FastQCFastQC Report
Wed 25 May 2016
SRR1780278_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780278_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107695
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGATT150.002219824669.9967525
CGTTGCT308.331119E-658.33062428
AGTCCGG200.00693629252.49756230
TTGCTGG200.00693629252.49756230
AAGACCA200.00693629252.4975624
TCTAAAG200.00693629252.4975622
TTACGCA200.00693629252.49756221
CAGTCCG352.0753405E-549.99767729
GTAGATC305.8434904E-446.68618438
ATCTAAA305.8568985E-446.66451
ACGTTGC305.8568985E-446.664527
GTATAGA305.8568985E-446.66451
CTTAGGT305.8568985E-446.66454
GACGAAC350.00125099239.99814220
CAGTCTT459.148941E-538.8870851
TAGATCT501.6960465E-435.01463739
ACGAGCA552.976405E-431.81670224
TTCTTAG450.00429227831.1096672
GGATTAA450.00429227831.1096671
AGGTGGT450.00429227831.10966732
Produced by FastQC (version 0.11.5)