FastQCFastQC Report
Wed 25 May 2016
SRR1780280_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780280_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120033
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT91247.601242991510667No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC26182.1810668732765155No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG23981.9977839427490773No Hit
CACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCTG21731.810335491073288TruSeq Adapter, Index 2 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC5700.4748694109119992No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT2780.23160297584830838TruSeq Adapter, Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT2490.20744295318787334No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT1810.1507918655703015No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG1280.10663734139778228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACT308.334635E-658.33333234
TAACTCG308.334635E-658.33333236
AGTCATG252.3823878E-456.00000437
GTTGAAA200.006936949752.570
ATAATAC200.006936949752.54
TGTCTGA200.006936949752.548
ACGTAAC352.0762105E-550.033
CACGTAA352.0762105E-550.032
GTAACTC352.0762105E-550.035
TCTTTAT751.4551915E-1146.6666681
CTTTATA802.910383E-1143.752
TTTATAC1007.2759576E-1238.5000043
ACTCGTA556.6492194E-638.18181638
AAACAAT501.7013882E-435.00000468
CTTGAAA19850.034.73551657
TCGTATG20600.034.66019440
CTCGTAT20400.034.65686439
GTATGCC20550.034.57420742
TATGCCG20450.034.5721343
CTGCTTG20050.034.5635954