FastQCFastQC Report
Wed 25 May 2016
SRR1780279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780279_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485378
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT204484.212799096786422No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC61201.2608729691086122No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG52441.0803950735303207No Hit
CACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTTCTG41790.860978453906028RNA PCR Primer, Index 31 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC11490.2367227192002934No Hit
ATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTT5220.10754504736514635No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACT207.854245E-443.99742521
CTTATAT250.002329581935.2669681
GTAACTC250.002352085235.197949
TCTTCTG5400.035.0349944
GTCTTCT5450.033.9062743
TCTTTAT1750.032.74791
ATGCCGT28000.032.448144
CCGTCTT5800.031.86020741
CGTCTTC5850.031.58789342
TTAATAC353.212266E-431.4332083
CTTTATA1850.030.923482
TTGACGA454.060496E-529.3376624
CATATTC300.005737531529.337663
TATCAGA300.00574331329.33161419
TTTATCA300.00574331329.33161417
AATCTAA407.0284324E-427.4983919
AATCGTC407.0284324E-427.4983928
TATGCCG33350.027.24278543
GCCGTCT7150.025.8446440
TTTATAC2750.024.8036583
Produced by FastQC (version 0.11.5)