##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780282_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 121188 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.7697874377001 34.0 34.0 34.0 34.0 34.0 2 33.74762352708189 34.0 34.0 34.0 34.0 34.0 3 33.75486021718322 34.0 34.0 34.0 34.0 34.0 4 33.77809684127141 34.0 34.0 34.0 34.0 34.0 5 33.77067036340232 34.0 34.0 34.0 34.0 34.0 6 37.54206687130739 38.0 38.0 38.0 37.0 38.0 7 37.53817209624715 38.0 38.0 38.0 37.0 38.0 8 37.464872759679174 38.0 38.0 38.0 37.0 38.0 9 37.46727398752352 38.0 38.0 38.0 37.0 38.0 10-11 37.421535135491965 38.0 38.0 38.0 37.0 38.0 12-13 37.40111644717299 38.0 38.0 38.0 37.0 38.0 14-15 37.328122421361854 38.0 38.0 38.0 37.0 38.0 16-17 37.35680512922072 38.0 38.0 38.0 36.5 38.0 18-19 37.3195077070337 38.0 38.0 38.0 36.0 38.0 20-21 37.333651021553294 38.0 38.0 38.0 36.5 38.0 22-23 37.32686817176618 38.0 38.0 38.0 36.0 38.0 24-25 37.33189754761197 38.0 38.0 38.0 36.5 38.0 26-27 37.22948641779714 38.0 38.0 38.0 36.0 38.0 28-29 37.206154899825066 38.0 38.0 38.0 36.0 38.0 30-31 37.21472010430075 38.0 38.0 38.0 36.0 38.0 32-33 37.15607568406113 38.0 38.0 38.0 36.0 38.0 34-35 37.145315542793014 38.0 38.0 38.0 36.0 38.0 36-37 37.12486797372677 38.0 38.0 38.0 36.0 38.0 38-39 36.606004719939264 38.0 37.0 38.0 34.0 38.0 40-41 36.85281958609764 38.0 37.5 38.0 35.0 38.0 42-43 37.0913374261478 38.0 38.0 38.0 36.0 38.0 44-45 37.15789104531802 38.0 38.0 38.0 36.0 38.0 46-47 37.112172822391656 38.0 38.0 38.0 36.0 38.0 48-49 37.13300821863551 38.0 38.0 38.0 36.0 38.0 50-51 36.978537478958316 38.0 38.0 38.0 35.5 38.0 52-53 36.98595983100637 38.0 38.0 38.0 36.0 38.0 54-55 36.95494190843978 38.0 38.0 38.0 35.5 38.0 56-57 36.99182674852295 38.0 38.0 38.0 36.0 38.0 58-59 36.89382199557712 38.0 38.0 38.0 35.5 38.0 60-61 36.88628412053998 38.0 38.0 38.0 35.0 38.0 62-63 36.86473495725649 38.0 38.0 38.0 35.0 38.0 64-65 36.77871571442717 38.0 38.0 38.0 35.0 38.0 66-67 36.458180677954914 38.0 37.5 38.0 34.0 38.0 68-69 36.37700927484569 38.0 37.0 38.0 34.0 38.0 70-71 35.66753308908473 38.0 37.0 38.0 30.5 38.0 72-73 34.42837574677361 38.0 36.5 38.0 22.5 38.0 74-75 34.06278674456217 38.0 36.0 38.0 21.0 38.0 76 33.405980790177246 38.0 34.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.027343121545420956 1101 2 -0.0520418755548917 1101 3 -0.08598771587352161 1101 4 0.09601140139439934 1101 5 -0.126676956277322 1101 6 -0.3920593221012396 1101 7 -0.01611080591687397 1101 8 -0.07303673546186928 1101 9 0.052826842805444585 1101 10-11 0.17350831385741827 1101 12-13 -0.014430311286496078 1101 14-15 -0.029223142760891108 1101 16-17 -0.028581326706778043 1101 18-19 0.1893791970350307 1101 20-21 0.03708395651297991 1101 22-23 -0.22887990495760135 1101 24-25 -0.021472005166138786 1101 26-27 0.0952682012237176 1101 28-29 0.01962355118236303 1101 30-31 -0.06899658039956336 1101 32-33 -0.16597817765849499 1101 34-35 0.1343389606116432 1101 36-37 -0.010203776058780534 1101 38-39 0.061860648642287686 1101 40-41 0.24909533197815392 1101 42-43 0.12458230245337631 1101 44-45 0.01077879324225961 1101 46-47 -0.012829616402363797 1101 48-49 0.02202490057658224 1101 50-51 -0.11020564679870404 1101 52-53 0.15116286160449732 1101 54-55 -0.1300598471899903 1101 56-57 -0.14405476337137912 1101 58-59 0.07737045655289876 1101 60-61 0.1059870678994983 1101 62-63 0.2671058642863571 1101 64-65 0.007610486097249236 1101 66-67 -0.2703796786800936 1101 68-69 -0.5327076037037131 1101 70-71 -0.2109874700098544 1101 72-73 -0.7175679095330523 1101 74-75 -0.6969984543850032 1101 76 -0.755920475614289 1102 1 0.07147904724661913 1102 2 0.04537014217851265 1102 3 -0.07311374685626504 1102 4 0.009160299143232464 1102 5 -0.04382297285420833 1102 6 0.019998991195777194 1102 7 0.02388067230694446 1102 8 0.0025635932352088275 1102 9 -0.006987910012320242 1102 10-11 -0.149588743086305 1102 12-13 0.08877448525609566 1102 14-15 -0.07177622632502079 1102 16-17 -0.09884540031870159 1102 18-19 -0.06924149262014367 1102 20-21 -0.13936246281927822 1102 22-23 -0.05662843140046192 1102 24-25 -0.14912111088688818 1102 26-27 -0.17288876177757118 1102 28-29 -0.0455731090929703 1102 30-31 -0.1492534510816128 1102 32-33 -0.10909532164607327 1102 34-35 0.04997337641345467 1102 36-37 9.100403781019395E-4 1102 38-39 -0.27575386272515345 1102 40-41 -0.11444972691340638 1102 42-43 -0.013171195025599047 1102 44-45 -0.029080800350172353 1102 46-47 0.046269915914443516 1102 48-49 0.11574922111711317 1102 50-51 0.07005528187199417 1102 52-53 0.03981165283112631 1102 54-55 -0.039693512263902164 1102 56-57 0.08085418550810886 1102 58-59 -0.09230926669228268 1102 60-61 -0.032552664881947635 1102 62-63 0.21218098780138206 1102 64-65 0.11208847658127041 1102 66-67 -0.14516536615409592 1102 68-69 -0.018230729166575088 1102 70-71 -0.22140128340323884 1102 72-73 -0.5916992463359705 1102 74-75 -0.8010960084323457 1102 76 -0.8029119842730239 1103 1 -0.15149821556040877 1103 2 -0.016750408593281918 1103 3 -0.022143547261009644 1103 4 0.09887896110109295 1103 5 0.04231448200869181 1103 6 -0.02524722919230271 1103 7 0.1874654506887552 1103 8 6.390664307147631E-4 1103 9 0.11119192412124335 1103 10-11 0.14424384292218662 1103 12-13 0.033498900953880195 1103 14-15 0.096877795340788 1103 16-17 -0.08044831290153098 1103 18-19 0.023239956528705363 1103 20-21 0.019202673484834065 1103 22-23 0.02986615214780386 1103 24-25 -0.011199996645387955 1103 26-27 -0.05545790585915 1103 28-29 -0.08040307615777209 1103 30-31 0.11891051066594116 1103 32-33 -0.03432646326744049 1103 34-35 -0.198062415817013 1103 36-37 -0.16105277855765365 1103 38-39 0.08657798918548565 1103 40-41 0.13618516852724838 1103 42-43 0.027397631608266693 1103 44-45 0.04178940321317981 1103 46-47 -0.13768111777469727 1103 48-49 -0.1682325653199399 1103 50-51 -0.15545164245639853 1103 52-53 -0.16549766029137203 1103 54-55 -0.12237376304759806 1103 56-57 -0.17135802829292857 1103 58-59 0.084523993892617 1103 60-61 -0.12229116732233081 1103 62-63 -0.11126351836900739 1103 64-65 -0.2873897187284413 1103 66-67 -0.3045241217590302 1103 68-69 -0.035452267994401154 1103 70-71 -0.052365332448914614 1103 72-73 0.36631333252994835 1103 74-75 0.08640349555559368 1103 76 0.4311263475392053 1104 1 0.09215354932543818 1104 2 0.007667351168088032 1104 3 -0.013978799087084326 1104 4 -0.20656292960218536 1104 5 -0.10387657692281493 1104 6 -0.021719369618324436 1104 7 0.02457203220239279 1104 8 -0.07165815334824543 1104 9 -0.04740878718703101 1104 10-11 -0.2142545700190368 1104 12-13 -0.06721240731794609 1104 14-15 -0.0028296504506428732 1104 16-17 -0.07091161933772128 1104 18-19 -0.06439723801028663 1104 20-21 -0.028867998433788955 1104 22-23 -0.059133427186019105 1104 24-25 -0.06402228430457058 1104 26-27 -0.0880929970672284 1104 28-29 -0.15727982096636595 1104 30-31 -0.07360163128976893 1104 32-33 -0.40670207992581453 1104 34-35 -0.049672420023675556 1104 36-37 -0.07395097734154632 1104 38-39 -0.1095528379971995 1104 40-41 -0.3585660647895139 1104 42-43 -0.21038870821352873 1104 44-45 -0.3737331789123317 1104 46-47 -0.4264971336835188 1104 48-49 -0.13816927418069724 1104 50-51 -0.32106026993736947 1104 52-53 -0.5054724203204373 1104 54-55 -0.5321169654645175 1104 56-57 -0.6871330101278019 1104 58-59 -0.13767857599533073 1104 60-61 -0.3832189274661175 1104 62-63 -0.0979886321400869 1104 64-65 -0.3488165419339211 1104 66-67 -0.4580672782850925 1104 68-69 -0.4585085990204476 1104 70-71 -0.3019494465641799 1104 72-73 -0.11111888285113736 1104 74-75 -0.516501969280533 1104 76 -0.14679250212999762 1105 1 -0.21362561449875273 1105 2 -0.025243671149425495 1105 3 -0.03982423325529538 1105 4 -0.16486134106305883 1105 5 -0.05137682056378168 1105 6 -0.23449590212003102 1105 7 -0.008124586060937133 1105 8 -0.20977334679917448 1105 9 0.06409106662838582 1105 10-11 0.03606703606224215 1105 12-13 -0.13510052738421052 1105 14-15 -0.1366665084566776 1105 16-17 -0.11677946847523657 1105 18-19 -0.06545951264238425 1105 20-21 0.016507695942983958 1105 22-23 -0.009864350098006014 1105 24-25 -0.24831718733546637 1105 26-27 0.012028823418098966 1105 28-29 0.14307589485080285 1105 30-31 -0.058223013221358144 1105 32-33 0.13084299365375074 1105 34-35 -0.008536078536494074 1105 36-37 -0.0234149672060866 1105 38-39 -0.19030032849519785 1105 40-41 -0.0867017240819763 1105 42-43 -0.15362523486784596 1105 44-45 -0.16880675848547355 1105 46-47 -0.14690937420588313 1105 48-49 -0.10591120966456913 1105 50-51 -0.20504331252581665 1105 52-53 -0.12836005310476395 1105 54-55 0.021981075662075966 1105 56-57 -1.0445540603853942E-4 1105 58-59 0.11781309148346963 1105 60-61 0.22044055991328548 1105 62-63 -0.055965437519681416 1105 64-65 0.0678777373760866 1105 66-67 -0.21636568974596315 1105 68-69 0.14523372628897846 1105 70-71 0.2132634956441848 1105 72-73 0.2105456722604515 1105 74-75 0.1516444102146366 1105 76 -0.032368956666594784 1106 1 0.05062923426016397 1106 2 -0.11843028556003787 1106 3 -0.16160093911400253 1106 4 0.019853762946588915 1106 5 0.11667664139984169 1106 6 0.12861333313270507 1106 7 0.0359413369618764 1106 8 0.08690035433639309 1106 9 0.19271130580033002 1106 10-11 -0.02363048177516447 1106 12-13 0.13855036197364257 1106 14-15 0.1555453295495468 1106 16-17 -0.1254569883224832 1106 18-19 0.08501782631851995 1106 20-21 0.018256263926097915 1106 22-23 8.330672469796241E-4 1106 24-25 0.008546444460336033 1106 26-27 -0.20115075652361014 1106 28-29 0.012591521421001062 1106 30-31 -0.18713468039049275 1106 32-33 -0.004997625379509429 1106 34-35 -0.07436865817673066 1106 36-37 -0.20847677003997234 1106 38-39 -5.656284650541465E-4 1106 40-41 0.042973685424598784 1106 42-43 -0.062121868372017275 1106 44-45 0.14455370779932508 1106 46-47 -0.252009364156649 1106 48-49 0.12195533641120448 1106 50-51 0.057096170943182756 1106 52-53 0.198436248774442 1106 54-55 0.1194486668589434 1106 56-57 -0.03970851529951602 1106 58-59 -0.03806069004602364 1106 60-61 -0.07542417729905537 1106 62-63 0.04802411126939177 1106 64-65 0.014445921478191792 1106 66-67 0.034704554450598835 1106 68-69 0.20231341677234127 1106 70-71 0.5980891413079377 1106 72-73 1.027237462030321 1106 74-75 0.9134422194800393 1106 76 0.7662341987951677 1107 1 -0.057047598521407394 1107 2 0.1676271954700539 1107 3 -0.07311374685626504 1107 4 0.08126061262285589 1107 5 -0.04068817661596569 1107 6 0.16419961815502404 1107 7 0.2025640578868817 1107 8 0.2408081073417918 1107 9 0.10586475456447886 1107 10-11 0.08395357666291403 1107 12-13 0.1201224476385434 1107 14-15 0.2652143692862623 1107 16-17 0.04065303228318129 1107 18-19 0.026369792646313783 1107 20-21 0.19762813279201197 1107 22-23 0.14713332408542357 1107 24-25 0.18159989224790962 1107 26-27 0.29262847960173843 1107 28-29 0.28985008839919146 1107 30-31 0.06704748935726457 1107 32-33 0.28745640249185556 1107 34-35 0.06721475572379632 1107 36-37 0.3441702284658774 1107 38-39 0.3041834413500837 1107 40-41 0.25388883108031735 1107 42-43 0.286201845726751 1107 44-45 0.31574678585672444 1107 46-47 0.13090941434705172 1107 48-49 0.3775047070105231 1107 50-51 0.32240637905068326 1107 52-53 0.22476463088754883 1107 54-55 0.16093344698374779 1107 56-57 0.146684906511247 1107 58-59 0.3700731784487914 1107 60-61 0.03798025047855447 1107 62-63 0.34384242980765833 1107 64-65 0.13716684648722577 1107 66-67 0.2153362012440212 1107 68-69 0.5005780482628879 1107 70-71 0.15947645954346967 1107 72-73 0.5869841392439739 1107 74-75 0.702038787805904 1107 76 0.9525739091439007 1108 1 -0.08973075189421564 1108 2 -0.010030608624532533 1108 3 0.020988192073261303 1108 4 -0.2591066616524671 1108 5 0.012661411216384977 1108 6 -0.29739912792648227 1108 7 -0.021864132058546204 1108 8 -0.12496649693856199 1108 9 -0.13118969800721203 1108 10-11 -0.002732247091877582 1108 12-13 -0.25861705466417817 1108 14-15 -0.029567743024578874 1108 16-17 -0.08605465764289733 1108 18-19 0.13382364147947357 1108 20-21 -0.029828425124314606 1108 22-23 0.14433716324328572 1108 24-25 0.10096596678639713 1108 26-27 0.1568018222233576 1108 28-29 0.14324515012758354 1108 30-31 0.02080924484315716 1108 32-33 -0.21314346592410516 1108 34-35 -0.3451947802489599 1108 36-37 -0.057833525549369824 1108 38-39 0.23917995824314886 1108 40-41 0.1273165953126778 1108 42-43 0.141048202009884 1108 44-45 -0.24407557568977012 1108 46-47 -0.060579226854237334 1108 48-49 -0.12891001492144483 1108 50-51 -0.19969683701805252 1108 52-53 -0.19692835258704378 1108 54-55 -0.19336141736858536 1108 56-57 0.10091107625465412 1108 58-59 0.10995765540222635 1108 60-61 0.07885354278862877 1108 62-63 -0.23221991780641815 1108 64-65 0.16576702451268233 1108 66-67 0.16068528596092335 1108 68-69 -0.2675002442406935 1108 70-71 -0.34287947528373763 1108 72-73 -0.42056743008920705 1108 74-75 -0.741167218618493 1108 76 -0.4271718240501059 1109 1 -0.07179093707939899 1109 2 -0.03522350985910094 1109 3 0.06844189577696369 1109 4 0.03483623958210558 1109 5 -0.03675988507990979 1109 6 -0.25052412792648227 1109 7 0.04711734942293333 1109 8 -0.06138625002498088 1109 9 0.1603612279187132 1109 10-11 -0.1145378026474333 1109 12-13 -0.009697301577766382 1109 14-15 0.07739599154331955 1109 16-17 0.0956197250731563 1109 18-19 0.19094169703502928 1109 20-21 0.1600056798139562 1109 22-23 -0.13874539848510636 1109 24-25 0.10808402234194858 1109 26-27 0.023873581482611428 1109 28-29 -0.03827491160080854 1109 30-31 -0.013565755156839998 1109 32-33 0.007959929137619781 1109 34-35 0.13057682468931375 1109 36-37 0.11151446827778244 1109 38-39 0.04977023602093311 1109 40-41 0.09575795333737602 1109 42-43 -0.035379267125918545 1109 44-45 0.029882757643555635 1109 46-47 0.14275796450379374 1109 48-49 0.15401822581929991 1109 50-51 0.15261797779676556 1109 52-53 0.0709343017339421 1109 54-55 -0.2208112630476009 1109 56-57 0.0039782058842874335 1109 58-59 0.0645872850318625 1109 60-61 0.13188209217134528 1109 62-63 -0.03343519558419672 1109 64-65 -0.24235411746261804 1109 66-67 0.13958189089920126 1109 68-69 0.17441719403091582 1109 70-71 0.5130618827409563 1109 72-73 0.24139244645400737 1109 74-75 0.49322707150496825 1109 76 0.8606753981721127 1110 1 -0.06106978323324341 1110 2 -0.1876754329360253 1110 3 0.030845741930811243 1110 4 0.040845854966718775 1110 5 -0.03286565431068311 1110 6 -0.34124528177263613 1110 7 -0.29730572750014517 1110 8 -0.1167708654095918 1110 9 -0.08910992592744549 1110 10-11 -0.15790318726281782 1110 12-13 -0.025274224654687316 1110 14-15 -0.07666650845668244 1110 16-17 0.09307164815007951 1110 18-19 0.14937919703503155 1110 20-21 0.029212410583177473 1110 22-23 -0.2555963600235671 1110 24-25 -0.10347847765804374 1110 26-27 0.07106108148261825 1110 28-29 0.00831162686073128 1110 30-31 -0.1507292166953036 1110 32-33 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0.05062180173394637 2115 54-55 0.10575123695240052 2115 56-57 0.08835320588428175 2115 58-59 0.22552478503186535 2115 60-61 0.10375709217134954 2115 62-63 0.06500230441580612 2115 64-65 0.027958382537384807 2115 66-67 -0.05729310910080443 2115 68-69 0.0587921940309144 2115 70-71 -0.11193811725904368 2115 72-73 0.441392446453996 2115 74-75 0.18697707150496257 2115 76 0.4325503981721184 2116 1 -0.08894032732330004 2116 2 0.048084416970162636 2116 3 0.026521164069649217 2116 4 0.10960758104551616 2116 5 0.08831633443228526 2116 6 -0.00776498158501937 2116 7 0.06632466649610791 2116 8 0.06605277436526791 2116 9 -0.16562962573982531 2116 10-11 -0.049141461184021296 2116 12-13 -0.13103876499238964 2116 14-15 0.03223593056771534 2116 16-17 -0.20232234809757443 2116 18-19 -0.06976714442838983 2116 20-21 0.12338220420419788 2116 22-23 0.0653323454173318 2116 24-25 -0.2489662825360952 2116 26-27 0.09593913026310474 2116 28-29 -0.11106454574715485 2116 30-31 -0.021873681986114946 2116 32-33 -0.07367116842335264 2116 34-35 -0.019728053359465036 2116 36-37 -0.14225839757587266 2116 38-39 -0.10651787373517152 2116 40-41 -0.44860180276018014 2116 42-43 -0.19140060858932628 2116 44-45 -0.33147394967351573 2116 46-47 -0.11109112086206352 2116 48-49 -0.025364396131919875 2116 50-51 -0.2095771441544585 2116 52-53 -0.11069679582703174 2116 54-55 0.1581140418304514 2116 56-57 0.10554070588428743 2116 58-59 0.044465333812347296 2116 60-61 -0.18172309075548299 2116 62-63 -0.07859525655980093 2116 64-65 -0.024480641852868246 2116 66-67 -0.0565309139788539 2116 68-69 -0.11174896450567218 2116 70-71 0.114738712009256 2116 72-73 -0.4002234072045354 2116 74-75 -0.17716164800723533 2116 76 -0.12568130914495868 2117 1 0.13417497201150752 2117 2 0.017156976034314653 2117 3 -0.022957007044354327 2117 4 0.12828255619652396 2117 5 0.0564905067696202 2117 6 0.15166043320204636 2117 7 0.24958600146054977 2117 8 -0.21687214344190409 2117 9 0.1309431244704342 2117 10-11 0.2297216017412822 2117 12-13 0.03391555108681388 2117 14-15 0.1601986953050769 2117 16-17 0.13783171566876717 2117 18-19 -0.06140450202452996 2117 20-21 -0.031211992599843086 2117 22-23 0.22863802627978913 2117 24-25 0.08912340321969481 2117 26-27 0.3600265987239979 2117 28-29 -0.3888332971807458 2117 30-31 0.1751979595766997 2117 32-33 0.057048878980886286 2117 34-35 0.2553025300184615 2117 36-37 -0.025735727646981843 2117 38-39 -0.09393568090696647 2117 40-41 -0.1301237081046338 2117 42-43 0.21410153224712758 2117 44-45 0.06809788303541353 2117 46-47 -0.08695892421093987 2117 48-49 -0.004940434055299647 2117 50-51 0.16880136337670137 2117 52-53 0.13385553997845534 2117 54-55 0.2675165190840616 2117 56-57 -0.030431080949568923 2117 58-59 0.031515184718379885 2117 60-61 -0.0936811915277147 2117 62-63 0.3203314580208172 2117 64-65 0.06976872736496631 2117 66-67 -0.07463245079359382 2117 68-69 0.11186331472056565 2117 70-71 0.02938241565631472 2117 72-73 -0.3973418795648058 2117 74-75 0.04529897589367948 2117 76 -0.5489934889752206 2118 1 -0.1334065031171363 2118 2 -0.05906391866413685 2118 3 -0.036314393531213796 2118 4 -0.10472743966317921 2118 5 -0.20816318696670777 2118 6 0.018638607922575545 2118 7 0.01307646891979175 2118 8 0.013456517270618917 2118 9 -0.04960339472279429 2118 10-11 0.03998263760413323 2118 12-13 0.05736244684991476 2118 14-15 -0.028490407827753472 2118 16-17 0.08911422192850438 2118 18-19 -0.12980319290207376 2118 20-21 -0.11356939880240446 2118 22-23 -0.1642465777303883 2118 24-25 0.030735358819946157 2118 26-27 -0.09258671725952183 2118 28-29 -0.03247695562596675 2118 30-31 0.0019413203148559433 2118 32-33 0.029402539200511058 2118 34-35 0.13395732783396141 2118 36-37 0.1553332575859656 2118 38-39 -0.47596679228095695 2118 40-41 -0.24421846175696516 2118 42-43 -0.17321338662276986 2118 44-45 -0.04596236814260379 2118 46-47 -0.04453566757168659 2118 48-49 -0.07055921128761611 2118 50-51 -0.02403296559946 2118 52-53 0.04937376714274677 2118 54-55 -0.1373108699658374 2118 56-57 0.026138190161013597 2118 58-59 -0.017488186666255956 2118 60-61 -0.1728643700928103 2118 62-63 -0.20418008552130118 2118 64-65 -0.4007365860160803 2118 66-67 0.12804494121366616 2118 68-69 -0.05328524307600446 2118 70-71 -0.38343968958608343 2118 72-73 -0.8608481195837356 2118 74-75 -0.8441255228346662 2118 76 -0.7568363942807181 2119 1 0.10521024588589967 2119 2 -0.0064281628559470505 2119 3 0.12059094940472193 2119 4 0.09296912601743657 2119 5 0.052327260030494926 2119 6 0.1718437742817187 2119 7 -0.05457823417327745 2119 8 0.05911848183622226 2119 9 -0.05463285725478784 2119 10-11 0.10772166107496162 2119 12-13 -0.07203957287114093 2119 14-15 -0.3382437955229207 2119 16-17 -0.01827025915397229 2119 18-19 0.02534134214543826 2119 20-21 0.08115611356789287 2119 22-23 0.15647837911741647 2119 24-25 0.1912218377993682 2119 26-27 0.168032690315421 2119 28-29 0.06902989912474311 2119 30-31 -0.13297624411583087 2119 32-33 -0.14510592259739497 2119 34-35 0.08124717169246054 2119 36-37 0.07098311969735249 2119 38-39 -0.19006119142386524 2119 40-41 0.040020571633903046 2119 42-43 0.02851071710121289 2119 44-45 -0.025095554659280594 2119 46-47 0.11645177365205228 2119 48-49 0.021136719510153057 2119 50-51 0.0216637191216833 2119 52-53 -0.018290855994763433 2119 54-55 0.04205841360855089 2119 56-57 0.19031988569501124 2119 58-59 0.004177190394635488 2119 60-61 -0.028572363664622458 2119 62-63 -0.020644383281357648 2119 64-65 -0.09187600232066728 2119 66-67 -0.02762039616705181 2119 68-69 -0.05386060565362527 2119 70-71 0.20902008463370692 2119 72-73 -0.3745973011800672 2119 74-75 -0.21707951366853706 2119 76 -1.1205647437837172 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 1.0 21 1.0 22 7.0 23 19.0 24 24.0 25 64.0 26 93.0 27 193.0 28 355.0 29 610.0 30 916.0 31 1441.0 32 2409.0 33 3784.0 34 6381.0 35 11085.0 36 17033.0 37 76771.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.956926428359246 13.879261973132653 11.0241938145691 35.139617783939 2 22.43703997095422 18.069445819718126 33.07835759316104 26.41515661616662 3 20.033336633990164 16.57012245436842 32.12199227646302 31.2745486351784 4 15.45532560979635 22.067366405914775 31.016272238175397 31.461035746113474 5 16.025514077301384 27.97801762550748 37.92454698484998 18.071921312341154 6 29.540878634848333 36.260190777964816 16.431494867478627 17.76743571970822 7 28.496220747928835 24.532131894246955 20.270983925801232 26.700663432022974 8 24.99092319371555 35.72135855035152 15.2168531537776 24.070865102155327 9 23.166485130540977 16.5816747532759 15.660791497508002 44.59104861867512 10-11 26.40938046671288 25.52150377925207 22.972984123840646 25.096131630194407 12-13 25.622173812588706 18.362379113443573 25.35564577350893 30.65980130045879 14-15 22.988662243786514 17.500907680628444 20.10636366636961 39.40406640921543 16-17 28.1628544080272 24.330791827573687 18.61157870416213 28.894775060236988 18-19 30.366042842525665 22.849622074792883 19.36082780473314 27.42350727794831 20-21 25.21330494768459 20.610951579364293 19.243241905139122 34.932501567812 22-23 23.537809023995777 24.456629369244478 20.113377562134865 31.892184044624884 24-25 28.43763408918375 24.550698088919695 20.93565369508532 26.076014126811238 26-27 29.751295507806052 26.17008284648645 19.81590586526719 24.26271578044031 28-29 24.36091032115391 21.927913654817306 24.80196059015744 28.90921543387134 30-31 23.077779978215666 20.7145096874278 31.26134600785556 24.946364326500976 32-33 26.241046968346698 19.72596296663036 24.99174835792323 29.04124170709971 34-35 25.529755421328844 19.335247714295146 26.265389312473182 28.869607551902828 36-37 23.80186157045252 27.605455985741163 22.426312836254414 26.166369607551903 38-39 22.176288081328185 24.531719312143117 26.21340396738951 27.07858863913919 40-41 22.91192197247252 18.80920553190085 27.7114895864277 30.56738290919893 42-43 27.4948839819124 18.412301548008053 26.69199920784236 27.40081526223718 44-45 22.376390401689935 23.564214278641447 29.59121365151665 24.46818166815196 46-47 25.732333234313625 19.98258903521801 26.992771561540746 27.292306168927617 48-49 25.334604086213158 18.599201241046966 23.877776677558835 32.18841799518104 50-51 23.150394428491268 19.56959434927551 27.589777865795295 29.69023335643793 52-53 19.407862164570748 19.572895006106215 34.480311582004816 26.538931247318214 54-55 19.951645377430108 20.4232267221177 30.1135425949764 29.51158530547579 56-57 23.682212760339308 21.441479354391525 24.97565765587352 29.900650229395648 58-59 20.492953097666437 21.441891936495363 30.51746047463445 27.54769449120375 60-61 23.87035019968974 25.030118493580222 25.598656632669904 25.500874674060135 62-63 19.9537082879493 32.820081196158036 21.26365646763706 25.962554048255605 64-65 19.22838894940093 34.75880450209592 20.25365547744001 25.75915107106314 66-67 19.23168960623164 34.242664290193744 19.67026438261214 26.855381720962473 68-69 18.878931907449584 34.402746146483146 20.19218074396805 26.526141202099218 70-71 19.190843977951612 33.10311251939136 20.714922269531638 26.99112123312539 72-73 19.32699607221837 30.083836683500014 21.972885104135724 28.616282140145888 74-75 19.43880582235865 28.719015084001715 22.974634452255998 28.867544641383635 76 19.5332871241377 28.733868039739907 22.988249661682676 28.744595174439713 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 2.0 16 4.5 17 6.0 18 7.0 19 6.0 20 10.5 21 17.0 22 27.0 23 49.5 24 83.5 25 105.0 26 125.5 27 179.5 28 275.0 29 337.0 30 393.5 31 537.5 32 796.0 33 967.0 34 1037.0 35 1287.0 36 1633.5 37 1800.0 38 2139.5 39 4778.5 40 8246.5 41 8910.5 42 8406.0 43 7641.0 44 6384.0 45 5662.5 46 5433.0 47 5379.5 48 5107.5 49 4741.0 50 4593.0 51 4228.0 52 3651.5 53 3203.5 54 2967.0 55 2807.5 56 2544.0 57 2467.5 58 2495.0 59 2390.5 60 2295.5 61 2303.5 62 2302.0 63 2380.0 64 2491.0 65 2578.0 66 2636.5 67 2641.0 68 2776.0 69 2772.5 70 2597.5 71 2561.0 72 2496.0 73 2351.0 74 2086.0 75 1901.0 76 1680.5 77 1238.0 78 853.5 79 691.0 80 531.5 81 250.0 82 89.0 83 50.0 84 30.5 85 7.0 86 3.0 87 3.0 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 121188.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.9701950688187 #Duplication Level Percentage of deduplicated Percentage of total 1 64.44073737067367 31.556754794204046 2 20.84386479290938 20.414562497937087 3 7.181612914096991 10.550549559362313 4 2.9757692178074344 5.828959963032643 5 1.428908435277862 3.49869624055187 6 0.88295757085566 2.5943162689375185 7 0.5560610656152057 1.9061293197346272 8 0.3976679135914805 1.557910024094795 9 0.28477066693627207 1.2550747598772156 >10 0.9722643480605263 6.560880615242433 >50 0.02022040238600748 0.6708585008416675 >100 0.0067401341286691605 0.5833910948278708 >500 0.0016850335321672901 0.44641383635343435 >1k 0.00505510059650187 5.64164768788989 >5k 0.0016850335321672901 6.933854837112586 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT 8403 6.933854837112586 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 2447 2.019176816186421 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG 2383 1.966366306895072 No Hit CACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTTCTG 2007 1.6561045648083967 RNA PCR Primer, Index 15 (96% over 25bp) TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 541 0.44641383635343435 No Hit ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT 254 0.20959170875004124 RNA PCR Primer, Index 48 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT 184 0.15183021421262832 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 140 0.11552298907482589 No Hit CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG 129 0.10644618279037528 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0024754926230319834 0.0 2 0.0 0.0 0.0 0.0024754926230319834 0.0 3 0.0 0.0 0.0 0.003300656830709311 0.0 4 0.0 0.0 0.0 0.009076806284450605 0.0 5 0.0 8.251642076773278E-4 0.0 0.013202627322837245 0.0 6 0.0 8.251642076773278E-4 0.0 0.015678119945869227 0.0 7 0.0 8.251642076773278E-4 0.0 0.015678119945869227 0.0 8 0.0 8.251642076773278E-4 0.0 0.016503284153546557 0.0 9 0.0 8.251642076773278E-4 0.0 0.01897877677657854 0.0 10 0.0 8.251642076773278E-4 0.0 0.025580090437997163 0.0 11 0.0 8.251642076773278E-4 0.0 0.03053107568406113 0.0 12 0.0 8.251642076773278E-4 0.0 0.03053107568406113 0.0 13 0.0 8.251642076773278E-4 0.0 0.03465689672244777 0.0 14 0.0 8.251642076773278E-4 0.0 0.0354820609301251 0.0 15 0.0 8.251642076773278E-4 0.0 0.03630722513780242 0.0 16 0.0 8.251642076773278E-4 0.0 0.03713238934547975 0.0 17 0.0 8.251642076773278E-4 0.0 0.039607881968511734 0.0 18 0.0 8.251642076773278E-4 0.0 0.04620919562993036 0.0 19 0.0 8.251642076773278E-4 0.0 0.05281050929134898 0.0 20 0.0 8.251642076773278E-4 0.0 0.06023698716044493 0.0 21 0.0 8.251642076773278E-4 0.0 0.06106215136812226 0.0 22 0.0 8.251642076773278E-4 0.0 0.07013895765257286 0.0 23 0.0 8.251642076773278E-4 0.0 0.0742647786909595 0.0 24 0.0 8.251642076773278E-4 0.0 0.07756543552166881 0.0 25 0.0 8.251642076773278E-4 0.0 0.08746740601379674 0.0 26 0.0 8.251642076773278E-4 0.0 0.10892167541340726 0.0 27 0.0 8.251642076773278E-4 0.0 0.19473875301184937 0.0 28 0.0 8.251642076773278E-4 0.0 0.2879823084793874 0.0 29 0.0 8.251642076773278E-4 0.0 0.4101066112156319 0.0 30 0.0 8.251642076773278E-4 0.0 0.5429580486516817 0.0 31 0.0 8.251642076773278E-4 0.0 0.6758094860877315 0.0 32 0.0 8.251642076773278E-4 0.0 0.8111364161468132 0.0 33 0.0 8.251642076773278E-4 0.0 1.0157771396507904 0.0 34 0.0 8.251642076773278E-4 0.0 1.2509489388388288 0.0 35 0.0 8.251642076773278E-4 0.0 1.5900914281942107 0.0 36 0.0 8.251642076773278E-4 0.0 2.0860151170082846 0.0 37 0.0 8.251642076773278E-4 0.0 2.7560484536422747 0.0 38 0.0 8.251642076773278E-4 0.0 3.435983760768393 0.0 39 0.0 8.251642076773278E-4 0.0 4.233092385384691 0.0 40 0.0 8.251642076773278E-4 0.0 4.995544113278543 0.0 41 0.0 8.251642076773278E-4 0.0 6.032775522328944 0.0 42 0.0 8.251642076773278E-4 0.0 7.106314156517147 0.0 43 0.0 8.251642076773278E-4 0.0 8.237614285242763 0.0 44 0.0 8.251642076773278E-4 0.0 9.27319536587781 0.0 45 0.0 8.251642076773278E-4 0.0 10.246063966729379 0.0 46 0.0 8.251642076773278E-4 0.0 11.182625342443146 0.0 47 0.0 8.251642076773278E-4 0.0 12.085354985642143 0.0 48 0.0 8.251642076773278E-4 0.0 12.945176090041919 0.0 49 0.0 8.251642076773278E-4 0.0 13.686173548536159 0.0 50 0.0 8.251642076773278E-4 0.0 14.381786975608145 0.0 51 0.0 8.251642076773278E-4 0.0 15.178070436016768 0.0 52 0.0 8.251642076773278E-4 0.0 16.053569660362413 0.0 53 0.0 8.251642076773278E-4 0.0 17.028913753837013 0.0 54 0.0 8.251642076773278E-4 0.0 17.967125457966134 0.0 55 0.0 8.251642076773278E-4 0.0 18.82034524870449 0.0 56 0.0 8.251642076773278E-4 0.0 19.621579694359177 0.0 57 0.0 8.251642076773278E-4 0.0 20.346899032907547 0.0 58 0.0 8.251642076773278E-4 0.0 21.008680727464764 0.0 59 0.0 8.251642076773278E-4 0.0 21.629204211638115 0.0 60 0.0 8.251642076773278E-4 0.0 22.22332244116579 0.0 61 0.0 8.251642076773278E-4 0.0 22.84467108954682 0.0 62 0.0 8.251642076773278E-4 0.0 23.52130573984223 0.0 63 0.0 8.251642076773278E-4 0.0 24.20701719642209 0.0 64 0.0 8.251642076773278E-4 0.0 24.9636927748622 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCTGA 15 0.0022200902 70.0 60 TCGGGTT 15 0.0022200902 70.0 26 GTGTTTG 20 0.006937111 52.5 3 CGGGTTG 20 0.006937111 52.5 27 TTAGAGT 20 0.006937111 52.5 16 TTCAAGA 20 0.006937111 52.5 14 CTGGTTT 20 0.006937111 52.5 18 ACGATTT 20 0.006937111 52.5 52 CTAGTTG 20 0.006937111 52.5 26 TACGATT 20 0.006937111 52.5 51 AAGCTCG 20 0.006937111 52.5 36 CTTTATA 70 3.783498E-10 45.0 2 TTTATAC 95 3.6379788E-12 40.526314 3 TCTTTAT 75 3.497553E-8 37.333332 1 CCGTCTT 1985 0.0 35.79345 47 CTCGTAT 1995 0.0 35.614037 39 TTGAAAA 1950 0.0 35.53846 58 GCCGTCT 2000 0.0 35.524998 46 ATGCCGT 2020 0.0 35.519802 44 TCGTATG 2015 0.0 35.434246 40 >>END_MODULE