##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780280_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 120033 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.76422317196104 34.0 34.0 34.0 34.0 34.0 2 33.73956328676281 34.0 34.0 34.0 34.0 34.0 3 33.754075962443665 34.0 34.0 34.0 34.0 34.0 4 33.767005740088145 34.0 34.0 34.0 34.0 34.0 5 33.75774162105421 34.0 34.0 34.0 34.0 34.0 6 37.514133613256355 38.0 38.0 38.0 37.0 38.0 7 37.50631909558205 38.0 38.0 38.0 37.0 38.0 8 37.4241083701982 38.0 38.0 38.0 37.0 38.0 9 37.443836278356784 38.0 38.0 38.0 37.0 38.0 10-11 37.38484833337499 38.0 38.0 38.0 37.0 38.0 12-13 37.3716769555039 38.0 38.0 38.0 37.0 38.0 14-15 37.29406913098897 38.0 38.0 38.0 36.0 38.0 16-17 37.33227529096165 38.0 38.0 38.0 36.5 38.0 18-19 37.28052702173569 38.0 38.0 38.0 36.0 38.0 20-21 37.29544791848909 38.0 38.0 38.0 36.0 38.0 22-23 37.2955270633909 38.0 38.0 38.0 36.0 38.0 24-25 37.27954812426583 38.0 38.0 38.0 36.0 38.0 26-27 37.17385219064757 38.0 38.0 38.0 36.0 38.0 28-29 37.16374247082052 38.0 38.0 38.0 36.0 38.0 30-31 37.1016595436255 38.0 38.0 38.0 36.0 38.0 32-33 37.02237717960894 38.0 38.0 38.0 36.0 38.0 34-35 37.11140686311264 38.0 38.0 38.0 36.0 38.0 36-37 37.029716827872335 38.0 38.0 38.0 36.0 38.0 38-39 36.42225471328718 38.0 37.0 38.0 34.0 38.0 40-41 36.75991602309365 38.0 37.0 38.0 34.5 38.0 42-43 37.02087342647439 38.0 38.0 38.0 36.0 38.0 44-45 37.088525655444755 38.0 38.0 38.0 36.0 38.0 46-47 37.036340006498214 38.0 38.0 38.0 36.0 38.0 48-49 37.0559471145435 38.0 38.0 38.0 36.0 38.0 50-51 36.86995242974849 38.0 38.0 38.0 35.0 38.0 52-53 36.873547274499515 38.0 38.0 38.0 35.0 38.0 54-55 36.84107287162697 38.0 37.5 38.0 35.0 38.0 56-57 36.89916939508302 38.0 38.0 38.0 35.0 38.0 58-59 36.79171144601901 38.0 37.5 38.0 35.0 38.0 60-61 36.79104912815642 38.0 37.0 38.0 35.0 38.0 62-63 36.77629068672782 38.0 37.0 38.0 35.0 38.0 64-65 36.706830621579066 38.0 37.5 38.0 34.5 38.0 66-67 36.35445252555547 38.0 37.0 38.0 34.0 38.0 68-69 36.252913782043265 38.0 37.0 38.0 34.0 38.0 70-71 35.48446260611665 38.0 37.0 38.0 29.0 38.0 72-73 34.14953804370465 38.0 36.0 38.0 22.0 38.0 74-75 33.73602675930786 38.0 35.5 38.0 19.0 38.0 76 33.06243283097148 38.0 34.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0816065089903546 1101 2 0.05341142778127761 1101 3 0.05632694298003571 1101 4 0.020555733405856813 1101 5 -0.14777238229157774 1101 6 0.06617516359706599 1101 7 -0.007012291871689058 1101 8 0.09910276786825989 1101 9 -6.954265476863952E-4 1101 10-11 0.07649391912207193 1101 12-13 -0.03944723211165524 1101 14-15 0.20715951915600783 1101 16-17 0.19697396266956702 1101 18-19 0.19220735865530258 1101 20-21 0.02012167849788682 1101 22-23 0.12446863093518346 1101 24-25 -0.005113847822009632 1101 26-27 0.07427060055393753 1101 28-29 -0.1715093504850458 1101 30-31 -0.03167615581985217 1101 32-33 -0.06748342248071282 1101 34-35 -0.05687147945381099 1101 36-37 0.06790312953027211 1101 38-39 0.4516691642717774 1101 40-41 0.1566926966744262 1101 42-43 0.1388094049937223 1101 44-45 0.14220892097200277 1101 46-47 0.01655211497244835 1101 48-49 -0.08464553778814832 1101 50-51 0.07542949439670821 1101 52-53 -0.2164076060705895 1101 54-55 -0.14182222511926312 1101 56-57 -0.1174834925733208 1101 58-59 -0.09185666499097067 1101 60-61 -0.09358400451613846 1101 62-63 -0.09048503507710848 1101 64-65 -0.20022241041117894 1101 66-67 -0.20250766397609965 1101 68-69 -0.13063895906139322 1101 70-71 0.4472236042772195 1101 72-73 0.1439922928300419 1101 74-75 0.6645944600656364 1101 76 -0.38924785885025415 1102 1 -0.03908992014788737 1102 2 -0.07057480962348706 1102 3 -0.07723371893526121 1102 4 0.09472424327724127 1102 5 -0.1274152101772188 1102 6 -0.009191429341413482 1102 7 0.013600880898039236 1102 8 -0.10397743201704657 1102 9 -0.06285238566950113 1102 10-11 0.049403929771699495 1102 12-13 0.18308901758114615 1102 14-15 0.15609250842445022 1102 16-17 0.1122223857055289 1102 18-19 0.04533466266120456 1102 20-21 -0.0400999156694013 1102 22-23 -0.01908629656543326 1102 24-25 0.07557755874799454 1102 26-27 0.17741838461225967 1102 28-29 0.14320761519039138 1102 30-31 0.17580213532457378 1102 32-33 0.1833869797475174 1102 34-35 0.06919860328560645 1102 36-37 0.05792422398427277 1102 38-39 0.19008769462321595 1102 40-41 0.31979071420535377 1102 42-43 0.19741307501911365 1102 44-45 0.055255738371855045 1102 46-47 -0.07781484660697657 1102 48-49 0.0911808323273604 1102 50-51 0.015008219718239957 1102 52-53 0.2017285043578596 1102 54-55 0.1290725483713544 1102 56-57 0.30821487721451035 1102 58-59 0.13281463016754458 1102 60-61 0.44900938452294525 1102 62-63 0.34017462741162063 1102 64-65 0.3588727808725096 1102 66-67 0.047825136875871976 1102 68-69 0.22343042735623442 1102 70-71 0.017664744606534555 1102 72-73 0.19860747264490186 1102 74-75 0.5752399913181989 1102 76 0.30496447833333207 1103 1 -0.01453920129804942 1103 2 -0.07615587991102757 1103 3 0.03933976349285473 1103 4 -0.10845067685055199 1103 5 0.07402248950329238 1103 6 -0.1579434261465238 1103 7 0.016785785051389723 1103 8 -0.02124979623430079 1103 9 -0.24019863167588795 1103 10-11 -0.08079774754458668 1103 12-13 -0.03872607826550478 1103 14-15 0.020060160181643027 1103 16-17 -0.0630821270740185 1103 18-19 0.05386601250145873 1103 20-21 0.025450204138905974 1103 22-23 -0.017598676757124565 1103 24-25 -0.05651609141175129 1103 26-27 -0.017035489189652253 1103 28-29 0.0317759059252154 1103 30-31 0.021528972385269185 1103 32-33 0.09088997495518214 1103 34-35 -0.017248082017914612 1103 36-37 7.556936328327879E-4 1103 38-39 0.07041916427178307 1103 40-41 0.2063721838539152 1103 42-43 -0.2901168770575637 1103 44-45 -0.2475747328741491 1103 46-47 -0.2906193593865325 1103 48-49 -0.015054191634298775 1103 50-51 -0.34892947996227264 1103 52-53 -0.17886754196801746 1103 54-55 -0.17207062255516092 1103 56-57 -0.40201874898357914 1103 58-59 -0.265053780375581 1103 60-61 -0.2899782352853677 1103 62-63 -0.22682317610274794 1103 64-65 0.004384961383699704 1103 66-67 -0.2420909973094325 1103 68-69 -0.11629601034344716 1103 70-71 0.014050527354136477 1103 72-73 -0.44426892511867067 1103 74-75 0.0013332421169209852 1103 76 0.04296367961128311 1104 1 0.05729598978475536 1104 2 0.05782103091699753 1104 3 -0.03796915370460141 1104 4 -0.06406373417539157 1104 5 0.007461979949148656 1104 6 0.11337424901270765 1104 7 -0.07101278182759074 1104 8 -0.05735648413239147 1104 9 0.16838018998016224 1104 10-11 0.09414253907960557 1104 12-13 0.08095943128864036 1104 14-15 0.04165649776125946 1104 16-17 -0.010205932806506723 1104 18-19 0.09048026409381293 1104 20-21 0.04337416910706082 1104 22-23 -0.16809628822208822 1104 24-25 -0.15953161688946693 1104 26-27 0.152520641383596 1104 28-29 0.018021925033487207 1104 30-31 0.16062132907316595 1104 32-33 -0.0036462511594592684 1104 34-35 0.15943384473367672 1104 36-37 0.055970661785700315 1104 38-39 0.21520419611891128 1104 40-41 0.16974798003225544 1104 42-43 0.008828186636705482 1104 44-45 -0.14738564051746295 1104 46-47 -0.20979730524640416 1104 48-49 -0.0032209909973559547 1104 50-51 -0.2584040022552685 1104 52-53 -0.026110774452092755 1104 54-55 -0.10971990599465187 1104 56-57 -0.3850402457988622 1104 58-59 0.04076827376454162 1104 60-61 -0.15994240725988362 1104 62-63 -0.1952845614530716 1104 64-65 -0.2623447360685347 1104 66-67 -0.6254110610037031 1104 68-69 -0.4263477619358014 1104 70-71 -0.44737463188153015 1104 72-73 -0.4493545142906541 1104 74-75 -0.2523988438703384 1104 76 -0.8523786770766151 1105 1 0.0273280347267999 1105 2 0.1183899275423883 1105 3 0.1089826206357074 1105 4 -0.06904850293750542 1105 5 0.03458149571446967 1105 6 0.06649368565472003 1105 7 -0.055089214948608856 1105 8 -0.07058985834610354 1105 9 -0.11110313478148015 1105 10-11 -0.07037461089863228 1105 12-13 0.1283740770140014 1105 14-15 0.1377814334735703 1105 16-17 0.2054446586402605 1105 18-19 0.14651935101076674 1105 20-21 0.15212916376623298 1105 22-23 -0.006981440732275246 1105 24-25 -0.12137439886517143 1105 26-27 -0.03954131217101775 1105 28-29 0.007261154372415035 1105 30-31 -0.4714058102234233 1105 32-33 -0.35405373622494096 1105 34-35 -0.16060017829120454 1105 36-37 -0.11760610760940438 1105 38-39 -0.27270971771579866 1105 40-41 0.07127901615204735 1105 42-43 0.10598172542690776 1105 44-45 -0.08190120802943568 1105 46-47 0.099258358004775 1105 48-49 -0.10999790281442756 1105 50-51 -0.35972140542811104 1105 52-53 0.009573840019555746 1105 54-55 -0.15300035858000882 1105 56-57 -0.22793101606431776 1105 58-59 0.16659412335112478 1105 60-61 0.15740142309972782 1105 62-63 -0.3747560177176581 1105 64-65 -0.13821014731195902 1105 66-67 -0.5082788855081901 1105 68-69 -0.28052349481549754 1105 70-71 -0.4363764912794039 1105 72-73 -0.20245021393854756 1105 74-75 -0.2847207175104103 1105 76 -0.3337055750470981 1106 1 0.10681974365608227 1106 2 -0.09373042119543129 1106 3 0.03165627725432074 1106 4 0.06404550051947666 1106 5 0.12004695433509482 1106 6 0.2226089438840546 1106 7 0.047357268231820626 1106 8 -0.006284199904030174 1106 9 0.027356796458668953 1106 10-11 -0.052519842345816414 1106 12-13 -0.0721454207731469 1106 14-15 -0.07956713339633836 1106 16-17 0.10398113745197435 1106 18-19 0.07273546968799849 1106 20-21 -0.005078274454362486 1106 22-23 0.012877240674072254 1106 24-25 0.11673983366469542 1106 26-27 -0.17869928888384123 1106 28-29 0.08413637993132284 1106 30-31 -0.014518428226352853 1106 32-33 0.10327051776864238 1106 34-35 0.09419591645303882 1106 36-37 0.13410814011601246 1106 38-39 0.20816763521979453 1106 40-41 0.14215200036767328 1106 42-43 0.19888350520543696 1106 44-45 0.341764907798634 1106 46-47 0.18611515896209596 1106 48-49 0.1798951585186046 1106 50-51 0.31341761483895425 1106 52-53 0.22288608953044786 1106 54-55 0.10848652576287776 1106 56-57 0.4043937127901245 1106 58-59 0.1568642242115743 1106 60-61 0.1555779573751792 1106 62-63 -0.10899538558287247 1106 64-65 -0.018412248096424833 1106 66-67 -0.29941133370087414 1106 68-69 0.07992914256175254 1106 70-71 0.07481123071805484 1106 72-73 0.840990058061756 1106 74-75 0.42294543630652726 1106 76 0.4680210190608278 1107 1 -0.31519860496777596 1107 2 -0.1059628370975716 1107 3 -0.029505802256380775 1107 4 0.04875498064180306 1107 5 -0.048148764319336124 1107 6 0.07581995305837097 1107 7 0.10546124376698884 1107 8 0.07322650345988535 1107 9 0.06711214814062316 1107 10-11 -0.1427339096241056 1107 12-13 -0.030861017103426036 1107 14-15 0.009117881894184166 1107 16-17 -0.02598828150826904 1107 18-19 -0.22848777190220915 1107 20-21 0.0025669854844778683 1107 22-23 -0.1400279581026922 1107 24-25 0.006648090545432694 1107 26-27 0.12099490071860686 1107 28-29 0.016858092469547614 1107 30-31 0.034411235382215466 1107 32-33 0.042108438257933756 1107 34-35 -0.08164506214023248 1107 36-37 -0.16711510147420228 1107 38-39 -0.3101809886334195 1107 40-41 -0.04286329015219792 1107 42-43 0.11019848991491443 1107 44-45 0.04207071819618591 1107 46-47 -0.24507750158836217 1107 48-49 -0.19166447450891866 1107 50-51 0.08711792064934798 1107 52-53 -0.02787843646343191 1107 54-55 0.10542842178734446 1107 56-57 -0.4503463483719514 1107 58-59 -0.21469540881595606 1107 60-61 -0.4850948254994307 1107 62-63 -0.3979862112709469 1107 64-65 0.18648071826442703 1107 66-67 0.33361618923490255 1107 68-69 0.21295666549753633 1107 70-71 -0.00928662860916063 1107 72-73 -0.03209862695353394 1107 74-75 -0.08011266766901315 1107 76 -0.13136736014406836 1108 1 0.06543006099703064 1108 2 0.022768300416842635 1108 3 0.12724959955842507 1108 4 0.018905880526496333 1108 5 -0.028436526890153857 1108 6 -0.1891421966383291 1108 7 0.008845211280899434 1108 8 0.12516413819192707 1108 9 -0.21975805790540193 1108 10-11 0.05306495737344363 1108 12-13 -0.13969943892123382 1108 14-15 -0.01451975785114712 1108 16-17 0.0566156188276139 1108 18-19 -0.06091370061329826 1108 20-21 -0.12104057454961747 1108 22-23 0.11800583143959642 1108 24-25 -0.07657244387077355 1108 26-27 0.02346143703984893 1108 28-29 -0.011000733419052722 1108 30-31 0.05493061172953162 1108 32-33 -0.01717867258579986 1108 34-35 -0.028083123001515276 1108 36-37 -0.016150044072084313 1108 38-39 -0.09218329474461484 1108 40-41 -0.08707043909690526 1108 42-43 -0.052668106565761263 1108 44-45 0.04225620974879973 1108 46-47 -0.01211013807505168 1108 48-49 -0.08049988835561095 1108 50-51 -0.2151180045524299 1108 52-53 -0.19226405836145943 1108 54-55 -0.22880730288303397 1108 56-57 -0.08372981455734418 1108 58-59 -0.0713806246378823 1108 60-61 0.14962217455070004 1108 62-63 -0.031715594135540925 1108 64-65 -0.15752589927203786 1108 66-67 -0.21391476780124208 1108 68-69 -0.4479046169008214 1108 70-71 0.22644806833774567 1108 72-73 0.14510095193050887 1108 74-75 0.06385885687101478 1108 76 0.3156071222342334 1109 1 0.07151166620983673 1109 2 0.13530902545174683 1109 3 0.049947019013359295 1109 4 -0.2063410553994487 1109 5 0.047366338083733694 1109 6 -0.23019303182476136 1109 7 -0.11029426226721739 1109 8 -0.09276004860023335 1109 9 -0.05519271684939042 1109 10-11 -0.07932889896414963 1109 12-13 8.342529647862307E-4 1109 14-15 0.11750692674316099 1109 16-17 0.13029819626982686 1109 18-19 0.03493363868442145 1109 20-21 0.07139361896540919 1109 22-23 0.08136622860565268 1109 24-25 -0.0073786702761111655 1109 26-27 -0.011135449757482263 1109 28-29 -0.0010612391851978487 1109 30-31 0.014707205823754066 1109 32-33 0.20416383142205774 1109 34-35 0.033979244480512705 1109 36-37 0.2710041163457646 1109 38-39 -0.20566522058626902 1109 40-41 0.11331224694539799 1109 42-43 -0.015087302925067547 1109 44-45 0.18138327185771175 1109 46-47 0.07117579045574018 1109 48-49 0.026295374611756017 1109 50-51 -0.01624020551431471 1109 52-53 -0.08586182430240541 1109 54-55 -0.20507042539427545 1109 56-57 0.1986614560637463 1109 58-59 0.23499058082315116 1109 60-61 -0.006332730553502586 1109 62-63 -0.12208638588192855 1109 64-65 -0.15768621369516467 1109 66-67 -0.16853027175738333 1109 68-69 0.07793703382058936 1109 70-71 0.08205131599767412 1109 72-73 -0.3497845008284557 1109 74-75 -0.24341734936572834 1109 76 0.5175102095797399 1110 1 0.051482624098774465 1110 2 -0.05269357832372634 1110 3 -0.014826903173812411 1110 4 0.15593424378436538 1110 5 -0.08391401843321944 1110 6 -0.3965842991623987 1110 7 -0.22493048478987987 1110 8 0.09690496567046125 1110 9 0.02671605086379003 1110 10-11 -0.11854576341760747 1110 12-13 0.06201796935354764 1110 14-15 -0.26887833188185084 1110 16-17 -0.13185692866132825 1110 18-19 -0.19005759860965554 1110 20-21 -0.022888089511880594 1110 22-23 -0.22250939104283418 1110 24-25 0.1639948212866642 1110 26-27 0.23095558223892 1110 28-29 0.17299614402044483 1110 30-31 -0.08620912285282145 1110 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0.30157208952574166 2115 58-59 0.2116251792517403 2115 60-61 0.297152975894754 2115 62-63 0.37834957234445454 2115 64-65 0.09315631231537225 2115 66-67 0.26967142505081654 2115 68-69 0.2427684306503437 2115 70-71 -0.05283611860858883 2115 72-73 0.4326429537633132 2115 74-75 0.1702482265889742 2115 76 0.6259217541454447 2116 1 -0.148147330132403 2116 2 -0.18679621733434004 2116 3 -0.005828948163589587 2116 4 0.023642881431655383 2116 5 -0.1900879399445614 2116 6 -0.07878315007290837 2116 7 -0.15324872415106228 2116 8 0.016595295790239106 2116 9 -0.16748007339368343 2116 10-11 -0.05911446533600184 2116 12-13 -0.14480353225323483 2116 14-15 -0.09276576006375592 2116 16-17 -0.6385518356629802 2116 18-19 -0.2697440335108183 2116 20-21 -0.08690600751752697 2116 22-23 -0.08736478105160472 2116 24-25 -0.0370471496939615 2116 26-27 0.08958836050359764 2116 28-29 0.06342851328717103 2116 30-31 0.05780197852024571 2116 32-33 -0.1907530465172087 2116 34-35 -0.04785582741669003 2116 36-37 -0.3944513615818863 2116 38-39 -0.11658236947054945 2116 40-41 -0.18933333761847848 2116 42-43 -0.160879913867376 2116 44-45 -0.3471433678434721 2116 46-47 0.09779130012267245 2116 48-49 -0.006513164026934248 2116 50-51 -0.1907239584898761 2116 52-53 -0.16189093153857215 2116 54-55 -0.1247642575244825 2116 56-57 -0.07634773671363604 2116 58-59 -0.1025633662651586 2116 60-61 -0.10387777516266539 2116 62-63 -0.04861190309662078 2116 64-65 -0.47029870426047893 2116 66-67 -0.10754345129716114 2116 68-69 -0.03544861770540564 2116 70-71 -0.30991419657224384 2116 72-73 -0.5344625600879951 2116 74-75 -0.3232929081898277 2116 76 -0.8676574860328827 2117 1 -0.018213875565152193 2117 2 0.19360185624532278 2117 3 0.010797418215979349 2117 4 0.10174883455010075 2117 5 -0.013762526783352769 2117 6 0.030177502192238137 2117 7 0.14849384694063872 2117 8 -0.5040877441170366 2117 9 0.12072359959446999 2117 10-11 0.20481910913293433 2117 12-13 0.18280283052929036 2117 14-15 0.2020125217451607 2117 16-17 0.05735252276311087 2117 18-19 0.2601414603841903 2117 20-21 -0.013276383808971559 2117 22-23 0.18851899425264662 2117 24-25 -0.015804568610448655 2117 26-27 -0.0069225005902993075 2117 28-29 -0.4305714067783697 2117 30-31 0.13867066619634727 2117 32-33 -0.08576413742266453 2117 34-35 0.2564846134218257 2117 36-37 0.2181213288119892 2117 38-39 0.1857896854444192 2117 40-41 -0.04281348389852724 2117 42-43 0.08436398613461904 2117 44-45 0.04801260425939802 2117 46-47 0.16698955755158096 2117 48-49 0.002754236704461732 2117 50-51 0.31666498257844466 2117 52-53 0.14879430656618098 2117 54-55 0.03097803379662878 2117 56-57 0.07073165818255234 2117 58-59 0.3344932473117126 2117 60-61 0.23977542920975026 2117 62-63 0.29719453562363185 2117 64-65 0.04087213565079395 2117 66-67 0.4236459733746045 2117 68-69 0.23216693753929007 2117 70-71 0.8324340778427413 2117 72-73 0.6782829885621098 2117 74-75 0.9295447649182265 2117 76 -0.08124234644734685 2118 1 -0.07586225324610041 2118 2 -0.24454062017076694 2118 3 -0.32932906767597814 2118 4 -0.0666487287986044 2118 5 0.15908742456822722 2118 6 -0.07226972484782124 2118 7 0.019586109726716927 2118 8 -0.1273374585719651 2118 9 -0.040239216091478625 2118 10-11 -0.022802617674464898 2118 12-13 0.038100220435794085 2118 14-15 0.007276069272549535 2118 16-17 -0.10137352317791937 2118 18-19 -0.31141401996607954 2118 20-21 -0.025665867289660582 2118 22-23 0.03268541415197035 2118 24-25 -0.06363865634682497 2118 26-27 -0.25248597620264235 2118 28-29 0.00535545137975646 2118 30-31 0.13467832303462046 2118 32-33 -0.15219038218767622 2118 34-35 0.023194288111959338 2118 36-37 -0.1285462544191205 2118 38-39 -0.10190226429965321 2118 40-41 0.099229326711054 2118 42-43 -0.06207304588873086 2118 44-45 -0.06764372638064486 2118 46-47 -0.14530685938652965 2118 48-49 -0.03800467864729029 2118 50-51 0.09051857198578261 2118 52-53 -0.10116867833165344 2118 54-55 0.15473538393834474 2118 56-57 -0.03623141132123919 2118 58-59 -0.16422179985610796 2118 60-61 0.13425066081853032 2118 62-63 0.24982049272841778 2118 64-65 0.038455578266813006 2118 66-67 -0.022042296010738482 2118 68-69 -0.11373919216163131 2118 70-71 0.07971611176972715 2118 72-73 -0.516326554988801 2118 74-75 0.5359314564026363 2118 76 0.1232396536372633 2119 1 0.07958898341532716 2119 2 0.16291657231827372 2119 3 -0.2258672428765749 2119 4 -0.22329940296520334 2119 5 -0.0848947079489406 2119 6 0.02420227449042045 2119 7 -0.2079554569868236 2119 8 -0.031878777126024715 2119 9 0.1556413364769753 2119 10-11 0.07821897220063079 2119 12-13 -0.1053463011954392 2119 14-15 -0.5395536933215368 2119 16-17 -0.1423715379020507 2119 18-19 -0.03690827093803506 2119 20-21 0.013119392036998079 2119 22-23 0.11375084553587556 2119 24-25 0.03155118565830861 2119 26-27 0.157297711447292 2119 28-29 0.21865886770865472 2119 30-31 -0.08619395755103199 2119 32-33 -0.19154434033143986 2119 34-35 0.0638924434597925 2119 36-37 -0.015685389169718178 2119 38-39 -0.11282128158809712 2119 40-41 -0.14235393079576397 2119 42-43 -0.12015270508304354 2119 44-45 -0.09643022650472233 2119 46-47 -0.1620266046094585 2119 48-49 -0.06213818844958752 2119 50-51 0.032997271630087255 2119 52-53 -0.19649294005719753 2119 54-55 -0.1511211798800005 2119 56-57 -0.2130657235058706 2119 58-59 -0.1598686689106188 2119 60-61 -0.2029360378331333 2119 62-63 0.17414219013928545 2119 64-65 -0.2782683029475166 2119 66-67 0.0545252447287794 2119 68-69 -0.19545604219057822 2119 70-71 -0.2626612560853516 2119 72-73 -0.5369341321250474 2119 74-75 -0.566093111386266 2119 76 -1.8205315433186513 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 1.0 22 7.0 23 21.0 24 40.0 25 76.0 26 122.0 27 240.0 28 415.0 29 659.0 30 1074.0 31 1613.0 32 2603.0 33 4170.0 34 6720.0 35 11679.0 36 16990.0 37 73601.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.431647963476706 15.46158139844876 12.243299759232878 35.86347087884165 2 19.24470770538102 17.975889963593346 36.638257812434915 26.14114451859072 3 18.57155948780752 17.390217690135213 32.760157623320254 31.278065198737014 4 15.589046345588297 23.469379254038472 30.48994859746903 30.451625802904204 5 16.242200061649715 28.638790999141904 38.30280006331592 16.816208875892464 6 29.1053293677572 36.90235185324036 15.959777727791524 18.032541051210917 7 27.670723884265158 23.58768005465164 20.96006931427191 27.78152674681129 8 23.777627819016438 36.63325918705689 15.386602017778445 24.202510976148226 9 22.055601376288188 15.577382886372915 16.088908883390403 46.2781068539485 10-11 26.97716461306474 25.79957178442595 22.631692951105112 24.591570651404197 12-13 25.488823906758974 17.760532520223606 25.241808502661765 31.50883507035565 14-15 22.639190889172145 17.16819541292811 19.66334258079028 40.52927111710946 16-17 29.079503136637424 24.016728732931778 17.919655428090607 28.98411270234019 18-19 31.37220597668974 22.226804295485408 18.332042021777344 28.068947706047503 20-21 25.41217831762932 20.171952713003925 18.347454450026245 36.068414519340514 22-23 25.01270483950247 24.05463497538177 18.04003898927795 32.89262119583781 24-25 30.22543800454875 25.287212683178794 18.064199011938385 26.423150300334076 26-27 29.930102555130674 27.03756466971583 18.649871285396515 24.382461489756984 28-29 30.132546882940524 21.47201186340423 18.95811985037448 29.437321403280762 30-31 30.16712070847184 24.830671565319538 19.557121791507335 25.44508593470129 32-33 25.617538510243016 24.66696658418935 20.017828430514943 29.697666475052692 34-35 27.432039522464656 21.08711770929661 20.563511700948904 30.917331067289826 36-37 26.67016570443128 22.233052577207935 25.665858555563887 25.430923162796898 38-39 22.169320103638164 22.663767463947416 27.887330983979407 27.279581448435014 40-41 22.893287679221547 18.108770088225736 27.761532245299207 31.236409987253506 42-43 27.87025234727117 17.979222380512024 26.62684428448843 27.523680987728376 44-45 22.01977789441237 23.651412528221407 29.900110802862546 24.42869877450368 46-47 25.87580082144077 20.137795439587446 26.790965817733458 27.195437921238323 48-49 25.66044337807103 18.638207826181134 23.085734756275357 32.615614039472476 50-51 23.05782576458141 19.380920246932092 27.25625453000425 30.30499945848225 52-53 19.280114635142002 19.180142127581583 35.16824539918189 26.371497838094527 54-55 19.846208959202887 20.10863679154899 30.33874017978389 29.70641406946423 56-57 23.85802237717961 21.215832312780652 24.51409195804487 30.412053351994867 58-59 20.935909291611473 21.42285871385369 30.465788574808595 27.175443419726243 60-61 24.479101580398723 25.578382611448518 24.756108736764055 25.1864070713887 62-63 19.921188339873204 34.228503828113936 20.390642573292343 25.45966525872052 64-65 19.203885598127183 36.21712362433664 19.27386635341948 25.305124424116705 66-67 19.518382444827672 35.471495338781835 19.11765931035632 25.892462906034176 68-69 19.125990352653023 35.97385718927295 19.382586455391433 25.517566002682596 70-71 20.02990844184516 34.930810693725896 19.08266893271017 25.956611931718776 72-73 20.274424533253356 31.98287137703798 19.567535594378214 28.17516849533045 74-75 20.11363541692701 30.762373680571176 20.338157006823124 28.785833895678685 76 20.48686611181925 30.763206784800843 20.317745953196205 28.4321811501837 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 2.0 15 3.0 16 2.5 17 3.0 18 4.0 19 6.5 20 12.5 21 17.0 22 20.5 23 45.0 24 78.5 25 91.0 26 132.0 27 182.0 28 275.0 29 359.0 30 398.0 31 539.0 32 813.0 33 985.0 34 1082.5 35 1335.0 36 1601.0 37 1712.0 38 2050.5 39 2723.5 40 3453.5 41 6054.5 42 8260.0 43 9195.5 44 9704.5 45 8149.0 46 7020.0 47 6359.5 48 5198.0 49 4243.0 50 3789.0 51 3527.5 52 2905.5 53 2394.0 54 2243.0 55 2246.5 56 2059.5 57 1894.5 58 1920.0 59 2055.5 60 2376.0 61 2771.0 62 2981.0 63 3143.5 64 3467.0 65 3669.0 66 3704.5 67 3699.0 68 3654.5 69 3500.0 70 3186.5 71 2983.0 72 2820.5 73 2404.5 74 1880.0 75 1609.0 76 1294.0 77 770.0 78 411.5 79 262.0 80 170.0 81 47.5 82 11.0 83 5.0 84 3.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 120033.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.10009747319487 #Duplication Level Percentage of deduplicated Percentage of total 1 61.14159046827376 27.57491689785309 2 21.63295465041101 19.512967267334815 3 8.140759213078415 11.01447102046937 4 3.2973122748683843 5.9483641998450425 5 1.7142329361780733 3.8656036256696074 6 1.126812598134294 3.049161480592837 7 0.7518241433453403 2.373513950330326 8 0.5523228964625473 1.9927853173710561 9 0.371293987254087 1.5070855514733448 >10 1.2394938579477233 7.867003240775454 >50 0.011083402604599613 0.35740171452850467 >100 0.011083402604599613 0.871427024235002 >500 0.0018472337674332688 0.4748694109119992 >1k 0.005541701302299806 5.989186307098881 >5k 0.0018472337674332688 7.601242991510667 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 9124 7.601242991510667 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 2618 2.1810668732765155 No Hit TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 2398 1.9977839427490773 No Hit CACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCTG 2173 1.810335491073288 TruSeq Adapter, Index 2 (95% over 24bp) TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 570 0.4748694109119992 No Hit ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT 278 0.23160297584830838 TruSeq Adapter, Index 2 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT 249 0.20744295318787334 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT 181 0.1507918655703015 No Hit CTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 128 0.10663734139778228 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00416552114835087 0.0024993126890105223 2 0.0 0.0 0.0 0.00416552114835087 0.0024993126890105223 3 0.0 0.0 0.0 0.005831729607691218 0.0024993126890105223 4 0.0 0.0 0.0 0.03082485649779644 0.0024993126890105223 5 0.0 0.0 0.0 0.038322794564828004 0.0024993126890105223 6 0.0 0.0 0.0 0.038322794564828004 0.0024993126890105223 7 0.0 0.0 0.0 0.038322794564828004 0.0024993126890105223 8 0.0 0.0 0.0 0.03998900302416836 0.0024993126890105223 9 0.0 0.0 0.0 0.049153149550540266 0.0024993126890105223 10 0.0 0.0 0.0 0.05165246223955079 0.0024993126890105223 11 0.0 0.0 0.0 0.05165246223955079 0.0024993126890105223 12 0.0 0.0 0.0 0.05165246223955079 0.0024993126890105223 13 0.0 0.0 0.0 0.05665108761757184 0.0024993126890105223 14 0.0 0.0 0.0 0.05831729607691218 0.0024993126890105223 15 0.0 0.0 0.0 0.0608166087659227 0.0024993126890105223 16 0.0 0.0 0.0 0.0608166087659227 0.0024993126890105223 17 0.0 0.0 0.0 0.06498212991427357 0.0024993126890105223 18 0.0 0.0 0.0 0.0708138595219648 0.0024993126890105223 19 0.0 0.0 0.0 0.07914490181866653 0.0024993126890105223 20 0.0 0.0 0.0 0.0833104229670174 0.0024993126890105223 21 0.0 0.0 0.0 0.08497663142635775 0.0024993126890105223 22 0.0 0.0 0.0 0.09080836103404898 0.0024993126890105223 23 0.0 0.0 0.0 0.10413802870877176 0.0024993126890105223 24 0.0 0.0 0.0 0.10996975831646297 0.0024993126890105223 25 0.0 0.0 0.0 0.11996700907250506 0.0024993126890105223 26 0.0 0.0 0.0 0.13496288520656818 0.0024993126890105223 27 0.0 0.0 0.0 0.22993676738896804 0.0024993126890105223 28 0.0 0.0 0.0 0.3665658610548766 0.0024993126890105223 29 0.0 0.0 0.0 0.48903218281639216 0.0024993126890105223 30 0.0 0.0 0.0 0.6389909441570235 0.0024993126890105223 31 0.0 0.0 0.0 0.8122766239284197 0.0024993126890105223 32 0.0 0.0 0.0 1.0013912840635493 0.0024993126890105223 33 0.0 0.0 0.0 1.2313280514525171 0.0024993126890105223 34 0.0 0.0 0.0 1.5720676813876184 0.0024993126890105223 35 0.0 0.0 0.0 2.0277756950172035 0.0024993126890105223 36 0.0 0.0 0.0 2.6792632026192797 0.0024993126890105223 37 0.0 0.0 0.0 3.5165329534378045 0.0024993126890105223 38 0.0 0.0 0.0 4.3154799096915015 0.0024993126890105223 39 0.0 0.0 0.0 5.131922054768272 0.0024993126890105223 40 0.0 0.0 0.0 5.977522847883499 0.0024993126890105223 41 0.0 0.0 0.0 6.838119517132789 0.0024993126890105223 42 0.0 0.0 0.0 7.70621412444911 0.0024993126890105223 43 0.0 0.0 0.0 8.633459132072014 0.0024993126890105223 44 0.0 0.0 0.0 9.518215823981738 0.0024993126890105223 45 0.0 0.0 0.0 10.402972515891463 0.0024993126890105223 46 0.0 0.0 0.0 11.30189197970558 0.0024993126890105223 47 0.0 0.0 0.0 12.169986587021903 0.0024993126890105223 48 0.0 0.0 0.0 12.988928044787684 0.0024993126890105223 49 0.0 0.0 0.0 13.793706730649072 0.0024993126890105223 50 0.0 0.0 0.0 14.523506035840144 0.0024993126890105223 51 0.0 0.0 0.0 15.242474986045504 0.0024993126890105223 52 0.0 0.0 0.0 15.983104646222289 0.0024993126890105223 53 0.0 0.0 0.0 16.731232244466106 0.0024993126890105223 54 0.0 0.0 0.0 17.38688527321653 0.0024993126890105223 55 0.0 0.0 0.0 18.111685953029582 0.0024993126890105223 56 0.0 0.0 0.0 18.786500379062424 0.0024993126890105223 57 0.0 0.0 0.0 19.403830613248022 0.0024993126890105223 58 0.0 0.0 0.0 20.04282155740505 0.0024993126890105223 59 0.0 0.0 0.0 20.670982146576357 0.0024993126890105223 60 0.0 0.0 0.0 21.29081169345097 0.0024993126890105223 61 0.0 0.0 0.0 21.904809510717886 0.0024993126890105223 62 0.0 0.0 0.0 22.558796331008974 0.0024993126890105223 63 0.0 0.0 0.0 23.21611556821874 0.0024993126890105223 64 0.0 0.0 0.0 23.83677821932302 0.0024993126890105223 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAACT 30 8.334635E-6 58.333332 34 TAACTCG 30 8.334635E-6 58.333332 36 AGTCATG 25 2.3823878E-4 56.000004 37 GTTGAAA 20 0.0069369497 52.5 70 ATAATAC 20 0.0069369497 52.5 4 TGTCTGA 20 0.0069369497 52.5 48 ACGTAAC 35 2.0762105E-5 50.0 33 CACGTAA 35 2.0762105E-5 50.0 32 GTAACTC 35 2.0762105E-5 50.0 35 TCTTTAT 75 1.4551915E-11 46.666668 1 CTTTATA 80 2.910383E-11 43.75 2 TTTATAC 100 7.2759576E-12 38.500004 3 ACTCGTA 55 6.6492194E-6 38.181816 38 AAACAAT 50 1.7013882E-4 35.000004 68 CTTGAAA 1985 0.0 34.735516 57 TCGTATG 2060 0.0 34.660194 40 CTCGTAT 2040 0.0 34.656864 39 GTATGCC 2055 0.0 34.574207 42 TATGCCG 2045 0.0 34.57213 43 CTGCTTG 2005 0.0 34.56359 54 >>END_MODULE