FastQCFastQC Report
Wed 25 May 2016
SRR1780283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780283_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787738
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT254513.2308965671327265No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC78050.9908116658076669No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG69170.8780838299028357No Hit
CACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTG44560.5656703117026219TruSeq Adapter, Index 9 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC14740.18711805194112766No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT10390.1318966458390988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAAT250.002353096435.19756315
TCGGAAA250.002353096435.19756314
ATGCCGT35950.034.0838744
CTTTATA2400.033.9186252
TCTTCTG6050.032.36139344
GGCTAAA701.0113581E-931.496371
GTCTTCT6150.031.11979743
TATGCCG41950.029.10406343
TCTTTAT2750.028.8621271
TTAATAC1300.028.7708913
CGTCTTC6800.028.1451142
CCGTCTT7050.027.14705541
GCCGTCT7700.025.14111540
TTTATAC3350.024.9566653
ATAGACC450.001397311924.4458544
TTTAGCG651.8133222E-523.69066626
CGAGAAT651.8133222E-523.69066614
CGATAGA752.063991E-623.46504237
CTTAATA1900.023.1592312
ATATCGT1850.022.59302937
Produced by FastQC (version 0.11.5)