FastQCFastQC Report
Wed 25 May 2016
SRR1780282_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780282_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121188
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT84036.933854837112586No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC24472.019176816186421No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG23831.966366306895072No Hit
CACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTTCTG20071.6561045648083967RNA PCR Primer, Index 15 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC5410.44641383635343435No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT2540.20959170875004124RNA PCR Primer, Index 48 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT1840.15183021421262832No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC1400.11552298907482589No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1290.10644618279037528No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTGA150.002220090270.060
TCGGGTT150.002220090270.026
GTGTTTG200.00693711152.53
CGGGTTG200.00693711152.527
TTAGAGT200.00693711152.516
TTCAAGA200.00693711152.514
CTGGTTT200.00693711152.518
ACGATTT200.00693711152.552
CTAGTTG200.00693711152.526
TACGATT200.00693711152.551
AAGCTCG200.00693711152.536
CTTTATA703.783498E-1045.02
TTTATAC953.6379788E-1240.5263143
TCTTTAT753.497553E-837.3333321
CCGTCTT19850.035.7934547
CTCGTAT19950.035.61403739
TTGAAAA19500.035.5384658
GCCGTCT20000.035.52499846
ATGCCGT20200.035.51980244
TCGTATG20150.035.43424640
Produced by FastQC (version 0.11.5)