##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780280_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 120033 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.988719768730267 34.0 31.0 34.0 12.0 34.0 2 30.008980863595845 34.0 31.0 34.0 12.0 34.0 3 29.776619762898537 34.0 31.0 34.0 11.0 34.0 4 29.729624353302842 34.0 31.0 34.0 11.0 34.0 5 29.710829521881482 34.0 31.0 34.0 11.0 34.0 6 32.55663026001183 37.0 31.0 38.0 11.0 38.0 7 32.78910799530129 37.0 33.0 38.0 12.0 38.0 8 32.85878050202861 37.0 34.0 38.0 11.0 38.0 9 33.19800388226571 38.0 34.0 38.0 12.0 38.0 10-11 32.884840002332695 38.0 33.5 38.0 11.0 38.0 12-13 32.98012213308007 38.0 34.0 38.0 11.0 38.0 14-15 32.86844034557163 38.0 34.0 38.0 11.0 38.0 16-17 32.95295876967167 38.0 34.0 38.0 11.0 38.0 18-19 32.97327818183333 38.0 34.0 38.0 11.0 38.0 20-21 32.98367115709846 38.0 34.0 38.0 11.0 38.0 22-23 32.959673589762815 38.0 34.0 38.0 11.0 38.0 24-25 32.973769713328835 38.0 34.0 38.0 11.0 38.0 26-27 32.94049553039581 38.0 34.0 38.0 11.0 38.0 28-29 33.00026242783234 38.0 34.0 38.0 11.0 38.0 30-31 33.00657735789325 38.0 34.0 38.0 11.0 38.0 32-33 33.082402339356676 38.0 34.0 38.0 11.0 38.0 34-35 33.04889905276049 38.0 34.0 38.0 11.0 38.0 36-37 32.99500137462198 38.0 34.0 38.0 11.0 38.0 38-39 32.99611773428974 38.0 34.0 38.0 11.0 38.0 40-41 33.0442045104263 38.0 34.0 38.0 11.0 38.0 42-43 33.1185798905301 38.0 34.0 38.0 11.0 38.0 44-45 33.19122657935735 38.0 34.0 38.0 11.0 38.0 46-47 33.108507660393386 38.0 34.0 38.0 11.0 38.0 48-49 33.055513900344074 38.0 34.0 38.0 11.0 38.0 50-51 33.0147834345555 38.0 34.0 38.0 11.0 38.0 52-53 33.06340339739904 38.0 34.0 38.0 11.0 38.0 54-55 33.021094199095245 38.0 34.0 38.0 11.0 38.0 56-57 32.971195421259154 38.0 33.5 38.0 11.0 38.0 58-59 33.03062074596153 38.0 34.0 38.0 11.0 38.0 60-61 33.05112760657486 38.0 33.5 38.0 11.0 38.0 62-63 32.90123132805145 38.0 33.0 38.0 11.0 38.0 64-65 32.837869585863885 38.0 33.0 38.0 11.0 38.0 66-67 32.750122882873875 38.0 32.0 38.0 11.0 38.0 68-69 32.65507402131081 38.0 32.0 38.0 11.0 38.0 70-71 32.67336065915207 38.0 32.0 38.0 11.0 38.0 72-73 32.599060258428935 38.0 32.0 38.0 11.0 38.0 74-75 32.603896428482166 38.0 32.0 38.0 11.0 38.0 76 31.096048586638673 36.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.17796797643957873 1101 2 0.4798079860827791 1101 3 0.5557279126971544 1101 4 0.11573097965809254 1101 5 -0.21818312139096108 1101 6 0.18007755579645135 1101 7 -0.08008724109993182 1101 8 -1.0454509102507608 1101 9 -0.15355383149697133 1101 10-11 0.10955871376138049 1101 12-13 0.07329594105883075 1101 14-15 0.3891581412449696 1101 16-17 0.08087301289604198 1101 18-19 -0.11217848346904447 1101 20-21 0.22095476963085048 1101 22-23 0.18357540659011562 1101 24-25 0.34945732968690635 1101 26-27 0.04627050410621081 1101 28-29 -0.19762708924744032 1101 30-31 0.060167672886223045 1101 32-33 -0.013148152264577107 1101 34-35 0.12626853485184597 1101 36-37 0.0853687898958384 1101 38-39 0.28945984653667267 1101 40-41 0.4738906742492972 1101 42-43 -0.2537283885065804 1101 44-45 0.19031792456537744 1101 46-47 0.13601961990078593 1101 48-49 0.4754341716831547 1101 50-51 0.1109038329509886 1101 52-53 0.2718062102299825 1101 54-55 0.7671301197709894 1101 56-57 0.31110319743176973 1101 58-59 0.5157772845643507 1101 60-61 -0.005691782565449444 1101 62-63 0.16583610159982243 1101 64-65 0.41836426580940866 1101 66-67 0.3132854177754112 1101 68-69 -0.18320744669949107 1101 70-71 0.5478873306121415 1101 72-73 0.2883419916579868 1101 74-75 0.27429094121562514 1101 76 -0.33254243126651417 1102 1 0.19631522172540983 1102 2 -0.05540746406877517 1102 3 -0.058878460695162005 1102 4 0.6144714564337121 1102 5 -0.3158422513983332 1102 6 -0.2505053064956755 1102 7 -0.3604489188258313 1102 8 0.05087701634872843 1102 9 -0.1407537987288876 1102 10-11 0.28048011132015915 1102 12-13 0.28396912038217437 1102 14-15 0.3067720103364664 1102 16-17 0.3125126458934986 1102 18-19 0.08455290016329542 1102 20-21 0.5124627158911963 1102 22-23 -0.06923036059264831 1102 24-25 0.19675293876365885 1102 26-27 0.2522895915150727 1102 28-29 0.13990977484692735 1102 30-31 0.032673202500383525 1102 32-33 0.4128932584014251 1102 34-35 -0.07266957442971034 1102 36-37 0.17114948949443232 1102 38-39 0.6318197629780613 1102 40-41 0.31552354882946076 1102 42-43 0.4221184616613556 1102 44-45 0.4989523146694097 1102 46-47 -0.1619600229270972 1102 48-49 -0.21019190351959338 1102 50-51 0.14563288185735246 1102 52-53 0.027796625565443378 1102 54-55 0.007320174657529321 1102 56-57 -0.13088193405016568 1102 58-59 -0.4015345037538296 1102 60-61 -0.03802466533763038 1102 62-63 -0.07904900530606085 1102 64-65 0.26076002234254503 1102 66-67 0.10207975708892292 1102 68-69 0.14413035128527696 1102 70-71 0.18138655237014945 1102 72-73 0.22308230459318423 1102 74-75 0.08131764720399559 1102 76 -0.31919967710906505 1103 1 0.4070480492014461 1103 2 0.7636220886468799 1103 3 0.6870580409022828 1103 4 0.8840002104273239 1103 5 0.21763418630134623 1103 6 -0.4236083416394436 1103 7 0.16045763069493546 1103 8 0.592690115390262 1103 9 0.755981424913287 1103 10-11 0.0017061496588155478 1103 12-13 0.46087606926396063 1103 14-15 0.37104916688598877 1103 16-17 0.6450156411011605 1103 18-19 -0.01494290654596142 1103 20-21 0.4160669491180329 1103 22-23 0.23974527838499426 1103 24-25 0.6232954707125415 1103 26-27 0.12243236308056282 1103 28-29 0.14672387229101957 1103 30-31 -0.3377890578830005 1103 32-33 -0.027811613803038426 1103 34-35 0.35239032972364726 1103 36-37 0.5425402642548107 1103 38-39 -0.08782380730948347 1103 40-41 0.5034179178390445 1103 42-43 0.3691482140575246 1103 44-45 0.4460070271294825 1103 46-47 0.14964141477258153 1103 48-49 0.48853513322162456 1103 50-51 -0.48452885935670764 1103 52-53 0.2264937102299882 1103 54-55 -0.22525770074182816 1103 56-57 0.33694454358561643 1103 58-59 0.30500004097460476 1103 60-61 0.18036590974224254 1103 62-63 -0.08007735993864173 1103 64-65 0.26011106068120426 1103 66-67 0.11997612290361559 1103 68-69 0.5313358225312825 1103 70-71 0.3440812408685474 1103 72-73 0.5839349403759329 1103 74-75 0.06583741557460598 1103 76 0.4343646200155362 1104 1 -0.35535242850555804 1104 2 -0.4916724973403781 1104 3 -0.37020708648625344 1104 4 -0.29760806982745436 1104 5 -0.6644717054184 1104 6 0.0159219131376247 1104 7 -0.5020821782222598 1104 8 -0.8406697572211996 1104 9 -0.43695249228417055 1104 10-11 -0.26624767199723465 1104 12-13 -0.15319638296534066 1104 14-15 -0.3754078394834366 1104 16-17 -0.13407472514087004 1104 18-19 -0.12283502438035754 1104 20-21 -0.9638096432386547 1104 22-23 -0.7774322693857059 1104 24-25 -0.2543418700517961 1104 26-27 -0.21657042354363654 1104 28-29 -0.4848943601930529 1104 30-31 0.12478060772209432 1104 32-33 -0.5427598622106871 1104 34-35 0.024245425265043252 1104 36-37 -0.24680089816556716 1104 38-39 -0.23182261304196317 1104 40-41 -0.18100684330745764 1104 42-43 -0.10219931778961211 1104 44-45 -0.44437911936733343 1104 46-47 -0.2765130438261494 1104 48-49 -0.45196048779748565 1104 50-51 -0.6399229676369558 1104 52-53 -0.5633848725725699 1104 54-55 -0.10752282813036373 1104 56-57 -0.09075251055450906 1104 58-59 -0.2620481278152056 1104 60-61 0.08908406260848523 1104 62-63 -0.6010367548431077 1104 64-65 -0.1872416463251696 1104 66-67 -0.07911822423014314 1104 68-69 -0.3888970596343242 1104 70-71 -0.4375568960741276 1104 72-73 -0.28921394497438513 1104 74-75 -0.34291059397953916 1104 76 -0.10848967934752096 1105 1 0.1776419933008242 1105 2 0.2772478650443979 1105 3 0.3516349042563185 1105 4 0.15938840918508745 1105 5 0.6523973850591105 1105 6 -0.14515337269534712 1105 7 0.5549063884589103 1105 8 -0.1494682697029006 1105 9 0.3404340646648336 1105 10-11 0.3626145347519838 1105 12-13 -0.31829706738199803 1105 14-15 -0.10577536727550552 1105 16-17 -0.33207092722181386 1105 18-19 0.25045887916832044 1105 20-21 -0.2777412806956363 1105 22-23 0.3317677939129382 1105 24-25 0.21447365394235618 1105 26-27 -0.20112159654676276 1105 28-29 -0.18989297553506645 1105 30-31 -0.40895559515008273 1105 32-33 -0.10318040262291817 1105 34-35 0.21578318686650277 1105 36-37 -0.060613850652018186 1105 38-39 0.201219282752632 1105 40-41 -0.08415001694356761 1105 42-43 -0.013353726936266241 1105 44-45 -0.14655896665934165 1105 46-47 0.14944731539370082 1105 48-49 0.09186393756944966 1105 50-51 0.1441396189041626 1105 52-53 0.22289316675171733 1105 54-55 0.09102723714637051 1105 56-57 0.13745890693965634 1105 58-59 -0.04225345281421511 1105 60-61 -0.10414495982297467 1105 62-63 0.01524484503029555 1105 64-65 -0.8232546225486104 1105 66-67 -0.19470749510880836 1105 68-69 0.048368043028176544 1105 70-71 0.21047293341513296 1105 72-73 -0.2901139726675481 1105 74-75 -0.6667227552328505 1105 76 -0.2866951625931584 1106 1 0.3304712143390631 1106 2 0.03382468803525995 1106 3 0.8964712672019779 1106 4 0.5517754397851213 1106 5 -0.2289158901512529 1106 6 0.4993847776266094 1106 7 -0.0962893112010903 1106 8 0.34678032945754467 1106 9 0.18313165427107947 1106 10-11 0.1630020212184462 1106 12-13 0.6347961762975984 1106 14-15 0.4922074237667218 1106 16-17 0.657472636514008 1106 18-19 0.3882919940656606 1106 20-21 0.5179878206776607 1106 22-23 0.35965162395073946 1106 24-25 0.38631630404587725 1106 26-27 0.30394516889096224 1106 28-29 0.6211361429332243 1106 30-31 0.17916908431882916 1106 32-33 0.7353669030165264 1106 34-35 0.4779063847695113 1106 36-37 0.47601216792453727 1106 38-39 0.8059118578281357 1106 40-41 0.7795981548420983 1106 42-43 0.45453143271195273 1106 44-45 0.9182642901264231 1106 46-47 0.4310825462710497 1106 48-49 0.7980200338332466 1106 50-51 0.7486802385026223 1106 52-53 1.17203556802815 1106 54-55 0.37475185583309667 1106 56-57 0.5966683585703265 1106 58-59 0.6877074186504473 1106 60-61 0.6845134632590657 1106 62-63 0.7446263862387212 1106 64-65 1.1555143481429795 1106 66-67 0.4979625525672233 1106 68-69 0.8560921298707314 1106 70-71 0.7261101017248208 1106 72-73 0.9996746192750194 1106 74-75 0.031142270314660436 1106 76 0.5990913019727238 1107 1 -0.5135654829086462 1107 2 -0.6970621621176463 1107 3 -0.8068926471711109 1107 4 -0.5552581993586116 1107 5 -0.7702002938209773 1107 6 -0.8064256199269089 1107 7 -0.6436899228218849 1107 8 -0.5675927592274732 1107 9 -0.09515580750262131 1107 10-11 -0.8706371225124343 1107 12-13 -0.5792711019898604 1107 14-15 -0.6423491511568216 1107 16-17 0.0015093337617173574 1107 18-19 -0.30436855639305804 1107 20-21 -0.3703913842153028 1107 22-23 -0.4308682537251016 1107 24-25 0.06827349059022225 1107 26-27 -0.038562476368973364 1107 28-29 -0.014949483978092815 1107 30-31 -0.25202357623162897 1107 32-33 -0.2554587850568666 1107 34-35 -0.18111502195831974 1107 36-37 -0.17077682290114637 1107 38-39 -0.6268098547100962 1107 40-41 -0.047618970540156624 1107 42-43 -0.29317498930639374 1107 44-45 -0.399778523329239 1107 46-47 -0.05515598583904335 1107 48-49 -0.2600839417019287 1107 50-51 -0.2941332171548723 1107 52-53 -0.31420296408194304 1107 54-55 -0.14665487199565064 1107 56-57 -0.2458392866896162 1107 58-59 -0.4529653642241769 1107 60-61 -0.5295538150283932 1107 62-63 -0.4893185678897112 1107 64-65 -0.6166569056796547 1107 66-67 -0.20693041379363564 1107 68-69 -0.07204242670419347 1107 70-71 0.11601835860555809 1107 72-73 0.32689174465728144 1107 74-75 0.08007193392322876 1107 76 0.07921362613174665 1108 1 -0.19781875407724314 1108 2 -0.3737754523367265 1108 3 0.6462281228694948 1108 4 0.19854939075518985 1108 5 0.30631246498986897 1108 6 0.7486252649179335 1108 7 0.49785517167854465 1108 8 0.36092782030829795 1108 9 -0.104520624267046 1108 10-11 0.22271024801947448 1108 12-13 0.04186992172297721 1108 14-15 0.27606965868927347 1108 16-17 0.14801256733067447 1108 18-19 -8.291770377653052E-4 1108 20-21 0.4940894901016435 1108 22-23 0.7225833931390895 1108 24-25 -0.11095145551696106 1108 26-27 0.3400809286543378 1108 28-29 0.5743621919631465 1108 30-31 0.5593164749038806 1108 32-33 0.29682977963958024 1108 34-35 0.2602796739859343 1108 36-37 0.049481862615472494 1108 38-39 -0.014181389276693324 1108 40-41 0.4885869752160943 1108 42-43 0.902729156680472 1108 44-45 0.35517710909669375 1108 46-47 0.39789961149389086 1108 48-49 0.1285709938773607 1108 50-51 0.19912892752854106 1108 52-53 8.489875457229346E-5 1108 54-55 0.6285845123729246 1108 56-57 0.5336914698151247 1108 58-59 0.31030229507296525 1108 60-61 0.40690279498814164 1108 62-63 0.48237141055315647 1108 64-65 0.6406950770746462 1108 66-67 0.6091923114282096 1108 68-69 0.41778561761325506 1108 70-71 0.12986505234395906 1108 72-73 -0.3346613710994717 1108 74-75 0.4572564729516557 1108 76 -0.025112839000858145 1109 1 0.1651020712834672 1109 2 -0.045782485485609925 1109 3 0.46784471282657236 1109 4 0.14263585711502103 1109 5 0.33438300333604687 1109 6 0.2593766741334207 1109 7 0.14778018589998254 1109 8 -0.2638950581113093 1109 9 0.33866084131707197 1109 10-11 -0.27217003330648026 1109 12-13 -0.34877495597262964 1109 14-15 -0.2667860066155825 1109 16-17 0.09827372469738549 1109 18-19 0.461590019321541 1109 20-21 0.2011122961211882 1109 22-23 0.48754693453641096 1109 24-25 -0.25301072013919423 1109 26-27 -0.10540182461659242 1109 28-29 -0.24417222392349203 1109 30-31 0.28089686482992704 1109 32-33 -0.03600364850336035 1109 34-35 0.09681067672678978 1109 36-37 -0.071581384010166 1109 38-39 0.0837603803876803 1109 40-41 -0.14707005534707918 1109 42-43 0.07340196011430322 1109 44-45 0.272377295268285 1109 46-47 0.25955860720160473 1109 48-49 0.38078178148660413 1109 50-51 0.05996049395559311 1109 52-53 0.08923523231200647 1109 54-55 0.48920405004681555 1109 56-57 0.06912612875911606 1109 58-59 0.1337806009115141 1109 60-61 0.4939897267769453 1109 62-63 0.5104884917963659 1109 64-65 0.14659568213230756 1109 66-67 0.07973065760393183 1109 68-69 -0.05612967431414262 1109 70-71 0.5239954758843197 1109 72-73 0.27688643879864827 1109 74-75 0.40992751809825734 1109 76 -0.16572410238194024 1110 1 -0.030501553973156348 1110 2 0.03991177118656353 1110 3 -0.04781299084374879 1110 4 0.348484337411449 1110 5 0.046255218047377866 1110 6 0.8425622932811905 1110 7 0.07464413863144159 1110 8 0.35598376618391825 1110 9 1.014761186818049 1110 10-11 0.546051387754062 1110 12-13 0.6838423391052331 1110 14-15 0.6467436113304359 1110 16-17 0.8475702045932323 1110 18-19 0.3568080855175282 1110 20-21 0.19786258403867407 1110 22-23 0.3855290085437204 1110 24-25 0.1863163040458744 1110 26-27 0.2969115297472342 1110 28-29 -0.0676362467565994 1110 30-31 -0.11544282772427295 1110 32-33 0.3346387830223563 1110 34-35 0.21260858369189606 1110 36-37 0.11885474838179988 1110 38-39 -0.1185529739761435 1110 40-41 0.10974232260095107 1110 42-43 0.1942722219940336 1110 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-0.07885896436433626 2116 9 -0.9784319186450006 2116 10-11 -0.12210520003443293 2116 12-13 -0.45839156877284637 2116 14-15 -0.1323634098011226 2116 16-17 -0.465001613500057 2116 18-19 -0.32206522249688163 2116 20-21 -0.6290825907592676 2116 22-23 -0.2789855498358662 2116 24-25 -0.7844403421708961 2116 26-27 -0.15993918599918544 2116 28-29 -0.6728217565433354 2116 30-31 0.4129873991722093 2116 32-33 -0.11464060153310385 2116 34-35 -0.0524026150616379 2116 36-37 -0.5039225424936475 2116 38-39 -0.2129292520947601 2116 40-41 -0.6548355330812043 2116 42-43 -0.7704127522001514 2116 44-45 -0.23319159250242194 2116 46-47 -0.3217926343071724 2116 48-49 -0.8034414772078264 2116 50-51 -0.130867049540754 2116 52-53 -0.448285814309898 2116 54-55 -0.501235461478025 2116 56-57 -0.4750282324880004 2116 58-59 -0.14882472896404408 2116 60-61 -0.3037982006872042 2116 62-63 -0.5123434642331262 2116 64-65 -0.9777148595641947 2116 66-67 -0.6775219599184723 2116 68-69 -0.21267299648712168 2116 70-71 -0.09016914256703501 2116 72-73 -0.5045044768019764 2116 74-75 -0.3103991641799979 2116 76 -0.25748737384949294 2117 1 0.2756046452926526 2117 2 -0.13709045206973158 2117 3 0.327255516472313 2117 4 0.237786855378463 2117 5 0.7096231928160037 2117 6 1.0232768375136487 2117 7 1.0494437870467266 2117 8 0.44395233037397475 2117 9 0.20139062686768483 2117 10-11 0.8571418174112608 2117 12-13 0.5026004177981704 2117 14-15 0.6593024242149781 2117 16-17 0.6671400303519803 2117 18-19 0.5526924761901952 2117 20-21 0.3961667048183557 2117 22-23 0.934849105746558 2117 24-25 0.7842533290187319 2117 26-27 1.2940885112890328 2117 28-29 0.7494569748968871 2117 30-31 1.1710108199671652 2117 32-33 1.03488179010575 2117 34-35 1.0377934241861766 2117 36-37 0.8777245069258299 2117 38-39 0.6077228132116943 2117 40-41 0.7176840009009311 2117 42-43 0.9003422287155018 2117 44-45 0.6169862616571677 2117 46-47 1.019857212818188 2117 48-49 0.622029636478949 2117 50-51 0.8442883230537177 2117 52-53 0.9521808030638539 2117 54-55 1.0264218595187558 2117 56-57 0.9762717015660769 2117 58-59 0.9981647396716724 2117 60-61 0.6998488087650472 2117 62-63 0.6747780960874152 2117 64-65 0.7853044645900056 2117 66-67 0.7010276294182702 2117 68-69 0.8337839088504992 2117 70-71 1.10187338256236 2117 72-73 -0.5451061834025666 2117 74-75 0.9094805344671073 2117 76 0.159700532067653 2118 1 -0.06304123651283788 2118 2 -0.04262791135311872 2118 3 -0.12775527078602877 2118 4 -0.4246036856765727 2118 5 0.009963731755892269 2118 6 0.28101828173717536 2118 7 0.5887855527728618 2118 8 -0.12208261188246183 2118 9 0.6190901911470519 2118 10-11 -1.021913980211309 2118 12-13 -0.2830768528139558 2118 14-15 -0.13958394999712453 2118 16-17 0.11816905019207269 2118 18-19 0.5556536843631292 2118 20-21 0.37690298807906686 2118 22-23 0.47744820046290926 2118 24-25 0.5221388148683843 2118 26-27 0.12277733061303309 2118 28-29 0.4465412424208921 2118 30-31 0.3164114291299853 2118 32-33 0.41573546411903806 2118 34-35 0.03721175829507217 2118 36-37 0.13764984217689857 2118 38-39 0.16239940697622757 2118 40-41 0.20692846978709412 2118 42-43 0.45516688288869034 2118 44-45 0.3139859232333748 2118 46-47 0.14923557061673165 2118 48-49 0.2738232825722733 2118 50-51 0.46973250427965496 2118 52-53 -0.06747950405573278 2118 54-55 -0.23195412931325876 2118 56-57 0.11586093968951161 2118 58-59 -0.11092934214227768 2118 60-61 0.09002500065133745 2118 62-63 0.2359534842171982 2118 64-65 0.060638658083796315 2118 66-67 0.05169284368283655 2118 68-69 -0.20699615798820048 2118 70-71 -0.04672638900157722 2118 72-73 0.12017682349281955 2118 74-75 0.2713771883018765 2118 76 -0.04587076959485259 2119 1 0.19560298550717903 2119 2 -0.28785084128942273 2119 3 -0.40842364699580713 2119 4 0.09092696201967954 2119 5 0.06801237101472069 2119 6 0.05095376027138343 2119 7 0.19855476445290066 2119 8 0.4395850198488631 2119 9 -0.42739835215677857 2119 10-11 -0.32675722613736013 2119 12-13 0.05858705652510565 2119 14-15 0.08796795669490365 2119 16-17 -0.021017400300110012 2119 18-19 -0.26296241291539246 2119 20-21 0.017082076506568455 2119 22-23 -0.5831647534621425 2119 24-25 -0.28459664712185173 2119 26-27 -0.11306723883025427 2119 28-29 0.11701646783242126 2119 30-31 -0.5758563349530732 2119 32-33 0.019342048617332352 2119 34-35 -0.49327049830183967 2119 36-37 -0.04457159880250572 2119 38-39 -0.4547525493476954 2119 40-41 -0.37368200254312 2119 42-43 -0.5432821203373877 2119 44-45 -0.2692198836985469 2119 46-47 0.15660797528213521 2119 48-49 0.23912231475028278 2119 50-51 -0.09852169375160713 2119 52-53 -0.1047861464579114 2119 54-55 -0.28108970711125636 2119 56-57 -0.4203703449494185 2119 58-59 -0.4292455800445012 2119 60-61 -0.004864981977505067 2119 62-63 -0.3526291115310016 2119 64-65 -0.5375601176627427 2119 66-67 -0.7717933834658126 2119 68-69 -0.8395340023094775 2119 70-71 -0.5936078514881373 2119 72-73 -0.5216980214075022 2119 74-75 -0.5610634602215754 2119 76 -0.30594190864688287 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 27.0 13 255.0 14 1124.0 15 2053.0 16 2568.0 17 3030.0 18 3122.0 19 2635.0 20 2117.0 21 1557.0 22 1140.0 23 1038.0 24 928.0 25 858.0 26 1041.0 27 1218.0 28 1427.0 29 1739.0 30 2017.0 31 2506.0 32 3145.0 33 4223.0 34 5716.0 35 8493.0 36 15893.0 37 50162.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.42054268409521 23.138636874859415 11.271900227437454 28.168920213607922 2 20.06048387096774 19.58227805918422 30.27525993068515 30.081978139162892 3 20.6726483550357 20.843434722118083 26.84928311381037 31.63463380903585 4 15.866470054068465 18.071696950005414 30.528271392033858 35.53356160389226 5 17.601826164471436 30.7590412636525 30.714053635250306 20.92507893662576 6 30.35331950380312 29.53104562911866 16.595436255029867 23.520198612048354 7 28.07811185257388 24.30248348370865 21.564069880782785 26.055334782934693 8 24.84233502453492 28.58713853690235 18.533236693242692 28.037289745320038 9 23.964243166462555 16.213041413611258 19.354677463697485 40.468037956228706 10-11 26.94509010022244 21.685286546199794 23.7926236951505 27.576999658427265 12-13 25.678771671123773 19.259703581515083 23.630584922479652 31.43093982488149 14-15 25.178076029092 20.37481359292861 21.625303041663543 32.82180733631584 16-17 26.229453566935764 22.14516008097773 22.05268551148434 29.572700840602167 18-19 25.530062566127647 21.190422633775714 23.00575675022702 30.27375804986962 20-21 24.83317087800855 21.81525080602834 23.344413619587947 30.007164696375167 22-23 27.071305391017468 21.585314038639375 22.023110311331052 29.320270259012105 24-25 25.703871868035737 22.693853747943265 21.560410722096098 30.041863661924893 26-27 24.886176660487784 22.976277258242558 23.2803615687418 28.857184512527855 28-29 25.59421159181225 22.248048453341998 24.37329734323061 27.78444261161514 30-31 25.594628143927086 21.519082252380596 24.179184057717464 28.70710554597486 32-33 24.45994018311631 22.755408929211132 24.40578840818775 28.378862479484805 34-35 24.751110111386033 22.54046803795623 24.13253022085593 28.575891629801802 36-37 25.25857580238685 21.433361797846416 24.417553579238955 28.89050882052777 38-39 23.83844442778236 21.265818566560863 26.780135462747744 28.115601542909037 40-41 23.681820832604366 21.08545150083727 26.669332600201614 28.56339506635675 42-43 22.69042679929686 21.023801787841677 26.559362841885147 29.726408570976314 44-45 21.853573600593172 20.958819657927403 28.137262252880458 29.050344488598967 46-47 21.60947406129981 22.02644272824973 28.212658185665607 28.15142502478485 48-49 21.623636833204202 22.25054776603101 27.286662834387208 28.839152566377578 50-51 21.283313755383936 23.43063990735881 26.289853623586843 28.99619271367041 52-53 20.480617830096723 23.154882407337983 26.94592320445211 29.418576558113184 54-55 20.462706089158814 22.812893121058377 26.809710662901036 29.914690126881776 56-57 21.008389359592776 23.07448784917481 26.316512958936293 29.600609832296122 58-59 21.409112494064132 22.917447701881983 25.592961935467745 30.080477868586136 60-61 20.95465413677905 22.298867811351876 26.032007864503925 30.71447018736514 62-63 21.27706547366141 22.25054776603101 25.834978714186935 30.63740804612065 64-65 21.952296451809087 22.63710812859797 24.83442053435305 30.576174885239894 66-67 21.45627225959636 23.431570616291424 23.9197717284902 31.19238539562202 68-69 21.434194905546413 24.542519734238645 23.84770791244038 30.17557744777456 70-71 21.335382769738324 24.94147442786567 24.29373588929711 29.429406913098898 72-73 21.54324227504103 24.24708205243558 25.4000983062991 28.809577366224286 74-75 20.94132446910433 25.62003782293203 25.00520690143544 28.433430806528204 76 20.648488332375265 25.251389201302977 23.94008314380212 30.160039322519637 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 1.0 14 2.5 15 4.0 16 6.0 17 7.0 18 8.5 19 18.0 20 22.0 21 18.0 22 35.5 23 63.0 24 84.5 25 96.0 26 151.5 27 259.0 28 371.0 29 431.0 30 526.0 31 733.0 32 1062.0 33 1279.0 34 1406.5 35 1825.0 36 2271.0 37 2426.0 38 2728.5 39 3495.5 40 4258.5 41 4809.0 42 5061.0 43 5158.0 44 5537.5 45 5853.5 46 5887.0 47 5863.0 48 5671.5 49 5187.0 50 4870.0 51 4528.0 52 3834.0 53 3369.5 54 3257.0 55 3185.0 56 3018.5 57 2986.0 58 3048.0 59 3008.0 60 3003.5 61 3063.0 62 3087.0 63 3077.5 64 3180.0 65 3302.0 66 3324.5 67 3337.0 68 3426.5 69 3317.0 70 3012.0 71 2906.0 72 2765.0 73 2420.5 74 1892.5 75 1568.0 76 1289.5 77 803.0 78 438.5 79 282.0 80 192.5 81 62.5 82 20.5 83 19.0 84 14.5 85 9.0 86 8.5 87 9.0 88 7.0 89 5.0 90 4.5 91 2.0 92 0.0 93 0.5 94 2.5 95 5.0 96 6.0 97 6.5 98 5.0 99 16.0 100 29.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 8.33104229670174E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.16552114835087E-4 26-27 4.16552114835087E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 4.16552114835087E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.16552114835087E-4 68-69 4.16552114835087E-4 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 120033.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.47505269384253 #Duplication Level Percentage of deduplicated Percentage of total 1 76.78589332263887 51.043463047661895 2 13.179266091838782 17.5218481584231 3 4.733557248847002 9.439904026392743 4 1.9688690595548426 5.235226979247374 5 1.040204531782635 3.4573825531312226 6 0.6379085622618809 2.5443003174127115 7 0.46370563465009024 2.1577399548457508 8 0.34965911369560854 1.8594886406238285 9 0.19926809705233606 1.1921721526580191 >10 0.6416683376779627 5.548474169603359 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004998625378021045 0.0 2 0.0 0.0 0.0 0.004998625378021045 0.0 3 0.0 0.0 0.0 0.005831729607691218 0.0 4 0.0 0.0 0.0 0.02832554380878592 0.0 5 0.0 0.0 0.0 0.035823481875817485 0.0 6 0.0 0.0 0.0 0.035823481875817485 0.0 7 0.0 0.0 0.0 0.035823481875817485 0.0 8 0.0 0.0 0.0 0.03748969033515783 0.0 9 0.0 0.0 0.0 0.045820732631859575 0.0 10 0.0 0.0 0.0 0.04748694109119992 0.0 11 0.0 0.0 0.0 0.04748694109119992 0.0 12 0.0 0.0 0.0 0.04748694109119992 0.0 13 0.0 0.0 0.0 0.05165246223955079 0.0 14 0.0 0.0 0.0 0.052485566469220965 0.0 15 0.0 0.0 0.0 0.054984879158231484 0.0 16 0.0 0.0 0.0 0.054984879158231484 0.0 17 0.0 0.0 0.0 0.05748419184724201 0.0 18 0.0 0.0 0.0 0.06331592145493323 0.0 19 0.0 0.0 0.0 0.06914765106262445 0.0 20 0.0 0.0 0.0 0.07331317221097532 0.0 21 0.0 0.0 0.0 0.07581248489998584 0.0 22 0.0 0.0 0.0 0.08497663142635775 0.0 23 0.0 0.0 0.0 0.0933076737230595 0.0 24 0.0 0.0 0.0 0.0991394033307507 0.0 25 0.0 0.0 0.0 0.10830354985712262 0.0 26 0.0 0.0 0.0 0.12329942599118576 0.0 27 0.0 0.0 0.0 0.21993951663292594 0.0 28 0.0 0.0 0.0 0.35240308915048363 0.0 29 0.0 0.0 0.0 0.47320320245265884 0.0 30 0.0 0.0 0.0 0.617330234185599 0.0 31 0.0 0.0 0.0 0.789782809727325 0.0 32 0.0 0.0 0.0 0.9705664275657527 0.0 33 0.0 0.0 0.0 1.1946714653470296 0.0 34 0.0 0.0 0.0 1.5379104079711412 0.0 35 0.0 0.0 0.0 1.9886197962227055 0.0 36 0.0 0.0 0.0 2.623445219231378 0.0 37 0.0 0.0 0.0 3.441553572767489 0.0 38 0.0 0.0 0.0 4.222172235968442 0.0 39 0.0 0.0 0.0 5.042779902193564 0.0 40 0.0 0.0 0.0 5.8850482783901095 0.0 41 0.0 0.0 0.0 6.729815967275666 0.0 42 0.0 0.0 0.0 7.61040713803704 0.0 43 0.0 0.0 0.0 8.52265626952588 0.0 44 0.0 0.0 0.0 9.385752251464181 0.0 45 0.0 0.0 0.0 10.263844109536544 0.0 46 0.0 0.0 0.0 11.15359942682429 0.0 47 0.0 0.0 0.0 12.01669540876259 0.0 48 0.0 0.0 0.0 12.82480651154266 0.0 49 0.0 0.0 0.0 13.619587946648005 0.0 50 0.0 0.0 0.0 14.346054834920396 0.0 51 0.0 0.0 0.0 15.038364449776312 0.0 52 0.0 0.0 0.0 15.765664442278373 0.0 53 0.0 0.0 0.0 16.498796164388125 0.0 54 0.0 0.0 0.0 17.14695125507152 0.0 55 0.0 0.0 0.0 17.85842226720985 0.0 56 0.0 0.0 0.0 18.52657185940533 0.0 57 0.0 0.0 0.0 19.1364041555239 0.0 58 0.0 0.0 0.0 19.7620654320062 0.0 59 0.0 0.0 0.0 20.38189497888081 0.0 60 0.0 0.0 0.0 21.01172177651146 0.0 61 0.0 0.0 0.0 21.614889238792664 0.0 62 0.0 0.0 0.0 22.26054501678705 0.0 63 0.0 0.0 0.0 22.908700107470445 0.0 64 0.0 0.0 0.0 23.524364133196705 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTGGAA 15 0.0022200386 70.0 46 CGTGGTA 20 0.0069369497 52.5 29 TATGGAC 20 0.0069369497 52.5 21 GTCGTAA 20 0.0069369497 52.5 1 CATACGC 20 0.0069369497 52.5 23 ATGGTTC 20 0.0069369497 52.5 12 TGCTACA 20 0.0069369497 52.5 43 GTAGCGT 35 0.0012512805 40.0 25 GTTTTAC 35 0.0012512805 40.0 35 AGGTGGG 40 0.0024108381 35.0 15 GAGAAGT 55 2.977618E-4 31.81818 47 GTCTTAG 45 0.004293249 31.111113 30 ACAGACA 50 0.007185065 28.000002 2 AGGGGTA 50 0.007185065 28.000002 12 CTCACAA 70 0.0012201624 25.0 30 TGGTTCT 70 0.0012201624 25.0 13 TATACCC 70 0.0012201624 25.0 3 ACTTTAC 75 0.0018235256 23.333334 52 AGAAGTA 80 0.0026533862 21.875 48 GGGAGAG 80 0.0026533862 21.875 36 >>END_MODULE