FastQCFastQC Report
Wed 25 May 2016
SRR1780279_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780279_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485378
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGTC353.2149517E-431.42871910
TAACCGG300.00574732329.3274222
CTATAAC300.00577344229.3002264
TTACGCG300.00577344229.3002264
ACTACAT300.0057763529.2972071
TTTTCAA655.9507147E-727.06030531
CATTAAG450.001386089124.47738316
GAGAGAA551.5927623E-423.99763723
TAGTATC551.6039397E-423.9729148
GAGTATT803.56304E-622.0319221
TAGCAGA500.002595193221.975174
CTATACA703.240921E-521.9729081
CGCTTAC602.8933593E-421.9729061
CATTTGA856.030288E-620.73378216
TGTTTCT654.8953865E-420.32665635
TAAGCCG550.004455665620.01867740
GGAGATA901.0079368E-519.53751242
TAGATCT1705.456968E-1219.42989339
TATTGCC808.995454E-519.24413527
TACAAGG1051.8093924E-618.833922
Produced by FastQC (version 0.11.5)