##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780282_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 121188 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.104218239429645 34.0 31.0 34.0 12.0 34.0 2 30.296531009670925 34.0 32.0 34.0 12.0 34.0 3 30.115803544905436 34.0 31.0 34.0 11.0 34.0 4 30.07229263623461 34.0 31.0 34.0 11.0 34.0 5 30.017600752549757 34.0 31.0 34.0 11.0 34.0 6 33.062374162458326 38.0 34.0 38.0 11.0 38.0 7 33.36383140244909 38.0 34.0 38.0 12.0 38.0 8 33.36360860811301 38.0 34.0 38.0 11.0 38.0 9 33.50986071228174 38.0 34.0 38.0 12.0 38.0 10-11 33.27524177311285 38.0 34.0 38.0 11.0 38.0 12-13 33.34864838102782 38.0 34.0 38.0 11.0 38.0 14-15 33.23545648083969 38.0 34.0 38.0 11.0 38.0 16-17 33.28809370564743 38.0 34.0 38.0 11.0 38.0 18-19 33.31360778294881 38.0 34.0 38.0 11.0 38.0 20-21 33.319829521074695 38.0 34.0 38.0 11.0 38.0 22-23 33.33412136515167 38.0 34.0 38.0 11.0 38.0 24-25 33.32782536224708 38.0 34.0 38.0 11.0 38.0 26-27 33.297422187015215 38.0 34.0 38.0 11.0 38.0 28-29 33.34418424266429 38.0 34.0 38.0 11.0 38.0 30-31 33.39661105059907 38.0 34.0 38.0 11.0 38.0 32-33 33.43828184308677 38.0 34.0 38.0 11.0 38.0 34-35 33.38839654091164 38.0 34.0 38.0 11.0 38.0 36-37 33.34425850744298 38.0 34.0 38.0 11.0 38.0 38-39 33.338474106347164 38.0 34.0 38.0 11.0 38.0 40-41 33.40449962042447 38.0 34.0 38.0 11.0 38.0 42-43 33.46711720632406 38.0 34.0 38.0 11.0 38.0 44-45 33.53221441066772 38.0 34.0 38.0 11.0 38.0 46-47 33.42816945572169 38.0 34.0 38.0 11.0 38.0 48-49 33.39789830676305 38.0 34.0 38.0 11.0 38.0 50-51 33.350612271842095 38.0 34.0 38.0 11.0 38.0 52-53 33.391532164900816 38.0 34.0 38.0 11.0 38.0 54-55 33.39295144733802 38.0 34.0 38.0 11.0 38.0 56-57 33.34026883849886 38.0 34.0 38.0 11.0 38.0 58-59 33.3640046869327 38.0 34.0 38.0 11.0 38.0 60-61 33.37358484338384 38.0 34.0 38.0 11.0 38.0 62-63 33.25477770076245 38.0 34.0 38.0 11.0 38.0 64-65 33.2418102452388 38.0 34.0 38.0 11.0 38.0 66-67 33.149305211737136 38.0 34.0 38.0 11.0 38.0 68-69 33.08207083209558 38.0 34.0 38.0 11.0 38.0 70-71 33.08446793411889 38.0 34.0 38.0 11.0 38.0 72-73 33.021004554906426 38.0 34.0 38.0 11.0 38.0 74-75 33.01769564643364 38.0 34.0 38.0 11.0 38.0 76 31.52764300095719 37.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5073554444634638 1101 2 -0.07967668792205629 1101 3 -0.3769322971856255 1101 4 0.059168608769397224 1101 5 -0.5810520222627211 1101 6 -0.7067175248659652 1101 7 -0.5314704351687709 1101 8 -0.22577955387185966 1101 9 -0.3295471292781258 1101 10-11 -0.44282581589978065 1101 12-13 -0.6135838494483323 1101 14-15 -0.8127355800033556 1101 16-17 -0.6928472405968478 1101 18-19 -0.4357015538627067 1101 20-21 -0.1794859384287335 1101 22-23 -0.8211880133783893 1101 24-25 -0.4912733838912686 1101 26-27 -0.45147461000905054 1101 28-29 -0.40245500888872954 1101 30-31 -0.49531508328930585 1101 32-33 -0.8260413459789717 1101 34-35 -0.6197666258863492 1101 36-37 -0.31925292557583873 1101 38-39 -0.3419485635054329 1101 40-41 -0.48706917442726194 1101 42-43 -0.8286868102501543 1101 44-45 -0.5102586782129848 1101 46-47 -0.5356177335332788 1101 48-49 -0.33995800667604925 1101 50-51 -0.46580554507946204 1101 52-53 -0.7664310231183862 1101 54-55 -0.40867825170523275 1101 56-57 -0.4455545305441575 1101 58-59 -0.5716747689405537 1101 60-61 -0.7792126366134156 1101 62-63 -0.6699742666245854 1101 64-65 -0.8381547894385051 1101 66-67 -1.0581913340318678 1101 68-69 -0.9283433826105281 1101 70-71 -0.6448946556091073 1101 72-73 -0.47660707696019244 1101 74-75 -0.7851285397275589 1101 76 -1.5108048819148614 1102 1 -0.36948527981057566 1102 2 -0.18667570385980525 1102 3 0.5567601681439349 1102 4 -0.24836606167119868 1102 5 -0.24890565061182812 1102 6 -0.32835066240134125 1102 7 -0.3538827065264698 1102 8 -0.294653766368441 1102 9 0.08290683791303621 1102 10-11 -0.30346434041510406 1102 12-13 -0.42531853814988096 1102 14-15 -0.1978539210975967 1102 16-17 -0.17293773374392174 1102 18-19 -0.657952520171257 1102 20-21 -0.13495458539386362 1102 22-23 -0.4649371890994374 1102 24-25 -0.326635711553557 1102 26-27 -0.4639529251321619 1102 28-29 -0.7460195461258436 1102 30-31 -0.0138651639418228 1102 32-33 -0.6550072690192152 1102 34-35 -0.5961339144397755 1102 36-37 -0.5284828207782866 1102 38-39 -0.24330900420300594 1102 40-41 -0.7268252893089198 1102 42-43 -0.09940537859176146 1102 44-45 -0.3452354969527818 1102 46-47 -0.6973539439805805 1102 48-49 -0.8716711880377161 1102 50-51 -0.6089916748742326 1102 52-53 -0.31686167716470237 1102 54-55 -0.5493890084186717 1102 56-57 -0.7041650752276851 1102 58-59 -0.44525827727464673 1102 60-61 -0.5065513062018354 1102 62-63 -0.27485298348857157 1102 64-65 -0.3886158783997615 1102 66-67 -0.48883523538216167 1102 68-69 -0.1520958047225065 1102 70-71 -0.4495227916734663 1102 72-73 -0.26835617152147506 1102 74-75 -0.702137599187246 1102 76 -0.72367682193779 1103 1 -0.5966389641881804 1103 2 -0.1391478279818621 1103 3 -0.2535350582359719 1103 4 -0.05941522638688568 1103 5 -0.4824182096372631 1103 6 -0.562131067940804 1103 7 -0.35059910666932126 1103 8 -0.24691756542403454 1103 9 -0.6482149429686714 1103 10-11 -0.143713735280393 1103 12-13 0.11834540375718916 1103 14-15 -0.8888334457196336 1103 16-17 -0.21596182008969578 1103 18-19 -0.3467099107509952 1103 20-21 -0.174442105730364 1103 22-23 -0.29191330115848757 1103 24-25 -0.2896480920146516 1103 26-27 -0.21978602699319794 1103 28-29 -0.10994855687938099 1103 30-31 -0.17598670673774564 1103 32-33 -0.15201631627923717 1103 34-35 -0.07094642187995959 1103 36-37 0.1205539237953488 1103 38-39 -0.3573719382135536 1103 40-41 -0.29130189738003054 1103 42-43 -0.219355975790279 1103 44-45 -0.0010824871515850987 1103 46-47 -0.12538259363733317 1103 48-49 0.11959403953262182 1103 50-51 -0.5125570095831691 1103 52-53 0.1830589609052069 1103 54-55 0.22131949521213556 1103 56-57 0.21647795481080578 1103 58-59 -0.31398868479816144 1103 60-61 -0.14808834838270712 1103 62-63 -0.10990913205409925 1103 64-65 -0.11685086907473163 1103 66-67 -0.35395963520756624 1103 68-69 0.16559992163755766 1103 70-71 0.06784755077325855 1103 72-73 0.17767275590203724 1103 74-75 0.1338014508504557 1103 76 0.39225111742969787 1104 1 -0.0307473430936831 1104 2 -0.008655266124812044 1104 3 -0.12235413353521807 1104 4 0.14186398123099764 1104 5 -0.11851328019445617 1104 6 0.138411973858112 1104 7 -0.12775119661539946 1104 8 -0.8273986545670198 1104 9 -0.6477942767864135 1104 10-11 3.883336323795561E-4 1104 12-13 -0.49538265467717224 1104 14-15 -0.22233090260134958 1104 16-17 -0.430090467456715 1104 18-19 -0.21357384436222304 1104 20-21 -0.5551593415711196 1104 22-23 -0.18307070679540516 1104 24-25 -0.22316849432449715 1104 26-27 -0.3057262159105534 1104 28-29 -0.6236441092905736 1104 30-31 -0.272410088129007 1104 32-33 -0.24497876226187998 1104 34-35 0.03728907469734821 1104 36-37 -0.7260128665216783 1104 38-39 -0.12646444653127276 1104 40-41 -0.5485822905117033 1104 42-43 -0.028053249108573652 1104 44-45 -0.09299804797608857 1104 46-47 0.1275047060682013 1104 48-49 -0.03922178516430819 1104 50-51 -0.39869988311561855 1104 52-53 -0.14737432526252547 1104 54-55 0.015035316084826889 1104 56-57 -0.1639620237734647 1104 58-59 -0.3015264491486107 1104 60-61 -0.0793524502604086 1104 62-63 -0.1826748209905773 1104 64-65 0.005610792939108933 1104 66-67 -0.015973478949199205 1104 68-69 -0.1840214787984067 1104 70-71 -0.05966210542774064 1104 72-73 0.020826413785712816 1104 74-75 -0.2070528839233461 1104 76 0.28377660597610443 1105 1 0.5616705498992047 1105 2 -0.02842916730403644 1105 3 -0.06311039510816485 1105 4 0.26816746042405626 1105 5 0.44344949146522694 1105 6 1.1696370006586179 1105 7 0.4561587536818408 1105 8 -0.15512499702877136 1105 9 0.5825731337973536 1105 10-11 0.88667621980332 1105 12-13 0.2324021616175429 1105 14-15 0.5374732100582307 1105 16-17 0.6664595763986583 1105 18-19 0.4583635886365087 1105 20-21 0.2152577717706592 1105 22-23 0.43998747466838495 1105 24-25 0.22034578303638597 1105 26-27 0.4698219762734439 1105 28-29 0.2894491564730046 1105 30-31 0.29519540841129555 1105 32-33 -0.1748930006402034 1105 34-35 0.32605235313025105 1105 36-37 0.1563134174662295 1105 38-39 0.20304047501632994 1105 40-41 0.16632366407362298 1105 42-43 0.31926182739469056 1105 44-45 0.23623886850378284 1105 46-47 0.24370683061746234 1105 48-49 0.37258505627415417 1105 50-51 0.7891375596287489 1105 52-53 0.5441859515136187 1105 54-55 0.5558744155878017 1105 56-57 0.18864006179324377 1105 58-59 0.027140347459905456 1105 60-61 0.2686838035160335 1105 62-63 0.3263301492852193 1105 64-65 0.28923324689097285 1105 66-67 0.39942950321627535 1105 68-69 0.22266402943666463 1105 70-71 0.17133877167974987 1105 72-73 0.12627422588979442 1105 74-75 -0.03933043971712635 1105 76 0.372773779740843 1106 1 0.5791763282129416 1106 2 0.9534721491465703 1106 3 0.4872965991637699 1106 4 0.932924771247091 1106 5 0.5408969841795255 1106 6 0.9756464469454258 1106 7 0.2722073920096477 1106 8 0.8609336905403211 1106 9 0.3872359817523332 1106 10-11 0.4676027598495409 1106 12-13 0.7775935996639731 1106 14-15 1.1242634823926707 1106 16-17 1.58011649530129 1106 18-19 1.198012870115754 1106 20-21 1.0571078370907259 1106 22-23 0.946951598877007 1106 24-25 0.8879269306597379 1106 26-27 0.675389255943827 1106 28-29 0.8697677174216523 1106 30-31 0.6486071258266293 1106 32-33 0.6981700081026361 1106 34-35 0.40498914340258096 1106 36-37 0.7729800841329038 1106 38-39 0.8492016648464968 1106 40-41 0.6052092032154164 1106 42-43 0.4746431961013613 1106 44-45 0.37594342081008847 1106 46-47 0.6110719195532468 1106 48-49 0.9564911963605738 1106 50-51 0.6724759175827231 1106 52-53 0.8441457545484994 1106 54-55 0.8347358365505215 1106 56-57 0.6282893432724919 1106 58-59 0.8678648977563554 1106 60-61 0.40357476924811664 1106 62-63 0.42271242242360074 1106 64-65 0.683289120672498 1106 66-67 0.3416513904637881 1106 68-69 0.9992191495250964 1106 70-71 0.9466578350904697 1106 72-73 1.1408958719555216 1106 74-75 0.3935908747236354 1106 76 0.5076144992665235 1107 1 0.5709535916627786 1107 2 0.267869750685648 1107 3 0.20879778569879548 1107 4 -0.022660732517593374 1107 5 0.07397836192735596 1107 6 -0.44747291945463274 1107 7 -0.009055120319573007 1107 8 0.3855970173306247 1107 9 0.3932516654992426 1107 10-11 0.28901214861311075 1107 12-13 0.37875669695984016 1107 14-15 0.46829027952936286 1107 16-17 0.6671876581056111 1107 18-19 0.6978468528694961 1107 20-21 0.8948259788694557 1107 22-23 0.5256584221858276 1107 24-25 0.6937404639950273 1107 26-27 0.9012508366233263 1107 28-29 0.33078296171114374 1107 30-31 0.359175588409272 1107 32-33 0.6145852074698155 1107 34-35 0.5120165557796597 1107 36-37 0.6972225083753258 1107 38-39 0.29901074501329816 1107 40-41 0.5270932059888835 1107 42-43 0.047930044605735134 1107 44-45 0.6187143463074065 1107 46-47 0.27600028799434284 1107 48-49 0.15497457998736763 1107 50-51 0.30009923421668105 1107 52-53 0.259940830672285 1107 54-55 0.5822724335875975 1107 56-57 0.6296907241453553 1107 58-59 0.3650865503115597 1107 60-61 0.2990913270269999 1107 62-63 0.4116673926869865 1107 64-65 0.5377164100014937 1107 66-67 0.790161629821597 1107 68-69 0.5453963582869008 1107 70-71 0.33574366600678474 1107 72-73 0.516910286158776 1107 74-75 0.587831052850369 1107 76 0.947169573046537 1108 1 -0.3747292908017137 1108 2 -0.4799823333733322 1108 3 -0.48411796181465405 1108 4 -0.3271133042115473 1108 5 -0.001014865364723505 1108 6 0.22553106676774348 1108 7 0.2539484788909263 1108 8 0.24812072180834832 1108 9 0.12526528831746475 1108 10-11 0.02224969650582409 1108 12-13 -0.4983525177930517 1108 14-15 -0.5828872041193804 1108 16-17 -0.375635206556332 1108 18-19 -0.2539024408338193 1108 20-21 -0.29813330748063294 1108 22-23 -0.5296262250528372 1108 24-25 -0.3862452165653636 1108 26-27 -0.8973723686090764 1108 28-29 -0.3041390553947778 1108 30-31 0.16941882537664554 1108 32-33 -0.07190614281463326 1108 34-35 0.0640551408642196 1108 36-37 -0.7177606566078438 1108 38-39 0.2003323905878176 1108 40-41 -0.4306596095225501 1108 42-43 0.0832303658255924 1108 44-45 -0.21484635650616468 1108 46-47 -0.35241025420929617 1108 48-49 -0.32969491186603506 1108 50-51 -0.16183814726093004 1108 52-53 -0.2668355538627196 1108 54-55 -0.7013637365428238 1108 56-57 -0.26652720224498694 1108 58-59 -0.23219856134137729 1108 60-61 -0.11341496191607092 1108 62-63 -0.598012741993152 1108 64-65 -0.4080487124877692 1108 66-67 -0.010546601921113563 1108 68-69 0.027472870535824256 1108 70-71 0.004751754555101684 1108 72-73 -0.5000698601317097 1108 74-75 -0.424137289577736 1108 76 -0.2773456945721797 1109 1 0.92195280796372 1109 2 0.5289297036637066 1109 3 0.6203573468273191 1109 4 0.21756262171437868 1109 5 0.6362922334007095 1109 6 1.1569353877553965 1109 7 0.3617031085205511 1109 8 0.35525806748736244 1109 9 0.5777344241199316 1109 10-11 0.8981177520613812 1109 12-13 0.3061420809723785 1109 14-15 1.1035518390904926 1109 16-17 0.6328910280115636 1109 18-19 0.7635551208945728 1109 20-21 0.3941892233835631 1109 22-23 0.5307132811200006 1109 24-25 0.016918363681547532 1109 26-27 0.6520296375637713 1109 28-29 0.4940862532471968 1109 30-31 0.8957498438951674 1109 32-33 0.5736957090372172 1109 34-35 1.2513043692592802 1109 36-37 0.3610009174662352 1109 38-39 0.9671533782421378 1109 40-41 0.6037732608478166 1109 42-43 0.3197658596527546 1109 44-45 0.6117933039876604 1109 46-47 0.8174971531981114 1109 48-49 0.7718290078870567 1109 50-51 0.8540569144674564 1109 52-53 0.7935811128039418 1109 54-55 0.8276486091361903 1109 56-57 0.6703940940513107 1109 58-59 0.8438238152018442 1109 60-61 0.8811838035160307 1109 62-63 0.7406446654142584 1109 64-65 0.9130739726974184 1109 66-67 1.0167682128936946 1109 68-69 1.0777043520172995 1109 70-71 1.1265303039378196 1109 72-73 0.8665968065349574 1109 74-75 1.1966574635086786 1109 76 0.31733023135374694 1110 1 -0.17391257665166293 1110 2 0.17013162674063054 1110 3 0.1878573468273217 1110 4 -0.2588796859779272 1110 5 -0.12404430506082775 1110 6 -0.31681461224460605 1110 7 0.04588580082824478 1110 8 0.12020999056428394 1110 9 0.2092728856583932 1110 10-11 -0.25104090178477634 1110 12-13 -0.17022811133531235 1110 14-15 -0.1387798916787375 1110 16-17 0.23380448955003175 1110 18-19 -0.17704584064389195 1110 20-21 0.07106422338355856 1110 22-23 0.20936712727384332 1110 24-25 0.1798991329123183 1110 26-27 -0.16030209320546618 1110 28-29 -0.047548362137412425 1110 30-31 -0.08937034841252256 1110 32-33 -0.05774659865509335 1110 34-35 -0.5512677461253332 1110 36-37 0.15862110977392518 1110 38-39 -0.4538081602193955 1110 40-41 -0.17528923915218542 1110 42-43 -0.13845529419339897 1110 44-45 -0.16063458062772895 1110 46-47 -0.3142336160326593 1110 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-0.6580211424700977 2116 22-23 -0.10373793839220014 2116 24-25 -0.2368926119282051 2116 26-27 -0.20014261853379622 2116 28-29 -0.4624686248015806 2116 30-31 -0.49194070488532304 2116 32-33 -0.4837738031579093 2116 34-35 -0.6033831307407169 2116 36-37 -0.49277194838742133 2116 38-39 -0.23985881687980992 2116 40-41 -0.3041916781765721 2116 42-43 -0.307596945225292 2116 44-45 -0.09906797650014454 2116 46-47 0.042802031246885974 2116 48-49 -0.20618166284465644 2116 50-51 -0.09807723187400086 2116 52-53 0.07208721036491994 2116 54-55 -0.11229041525405847 2116 56-57 -0.09827968643649143 2116 58-59 -0.05179356284693881 2116 60-61 0.0671594132721296 2116 62-63 -0.08084923702477198 2116 64-65 -0.40731474681477664 2116 66-67 -0.33292690905752664 2116 68-69 -0.3599480870070835 2116 70-71 -0.26954439118413376 2116 72-73 -0.13523246175773096 2116 74-75 -0.1349736340522938 2116 76 -0.07398074425600853 2117 1 -0.13124076570399268 2117 2 -0.14905814900087222 2117 3 0.34045922770506465 2117 4 -0.4395886322041136 2117 5 0.03009121459193409 2117 6 0.23277786424442581 2117 7 0.3169636884578537 2117 8 0.23512679789488544 2117 9 -0.13339410252583406 2117 10-11 0.30468612980432397 2117 12-13 0.013552935204351968 2117 14-15 0.15010846134754985 2117 16-17 0.0010434574786515327 2117 18-19 0.155527103653192 2117 20-21 -0.48761916219637413 2117 22-23 -0.1843729257765574 2117 24-25 0.23135801885396035 2117 26-27 0.37773986483649935 2117 28-29 0.058055688983877474 2117 30-31 0.01748279844061784 2117 32-33 -0.053126391276265394 2117 34-35 0.2675024491965843 2117 36-37 0.13295918549130903 2117 38-39 -0.20882624558230845 2117 40-41 -0.0011199601553215643 2117 42-43 -0.03043986135038068 2117 44-45 -0.019216688175355046 2117 46-47 0.2383827331354169 2117 48-49 -0.16634203443269513 2117 50-51 0.071259108824826 2117 52-53 -0.15385227277599256 2117 54-55 0.08070503546847618 2117 56-57 -0.3013437586132639 2117 58-59 -0.039302164421982866 2117 60-61 -0.3936986416250363 2117 62-63 -0.3124705383475046 2117 64-65 -0.6801519285564979 2117 66-67 -0.2944778686110041 2117 68-69 -0.490653798452918 2117 70-71 -0.17052592646970766 2117 72-73 -1.3953154191703732 2117 74-75 -0.5595043703471205 2117 76 -0.7895075429409246 2118 1 -0.28854247505514863 2118 2 -0.29986353533000454 2118 3 -0.45377787329846697 2118 4 -0.25181630910322994 2118 5 -0.6019349678571508 2118 6 -0.31983348016913027 2118 7 -0.5802583694668684 2118 8 -0.008401523707604497 2118 9 -0.7232089721064838 2118 10-11 -1.1258111001398774 2118 12-13 -0.49628519890183753 2118 14-15 -0.7118471388969283 2118 16-17 -0.8816333430576222 2118 18-19 -0.5034948004890722 2118 20-21 -0.5039043300755566 2118 22-23 -0.5061577880623958 2118 24-25 -0.29184735958889263 2118 26-27 -0.8904133655808835 2118 28-29 -0.6495851304006024 2118 30-31 -0.5628488196268435 2118 32-33 -0.9637649827866852 2118 34-35 -0.7728013697344309 2118 36-37 -0.667271488194146 2118 38-39 -1.294717690940253 2118 40-41 -0.7481823209131875 2118 42-43 -0.6957412158189413 2118 44-45 -0.4749500136224043 2118 46-47 -0.8994053625251652 2118 48-49 -0.40915566192426667 2118 50-51 -0.5891034628910248 2118 52-53 -0.8588166859381943 2118 54-55 -0.6489237179078344 2118 56-57 -0.683281220414095 2118 58-59 -0.7298691879428176 2118 60-61 -0.6593036178676144 2118 62-63 -0.8942216867870059 2118 64-65 -0.8651591247868566 2118 66-67 -0.7900714097478172 2118 68-69 -0.8330267800581694 2118 70-71 -0.8436642715338749 2118 72-73 -0.8727113695656783 2118 74-75 -1.1285980396359712 2118 76 -0.6360109636148046 2119 1 -0.6171836273675098 2119 2 -0.8711787348220987 2119 3 -1.3413540096395558 2119 4 -0.3026069366452404 2119 5 0.19738253308525344 2119 6 -0.4090543598786738 2119 7 -0.1487562763374939 2119 8 -0.4742864908722595 2119 9 -0.8088586358169749 2119 10-11 -0.6378887542477685 2119 12-13 -0.7490876114566447 2119 14-15 -0.5161494621713345 2119 16-17 -0.5806046344489957 2119 18-19 -0.581501069957163 2119 20-21 -0.5155603822946659 2119 22-23 -0.9908561195109158 2119 24-25 -0.11803826092413061 2119 26-27 -0.4804476889346603 2119 28-29 -0.3616905606329297 2119 30-31 -0.733592624559094 2119 32-33 -0.8123255843381756 2119 34-35 -0.6303941717501758 2119 36-37 -0.7312260147104226 2119 38-39 -1.0798892085086536 2119 40-41 -0.507844444199506 2119 42-43 -0.9819592980759495 2119 44-45 -0.3152620982205505 2119 46-47 -0.6516274524801275 2119 48-49 -0.7749523406933818 2119 50-51 -0.5280803095072955 2119 52-53 -0.39945910801624507 2119 54-55 -0.48725675363983356 2119 56-57 -0.6622656062641497 2119 58-59 -0.7055501989937483 2119 60-61 -0.7909376475880734 2119 62-63 -1.1405264702324374 2119 64-65 -0.9787783538010046 2119 66-67 -0.8092964558760158 2119 68-69 -1.265887919276068 2119 70-71 -1.0825637418035043 2119 72-73 -0.4824568212252984 2119 74-75 -0.6933957699298006 2119 76 0.07681366983955229 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 26.0 13 306.0 14 1084.0 15 1889.0 16 2507.0 17 2743.0 18 2833.0 19 2550.0 20 1953.0 21 1540.0 22 1170.0 23 906.0 24 803.0 25 837.0 26 884.0 27 1100.0 28 1269.0 29 1575.0 30 1815.0 31 2357.0 32 2829.0 33 3905.0 34 5247.0 35 8070.0 36 15300.0 37 55690.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.289665643463046 16.99590718552992 12.75621348648381 27.95821368452322 2 24.251576063636666 19.14793543915239 28.963263689474207 27.637224807736736 3 19.948344720599398 21.907284549625373 26.953988843779914 31.19038188599531 4 15.777139650790506 17.461299798659933 29.00452189985807 37.757038650691484 5 17.728653001947386 29.80080536026669 30.338812423672312 22.13172921411361 6 29.861867511634816 32.055153975641154 17.528138099481797 20.554840413242236 7 29.652275802884777 27.637224807736736 18.04304056507245 24.66745882430604 8 25.722018681717664 31.772947816615506 17.669241178994618 24.835792322672212 9 24.372875202165233 18.79311482985114 18.344225500874675 38.489784467108954 10-11 26.690348879427006 22.59093309568604 23.58319305541803 27.13552496946892 12-13 25.792570221474076 20.141020563092056 23.671898207743343 30.39451100769053 14-15 24.836617486879888 21.262006139221707 21.66221077994521 32.239165593953196 16-17 25.909743538964253 23.112024292834274 22.145757005644125 28.832475162557348 18-19 24.57627817935769 22.563290094728853 23.413209228636497 29.44722249727696 20-21 24.70046539261313 22.71924612997987 23.69128956662376 28.888998910783247 22-23 26.013714229131597 22.415998283658446 22.574017229428655 28.996270257781298 24-25 25.19350100670033 23.350496748853022 22.157721886655445 29.2982803577912 26-27 24.344819619104204 23.837343631382645 23.5114037693501 28.306432980163056 28-29 24.926972967620557 22.91563521140707 24.615060897118525 27.542330923853847 30-31 24.710367363105256 22.45808165824999 24.57792850777305 28.253622470871704 32-33 24.00031356239892 23.448278707462787 24.498300161732185 28.053107568406112 34-35 24.405056606264647 23.044773409908572 24.418259233587484 28.131910750239296 36-37 24.580816582499917 22.203105918077696 24.75698914083903 28.459088358583358 38-39 23.58195530910651 22.243126382150045 26.326864045945143 27.848054262798293 40-41 23.605885071129155 21.620539987457505 26.498910783245865 28.27466415816747 42-43 22.599597319866653 21.42043766709575 26.343779912202532 29.636185100835068 44-45 21.762880813281843 21.075931610390466 28.101792256659074 29.059395319668614 46-47 22.097484899494997 21.795062217381258 27.875284681651646 28.232168201472092 48-49 21.438591279664653 22.06942931643397 27.914067399412485 28.577912004488894 50-51 21.06644222200218 23.513054097765455 26.785242763309896 28.635260916922466 52-53 20.56556754794204 23.494900485196553 26.606182130243916 29.33334983661749 54-55 20.542050368023236 22.75555335511767 26.82815130210912 29.874244974749974 56-57 20.57423177212265 23.235386341882034 26.510463082153347 29.679918803841964 58-59 20.916262336204905 23.09180776974618 25.91180644948345 30.08012344456547 60-61 20.737614285242763 22.03807307654223 26.715516387761163 30.50879625045384 62-63 20.987226458065155 22.68376406904974 26.039294319569596 30.289715153315512 64-65 21.397745651384625 22.843845925339142 25.075089942898636 30.683318480377597 66-67 21.68655312407169 23.43713899065914 23.77050533056078 31.10580255470839 68-69 21.34080932105489 24.044459847509657 23.715219328646402 30.899511502789057 70-71 21.536785820378253 24.380714262138163 24.1665841502459 29.91591576723768 72-73 21.791348978446713 23.90665742482754 25.14770439317424 29.15428920355151 74-75 21.533485163547546 25.56317457173978 24.527593491104728 28.375746773607947 76 20.595273459418426 25.206291051919333 23.9809222035185 30.217513285143742 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 1.5 12 1.0 13 0.5 14 1.5 15 6.0 16 8.5 17 8.0 18 9.5 19 19.0 20 34.5 21 42.0 22 57.0 23 95.5 24 127.5 25 136.0 26 199.5 27 290.5 28 421.5 29 525.0 30 593.5 31 798.5 32 1084.5 33 1234.0 34 1433.0 35 1894.0 36 2339.0 37 2522.0 38 2840.0 39 3584.5 40 4344.0 41 4906.0 42 5135.0 43 5329.0 44 5823.5 45 6186.0 46 6248.0 47 6270.0 48 6077.5 49 5623.5 50 5384.0 51 5068.0 52 4504.0 53 3882.0 54 3508.0 55 3367.0 56 3035.0 57 2873.0 58 2902.0 59 2737.0 60 2556.0 61 2577.0 62 2614.0 63 2563.0 64 2536.5 65 2543.0 66 2614.0 67 2703.0 68 2704.5 69 2720.5 70 2692.5 71 2650.0 72 2513.5 73 2321.0 74 2081.0 75 1897.0 76 1686.5 77 1281.0 78 869.0 79 652.0 80 511.0 81 271.5 82 121.0 83 69.0 84 51.0 85 23.5 86 11.5 87 9.0 88 8.0 89 6.5 90 4.5 91 5.5 92 8.0 93 6.5 94 5.0 95 5.0 96 5.0 97 5.5 98 5.5 99 12.0 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 121188.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.0884245964947 #Duplication Level Percentage of deduplicated Percentage of total 1 76.95570502332909 52.39792718751032 2 13.72356541234927 18.688318975476122 3 4.642792219596437 9.483612238835528 4 1.813003696297643 4.93778261874113 5 0.9501302793431498 3.2346436940951246 6 0.5877719202569229 2.4012278443410238 7 0.3841725746833909 1.8310393768359905 8 0.2593467854329516 1.412681123543585 9 0.1914803369084409 1.17338350331716 >10 0.49203175180270253 4.4393834373040235 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.003300656830709311 0.0 2 0.0 0.0 0.0 0.003300656830709311 0.0 3 0.0 0.0 0.0 0.004125821038386639 0.0 4 0.0 0.0 0.0 0.010727134699805262 0.0 5 0.0 0.0 0.0 0.014027791530514573 0.0 6 0.0 0.0 0.0 0.014852955738191901 0.0 7 0.0 0.0 0.0 0.014852955738191901 0.0 8 0.0 0.0 0.0 0.015678119945869227 0.0 9 0.0 0.0 0.0 0.01815361256890121 0.0 10 0.0 0.0 0.0 0.024754926230319833 0.0 11 0.0 0.0 0.0 0.028880747268706472 0.0 12 0.0 0.0 0.0 0.028880747268706472 0.0 13 0.0 0.0 0.0 0.033006568307093115 0.0 14 0.0 0.0 0.0 0.03383173251477044 0.0 15 0.0 0.0 0.0 0.03465689672244777 0.0 16 0.0 0.0 0.0 0.03630722513780242 0.0 17 0.0 0.0 0.0 0.038782717760834404 0.0 18 0.0 0.0 0.0 0.04538403142225303 0.0 19 0.0 0.0 0.0 0.051160180875994325 0.0 20 0.0 0.0 0.0 0.057761494537412944 0.0 21 0.0 0.0 0.0 0.058586658745090274 0.0 22 0.0 0.0 0.0 0.06931379344489554 0.0 23 0.0 0.0 0.0 0.07343961448328218 0.0 24 0.0 0.0 0.0 0.07839059972934614 0.0 25 0.0 0.0 0.0 0.08664224180611942 0.0 26 0.0 0.0 0.0 0.10809651120572994 0.0 27 0.0 0.0 0.0 0.19638908142720402 0.0 28 0.0 0.0 0.0 0.2846816516486781 0.0 29 0.0 0.0 0.0 0.40350529755421327 0.0 30 0.0 0.0 0.0 0.5371818991979403 0.0 31 0.0 0.0 0.0 0.6692081724263128 0.0 32 0.0 0.0 0.0 0.8012344456546853 0.0 33 0.0 0.0 0.0 0.9984486912895666 8.251642076773278E-4 34 0.0 0.0 0.0 1.2327953262699276 8.251642076773278E-4 35 0.0 0.0 0.0 1.576063636663696 8.251642076773278E-4 36 0.0 0.0 0.0 2.0678615044393833 8.251642076773278E-4 37 0.0 0.0 0.0 2.7329438558273096 8.251642076773278E-4 38 0.0 0.0 0.0 3.3988513714229134 8.251642076773278E-4 39 0.0 0.0 0.0 4.185232861339407 8.251642076773278E-4 40 0.0 0.0 0.0 4.936132290325775 8.251642076773278E-4 41 0.0 0.0 0.0 5.972538535168499 8.251642076773278E-4 42 0.0 0.0 0.0 7.03452487044922 8.251642076773278E-4 43 0.0 0.0 0.0 8.160048849721095 8.251642076773278E-4 44 0.0 0.0 0.0 9.199755751394527 8.251642076773278E-4 45 0.0 0.0 0.0 10.154470739677196 8.251642076773278E-4 46 0.0 0.0 0.0 11.078654652275803 8.251642076773278E-4 47 0.0 0.0 0.0 11.96900683235964 8.251642076773278E-4 48 0.0 0.0 0.0 12.816450473644256 8.251642076773278E-4 49 0.0 0.0 0.0 13.556622767930818 8.251642076773278E-4 50 0.0 0.0 0.0 14.248935538172097 8.251642076773278E-4 51 0.0 0.0 0.0 15.030366042842525 8.251642076773278E-4 52 0.0 0.0 0.0 15.907515595603526 8.251642076773278E-4 53 0.0 0.0 0.0 16.883684853285803 8.251642076773278E-4 54 0.0 0.0 0.0 17.817770736376538 8.251642076773278E-4 55 0.0 0.0 0.0 18.667689870284185 8.251642076773278E-4 56 0.0 0.0 0.0 19.470574644354226 8.251642076773278E-4 57 0.0 0.0 0.0 20.185166848202794 8.251642076773278E-4 58 0.0 0.0 0.0 20.83787173647556 8.251642076773278E-4 59 0.0 0.0 0.0 21.44601775753375 8.251642076773278E-4 60 0.0 0.0 0.0 22.02363270290788 8.251642076773278E-4 61 0.0 0.0 0.0 22.643331022873554 8.251642076773278E-4 62 0.0 0.0 0.0 23.308413374261477 8.251642076773278E-4 63 0.0 0.0 0.0 23.997425487672047 8.251642076773278E-4 64 0.0 0.0 0.0 24.74419909562003 8.251642076773278E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGATCT 70 3.783498E-10 45.0 39 GATCTCT 35 0.0012513282 40.0 41 GAATGTG 40 0.0024109297 35.0 32 CATAGGC 40 0.0024109297 35.0 51 TTAACAG 40 0.0024109297 35.0 39 CGACGAT 55 2.9777866E-4 31.81818 22 TCGGTGG 55 2.9777866E-4 31.81818 45 GTAGATC 90 1.7367893E-7 31.111113 38 GCCCAAT 50 0.007185333 28.000002 45 CTGCATG 50 0.007185333 28.000002 40 GTATCAT 65 7.918084E-4 26.923079 57 AGATCTC 95 9.307598E-6 25.789473 40 TGTAGAT 125 1.0833355E-7 25.199999 37 GTGTAGA 120 2.1424185E-6 23.333332 36 ATGCCGA 80 0.0026535324 21.875 18 GGTGTAG 100 3.9056863E-4 21.000002 35 GACGAAC 100 3.9056863E-4 21.000002 23 ACCAGAC 85 0.003771512 20.588236 33 GATGCCG 85 0.003771512 20.588236 17 ATTGAAC 390 0.0 18.846153 70 >>END_MODULE