FastQCFastQC Report
Wed 25 May 2016
SRR1780280_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780280_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120033
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGAA150.002220038670.046
CGTGGTA200.006936949752.529
TATGGAC200.006936949752.521
GTCGTAA200.006936949752.51
CATACGC200.006936949752.523
ATGGTTC200.006936949752.512
TGCTACA200.006936949752.543
GTAGCGT350.001251280540.025
GTTTTAC350.001251280540.035
AGGTGGG400.002410838135.015
GAGAAGT552.977618E-431.8181847
GTCTTAG450.00429324931.11111330
ACAGACA500.00718506528.0000022
AGGGGTA500.00718506528.00000212
CTCACAA700.001220162425.030
TGGTTCT700.001220162425.013
TATACCC700.001220162425.03
ACTTTAC750.001823525623.33333452
AGAAGTA800.002653386221.87548
GGGAGAG800.002653386221.87536
Produced by FastQC (version 0.11.5)