FastQCFastQC Report
Wed 25 May 2016
SRR1780282_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780282_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121188
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT703.783498E-1045.039
GATCTCT350.001251328240.041
GAATGTG400.002410929735.032
CATAGGC400.002410929735.051
TTAACAG400.002410929735.039
CGACGAT552.9777866E-431.8181822
TCGGTGG552.9777866E-431.8181845
GTAGATC901.7367893E-731.11111338
GCCCAAT500.00718533328.00000245
CTGCATG500.00718533328.00000240
GTATCAT657.918084E-426.92307957
AGATCTC959.307598E-625.78947340
TGTAGAT1251.0833355E-725.19999937
GTGTAGA1202.1424185E-623.33333236
ATGCCGA800.002653532421.87518
GGTGTAG1003.9056863E-421.00000235
GACGAAC1003.9056863E-421.00000223
ACCAGAC850.00377151220.58823633
GATGCCG850.00377151220.58823617
ATTGAAC3900.018.84615370