FastQCFastQC Report
Wed 25 May 2016
SRR1780281_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780281_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441666
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC502.3611865E-630.7929425
TACTCTA300.00576666629.306695
CATCGTA554.925645E-628.01897824
GGTACGA407.062428E-427.4750231
GGTACAT407.062428E-427.4750231
ACTGGCG551.5865234E-424.0108212
GAAAATT602.8732268E-421.99495926
CTATACA703.2319884E-521.9800191
TAACACC500.00259150621.9800194
ATAGTCC500.00259150621.9800193
GTGGCAC755.443615E-520.54259312
ATCACTA654.887903E-420.33067715
CACGACA654.902883E-420.32145534
TGCATAA550.00446164620.01355614
AAGATTC550.00446164620.01355639
TGTATAA550.004502819.9818342
TACACGA809.019242E-519.23687232
ACGTACT708.075285E-418.86992340
TCTAATA600.00744375118.316682
ACGAGAG851.4208324E-418.12992724
Produced by FastQC (version 0.11.5)