##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780278_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 107695 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.75950601234969 34.0 34.0 34.0 34.0 34.0 2 33.732299549654115 34.0 34.0 34.0 34.0 34.0 3 33.75667394029435 34.0 34.0 34.0 34.0 34.0 4 33.764743024281536 34.0 34.0 34.0 34.0 34.0 5 33.75560610984726 34.0 34.0 34.0 34.0 34.0 6 37.51701564603742 38.0 38.0 38.0 37.0 38.0 7 37.52037699057524 38.0 38.0 38.0 37.0 38.0 8 37.43243418914527 38.0 38.0 38.0 37.0 38.0 9 37.44728167510098 38.0 38.0 38.0 37.0 38.0 10-11 37.398300756766794 38.0 38.0 38.0 37.0 38.0 12-13 37.38304935233762 38.0 38.0 38.0 37.0 38.0 14-15 37.30243743906402 38.0 38.0 38.0 36.0 38.0 16-17 37.338037977621994 38.0 38.0 38.0 36.5 38.0 18-19 37.30012071126794 38.0 38.0 38.0 36.0 38.0 20-21 37.32035841961094 38.0 38.0 38.0 36.5 38.0 22-23 37.30619341659316 38.0 38.0 38.0 36.0 38.0 24-25 37.31034866985468 38.0 38.0 38.0 36.0 38.0 26-27 37.2267143321417 38.0 38.0 38.0 36.0 38.0 28-29 37.18479502298157 38.0 38.0 38.0 36.0 38.0 30-31 37.15562932355263 38.0 38.0 38.0 36.0 38.0 32-33 37.078011978271974 38.0 38.0 38.0 36.0 38.0 34-35 37.155698964668744 38.0 38.0 38.0 36.0 38.0 36-37 37.15555968243651 38.0 38.0 38.0 36.0 38.0 38-39 36.53485769998607 38.0 37.0 38.0 34.0 38.0 40-41 36.82679325873996 38.0 37.5 38.0 34.5 38.0 42-43 37.0757463206277 38.0 38.0 38.0 36.0 38.0 44-45 37.152876178095546 38.0 38.0 38.0 36.0 38.0 46-47 37.09743720692697 38.0 38.0 38.0 36.0 38.0 48-49 37.10448953061888 38.0 38.0 38.0 36.0 38.0 50-51 36.97781234040578 38.0 38.0 38.0 35.5 38.0 52-53 36.99181949022703 38.0 38.0 38.0 36.0 38.0 54-55 36.95620038070477 38.0 38.0 38.0 35.5 38.0 56-57 37.014652490830585 38.0 38.0 38.0 36.0 38.0 58-59 36.90237244068898 38.0 38.0 38.0 35.5 38.0 60-61 36.90271136078741 38.0 38.0 38.0 35.0 38.0 62-63 36.87819304517387 38.0 38.0 38.0 35.0 38.0 64-65 36.785867496169736 38.0 38.0 38.0 34.5 38.0 66-67 36.499419657365706 38.0 37.5 38.0 34.0 38.0 68-69 36.39924787594596 38.0 37.0 38.0 34.0 38.0 70-71 35.7541157899624 38.0 37.0 38.0 30.5 38.0 72-73 34.70171317145643 38.0 37.0 38.0 23.5 38.0 74-75 34.37278425182228 38.0 36.5 38.0 22.0 38.0 76 33.7431357073216 38.0 34.0 38.0 21.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.17611075780524743 1101 2 -0.1878089480148688 1101 3 -0.1799575428353961 1101 4 0.035395265747872884 1101 5 0.03652672152807668 1101 6 -0.1732728903765306 1101 7 -0.06543377137430184 1101 8 -0.050243925059341166 1101 9 -0.10132560943220881 1101 10-11 0.08489300156169577 1101 12-13 -0.05228020719964377 1101 14-15 0.036386229075972665 1101 16-17 -0.06582719005071169 1101 18-19 0.11458346348764081 1101 20-21 -0.08544972515848315 1101 22-23 0.062675786576321 1101 24-25 0.014804243319439081 1101 26-27 -0.13785091666419902 1101 28-29 -0.15539489976846 1101 30-31 -0.2858377605695779 1101 32-33 0.14767506919119455 1101 34-35 0.20409481158095844 1101 36-37 0.08639451269345244 1101 38-39 0.07474169881980686 1101 40-41 0.11085997334956943 1101 42-43 0.19139573417816536 1101 44-45 -0.014166218183135015 1101 46-47 -0.38984983598942335 1101 48-49 -0.044319060639359975 1101 50-51 0.09684301361819792 1101 52-53 0.11830092910195589 1101 54-55 0.03723480350482333 1101 56-57 -0.044411130092292694 1101 58-59 -0.10013659244807371 1101 60-61 -0.020832832029704207 1101 62-63 -0.15976804031640768 1101 64-65 0.11743488272380631 1101 66-67 -0.23449785253058053 1101 68-69 -0.6122169053011319 1101 70-71 -0.09009601858108596 1101 72-73 0.013361779159673404 1101 74-75 -0.03445390225532918 1101 76 -1.4278590812374077 1102 1 0.14137516129357408 1102 2 -0.03888421683207355 1102 3 -0.0238003487749765 1102 4 0.10628620276784062 1102 5 -0.02873185502082265 1102 6 0.09668358683801159 1102 7 0.15832454915820904 1102 8 0.219866161986225 1102 9 0.19025145149969092 1102 10-11 -0.08905093084997873 1102 12-13 0.07799373033235213 1102 14-15 0.2455068127933302 1102 16-17 -0.003916027736323713 1102 18-19 0.2092007189919869 1102 20-21 0.24877966552251962 1102 22-23 0.22525899190146248 1102 24-25 0.07869313220832197 1102 26-27 0.16170105466196816 1102 28-29 0.1530280392996417 1102 30-31 0.2459850760919693 1102 32-33 0.10531075787526589 1102 34-35 0.37545937891326986 1102 36-37 0.1947534476652848 1102 38-39 0.5268666348157183 1102 40-41 0.17685459700548734 1102 42-43 0.1887267633640377 1102 44-45 0.28913639318399476 1102 46-47 0.21298554547499293 1102 48-49 0.32321806173646905 1102 50-51 0.13476544065353124 1102 52-53 0.22966165618848322 1102 54-55 0.1678863652047653 1102 56-57 0.012725326640932622 1102 58-59 0.4296381131330804 1102 60-61 0.4911103323328163 1102 62-63 0.04888531349823921 1102 64-65 0.15106263489995087 1102 66-67 0.36675022734652885 1102 68-69 0.3741758238335322 1102 70-71 0.18644007972920917 1102 72-73 -0.177568840399978 1102 74-75 0.09038608750402233 1102 76 -0.2619348620873865 1103 1 -0.042817779597015715 1103 2 0.11981817062920186 1103 3 0.021676839731192388 1103 4 0.012756040566273441 1103 5 0.009771273586189011 1103 6 0.14615810235954996 1103 7 0.1297236136135922 1103 8 0.18565195871789797 1103 9 0.1859625261610134 1103 10-11 0.08135183933408996 1103 12-13 0.08095223832093268 1103 14-15 0.14259082956023406 1103 16-17 -0.030566899493820188 1103 18-19 0.19603019472250338 1103 20-21 0.14421129179066838 1103 22-23 0.08840217882813306 1103 24-25 -0.05256863803649736 1103 26-27 -0.0111560014099652 1103 28-29 0.05223221887560925 1103 30-31 -0.05932444337830134 1103 32-33 -0.09030919231001633 1103 34-35 -0.08628049108247637 1103 36-37 0.03391267249974561 1103 38-39 0.14544387799656988 1103 40-41 -0.07452138258263119 1103 42-43 -0.02158852732303984 1103 44-45 0.07965944767643407 1103 46-47 0.022468565947626473 1103 48-49 -0.12615925434396047 1103 50-51 -0.042836163137245364 1103 52-53 0.13340383467095762 1103 54-55 -0.03658480908598705 1103 56-57 -0.009211372223049352 1103 58-59 0.10966970295143597 1103 60-61 0.10973617523422519 1103 62-63 0.11069200810974422 1103 64-65 0.07427507642841391 1103 66-67 -0.2386443416346964 1103 68-69 0.019804886466417315 1103 70-71 -0.17417592172878216 1103 72-73 -0.03902926200255763 1103 74-75 -0.024133079010773884 1103 76 0.4047075047190063 1104 1 -0.06925172280051584 1104 2 -0.012643385100957971 1104 3 0.11973498318162967 1104 4 0.1045059563817361 1104 5 0.09797421443251864 1104 6 0.07840639060738397 1104 7 0.03579612455757797 1104 8 0.02943914021975047 1104 9 0.03874061573335297 1104 10-11 0.12021703183605581 1104 12-13 0.07314552600754354 1104 14-15 0.003001650078807927 1104 16-17 0.19572673615552816 1104 18-19 0.16670030360116783 1104 20-21 0.010576764907739289 1104 22-23 0.14567011013355824 1104 24-25 0.16496980623334423 1104 26-27 0.01397027538877893 1104 28-29 -0.023275694470441977 1104 30-31 -0.04189263284962408 1104 32-33 0.013675542228085646 1104 34-35 0.07053284374747193 1104 36-37 0.15226490058370246 1104 38-39 0.024599787750744895 1104 40-41 0.014585138912480033 1104 42-43 -0.14674286563261063 1104 44-45 -0.2680451207375256 1104 46-47 -0.17032523807078093 1104 48-49 -0.208037130648826 1104 50-51 -0.3096730696362897 1104 52-53 -0.15332868300778557 1104 54-55 0.014257036238973342 1104 56-57 -0.28018454541869886 1104 58-59 0.11589935835608856 1104 60-61 -0.13112138453679734 1104 62-63 0.09283366261642101 1104 64-65 -0.1306020141541424 1104 66-67 -0.3866856481786414 1104 68-69 -0.7512187974487148 1104 70-71 -0.8198263875498597 1104 72-73 0.013137086633655315 1104 74-75 -0.356532899417104 1104 76 -0.7240274823726978 1105 1 -0.19197032196989738 1105 2 0.09947918757835339 1105 3 0.0759141278667812 1105 4 -0.014362603501524518 1105 5 -0.04785584505788165 1105 6 0.07836149219005506 1105 7 0.041588020393248826 1105 8 0.033109585836541555 1105 9 0.09104727192372053 1105 10-11 -0.16271595727607746 1105 12-13 -0.032607083712960616 1105 14-15 0.02394676176362509 1105 16-17 0.03722971067567471 1105 18-19 -0.14634268663342453 1105 20-21 -0.004941250582213286 1105 22-23 0.06975811103152552 1105 24-25 -0.015280502443275168 1105 26-27 -0.06878312005402876 1105 28-29 0.11324916802815466 1105 30-31 -0.2779685111749117 1105 32-33 -0.02420749739476946 1105 34-35 0.04422798349379775 1105 36-37 -0.06778224275449674 1105 38-39 0.035274386471144226 1105 40-41 0.061071837756344394 1105 42-43 -0.1775207307128639 1105 44-45 -0.12542529808627734 1105 46-47 -0.045328044221868424 1105 48-49 -0.1210745085812519 1105 50-51 -0.15300565466267102 1105 52-53 0.12153942789129957 1105 54-55 -0.038279724340220866 1105 56-57 -0.11260120273151841 1105 58-59 -0.14287266992991476 1105 60-61 0.08600736167489487 1105 62-63 -0.11642663595805658 1105 64-65 -0.22741983882583128 1105 66-67 -0.39627146027876137 1105 68-69 -0.1564662999742552 1105 70-71 -0.46231151494912126 1105 72-73 -0.42546993996865723 1105 74-75 -0.45972629934975373 1105 76 -0.2834280885013314 1106 1 0.07644364143789062 1106 2 -0.06562351755128759 1106 3 0.06344359245315445 1106 4 0.044903307375115276 1106 5 0.044994081982189016 1106 6 0.012181225044471944 1106 7 -0.050296590674207664 1106 8 0.006260332272731262 1106 9 0.008939291230042556 1106 10-11 0.1036607404453278 1106 12-13 0.10460247964992675 1106 14-15 -0.11289238965628812 1106 16-17 -0.027783197619307032 1106 18-19 -0.017074530835920143 1106 20-21 -0.049025884098881534 1106 22-23 -0.023204062051867425 1106 24-25 0.17821483934592663 1106 26-27 0.05867226214374455 1106 28-29 -0.018308807053223575 1106 30-31 0.01771001615700385 1106 32-33 0.11963580712875199 1106 34-35 -0.17118901055716407 1106 36-37 0.02643708601416961 1106 38-39 -0.11281743079229756 1106 40-41 -0.14601088757758873 1106 42-43 0.06848892244685345 1106 44-45 0.2816237534346584 1106 46-47 0.24358204669743344 1106 48-49 0.010505915708797886 1106 50-51 0.07112163235046154 1106 52-53 -0.08379225916672794 1106 54-55 0.040747102464131046 1106 56-57 -0.04342957853127416 1106 58-59 -0.17880262839887706 1106 60-61 0.09901106579432195 1106 62-63 -0.26478223142331814 1106 64-65 -0.014707974419046366 1106 66-67 0.020599120033281793 1106 68-69 0.12129775884267247 1106 70-71 -0.061548241854502805 1106 72-73 0.14393178862041367 1106 74-75 0.25770551117891927 1106 76 0.4713367560378998 1107 1 -0.1322332067848393 1107 2 -0.09155044461350315 1107 3 0.1025254503618811 1107 4 0.045939483434942474 1107 5 -0.07945967302974566 1107 6 -0.03874908085272466 1107 7 -0.19128411151035607 1107 8 -0.006196306011723607 1107 9 0.03506894839092212 1107 10-11 -0.2844097195267423 1107 12-13 0.048570132936404775 1107 14-15 0.15432840594671404 1107 16-17 0.02320342219418592 1107 18-19 -0.008120618145021297 1107 20-21 0.08631898232451363 1107 22-23 0.04890027637223682 1107 24-25 0.1438858759724937 1107 26-27 0.04565248469634042 1107 28-29 0.017394215877800434 1107 30-31 -0.014749325195431595 1107 32-33 -0.03438275393806123 1107 34-35 -0.283490222432647 1107 36-37 0.014115601128821709 1107 38-39 -0.054510001860464286 1107 40-41 -0.002320298759272532 1107 42-43 0.1503243056067447 1107 44-45 0.04297663895972903 1107 46-47 -0.019696774764931035 1107 48-49 -0.15350273410874848 1107 50-51 0.10662192518282154 1107 52-53 0.28165126923801154 1107 54-55 0.12116337493338847 1107 56-57 0.19478955017981292 1107 58-59 0.07231238714376786 1107 60-61 -0.2271253490365055 1107 62-63 -0.20908776820756714 1107 64-65 0.08903352217959792 1107 66-67 0.16694772570071592 1107 68-69 0.034551802181866265 1107 70-71 -0.06849737912529719 1107 72-73 -0.34114502356141685 1107 74-75 -0.20324641926212905 1107 76 -0.30915159824421323 1108 1 0.12462815845091058 1108 2 0.13805312095065148 1108 3 0.11770255568676902 1108 4 -0.0017968524787335127 1108 5 -0.12184766270837599 1108 6 0.03990444952494698 1108 7 0.11978790350254087 1108 8 0.06490385819189015 1108 9 0.21074335608503247 1108 10-11 -0.2640602004087498 1108 12-13 -0.1452605027661491 1108 14-15 0.06932957882966662 1108 16-17 0.0626826621660328 1108 18-19 0.18705883294576608 1108 20-21 0.13696406794496596 1108 22-23 0.07954770775858577 1108 24-25 0.11049389849184621 1108 26-27 0.18025253161166432 1108 28-29 0.15839820367981616 1108 30-31 0.06834943154863993 1108 32-33 -0.06026828056251077 1108 34-35 0.09165638793564312 1108 36-37 -0.002644896173542577 1108 38-39 -0.1388050992097476 1108 40-41 -0.03784692320215299 1108 42-43 0.1325903154589625 1108 44-45 0.3109569345262315 1108 46-47 0.1225270113012229 1108 48-49 0.07489276202073825 1108 50-51 0.12855483627829756 1108 52-53 -0.2754798590753893 1108 54-55 -0.11627504871193395 1108 56-57 0.08175882064661977 1108 58-59 -3.828978667925753E-4 1108 60-61 0.1987484487584723 1108 62-63 -0.1016399614986696 1108 64-65 -0.10781142269490829 1108 66-67 0.02073613761720594 1108 68-69 0.11179787302398836 1108 70-71 0.24253944939921013 1108 72-73 0.11059084319908408 1108 74-75 -0.5130869071814388 1108 76 0.40686998280200015 1109 1 -0.14264205259740947 1109 2 0.024732858005201308 1109 3 0.021416083538227326 1109 4 -7.012464355966586E-4 1109 5 -0.05398928420532201 1109 6 -0.1700597991199615 1109 7 0.13810182346563948 1109 8 -0.08988797665991655 1109 9 0.09716937384538227 1109 10-11 -0.11719132715306557 1109 12-13 0.07787985013432319 1109 14-15 -0.06578171167892322 1109 16-17 0.07734648410121991 1109 18-19 0.04580061590043982 1109 20-21 0.18294492367094506 1109 22-23 0.038081902199827766 1109 24-25 0.06605842392144012 1109 26-27 0.07310226059332336 1109 28-29 0.0588191470542867 1109 30-31 -0.216248654023957 1109 32-33 -0.02766535125792302 1109 34-35 -0.07484689888249108 1109 36-37 0.15023827762416886 1109 38-39 0.04894141214995784 1109 40-41 -0.10097113032936278 1109 42-43 -0.15259017122077267 1109 44-45 0.09851892276287799 1109 46-47 0.1214198725628961 1109 48-49 0.05663650553359645 1109 50-51 0.06832533564400478 1109 52-53 -0.09682687787368138 1109 54-55 -0.09682515827253013 1109 56-57 -0.03564978274399522 1109 58-59 0.2582723898739516 1109 60-61 0.25739220380403083 1109 62-63 -0.03124439005362234 1109 64-65 -0.07407252291402955 1109 66-67 0.018960102557812775 1109 68-69 0.1357166828498464 1109 70-71 0.4687071347872873 1109 72-73 0.0923691847975121 1109 74-75 0.03485450672699386 1109 76 0.12543762205929454 1110 1 0.13045294875846025 1110 2 -0.058754893960809795 1110 3 0.022455584577734555 1110 4 0.09804005339265842 1110 5 0.07137228137363394 1110 6 -0.19045435755799645 1110 7 0.14112993518940442 1110 8 0.09917692436150816 1110 9 0.04191763381103186 1110 10-11 -0.12461472044603283 1110 12-13 0.10635878893934603 1110 14-15 -0.03460797941824012 1110 16-17 -0.07046039468391996 1110 18-19 0.0018421507463202147 1110 20-21 0.15948270727395197 1110 22-23 -0.009187147786612115 1110 24-25 0.05804748656267833 1110 26-27 0.09458151576823326 1110 28-29 0.24899699212343052 1110 30-31 0.23930702707520624 1110 32-33 0.0011017101178296684 1110 34-35 0.08256971505586819 1110 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0.034524310827443117 2115 62-63 -0.013339468887842543 2115 64-65 0.13172059700952587 2115 66-67 0.08157357604085291 2115 68-69 -0.05150261958684865 2115 70-71 0.05990398141891973 2115 72-73 0.42050463630253176 2115 74-75 0.37090324060181246 2115 76 0.3935694901911617 2116 1 -0.036163667858154724 2116 2 0.08070957050365024 2116 3 -0.08855542643328107 2116 4 0.030897911250512777 2116 5 0.09922513422648649 2116 6 0.22738848528484823 2116 7 0.09117998523944948 2116 8 0.11126401144859699 2116 9 -0.010584868691466909 2116 10-11 0.16525014441883457 2116 12-13 0.17906899914956398 2116 14-15 0.2991507793405219 2116 16-17 -0.2964488831724026 2116 18-19 0.10710991851409091 2116 20-21 0.0015873118785521 2116 22-23 0.04270224160277536 2116 24-25 0.052767206282403833 2116 26-27 0.14455649074320576 2116 28-29 0.18349398912042858 2116 30-31 0.1022574775256544 2116 32-33 0.033984593000710106 2116 34-35 0.2230101554963042 2116 36-37 -0.017970566671628774 2116 38-39 -0.06811544403733194 2116 40-41 -0.027565952576360075 2116 42-43 0.11196451724695322 2116 44-45 -0.07077997479689202 2116 46-47 -0.18277311641269733 2116 48-49 0.23134231502201175 2116 50-51 0.08870809298328197 2116 52-53 0.1764358497368761 2116 54-55 0.17433797810799234 2116 56-57 0.08303595985479006 2116 58-59 -0.016538708850191597 2116 60-61 -0.006679392876264956 2116 62-63 0.12594624539787702 2116 64-65 0.10566239595132032 2116 66-67 0.0635841580514338 2116 68-69 -0.06949203757626776 2116 70-71 0.21559181210674438 2116 72-73 -0.3244027711048787 2116 74-75 0.007411177109752032 2116 76 -0.009605112983443576 2117 1 1.0708565397266057E-4 2117 2 0.039968829762912605 2117 3 -0.21907905350862933 2117 4 0.10356713219560021 2117 5 0.07891862081652334 2117 6 0.15637954531038645 2117 7 0.3259181717017796 2117 8 -0.30750993491155754 2117 9 0.042927264131009224 2117 10-11 0.08459880342266501 2117 12-13 0.03615712175492547 2117 14-15 0.20898475124346305 2117 16-17 0.12072863917752841 2117 18-19 -0.03842009425729742 2117 20-21 -0.10173325006269351 2117 22-23 0.0831875545018832 2117 24-25 0.028884703089552488 2117 26-27 0.11856670785687129 2117 28-29 -0.32225313731634486 2117 30-31 0.07505441239155175 2117 32-33 0.16846735162140192 2117 34-35 0.16541117720767318 2117 36-37 -0.030805815713776497 2117 38-39 0.2901221038553885 2117 40-41 -0.04493504580752017 2117 42-43 -0.11185412898548464 2117 44-45 -0.08578636049293209 2117 46-47 -0.17479099636161521 2117 48-49 -0.016294977442861125 2117 50-51 0.026064414822357662 2117 52-53 0.10229791870239069 2117 54-55 0.32108127312715595 2117 56-57 -0.021881026096671974 2117 58-59 0.3551726576887617 2117 60-61 0.16618139895005157 2117 62-63 0.06464356340553934 2117 64-65 -0.1650911161814932 2117 66-67 0.11115759355590171 2117 68-69 -0.2027615139491843 2117 70-71 0.1892827444183709 2117 72-73 0.2830046363025289 2117 74-75 0.3478326330484478 2117 76 -0.2188005152822896 2118 1 -0.26042931281345716 2118 2 -0.2512189097006896 2118 3 -0.2957074059990532 2118 4 -0.6473907222105311 2118 5 -0.21226911864707887 2118 6 -0.22676221714171874 2118 7 0.11519020235311928 2118 8 -0.1121076360609834 2118 9 0.04675868175552722 2118 10-11 0.10375589154527631 2118 12-13 0.028494286505875266 2118 14-15 -0.06879302653432262 2118 16-17 0.009617528066414138 2118 18-19 -0.19550821686267028 2118 20-21 -0.2952198401010122 2118 22-23 -0.18118026159007883 2118 24-25 0.044210373587638685 2118 26-27 -0.011701491376847173 2118 28-29 0.02449015770280738 2118 30-31 0.0961272093264256 2118 32-33 0.009463520203780718 2118 34-35 0.02173301628813107 2118 36-37 0.06881104252376957 2118 38-39 -0.06236831760055139 2118 40-41 0.008704800935774415 2118 42-43 0.07205391699152841 2118 44-45 0.03490329467948072 2118 46-47 0.3596917622590752 2118 48-49 0.09098471604372804 2118 50-51 -0.02182830548414927 2118 52-53 -0.44176338397960535 2118 54-55 0.09885905090492741 2118 56-57 -0.290080259813152 2118 58-59 -0.14482734231123828 2118 60-61 -0.04837798802313387 2118 62-63 -0.04072042126879438 2118 64-65 -0.18999533074088504 2118 66-67 -0.03443627617589584 2118 68-69 -0.08207185877677148 2118 70-71 -0.19194330922147884 2118 72-73 0.09526517270099788 2118 74-75 0.8669897211710591 2118 76 0.3367550402732604 2119 1 0.14457154496660962 2119 2 0.1465194101940881 2119 3 -0.07244155136417874 2119 4 -0.16854929715191957 2119 5 -0.07349460043354838 2119 6 -0.09933898845755351 2119 7 -0.28878131934871476 2119 8 0.1338179086293536 2119 9 0.14942065922450354 2119 10-11 0.04622029367256886 2119 12-13 -0.09646997265807045 2119 14-15 -0.5078834937385253 2119 16-17 0.04155553916037036 2119 18-19 0.2070408195643978 2119 20-21 -0.014554202770426627 2119 22-23 0.1590510531008391 2119 24-25 0.07972961645376841 2119 26-27 0.08005953109699249 2119 28-29 0.09311256291810821 2119 30-31 -0.0804539652604177 2119 32-33 -0.694995506501769 2119 34-35 -0.35475380249152977 2119 36-37 -0.0580617772852321 2119 38-39 -0.5140109664253885 2119 40-41 0.01552415245404859 2119 42-43 0.07782963609714955 2119 44-45 -0.07775896871618215 2119 46-47 0.07952649663318567 2119 48-49 0.03968946814529062 2119 50-51 -0.2596335322240151 2119 52-53 -0.09686431610060708 2119 54-55 0.03880729817432638 2119 56-57 -0.05637808105178266 2119 58-59 -0.011757061531227464 2119 60-61 0.029486997394606362 2119 62-63 0.09049848420384876 2119 64-65 -0.10239454158323014 2119 66-67 -0.04054795914038323 2119 68-69 0.04428629299310671 2119 70-71 -0.019786850137585077 2119 72-73 -0.2617714831004534 2119 74-75 -0.31443791078411465 2119 76 -0.7132535375273861 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 3.0 20 1.0 21 2.0 22 9.0 23 12.0 24 28.0 25 49.0 26 88.0 27 169.0 28 275.0 29 513.0 30 839.0 31 1314.0 32 2002.0 33 3405.0 34 5676.0 35 9740.0 36 14635.0 37 68935.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.513440735410185 15.58011049723757 11.053437949765542 33.85301081758671 2 21.998235758391754 18.5254654347927 33.24202609220484 26.234272714610707 3 19.172663540554343 17.992478759459583 32.13891081294396 30.69594688704211 4 14.954269000417847 21.936951576210596 32.56882863642694 30.539950786944615 5 14.713775012767538 29.738613677515207 38.59324945447792 16.954361855239334 6 28.476716653512234 37.52634755559682 17.060216351734063 16.936719439156878 7 27.57602488509216 25.774641348252008 21.350109104415246 25.299224662240587 8 24.425460792051627 36.22266586192488 16.077812340405774 23.274061005617718 9 22.588792423046566 16.783508983703978 16.911648637355494 43.71604995589396 10-11 25.52811179720507 26.25005803426343 24.006685547147036 24.215144621384464 12-13 24.91759134593064 19.689864896234738 25.90974511351502 29.482798644319608 14-15 21.903059566367983 19.096986861042758 20.97497562560936 38.0249779469799 16-17 26.735224476530945 25.685036445517433 20.203352059055664 27.376387018895954 18-19 28.573285667858308 24.103254561493106 20.992618041691816 26.330841728956777 20-21 23.93333023817262 22.46854542922141 20.868192580899763 32.72993175170621 22-23 22.709503690979155 26.678118761316682 20.646269557546777 29.966107990157386 24-25 26.695296903291705 26.68461859882074 21.704814522494082 24.91526997539347 26-27 28.613677515204976 27.30767445099587 21.278146617763127 22.800501416036028 28-29 23.344166395840105 27.45345652072984 22.770787873160316 26.431589210269745 30-31 23.053995078694463 28.35368401504248 24.829379265518362 23.762941640744696 32-33 29.289196341520036 22.84692882677933 24.474209573332097 23.38966525836854 34-35 25.34240215423186 24.415711035795535 27.348066298342545 22.893820511630068 36-37 27.169785041088257 26.40698268257579 23.725799712150053 22.697432564185895 38-39 22.459259947072752 24.354891127721807 27.316495659037095 25.869353266168343 40-41 21.70760016713868 20.41506105204513 28.828172152839038 29.049166627977158 42-43 25.670179674079574 20.19778076976647 27.923301917452065 26.20873763870189 44-45 21.061330609591902 24.511351501926736 30.41691814847486 24.0103997400065 46-47 23.890152746181347 21.234504851664422 28.567714378569107 26.30762802358512 48-49 23.553089744184966 20.3542411439714 25.786248200937834 30.3064209109058 50-51 21.555318259900645 21.04693811226148 28.776173452806535 28.62157017503134 52-53 18.00269278982311 21.357537490134177 35.11769348623427 25.522076233808438 54-55 18.608570500023212 22.142625005803428 30.895120479130878 28.35368401504248 56-57 21.776312735038765 22.82835786248201 26.47058823529412 28.924741167185108 58-59 18.870885370722874 22.804679883002926 31.556246808115514 26.76818793815869 60-61 22.114768559357444 26.522586935326615 26.593156599656435 24.769487905659503 62-63 18.609963322345514 33.525233297738986 22.501508890849156 25.363294489066345 64-65 18.224151539068668 34.996053670086816 21.25261154185431 25.527183248990205 66-67 18.196295092622687 34.62927712521473 20.655555039695436 26.518872742467153 68-69 17.986907470170387 34.944054970054324 20.839407586238913 26.22962997353638 70-71 18.41682529365337 33.82701146757046 21.469427550025536 26.28673568875064 72-73 18.862992710896513 32.21365894424068 21.458284971447142 27.465063373415667 74-75 19.590045963136635 31.40117925623288 22.068805422721574 26.93996935790891 76 19.802219230233533 31.888202794930127 21.754956126096847 26.554621848739497 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 1.0 14 3.5 15 7.0 16 6.0 17 5.0 18 10.0 19 13.0 20 11.5 21 12.0 22 28.5 23 55.0 24 87.5 25 110.0 26 145.5 27 211.5 28 292.5 29 343.0 30 434.5 31 592.5 32 825.5 33 992.0 34 1121.0 35 1479.5 36 1829.5 37 1950.0 38 2306.5 39 4602.0 40 7489.5 41 7955.0 42 7472.0 43 6985.5 44 6334.5 45 5859.5 46 5549.0 47 5549.0 48 5289.0 49 4944.0 50 4859.0 51 4487.5 52 3701.0 53 3092.0 54 2898.0 55 2785.0 56 2522.5 57 2291.5 58 2210.0 59 2164.5 60 2107.5 61 2104.5 62 2113.0 63 2049.5 64 2097.5 65 2222.5 66 2207.0 67 2178.0 68 2095.0 69 1853.5 70 1583.0 71 1471.0 72 1355.0 73 1040.0 74 713.5 75 586.0 76 431.5 77 198.0 78 80.0 79 41.0 80 28.0 81 8.5 82 1.5 83 1.0 84 1.5 85 1.0 86 0.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 107695.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.64292678397326 #Duplication Level Percentage of deduplicated Percentage of total 1 65.61104111785592 31.91513069316124 2 19.5777497804757 19.046380983332558 3 6.455923338296492 9.421050188031014 4 2.7965486962165462 5.441292539115094 5 1.6550223342114305 4.025256511444357 6 1.0365364792120033 3.0252100840336134 7 0.7368380865116635 2.5089372765680857 8 0.48104455388844347 1.8719532011699709 9 0.3455121597373344 1.5126050420168067 >10 1.2713320352766007 8.774780630484239 >50 0.01336234871912343 0.4141325038302614 >100 0.011453441759248655 1.1671851060866334 >500 0.0 0.0 >1k 0.005726720879624328 4.728167510097961 >5k 0.0019089069598747757 6.147917730628163 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT 6621 6.147917730628163 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG 1741 1.6166024420818053 No Hit CACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTG 1728 1.6045313152885463 RNA PCR Primer, Index 23 (95% over 24bp) CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 1623 1.5070337527276105 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 474 0.440131853846511 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT 224 0.20799480012999674 RNA PCR Primer, Index 47 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT 219 0.20335205905566647 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT 120 0.11142578578392684 No Hit CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG 112 0.10399740006499837 No Hit TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC 108 0.10028320720553414 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0037141928594642275 0.0 2 0.0 0.0 0.0 0.004642741074330285 0.0 3 0.0 0.0 0.0 0.00928548214866057 0.0 4 0.0 0.0 0.0 0.011142578578392683 0.0 5 0.0 0.0 0.0 0.01207112679325874 0.0 6 0.0 0.0 0.0 0.01207112679325874 0.0 7 0.0 0.0 0.0 0.012999675008124796 0.0 8 0.0 0.0 0.0 0.01485677143785691 0.0 9 0.0 0.0 0.0 0.020428060727053252 0.0 10 0.0 0.0 0.0 0.022285157156785366 0.0 11 0.0 0.0 0.0 0.022285157156785366 0.0 12 0.0 0.0 0.0 0.023213705371651423 0.0 13 0.0 0.0 0.0 0.02414225358651748 0.0 14 0.0 0.0 0.0 0.025070801801383536 0.0 15 0.0 0.0 0.0 0.027856446445981706 0.0 16 0.0 0.0 0.0 0.028784994660847763 0.0 17 0.0 0.0 0.0 0.028784994660847763 0.0 18 0.0 0.0 0.0 0.035284832164910164 0.0 19 0.0 0.0 0.0 0.040856121454106505 0.0 20 0.0 0.0 0.0 0.04271321788383862 0.0 21 0.0 0.0 0.0 0.04549886252843679 0.0 22 0.0 0.0 0.0 0.051998700032499186 0.0 23 0.0 0.0 0.0 0.05942708575142764 0.0 24 0.0 0.0 0.0 0.06592692325549004 0.0 25 0.0 0.0 0.0 0.07428385718928456 0.0 26 0.0 0.0 0.0 0.0947119179163378 0.0 27 0.0 0.0 0.0 0.21078044477459493 0.0 28 0.0 0.0 0.0 0.3017781698314685 0.0 29 0.0 0.0 0.0 0.4197037931194577 0.0 30 0.0 0.0 0.0 0.5571289289196342 0.0 31 0.0 0.0 0.0 0.7075537397279353 0.0 32 0.0 0.0 0.0 0.8746924184038256 0.0 33 0.0 0.0 0.0 1.0724731881702958 0.0 34 0.0 0.0 0.0 1.321324109754399 0.0 35 0.0 0.0 0.0 1.679743720692697 0.0 36 0.0 0.0 0.0 2.18765959422443 0.0 37 0.0 0.0 0.0 2.8961418821672313 0.0 38 0.0 0.0 0.0 3.690979154092576 0.0 39 0.0 0.0 0.0 4.559171734992339 0.0 40 0.0 0.0 0.0 5.52207623380844 0.0 41 0.0 0.0 0.0 6.634476995217977 0.0 42 0.0 0.0 0.0 7.995728678211616 0.0 43 0.0 0.0 0.0 9.232554900413204 0.0 44 0.0 0.0 0.0 10.324527601095687 0.0 45 0.0 0.0 0.0 11.410000464274107 0.0 46 0.0 0.0 0.0 12.49733042388226 0.0 47 0.0 0.0 0.0 13.519662008449789 0.0 48 0.0 0.0 0.0 14.404568457217142 0.0 49 0.0 0.0 0.0 15.282046520265565 0.0 50 0.0 0.0 0.0 16.108454431496355 0.0 51 0.0 0.0 0.0 17.018431682065092 0.0 52 0.0 0.0 0.0 18.128975347044896 0.0 53 0.0 0.0 0.0 19.33608802637077 0.0 54 0.0 0.0 0.0 20.41506105204513 0.0 55 0.0 0.0 0.0 21.36589442406797 0.0 56 0.0 0.0 0.0 22.212730396025815 0.0 57 0.0 0.0 0.0 22.981568317934908 0.0 58 0.0 0.0 0.0 23.73462092019128 0.0 59 0.0 0.0 0.0 24.41988950276243 0.0 60 0.0 0.0 0.0 25.09122986211059 0.0 61 0.0 0.0 0.0 25.75699893216955 0.0 62 0.0 0.0 0.0 26.42369655044338 0.0 63 0.0 0.0 0.0 27.26496123311203 0.0 64 0.0 0.0 0.0 28.095083337202283 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTGGAG 25 2.381282E-4 55.999996 33 CTTTATA 65 3.6379788E-12 53.846153 2 GGCAGTG 20 0.00693502 52.5 8 GTACAGC 20 0.00693502 52.5 13 CCGATTC 20 0.00693502 52.5 3 CTTTTGG 20 0.00693502 52.5 36 CAATTGT 20 0.00693502 52.5 25 CCAGATT 20 0.00693502 52.5 18 CCAGATG 20 0.00693502 52.5 4 CGCCTTT 20 0.00693502 52.5 27 TGCCTTG 20 0.00693502 52.5 13 CTACCGA 20 0.00693502 52.5 50 TTTATAC 105 0.0 46.666668 3 TCTTTAT 75 1.4551915E-11 46.666668 1 CCTCGTA 75 3.4913683E-8 37.333336 38 TAAAGTT 40 0.0024097392 35.0 68 CTATCCC 40 0.0024097392 35.0 20 CAGGCTT 40 0.0024097392 35.0 5 GTACTGC 40 0.0024097392 35.0 6 TCGTATG 1600 0.0 35.0 40 >>END_MODULE