FastQCFastQC Report
Wed 25 May 2016
SRR1780276_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780276_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81400
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCG150.002217464670.000012
GTATCAT308.310641E-658.33333657
GCCGTAT308.310641E-658.33333654
ATGATAA200.00692896952.51
ACGCACC200.00692896952.523
GTCTAGA200.00692896952.536
AGACGCA200.00692896952.521
CGTATCA352.070263E-550.056
TGCTGCC350.001248908540.02
CCGTATC601.2027425E-535.00000455
CGGTCAG400.002406295635.09
TGGTCGC400.002406295635.049
TAAACAC552.9692566E-431.8181845
AGATCTC703.4515695E-530.040
TAGATCT853.899906E-628.8235339
TGCCCCT500.00717169128.00000238
TTTGGTT500.00717169128.00000233
AATACCA500.00717169128.0000025
CGCCGTA755.5254666E-528.053
ATACCCA808.5739484E-526.254
Produced by FastQC (version 0.11.5)