SRR1780281_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780281_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	441666
Sequences flagged as poor quality	0
Sequence length	50
%GC	59

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT	20238	4.582195595766938	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC	5978	1.3535114769984558	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG	5589	1.2654358723560337	No Hit
CACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTTCTG	4400	0.9962279188345944	RNA PCR Primer, Index 13 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC	1178	0.26671738372435283	No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT	577	0.13064170662899113	TruSeq Adapter, Index 8 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATAC	60	3.6379788E-12	40.339973	3
CTTTATA	165	0.0	40.006588	2
CGTCTTC	530	0.0	35.695908	42
GCTTATA	25	0.0023492821	35.2058	3
CTGGATT	25	0.002351894	35.197826	14
GTCTTCT	555	0.0	34.087982	43
TCTTCTG	550	0.0	33.9979	44
TCTTTAT	195	0.0	33.897793	1
ATGCCGT	2945	0.0	33.539524	44
CCGTCTT	560	0.0	33.390793	41
ATTCCGA	40	1.8182387E-5	32.997963	19
CCTTATA	35	3.1859646E-4	31.476519	1
CCGATAA	35	3.2157468E-4	31.42663	22
TTCCGAT	35	3.2157468E-4	31.42663	20
TTTATAC	250	0.0	30.80507	3
GCCGTCT	625	0.0	29.91815	40
GTTTACG	30	0.0057428502	29.331522	9
TATGCCG	3485	0.0	28.531954	43
GCCACTA	55	4.9540777E-6	27.998272	28
TATGTCA	55	4.9540777E-6	27.998272	40