FastQCFastQC Report
Wed 25 May 2016
SRR1780277_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780277_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645549
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCTA300.00573775729.33886523
ACTTATT450.001395971324.44905723
GACTATC803.5786907E-622.02294738
CGTCATA500.002576319322.0041523
GGTATAG904.1356907E-721.9666541
TATCCAC500.002601962521.9666543
ATAACGC550.004520897319.9696875
CTTTATA550.004520897319.9696872
TTACGAG708.0609944E-418.87681236
TAATCTC951.6162092E-518.4982365
GAGCGAA600.0074335718.32257529
AGCGAAA600.0074335718.32257530
GAAAGTC600.00744687818.31689631
TAACGCT600.007473552618.3055446
GTTGTTC851.4393774E-418.10140231
TATAAGA2200.017.9727172
GATATGT1002.5009609E-517.58285533
TACTGAC902.1944896E-417.1302934
AGAGACA2201.8189894E-1216.9742346
GTATAAG2850.016.9567151
Produced by FastQC (version 0.11.5)