FastQCFastQC Report
Wed 25 May 2016
SRR1780278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780278_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107695
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT66216.147917730628163No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG17411.6166024420818053No Hit
CACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTG17281.6045313152885463RNA PCR Primer, Index 23 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC16231.5070337527276105No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC4740.440131853846511No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT2240.20799480012999674RNA PCR Primer, Index 47 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT2190.20335205905566647No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT1200.11142578578392684No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG1120.10399740006499837No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC1080.10028320720553414No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG252.381282E-455.99999633
CTTTATA653.6379788E-1253.8461532
GGCAGTG200.0069350252.58
GTACAGC200.0069350252.513
CCGATTC200.0069350252.53
CTTTTGG200.0069350252.536
CAATTGT200.0069350252.525
CCAGATT200.0069350252.518
CCAGATG200.0069350252.54
CGCCTTT200.0069350252.527
TGCCTTG200.0069350252.513
CTACCGA200.0069350252.550
TTTATAC1050.046.6666683
TCTTTAT751.4551915E-1146.6666681
CCTCGTA753.4913683E-837.33333638
TAAAGTT400.002409739235.068
CTATCCC400.002409739235.020
CAGGCTT400.002409739235.05
GTACTGC400.002409739235.06
TCGTATG16000.035.040
Produced by FastQC (version 0.11.5)