103 36-37 0.07440045099305337 1103 38-39 -0.07143677340440036 1103 40-41 0.06649219469587564 1103 42-43 -0.10555927524264774 1103 44-45 -0.004871018407925476 1103 46-47 -0.06864442155730188 1103 48-49 -0.1503862111716927 1103 50-51 -0.1867681812027655 1103 52-53 0.07001287722748373 1103 54-55 -0.05478907380312137 1103 56-57 -0.1620818143987961 1103 58-59 -0.1430624643402041 1103 60-61 -0.10122884506853325 1103 62-63 -0.028550256338313318 1103 64-65 -0.03948322360851364 1103 66-67 -0.4394832603219925 1103 68-69 -0.26220532220808224 1103 70-71 -0.4293726147854713 1103 72-73 -0.5202475236237518 1103 74-75 -0.6861322804204377 1103 76 -0.9026842285621655 1104 1 6.782659225166299E-4 1104 2 -0.09922727306118873 1104 3 -0.05529081753397236 1104 4 -0.10943731332988449 1104 5 -0.03711973942608182 1104 6 -0.02555653222732701 1104 7 -0.08861175457305848 1104 8 -0.29953998584429087 1104 9 -0.06399103034840437 1104 10-11 -0.05989054732818744 1104 12-13 -0.030106212899490004 1104 14-15 -0.014409440988785605 1104 16-17 0.010137382005467543 1104 18-19 -0.053096104758012075 1104 20-21 -0.04821112675035266 1104 22-23 -0.027639844930909874 1104 24-25 -0.044654104615702295 1104 26-27 -0.005004656391641049 1104 28-29 -0.24974010829747328 1104 30-31 -0.24886105141474957 1104 32-33 -0.19971915928397976 1104 34-35 -0.11490810904644633 1104 36-37 -0.05708431603886055 1104 38-39 -0.3349678827726237 1104 40-41 -0.04715351410734314 1104 42-43 -0.2716370810251547 1104 44-45 -0.13779684478026866 1104 46-47 -0.25116210642027426 1104 48-49 -0.12503727302492962 1104 50-51 -0.29021035227193437 1104 52-53 -0.22294668173601906 1104 54-55 -0.5188082777748875 1104 56-57 -0.5510669431232813 1104 58-59 -0.25758194624415154 1104 60-61 -0.13919323929058436 1104 62-63 -0.25499455187541287 1104 64-65 -0.39855256959219787 1104 66-67 -0.7068711546620676 1104 68-69 -0.14434494315427315 1104 70-71 -0.4590123689868122 1104 72-73 -0.44848581430274237 1104 74-75 -0.14432633038731524 1104 76 -0.3658639250449909 1105 1 -0.008651632560848554 1105 2 0.04202838373920059 1105 3 -0.06840847898211422 1105 4 -0.044376464504054525 1105 5 0.021936492227538906 1105 6 7.667004047675618E-4 1105 7 -0.0797558042305937 1105 8 -0.05769648046268827 1105 9 0.0503910024304588 1105 10-11 -0.07139716731351342 1105 12-13 0.034553308871544175 1105 14-15 -0.007277285900727293 1105 16-17 0.11389189215712747 1105 18-19 -0.03608174871593661 1105 20-21 -0.048822672738609185 1105 22-23 0.010795820431120262 1105 24-25 -0.09504167188576673 1105 26-27 0.05799604688624527 1105 28-29 0.12331823318980639 1105 30-31 -0.011527697696550376 1105 32-33 -0.032623773398462674 1105 34-35 -0.08278092276953686 1105 36-37 -0.07005100207114623 1105 38-39 -0.30830447768455826 1105 40-41 -0.12012623915627074 1105 42-43 -0.06827510695470806 1105 44-45 -0.21896619715306542 1105 46-47 -0.10935605823045336 1105 48-49 -0.05669700883706241 1105 50-51 -0.25168868825529955 1105 52-53 -0.009677662655782626 1105 54-55 -0.3098200806124609 1105 56-57 -0.18001332582876017 1105 58-59 0.01739397413255972 1105 60-61 0.14825057492173954 1105 62-63 -0.01613233318656171 1105 64-65 -0.058668686886719 1105 66-67 -0.416547341061289 1105 68-69 -0.1714154085407671 1105 70-71 -0.35436729498974984 1105 72-73 -0.24138003213542447 1105 74-75 -0.2683421235624621 1105 76 -0.2382113443209235 1106 1 -0.12065245814793002 1106 2 -0.028777175213832606 1106 3 0.03081485761279623 1106 4 -0.03898721548252837 1106 5 -0.02146416212666935 1106 6 0.07229082589400093 1106 7 -0.06904228294879289 1106 8 0.1349022842144123 1106 9 0.1708426291427898 1106 10-11 0.17787853290663946 1106 12-13 0.042300832110292674 1106 14-15 0.036471185234304926 1106 16-17 0.13244657965712747 1106 18-19 0.21304870933200704 1106 20-21 0.007561867378811371 1106 22-23 0.060422777378285275 1106 24-25 0.16180382102029967 1106 26-27 -0.024574128015906638 1106 28-29 0.060436016947150506 1106 30-31 0.006520553281923469 1106 32-33 0.0830597056866651 1106 34-35 0.0651310298968042 1106 36-37 0.18557713210204696 1106 38-39 0.10730217593579994 1106 40-41 -0.08922787809952126 1106 42-43 0.1755253455893282 1106 44-45 0.1410681258655231 1106 46-47 0.05412165091827603 1106 48-49 0.1890527465445473 1106 50-51 0.022901193716322155 1106 52-53 0.19486153744206547 1106 54-55 0.3305067907182675 1106 56-57 0.2874849159765276 1106 58-59 0.14359222205330013 1106 60-61 0.17489678027887834 1106 62-63 0.2176081878506153 1106 64-65 0.16798363393030513 1106 66-67 0.22854958897784883 1106 68-69 0.2969466419729301 1106 70-71 0.08501894216778538 1106 72-73 -0.47881849532035403 1106 74-75 -0.5132108705047358 1106 76 -0.21126509921329273 1107 1 -0.028152834483925915 1107 2 0.03905362412381663 1107 3 -0.10614886359749676 1107 4 0.038255747034405374 1107 5 -0.13879067123399835 1107 6 0.03078472925091802 1107 7 0.09178866692325016 1107 8 0.23971938492192635 1107 9 0.1246097524304588 1107 10-11 -0.05127997981351484 1107 12-13 0.13518431848692813 1107 14-15 0.04686934871465809 1107 16-17 0.1689850411955902 1107 18-19 0.15633111186098603 1107 20-21 0.029061942646009697 1107 22-23 0.1639208204311231 1107 24-25 0.11075760696039083 1107 26-27 0.1484708065016349 1107 28-29 0.22920765626672335 1107 30-31 0.11966220614959866 1107 32-33 0.12427226025538829 1107 34-35 0.14094203396123106 1107 36-37 0.17897243542885377 1107 38-39 0.3772724453923644 1107 40-41 0.4167727993052708 1107 42-43 0.2843090276606759 1107 44-45 0.26695627880847184 1107 46-47 0.24776533600031314 1107 48-49 0.17575491423986023 1107 50-51 0.06845854732162593 1107 52-53 0.08425262580575321 1107 54-55 0.21391790015676548 1107 56-57 -0.03892258063645215 1107 58-59 0.14355082509409556 1107 60-61 -0.010268054885948175 1107 62-63 0.11457680142882509 1107 64-65 0.19026460638251308 1107 66-67 0.31295987047717233 1107 68-69 0.1686537020361527 1107 70-71 0.2509962867410209 1107 72-73 0.7089054245953434 1107 74-75 0.6018952562452284 1107 76 0.49033432875599914 1108 1 0.05251039991946982 1108 2 0.026818615038415317 1108 3 0.01586809582103399 1108 4 -0.024660667259958302 1108 5 -0.21371495954411301 1108 6 -0.23900630648499543 1108 7 0.04406001761979894 1108 8 -0.007161293454814199 1108 9 -0.07913040504985958 1108 10-11 -0.1560043892623284 1108 12-13 -0.1291554755772708 1108 14-15 -0.04089564219994202 1108 16-17 0.04386390249177197 1108 18-19 -0.07402927578286977 1108 20-21 0.017614335135405668 1108 22-23 8.302692500237185E-4 1108 24-25 -0.17773421617710028 1108 26-27 0.05745778409097113 1108 28-29 -0.030286589644845208 1108 30-31 0.03550109167352389 1108 32-33 -0.18034615282759603 1108 34-35 -0.19630054629315907 1108 36-37 0.025429128342238982 1108 38-39 -0.09669030445621019 1108 40-41 0.07407897737916613 1108 42-43 0.09181962729726223 1108 44-45 0.022982929323312362 1108 46-47 -0.0322922048839942 1108 48-49 -0.09151492222288482 1108 50-51 -0.08188215528285525 1108 52-53 -0.19850025123846393 1108 54-55 -0.1930324329746682 1108 56-57 -0.22614244738387868 1108 58-59 -0.03683984722570699 1108 60-61 -0.06146429958613453 1108 62-63 -0.11305039421018392 1108 64-65 0.13479065120382927 1108 66-67 0.02848833807257023 1108 68-69 -0.15107676434784878 1108 70-71 0.13895055688033153 1108 72-73 0.020777017569301393 1108 74-75 0.0013198474021081097 1108 76 0.4351707915058469 1109 1 0.08009208151960934 1109 2 -0.03112026329144868 1109 3 0.021569969293750546 1109 4 -0.10288041565348038 1109 5 0.01576282125052586 1109 6 -0.189823876702512 1109 7 0.05099880544373292 1109 8 -0.1863931998113486 1109 9 -0.03487300619023159 1109 10-11 -0.023119060273280923 1109 12-13 -0.024047924366009 1109 14-15 -0.12615599758655094 1109 16-17 0.032829721419581404 1109 18-19 0.058637330546510213 1109 20-21 0.05438613952192384 1109 22-23 -0.006625156580376768 1109 24-25 0.13583865617936652 1109 26-27 0.02051251000884946 1109 28-29 -0.05229838558413746 1109 30-31 0.008153217054406525 1109 32-33 0.2257237780862127 1109 34-35 0.020035019519731634 1109 36-37 -0.11460994005007308 1109 38-39 0.10117828093613213 1109 40-41 0.08602198881307999 1109 42-43 0.001992493620001312 1109 44-45 0.030506234599812387 1109 46-47 0.024032651060728938 1109 48-49 -0.10608637665315257 1109 50-51 -0.029762053916215336 1109 52-53 0.09148074251663019 1109 54-55 -0.03505056432893383 1109 56-57 0.15156518829959253 1109 58-59 0.11629618318428214 1109 60-61 0.136445767689942 1109 62-63 -0.014051993148243014 1109 64-65 0.044409168716725844 1109 66-67 0.12761872694637333 1109 68-69 0.14117064869103046 1109 70-71 0.44876743321906076 1109 72-73 -0.3231605936775708 1109 74-75 0.2820013570409827 1109 76 0.33133786544919275 1110 1 0.023384885984434334 1110 2 -0.03259303365000932 1110 3 0.12056671369611394 1110 4 0.09959782499498004 1110 5 0.010846204414434624 1110 6 -0.07599976756247884 1110 7 -0.05532857036739358 1110 8 0.2565120335064819 1110 9 0.035255224492118487 1110 10-11 -0.012937012568812634 1110 12-13 -0.013063050714201552 1110 14-15 -0.003401141873752067 1110 16-17 0.09776449873227477 1110 18-19 0.1707782735624832 1110 20-21 0.1743227993230505 1110 22-23 0.03388710174133536 1110 24-25 0.1617284087982398 1110 26-27 -0.056929741890250796 1110 28-29 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50-51 0.058387697119194115 2115 52-53 0.14285586467214983 2115 54-55 0.25440373011628736 2115 56-57 0.22899239912063507 2115 58-59 0.09183017732081566 2115 60-61 0.2202987467334836 2115 62-63 0.23881769211708104 2115 64-65 0.2343435537509322 2115 66-67 0.4308242429468123 2115 68-69 0.3407184793640923 2115 70-71 0.17290924220660742 2115 72-73 0.20505927074918873 2115 74-75 0.1429883736541342 2115 76 0.5165489036547868 2116 1 0.1253146439835504 2116 2 0.18891997399016702 2116 3 0.04892984148120405 2116 4 0.114844698623358 2116 5 0.018203685760354915 2116 6 0.12375324721944025 2116 7 0.08270045783504543 2116 8 0.1691224143249599 2116 9 0.08262133544204175 2116 10-11 0.1597238811903452 2116 12-13 0.09776970607232016 2116 14-15 0.22026554711085566 2116 16-17 -0.10403990682935671 2116 18-19 -4.6969517093486957E-5 2116 20-21 0.05276999135405447 2116 22-23 0.012424681434985985 2116 24-25 0.044942341145116416 2116 26-27 0.25135542188624527 2116 28-29 0.2078681849272641 2116 30-31 -0.057543021055622035 2116 32-33 -0.11309780211467313 2116 34-35 -0.10401267349347876 2116 36-37 -0.14776501630859684 2116 38-39 0.06959127521119512 2116 40-41 0.040618210650684716 2116 42-43 0.16533070868235455 2116 44-45 0.03708547393766537 2116 46-47 0.1604843737193491 2116 48-49 0.1769540529389957 2116 50-51 0.22245691381998967 2116 52-53 0.05476792132105146 2116 54-55 0.2466621829010549 2116 56-57 0.16197584526197062 2116 58-59 0.25209700864028406 2116 60-61 -0.017188161806060975 2116 62-63 0.1988320731840929 2116 64-65 0.1509529045708149 2116 66-67 0.18023415025145084 2116 68-69 0.20214048552293917 2116 70-71 0.29108018932492286 2116 72-73 0.396624462314378 2116 74-75 0.5980045523545243 2116 76 0.4768133902350584 2117 1 -0.16115039248148122 2117 2 -0.02831511824492594 2117 3 -0.03145411390274688 2117 4 -0.042330333551674926 2117 5 0.08459150214817157 2117 6 0.07693857540476756 2117 7 0.07460307473765937 2117 8 -0.2732842780817393 2117 9 -0.0599140570933514 2117 10-11 -0.04354188457541852 2117 12-13 0.00314829895091151 2117 14-15 0.02019047203577884 2117 16-17 -0.02429945208890416 2117 18-19 0.03920656973644299 2117 20-21 -0.04981473623067245 2117 22-23 0.14050812201842433 2117 24-25 -0.0672948960921218 2117 26-27 0.021196691727517702 2117 28-29 -0.399623235064162 2117 30-31 0.19438748087488023 2117 32-33 -0.026841903358779007 2117 34-35 -0.049717306698106256 2117 36-37 -0.28432121536479116 2117 38-39 -0.31524892212900113 2117 40-41 -0.4111728661403973 2117 42-43 0.1125904684421144 2117 44-45 -0.009005259653065423 2117 46-47 0.2662329794679579 2117 48-49 -0.16359185010690425 2117 50-51 0.022167338530408642 2117 52-53 -0.1373438332907 2117 54-55 0.07144232018119112 2117 56-57 -0.24721012642400098 2117 58-59 -0.04332341178013621 2117 60-61 -0.2139921086100074 2117 62-63 -0.12916556731354945 2117 64-65 -0.1994331958152884 2117 66-67 0.09169918671648958 2117 68-69 -0.11950258612013442 2117 70-71 0.010138533383262427 2117 72-73 -0.36568248749256327 2117 74-75 -0.3884283090783356 2117 76 -0.932345768924101 2118 1 0.03452721455070673 2118 2 0.09418693702605196 2118 3 -0.09424264232873014 2118 4 -0.07716717665545048 2118 5 0.040308317426742235 2118 6 0.04357204154022298 2118 7 0.07312486061004364 2118 8 -0.15893216273360622 2118 9 -0.22548984916316783 2118 10-11 0.08557260982792059 2118 12-13 0.11839761913051206 2118 14-15 -0.007168346657699942 2118 16-17 -0.05779246416757644 2118 18-19 -0.10668605025976063 2118 20-21 0.1426613910064134 2118 22-23 0.14146066106857091 2118 24-25 -0.004443128559074694 2118 26-27 -0.16199119165956688 2118 28-29 0.11370045430534503 2118 30-31 0.14131406027157567 2118 32-33 0.04435347386249333 2118 34-35 0.08520728066671523 2118 36-37 0.003175622679854939 2118 38-39 -0.17932041393953568 2118 40-41 -0.035682763060655986 2118 42-43 -0.02680038085909331 2118 44-45 -0.04511083734230681 2118 46-47 0.13441106079344678 2118 48-49 -0.03621643114782103 2118 50-51 -0.01233749926127814 2118 52-53 -0.17861130309402995 2118 54-55 0.16940364946722042 2118 56-57 0.09571578896207456 2118 58-59 0.01813709539749908 2118 60-61 -0.11935478488901907 2118 62-63 -0.10793698956106823 2118 64-65 -0.03836319012377487 2118 66-67 0.08931981531321753 2118 68-69 0.005536938321782259 2118 70-71 -0.0809336856770031 2118 72-73 -0.057735703201160504 2118 74-75 -0.27288255558437413 2118 76 -0.6412087297790876 2119 1 0.03651924642321802 2119 2 0.08223474579099133 2119 3 0.09300835368721039 2119 4 0.0821953731453462 2119 5 0.01839596682913225 2119 6 0.16110192201831097 2119 7 -0.04241298799553306 2119 8 0.12194433129030102 2119 9 -0.058159171872333104 2119 10-11 0.03477579707891465 2119 12-13 0.0028597705249282512 2119 14-15 -0.37270619526327664 2119 16-17 -0.08269286257394981 2119 18-19 -0.030988839104381327 2119 20-21 0.08190441889485811 2119 22-23 -0.031846111839797686 2119 24-25 -0.05324790943556934 2119 26-27 -0.05940155022531002 2119 28-29 0.20732595231331175 2119 30-31 0.059640753498669596 2119 32-33 -0.08612461378690739 2119 34-35 0.08620329660297443 2119 36-37 0.11970948722167662 2119 38-39 -0.05979850158894351 2119 40-41 0.06591086243735589 2119 42-43 -0.07261711392681747 2119 44-45 0.025606294131797824 2119 46-47 0.06568596119185344 2119 48-49 0.12115408678046435 2119 50-51 0.20280194296979914 2119 52-53 -0.07602166165977309 2119 54-55 -0.08458041427780216 2119 56-57 0.12260821924095922 2119 58-59 0.15359526272817448 2119 60-61 0.15156154977233172 2119 62-63 0.07732197458236101 2119 64-65 -0.14095283155803173 2119 66-67 0.13015646869966702 2119 68-69 0.17784769529389166 2119 70-71 -0.07794563786823261 2119 72-73 0.004017284846646874 2119 74-75 0.2739301934196092 2119 76 -0.2766668971097701 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 2.0 20 0.0 21 2.0 22 15.0 23 12.0 24 47.0 25 98.0 26 133.0 27 280.0 28 479.0 29 829.0 30 1348.0 31 2051.0 32 3308.0 33 5708.0 34 9394.0 35 16261.0 36 25711.0 37 128549.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.38110046491991 14.847060398399812 11.216772127459107 33.55506700922117 2 22.010842982695504 18.24823531228923 33.073671528675206 26.66725017634006 3 20.089379952323828 17.47954712784525 30.62138631601168 31.809686603819245 4 15.62759039680374 20.94353514187008 31.479660397370086 31.949214063956095 5 16.000350105804035 29.38159988055214 37.245594072914685 17.372455940729147 6 30.873668439506353 35.70924742697977 16.21041358822409 17.20667054528979 7 29.545840691561935 24.531089910259645 20.18308474105042 25.739984657128 8 25.778084406390462 35.28088267851535 15.477250845659976 23.46378206943422 9 23.497762926884523 15.971517863118928 16.429229715744977 44.101489494251574 10-11 27.12599175191915 24.985197732550056 23.033357875063714 24.85545264046708 12-13 26.213657215526165 18.759492758473336 25.147379097653776 29.87947092834673 14-15 23.086388607145246 18.496913405448264 20.410138652195627 38.00655933521086 16-17 27.950799837303776 24.3774037595185 19.529468096608607 28.14232830656912 18-19 29.280429600416007 23.156152337213673 20.20161975420513 27.361798308165184 20-21 25.212251643695264 21.551586545639896 20.239719503467594 32.99644230719725 22-23 24.194370504615733 25.12240831604257 20.16944091192265 30.51378026741905 24-25 27.84602552683201 25.199122676044013 20.057458540779603 26.897393256344383 26-27 28.080544929386747 27.22304314024312 20.437426310451173 24.25898561991896 28-29 25.353066257523416 25.554891956319153 21.792284285912874 27.29975750024456 30-31 24.352947839383816 25.96240481498453 20.684559819180652 29.000087526451008 32-33 23.02151606110376 29.692576212370064 21.55905203704943 25.726855689476746 34-35 27.524494534745426 23.56392262661731 24.813491430130725 24.09809140850654 36-37 29.27425126269777 22.872978525127817 23.892661679375163 23.96010853279925 38-39 23.906820369979457 23.248312541510707 26.22163756841222 26.62322952009762 40-41 23.408691891446605 19.56731041513281 27.313143898634074 29.710853794786512 42-43 26.750657735536254 19.274611665731335 26.70277561821992 27.27195498051249 44-45 22.273679766458834 23.199658131979593 29.604792330623447 24.92186977093813 46-47 24.773847096438704 20.48324898186143 27.756954491394094 26.985949430305777 48-49 24.209558918173066 19.590221750837937 25.20221184490313 30.99800748608587 50-51 22.391840475320116 20.40267316078609 28.18686382428808 29.018622539605722 52-53 19.219264057005464 20.48299155112317 33.76821966050034 26.529524731371023 54-55 19.517111421172135 21.333800141072047 30.042939447141748 29.106148990614077 56-57 22.254629891827605 21.994882276923395 25.928166526795966 29.822321304453038 58-59 19.997477178765052 22.072368929139614 30.005097128617546 27.925056763477784 60-61 22.804501948750687 24.927275816441586 25.974761490421 26.29346074438672 62-63 19.652777420235086 31.467303721933614 22.115102431690755 26.76481642614055 64-65 19.190946675796877 33.304586900894314 20.58622127716538 26.918245146143434 66-67 19.32197892157115 32.89449973484634 20.019101360778883 27.764419982803627 68-69 19.00945800532367 33.21319898881206 20.26417542360228 27.513167582261993 70-71 19.24140310049581 32.5297203787321 20.410396082933886 27.8184804378382 72-73 19.40847564962647 31.28632991293692 20.087835367894268 29.217359069542344 74-75 19.619053993523043 31.483779289182245 20.295067112193465 28.60209960510125 76 19.807235863191007 31.565642263948885 19.891673145340246 28.735448727519863 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 2.5 16 5.0 17 6.0 18 13.5 19 25.5 20 31.5 21 33.0 22 39.5 23 87.0 24 140.0 25 152.0 26 218.5 27 342.0 28 479.5 29 560.0 30 688.5 31 918.5 32 1339.0 33 1658.0 34 1890.0 35 2422.0 36 2939.0 37 3156.0 38 3813.5 39 7386.0 40 12048.5 41 13161.0 42 12526.0 43 11277.0 44 9700.0 45 9260.5 46 9149.0 47 9177.5 48 8708.0 49 8072.0 50 7934.0 51 7368.0 52 6226.5 53 5360.5 54 5070.0 55 4867.0 56 4463.0 57 4164.5 58 4067.0 59 3982.5 60 3951.5 61 4055.0 62 4105.0 63 4125.0 64 4224.5 65 4385.0 66 4586.5 67 4707.0 68 4640.5 69 4407.0 70 4202.0 71 4164.0 72 3877.5 73 3286.5 74 2735.5 75 2489.0 76 2171.0 77 1458.5 78 822.0 79 580.0 80 423.0 81 174.5 82 57.5 83 32.0 84 18.0 85 3.5 86 2.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.5 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 194227.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.99117012567769 #Duplication Level Percentage of deduplicated Percentage of total 1 60.43600160210563 27.190864297960633 2 22.392859186359214 20.149618745076637 3 8.108943182468387 10.944925267856684 4 3.152714996853007 5.673773471247561 5 1.532299593751788 3.446997585299675 6 0.9555415689191509 2.579455997363909 7 0.6820392515878011 2.1480020800403654 8 0.49093093780397096 1.7670045874157558 9 0.38908279452995365 1.5754761181504116 >10 1.8275447731304 12.940013489370687 >50 0.017165417405733248 0.5452383036344072 >100 0.009154889283057733 0.7717773533030938 >500 0.0011443611603822166 0.3526801114160235 >1k 0.0034330834811466503 4.453551771895771 >5k 0.0 0.0 >10k+ 0.0011443611603822166 5.460620819968388 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT 10606 5.460620819968388 TruSeq Adapter, Index 5 (95% over 23bp) CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 3023 1.5564262435191811 RNA PCR Primer, Index 5 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 2876 1.480741606470779 TruSeq Adapter, Index 5 (95% over 22bp) CACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTG 2751 1.4163839219058112 TruSeq Adapter, Index 5 (96% over 29bp) TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 685 0.3526801114160235 RNA PCR Primer, Index 5 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT 352 0.1812312397349493 TruSeq Adapter, Index 5 (96% over 26bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT 279 0.14364635194900813 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0072080606712763935 0.0 2 0.0 0.0 0.0 0.007722922147796136 0.0 3 0.0 0.0 0.0 0.009782368053875105 0.0 4 0.0 0.0 0.0 0.01956473610775021 0.0 5 0.0 0.0 0.0 0.024713350872947633 0.0010297229530394846 6 0.0 0.0 0.0 0.025228212349467375 0.0010297229530394846 7 0.0 0.0 0.0 0.0267727967790266 0.0010297229530394846 8 0.0 0.0 0.0 0.029347104161625316 0.0010297229530394846 9 0.0 0.0 0.0 0.03295113449726351 0.0010297229530394846 10 0.0 0.0 0.0 0.03758488778594119 0.0010297229530394846 11 0.0 0.0 0.0 0.03809974926246094 0.0010297229530394846 12 0.0 0.0 0.0 0.040159195168539906 0.0010297229530394846 13 0.0 0.0 0.0 0.04170377959809913 0.0010297229530394846 14 0.0 0.0 0.0 0.042218641074618875 0.0010297229530394846 15 0.0 0.0 0.0 0.045822671410257074 0.0010297229530394846 16 0.0 0.0 0.0 0.0473672558398163 0.0010297229530394846 17 0.0 0.0 0.0 0.04839697879285578 0.0010297229530394846 18 0.0 0.0 0.0 0.05251587060501372 0.0010297229530394846 19 0.0 0.0 0.0 0.06126851570584934 0.0010297229530394846 20 0.0 0.0 0.0 0.06950629933016522 0.0010297229530394846 21 0.0 0.0 0.0 0.07362519114232316 0.0010297229530394846 22 0.0 0.0 0.0 0.08083325181359956 0.0010297229530394846 23 0.0 0.0 0.0 0.08649672805531672 0.0010297229530394846 24 0.0 0.0 0.0 0.09318992725007337 0.0010297229530394846 25 0.0 5.148614765197423E-4 0.0 0.10400201825698796 0.0010297229530394846 26 0.0 5.148614765197423E-4 0.0 0.12047758550561971 0.0010297229530394846 27 0.0 5.148614765197423E-4 0.0 0.2126377898026536 0.0010297229530394846 28 0.0 5.148614765197423E-4 0.0 0.33105592940219436 0.0010297229530394846 29 0.0 5.148614765197423E-4 0.0 0.4546226837669325 0.0010297229530394846 30 0.0 5.148614765197423E-4 0.0 0.5792191610847102 0.0010297229530394846 31 0.0 5.148614765197423E-4 0.0 0.7146277294094024 0.0010297229530394846 32 0.0 5.148614765197423E-4 0.0 0.8850468781374371 0.0010297229530394846 33 0.0 5.148614765197423E-4 0.0 1.03899045961684 0.0010297229530394846 34 0.0 5.148614765197423E-4 0.0 1.2995103667358296 0.0010297229530394846 35 0.0 5.148614765197423E-4 0.0 1.6480715863396953 0.0010297229530394846 36 0.0 5.148614765197423E-4 0.0 2.1907355825915036 0.0010297229530394846 37 0.0 5.148614765197423E-4 0.0 2.9563345981763605 0.0010297229530394846 38 0.0 5.148614765197423E-4 0.0 3.7414983498689676 0.0010297229530394846 39 0.0 5.148614765197423E-4 0.0 4.666704423174945 0.0010297229530394846 40 0.0 5.148614765197423E-4 0.0 5.687674731113594 0.0010297229530394846 41 0.0 5.148614765197423E-4 0.0 6.87494529596812 0.0010297229530394846 42 0.0 5.148614765197423E-4 0.0 8.187842061093463 0.0010297229530394846 43 0.0 5.148614765197423E-4 0.0 9.546046636152543 0.0010297229530394846 44 0.0 5.148614765197423E-4 0.0 10.759060274833056 0.0010297229530394846 45 0.0 5.148614765197423E-4 0.0 11.897933860894726 0.0010297229530394846 46 0.0 5.148614765197423E-4 0.0 12.975024069774028 0.0010297229530394846 47 0.0 5.148614765197423E-4 0.0 14.043361633552493 0.0010297229530394846 48 0.0 5.148614765197423E-4 0.0 15.010786347933088 0.0010297229530394846 49 0.0 5.148614765197423E-4 0.0 15.929299222044309 0.0010297229530394846 50 0.0 5.148614765197423E-4 0.0 16.775216627966245 0.0010297229530394846 51 0.0 5.148614765197423E-4 0.0 17.78640456785102 0.0010297229530394846 52 0.0 5.148614765197423E-4 0.0 18.974704855658587 0.0010297229530394846 53 0.0 5.148614765197423E-4 0.0 20.213976429641605 0.0010297229530394846 54 0.0 5.148614765197423E-4 0.0 21.30599762133998 0.0010297229530394846 55 0.0 5.148614765197423E-4 0.0 22.189499915047858 0.0010297229530394846 56 0.0 5.148614765197423E-4 0.0 23.030268706204595 0.0010297229530394846 57 0.0 5.148614765197423E-4 0.0 23.813887873467642 0.0010297229530394846 58 0.0 5.148614765197423E-4 0.0 24.540357416837 0.0010297229530394846 59 0.0 5.148614765197423E-4 0.0 25.266826960206355 0.0010297229530394846 60 0.0 5.148614765197423E-4 0.0 25.991751919146154 0.0010297229530394846 61 0.0 5.148614765197423E-4 0.0 26.673428514058294 0.0010297229530394846 62 0.0 5.148614765197423E-4 0.0 27.414829040246723 0.0010297229530394846 63 0.0 5.148614765197423E-4 0.0 28.222646696906196 0.0010297229530394846 64 0.0 5.148614765197423E-4 0.0 29.09327745370108 0.0010297229530394846 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGATA 15 0.0022221138 70.0 4 GCAACAA 15 0.0022221138 70.0 44 CATAGAT 15 0.0022221138 70.0 34 GAGTTTC 15 0.0022221138 70.0 69 TATGTCG 25 2.3860755E-4 55.999996 36 TCTTTAT 95 0.0 55.263157 1 TCGTTGT 20 0.0069433837 52.5 34 CTGTATA 20 0.0069433837 52.5 9 ACCGATT 20 0.0069433837 52.5 70 AAGACCG 20 0.0069433837 52.5 41 AACGACC 20 0.0069433837 52.5 55 CAATACG 20 0.0069433837 52.5 69 GCAGTAT 30 5.867276E-4 46.666664 42 TTGCGAG 30 5.867276E-4 46.666664 9 CTTTATA 120 0.0 43.75 2 GTTGTAC 35 0.001253194 40.0 8 TTTATAC 150 0.0 37.333332 3 TTGATAT 40 0.0024145024 35.0 22 TGGCGTA 40 0.0024145024 35.0 62 ATAGCAT 40 0.0024145024 35.0 3 >>END_MODULE