FastQCFastQC Report
Wed 25 May 2016
SRR1780283_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780283_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787738
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTA300.005772812829.303121
GCGCTAT703.5393896E-828.324919
ATTACAC655.9803824E-727.0490324
GGCGCTA904.024041E-722.0304818
ACTTTCA602.8447798E-422.0304815
CGAAACT500.002584624821.99269525
CATCGTT703.2344244E-521.98152432
GACTTAC602.8916285E-421.977341
CAAGGAA1206.0572347E-1021.977344
CGCTATC956.765222E-720.87230720
TCGTTTG654.8821475E-420.3371221
AAGGTTC654.884243E-420.33582716
GGTATAC654.9136614E-420.31772838
CGCTATT654.960194E-420.289358
AACGATT708.1658823E-418.84130731
CATCGCA708.169369E-418.840119
GATCCTA708.176346E-418.8377191
GGCTTAT600.00745403918.3144511
CGATTAA750.001297615917.58521833
TAAACCA902.2316357E-417.0934854
Produced by FastQC (version 0.11.5)