FastQCFastQC Report
Wed 25 May 2016
SRR1780277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780277_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645549
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT234313.629623777590857No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC62070.9615071822588216No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG61890.9587188579023435No Hit
CACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTTCTG41030.635583046368285Illumina PCR Primer Index 2 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC13210.2046320263837447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG5800.034.13564744
ATGCCGT34050.032.30327244
GTCTTCT6400.030.9354343
CTTTATA1950.030.464222
CCGTCTT6450.030.35455941
TCTTTAT1950.029.4042571
TTGCGAT300.005744934529.33137116
CGGTATG300.005744934529.33137143
CGTCTTC6700.029.22192642
TATGCCG39850.027.60166543
GAGTTAC407.031308E-427.4981644
TTTATAC3050.025.2481253
GCCGTCT7950.025.18070640
TTGTACT801.3067256E-724.7521784
AGAGTTA450.001398053624.44280843
CTAACGT450.001398053624.44280816
GTATCTC450.001398053624.44280835
TAACCGG551.5932193E-423.99839622
TGTATAA1053.0595402E-923.0496482
TTCCATA49400.022.666529
Produced by FastQC (version 0.11.5)