##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780276_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 81400 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.632076167076168 34.0 31.0 34.0 12.0 34.0 2 29.704471744471743 34.0 31.0 34.0 12.0 34.0 3 29.415442260442262 34.0 30.0 34.0 11.0 34.0 4 29.341584766584766 34.0 27.0 34.0 11.0 34.0 5 29.33566339066339 34.0 27.0 34.0 11.0 34.0 6 32.18199017199017 37.0 31.0 38.0 11.0 38.0 7 32.49416461916462 37.0 31.0 38.0 12.0 38.0 8 32.51379606879607 37.0 31.0 38.0 11.0 38.0 9 32.71636363636364 38.0 33.0 38.0 12.0 38.0 10-11 32.4191339066339 38.0 31.0 38.0 11.0 38.0 12-13 32.47285012285012 38.0 31.0 38.0 11.0 38.0 14-15 32.349508599508596 38.0 31.0 38.0 11.0 38.0 16-17 32.385540540540546 38.0 31.0 38.0 11.0 38.0 18-19 32.41756756756757 38.0 31.0 38.0 11.0 38.0 20-21 32.443789926289924 38.0 31.0 38.0 11.0 38.0 22-23 32.41269041769041 38.0 31.0 38.0 11.0 38.0 24-25 32.4672542997543 38.0 31.0 38.0 11.0 38.0 26-27 32.3802886977887 38.0 31.0 38.0 11.0 38.0 28-29 32.4370945945946 38.0 31.0 38.0 11.0 38.0 30-31 32.46314496314497 38.0 31.5 38.0 11.0 38.0 32-33 32.530798525798524 38.0 32.0 38.0 11.0 38.0 34-35 32.46831081081081 38.0 31.5 38.0 11.0 38.0 36-37 32.40694103194103 38.0 31.0 38.0 11.0 38.0 38-39 32.38138820638821 38.0 31.0 38.0 11.0 38.0 40-41 32.477057739557736 38.0 31.5 38.0 11.0 38.0 42-43 32.548593366093364 38.0 32.0 38.0 11.0 38.0 44-45 32.63704545454546 38.0 33.5 38.0 11.0 38.0 46-47 32.54938574938575 38.0 32.5 38.0 11.0 38.0 48-49 32.46148034398034 38.0 32.0 38.0 11.0 38.0 50-51 32.39218673218673 38.0 31.0 38.0 11.0 38.0 52-53 32.47417076167076 38.0 32.0 38.0 11.0 38.0 54-55 32.46914619164619 38.0 31.5 38.0 11.0 38.0 56-57 32.42644963144963 38.0 31.5 38.0 11.0 38.0 58-59 32.422033169533165 38.0 31.0 38.0 11.0 38.0 60-61 32.46727272727273 38.0 31.5 38.0 11.0 38.0 62-63 32.33451474201475 38.0 31.0 38.0 11.0 38.0 64-65 32.28285012285012 38.0 31.0 38.0 11.0 38.0 66-67 32.20831695331695 37.5 31.0 38.0 11.0 38.0 68-69 32.148513513513514 37.5 31.0 38.0 11.0 38.0 70-71 32.134809582309586 37.5 31.0 38.0 11.0 38.0 72-73 32.00459459459459 38.0 30.5 38.0 11.0 38.0 74-75 32.0362285012285 38.0 30.0 38.0 11.0 38.0 76 30.635454545454547 36.0 25.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.920437268593961 1101 2 1.055686626502684 1101 3 1.041757580537972 1101 4 0.15030272300050385 1101 5 -0.21237317913734088 1101 6 -0.5052135906136179 1101 7 -0.38578310666848026 1101 8 -0.49722236268797815 1101 9 0.13998427797304913 1101 10-11 -0.15714704037304728 1101 12-13 -0.360878023509855 1101 14-15 -0.6529560212758696 1101 16-17 -0.23269446221662093 1101 18-19 -0.25364249365851066 1101 20-21 -0.13495678711079506 1101 22-23 -0.43890161938955075 1101 24-25 -0.6134106679742217 1101 26-27 -0.7451565589111766 1101 28-29 -0.7262709420029481 1101 30-31 -0.13925735236175285 1101 32-33 -0.05275264396968993 1101 34-35 -0.32183432121109234 1101 36-37 -0.26292327608500443 1101 38-39 0.08290417297278907 1101 40-41 -0.4390328234529619 1101 42-43 -0.6402117123399691 1101 44-45 0.0037134750030176633 1101 46-47 0.017455580829263795 1101 48-49 0.06292860464650119 1101 50-51 -0.12003247862704569 1101 52-53 -0.18121492704676 1101 54-55 -0.1961213260211494 1101 56-57 -0.21417318010264808 1101 58-59 -0.20274725540494387 1101 60-61 -0.1810025970090834 1101 62-63 -0.042011569459880604 1101 64-65 0.4543001403382618 1101 66-67 -0.5530865419972741 1101 68-69 -0.1607546582227144 1101 70-71 -0.3011131957734001 1101 72-73 -0.14360130997983234 1101 74-75 -0.21612299672434787 1101 76 -1.162976990991524 1102 1 -0.43064088850346494 1102 2 0.3028133233352648 1102 3 -0.2841700212719829 1102 4 0.05419412571543347 1102 5 -0.5552917311735399 1102 6 0.2546280383456576 1102 7 -0.3305342378901983 1102 8 -0.36032191019928916 1102 9 -0.4995406089047769 1102 10-11 -0.40371943856309045 1102 12-13 -0.429463996360532 1102 14-15 -0.1428315868867287 1102 16-17 -0.3231921997731817 1102 18-19 -0.33590493709742475 1102 20-21 0.00582375587562467 1102 22-23 -0.10826813522665191 1102 24-25 -0.32922514761223454 1102 26-27 -0.26574479420528974 1102 28-29 -0.7835333854418636 1102 30-31 -0.6175378953481783 1102 32-33 -0.8769042276801002 1102 34-35 -0.3975583031115377 1102 36-37 0.1299160904308394 1102 38-39 -1.178000804402771 1102 40-41 -0.6472115564846384 1102 42-43 -0.7046687259146296 1102 44-45 -0.8979041720558101 1102 46-47 -1.1081665911164436 1102 48-49 -1.1310532958059873 1102 50-51 -0.8523062342831516 1102 52-53 -0.5629230718431373 1102 54-55 -0.9617208735324638 1102 56-57 -1.0745804199216522 1102 58-59 -0.7519214635497384 1102 60-61 -0.6626089318507127 1102 62-63 -0.8084369088263976 1102 64-65 -0.366706646992057 1102 66-67 -0.2856317908660486 1102 68-69 -0.7038768301684151 1102 70-71 -0.6585000736014521 1102 72-73 -0.5515990475363957 1102 74-75 -0.681168245593124 1102 76 -0.7670720136159588 1103 1 -0.3207202874618851 1103 2 -0.17176620368599416 1103 3 -0.40711034399032897 1103 4 -0.2802633147353468 1103 5 -0.10397695272224894 1103 6 -0.77360981702871 1103 7 -0.9795566915741389 1103 8 -0.6535431174049577 1103 9 -1.2450157220269489 1103 10-11 -0.8350243988636095 1103 12-13 -1.0688497216230637 1103 14-15 -0.8871541344834206 1103 16-17 -0.49448691504680653 1103 18-19 -0.5010953238471885 1103 20-21 -0.8314190512617401 1103 22-23 -0.846731808068796 1103 24-25 -0.870674818917621 1103 26-27 -0.5050622192885328 1103 28-29 -0.8072143382293646 1103 30-31 -0.9736913146259063 1103 32-33 -0.6336488703847856 1103 34-35 -0.3073060193242938 1103 36-37 -0.6020270496699069 1103 38-39 -1.1412467704234324 1103 40-41 -0.662098861188813 1103 42-43 -0.030023033094686014 1103 44-45 -1.0330318080158527 1103 46-47 -0.5209878153971559 1103 48-49 -1.0698072444101001 1103 50-51 -1.297815497494966 1103 52-53 -0.9571111534618524 1103 54-55 -0.3209798165871902 1103 56-57 -0.7934184631215118 1103 58-59 -1.1070397082351313 1103 60-61 -0.555153540405307 1103 62-63 -0.7937096826674264 1103 64-65 -0.7042375955107971 1103 66-67 -0.5342657872802903 1103 68-69 -0.7841508846378105 1103 70-71 -0.7680471580375503 1103 72-73 -1.2464786684703988 1103 74-75 -0.7364060155922694 1103 76 -0.600995858916054 1104 1 0.2338650569874332 1104 2 -0.06187151303219807 1104 3 -0.08591683806668016 1104 4 0.6265817927679436 1104 5 -0.3066755047187364 1104 6 0.3569957117119671 1104 7 0.11258898635477976 1104 8 -0.5747805022228611 1104 9 -0.7694343266781125 1104 10-11 0.1654692386967227 1104 12-13 -0.11721523281217827 1104 14-15 0.04617188570087194 1104 16-17 -0.023392136635230543 1104 18-19 -0.29178202854222945 1104 20-21 -0.4561777173433583 1104 22-23 0.03342396200579856 1104 24-25 0.554380029700198 1104 26-27 -0.15097051239954595 1104 28-29 -0.5115035001424815 1104 30-31 -0.06251316631524162 1104 32-33 0.6527706118442609 1104 34-35 0.024561027626120335 1104 36-37 -0.02565583422453699 1104 38-39 -0.3491888502830207 1104 40-41 -0.8523467769413351 1104 42-43 0.41013712486933684 1104 44-45 -0.5037865249969826 1104 46-47 -0.45550953544980644 1104 48-49 0.12316116278603317 1104 50-51 -0.08625340885959787 1104 52-53 0.332215305511383 1104 54-55 0.27963448793233425 1104 56-57 -0.25719643591660457 1104 58-59 0.10765972133924606 1104 60-61 0.2026020541537079 1104 62-63 0.17333726774941738 1104 64-65 0.14552107057082253 1104 66-67 0.404413458002729 1104 68-69 -0.3837779140366706 1104 70-71 -0.2731480794943266 1104 72-73 0.2063405504852831 1104 74-75 -0.03065788044527551 1104 76 0.2459764973805676 1105 1 -0.15290205732635798 1105 2 0.36129695514118154 1105 3 0.06715664157083623 1105 4 0.33002103285965845 1105 5 0.10168785372650646 1105 6 0.28128875680422283 1105 7 0.33313708112494567 1105 8 -0.22485147066920064 1105 9 -0.3025274591161491 1105 10-11 -0.2620883549270374 1105 12-13 -0.5866878826647834 1105 14-15 0.1397082979729518 1105 16-17 -0.0777648847518364 1105 18-19 -0.20922934811860472 1105 20-21 0.011580771574649873 1105 22-23 -0.2571645301876728 1105 24-25 -0.3544200576455836 1105 26-27 0.15320024860056236 1105 28-29 -0.6427732894207878 1105 30-31 -0.6514639251317078 1105 32-33 -0.23602729185702032 1105 34-35 -0.4037826780186009 1105 36-37 -0.3494138864136431 1105 38-39 0.11351450161129151 1105 40-41 -0.03362202533089942 1105 42-43 -0.1800473930911437 1105 44-45 -0.1718616423678796 1105 46-47 -0.6593636679970274 1105 48-49 -0.5385267944145298 1105 50-51 -0.2696686288617869 1105 52-53 -0.252564692304972 1105 54-55 -0.2253114668662235 1105 56-57 -0.45102764019654273 1105 58-59 -0.19207824132043072 1105 60-61 -0.29393686930955454 1105 62-63 0.02071143523495067 1105 64-65 -0.607683427736859 1105 66-67 -0.2270419410583031 1105 68-69 -0.6306607615090982 1105 70-71 -0.016242303754616216 1105 72-73 -0.08551445551974268 1105 74-75 0.09807888120992558 1105 76 0.23014507473617485 1106 1 0.47502144700236926 1106 2 0.3890352503558958 1106 3 0.5521245530150374 1106 4 0.8983761174959142 1106 5 0.26684700434889663 1106 6 0.7133643910377572 1106 7 0.06458386580858644 1106 8 0.3917225914404625 1106 9 0.24810354402809764 1106 10-11 0.48705020733337534 1106 12-13 0.48942014162776104 1106 14-15 0.5203237952378856 1106 16-17 1.0081312258567792 1106 18-19 0.5379171393690143 1106 20-21 0.7206624789442486 1106 22-23 0.8801809494177917 1106 24-25 0.880580157713851 1106 26-27 0.7507150007218542 1106 28-29 0.8632244708410894 1106 30-31 0.19973347332631874 1106 32-33 0.3349767138284747 1106 34-35 0.8404133852109297 1106 36-37 0.6013657147406803 1106 38-39 0.7031794023305906 1106 40-41 0.6667699288406155 1106 42-43 0.6111185628893878 1106 44-45 1.382452007113109 1106 46-47 0.6884418193613726 1106 48-49 1.193846035839158 1106 50-51 1.1171922920151616 1106 52-53 0.5768814032284766 1106 54-55 0.7673878482907739 1106 56-57 1.0945424162276325 1106 58-59 0.8646151299161602 1106 60-61 1.0220707974863288 1106 62-63 0.6871627424667253 1106 64-65 1.2402405073107445 1106 66-67 0.9869822653421778 1106 68-69 0.7876857087497626 1106 70-71 0.8617996482633004 1106 72-73 0.5778895157082395 1106 74-75 0.4492439757527187 1106 76 0.5971606236873725 1107 1 0.26642319652231805 1107 2 -0.2107087223345232 1107 3 -0.5277773031829582 1107 4 -0.4710926258367074 1107 5 -0.664815039602459 1107 6 -0.5918414975903623 1107 7 0.4149145677501238 1107 8 0.2996381024283039 1107 9 0.24917032448468035 1107 10-11 0.3561669131153238 1107 12-13 0.31069174393200427 1107 14-15 0.406637001979945 1107 16-17 0.35102646801593806 1107 18-19 -0.6801541215654865 1107 20-21 -0.28641027548289344 1107 22-23 0.4194704736337087 1107 24-25 -0.06887578425329366 1107 26-27 0.20251785969347225 1107 28-29 0.36291510450868003 1107 30-31 0.08167288019637908 1107 32-33 -0.021647992806897776 1107 34-35 -0.21264827469946113 1107 36-37 -0.13960932259662684 1107 38-39 -0.5189562921434856 1107 40-41 0.243002060267969 1107 42-43 -0.23172334024694408 1107 44-45 -0.08053071104349385 1107 46-47 -0.3136490703335255 1107 48-49 -0.09311790698140499 1107 50-51 -0.1839278274642524 1107 52-53 -0.1654591130932701 1107 54-55 -0.08083062834673882 1107 56-57 0.18001286640897973 1107 58-59 0.3123108841299427 1107 60-61 -0.550886317939316 1107 62-63 0.05705819798198064 1107 64-65 0.017614093826637145 1107 66-67 -0.18860979781122467 1107 68-69 0.5371523185214713 1107 70-71 0.23847982748241492 1107 72-73 0.27843357374110056 1107 74-75 -0.021355554863880855 1107 76 0.004116032264288805 1108 1 -0.2649349854889387 1108 2 0.457444617370264 1108 3 0.6254562106749582 1108 4 1.0299374261968488 1108 5 0.3555948573923402 1108 6 1.1480969116694908 1108 7 0.9210205462995589 1108 8 1.5207913359421568 1108 9 0.5643450085666615 1108 10-11 0.9355127769785554 1108 12-13 0.476370834937633 1108 14-15 0.7135394125140806 1108 16-17 0.8494973186052945 1108 18-19 0.6869054515469699 1108 20-21 0.6522007471357796 1108 22-23 1.0050709833501728 1108 24-25 0.8267491493773775 1108 26-27 1.2436562264769577 1108 28-29 0.9150760899605217 1108 30-31 1.5831170768619884 1108 32-33 0.5597473560303072 1108 34-35 0.8052661354099158 1108 36-37 0.6885949887551845 1108 38-39 1.0840913875846638 1108 40-41 1.0390836149032054 1108 42-43 0.6746056392582105 1108 44-45 0.6656198676970817 1108 46-47 0.890800329687707 1108 48-49 0.876033627477554 1108 50-51 1.0992940053912221 1108 52-53 0.339595575236352 1108 54-55 1.5289814137048765 1108 56-57 1.046785724006945 1108 58-59 0.5194787719923113 1108 60-61 0.39449968609594066 1108 62-63 0.8241071520013108 1108 64-65 0.4028960307492184 1108 66-67 0.8998472479570623 1108 68-69 0.6055923737407554 1108 70-71 0.592414201486875 1108 72-73 0.4201772288329515 1108 74-75 1.0271418434583062 1108 76 0.386360908551854 1109 1 -0.799736334014522 1109 2 -1.1293133734973146 1109 3 -0.8368405503031475 1109 4 -0.8504449405509007 1109 5 -0.6151301884831355 1109 6 -0.5615220018285711 1109 7 -1.1094279664815616 1109 8 -1.0711943253047984 1109 9 -0.3688474977278844 1109 10-11 -0.6734788160739811 1109 12-13 -0.7398733506126618 1109 14-15 -0.7215307876310106 1109 16-17 -0.9429748360483963 1109 18-19 -1.202801372163183 1109 20-21 -0.9009614600079949 1109 22-23 -1.0095558249970296 1109 24-25 -0.9528031913387096 1109 26-27 -0.4166518860139732 1109 28-29 -0.8723924373300491 1109 30-31 -0.8556124925486728 1109 32-33 -0.34445825144632636 1109 34-35 -0.5881427324260429 1109 36-37 -0.9169186031878063 1109 38-39 -0.49391825693374614 1109 40-41 -0.756813197284739 1109 42-43 -0.9314733945829587 1109 44-45 -0.9145341885483873 1109 46-47 -1.1399042322548496 1109 48-49 -0.4304358813348088 1109 50-51 -0.8889810767578865 1109 52-53 -0.5569392261121777 1109 54-55 -0.2169858120024557 1109 56-57 -0.9062292548690039 1109 58-59 -1.0193126759656899 1109 60-61 -0.6356287652333812 1109 62-63 -0.8570582984318449 1109 64-65 -0.6709101400355735 1109 66-67 -0.4133435513430648 1109 68-69 -0.8641191442040252 1109 70-71 -0.35323936399769806 1109 72-73 -0.6760545810078717 1109 74-75 -0.2621510341075215 1109 76 -0.2362012900569468 1110 1 0.11176652287523225 1110 2 0.20954918574439318 1110 3 0.25630734357114804 1110 4 -0.7067588883739049 1110 5 0.20025715261621357 1110 6 0.227843281424299 1110 7 0.8611837179760684 1110 8 -0.07681951908607942 1110 9 0.32962882773608726 1110 10-11 -0.14087926786119453 1110 12-13 0.446787853267395 1110 14-15 0.1331221777762579 1110 16-17 -0.08103569444410397 1110 18-19 0.21612053951684373 1110 20-21 0.24650055886076672 1110 22-23 -0.07932815967391349 1110 24-25 0.1318736922153505 1110 26-27 0.18588609511726162 1110 28-29 0.06707029022453526 1110 30-31 -0.13783555141388604 1110 32-33 -0.4118166250123494 1110 34-35 -0.1293461695523206 1110 36-37 0.2543397570903494 1110 38-39 -0.3462901398234095 1110 40-41 0.04706907228163004 1110 42-43 0.5029399464278015 1110 44-45 0.10711394893666437 1110 46-47 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-1.182829272625387 2116 26-27 -1.704458884492567 2116 28-29 -1.2510383838634098 2116 30-31 -1.5439085151524523 2116 32-33 -1.8332758997836436 2116 34-35 -2.0335785072576016 2116 36-37 -1.8163535086431395 2116 38-39 -1.256165594469067 2116 40-41 -1.8430444513599404 2116 42-43 -1.5526535728050845 2116 44-45 -1.3944841994155865 2116 46-47 -1.2276025587056232 2116 48-49 -1.3814900000046606 2116 50-51 -1.5164859669991344 2116 52-53 -2.1515056247211746 2116 54-55 -1.8203655120676636 2116 56-57 -1.3106848080096256 2116 58-59 -1.1225728368002912 2116 60-61 -1.5950723644509424 2116 62-63 -1.594104592715695 2116 64-65 -2.198664975940808 2116 66-67 -1.4234935187414557 2116 68-69 -1.6023825651994592 2116 70-71 -1.1057062190292157 2116 72-73 -1.6331943332356467 2116 74-75 -1.7143788106778324 2116 76 -1.665651409596176 2117 1 -0.4572106676087415 2117 2 -0.45689958039386624 2117 3 -0.3439074440925687 2117 4 -0.18624900113742981 2117 5 0.9538583479562526 2117 6 0.2547617788445109 2117 7 0.7473696026911298 2117 8 0.032161873765225835 2117 9 0.061634524278467495 2117 10-11 0.2939490187402498 2117 12-13 -0.12453565897783392 2117 14-15 0.2778937324187041 2117 16-17 -0.1809703242855889 2117 18-19 0.3166038117602099 2117 20-21 -0.05324496445069826 2117 22-23 -0.03983757998068427 2117 24-25 0.28124450443957016 2117 26-27 0.36247890906912517 2117 28-29 0.16207881169162874 2117 30-31 0.10162934714563221 2117 32-33 0.21491976982341043 2117 34-35 0.05449572804999292 2117 36-37 0.08142401455538817 2117 38-39 0.5145297887363398 2117 40-41 0.7305607726061538 2117 42-43 0.27587203150239503 2117 44-45 0.4725681548059768 2117 46-47 0.6675171571839371 2117 48-49 0.29982515637064466 2117 50-51 0.26643303861433765 2117 52-53 0.19256586113058205 2117 54-55 0.7238663587079159 2117 56-57 0.12843765733577328 2117 58-59 0.12704338498915035 2117 60-61 0.36724863451800616 2117 62-63 0.31838251921006844 2117 64-65 0.2066277265451575 2117 66-67 0.06451198017021653 2117 68-69 -0.47499111142468564 2117 70-71 -0.04001713666010431 2117 72-73 -0.4118402262359915 2117 74-75 0.26996074711801654 2117 76 -0.957139552567881 2118 1 1.0320393669200563 2118 2 0.39405111248399294 2118 3 0.4668977674538617 2118 4 0.20562982580424105 2118 5 -0.33475635670743387 2118 6 0.8403471570499335 2118 7 0.4840299774436687 2118 8 0.7512355812372533 2118 9 0.13582539946838068 2118 10-11 0.07418473532788994 2118 12-13 0.47741636901351114 2118 14-15 0.04949724975216441 2118 16-17 0.27197843497963703 2118 18-19 0.6406565717620509 2118 20-21 0.12707592316957061 2118 22-23 0.2848366983674566 2118 24-25 0.3252341918388595 2118 26-27 0.4992359644533124 2118 28-29 0.8332150393054647 2118 30-31 0.5485931149279679 2118 32-33 0.6064763279929224 2118 34-35 0.43989465075152623 2118 36-37 0.06906270522340208 2118 38-39 0.2771097804494289 2118 40-41 0.2548690457059166 2118 42-43 -0.11371638523716143 2118 44-45 -0.08042203901568001 2118 46-47 0.504955580829261 2118 48-49 -0.3369779374095714 2118 50-51 -0.15066331974853853 2118 52-53 -0.019556048542078486 2118 54-55 -0.15857459704918853 2118 56-57 0.1545184086823994 2118 58-59 -0.08006033951708957 2118 60-61 -0.06553530728945134 2118 62-63 0.36023142119432094 2118 64-65 -0.31576995311968403 2118 66-67 0.0422639252924526 2118 68-69 0.43961917355299107 2118 70-71 0.49722792572192276 2118 72-73 0.3192725217958703 2118 74-75 -0.04252486588322313 2118 76 -0.035266710617694486 2119 1 -0.21269217055474599 2119 2 0.49323564611052717 2119 3 -1.368634576324773 2119 4 -0.059305120136752265 2119 5 0.9526268208626583 2119 6 0.36096287997461474 2119 7 0.02617767764524359 2119 8 -0.17065373523699634 2119 9 0.8824352583652058 2119 10-11 1.1121176655093024 2119 12-13 0.6610337411960288 2119 14-15 0.5306224100966759 2119 16-17 0.5859329887637728 2119 18-19 0.45312221222383897 2119 20-21 0.09764125210489283 2119 22-23 0.24580426296338942 2119 24-25 0.04992266535910517 2119 26-27 0.40827481363784557 2119 28-29 0.27510160701666564 2119 30-31 -0.12847303863626536 2119 32-33 0.3759238266185392 2119 34-35 -0.06761863493657927 2119 36-37 0.7045767239149967 2119 38-39 0.08368848669828566 2119 40-41 0.17405541184115236 2119 42-43 0.07851377785610936 2119 44-45 0.26386053382654495 2119 46-47 0.2451516592606282 2119 48-49 0.2154776242543477 2119 50-51 0.22933026647099553 2119 52-53 0.20932428863951458 2119 54-55 -0.07352328680546094 2119 56-57 -0.06760455265166598 2119 58-59 0.24543901910486454 2119 60-61 0.1893895598536588 2119 62-63 0.4181845089714855 2119 64-65 0.7588589638676737 2119 66-67 0.3480409089831227 2119 68-69 0.10532377314983421 2119 70-71 -0.023319078126341708 2119 72-73 -0.13266993743081557 2119 74-75 0.17064170915800858 2119 76 0.4614347737143589 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 21.0 13 231.0 14 886.0 15 1566.0 16 2175.0 17 2491.0 18 2473.0 19 2308.0 20 1769.0 21 1230.0 22 928.0 23 731.0 24 606.0 25 584.0 26 682.0 27 772.0 28 899.0 29 1063.0 30 1261.0 31 1516.0 32 1936.0 33 2467.0 34 3642.0 35 5284.0 36 10026.0 37 33852.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.522113022113025 19.706388206388205 11.737100737100738 33.03439803439803 2 18.664619164619165 18.964373464373466 34.6990171990172 27.671990171990174 3 19.99140049140049 21.111793611793612 27.703931203931205 31.192874692874696 4 14.957002457002458 18.554054054054053 31.91154791154791 34.57739557739558 5 15.58108108108108 30.778869778869776 34.056511056511056 19.583538083538084 6 28.832923832923836 33.004914004914006 17.371007371007373 20.791154791154792 7 26.6953316953317 28.740786240786242 19.816953316953317 24.74692874692875 8 23.21990171990172 29.998771498771497 19.065110565110565 27.716216216216218 9 23.37006597132643 17.253283209867444 19.965847246280667 39.41080357252546 10-11 25.697174447174447 22.853194103194102 24.37714987714988 27.07248157248157 12-13 24.49570024570025 20.171375921375923 24.32125307125307 31.01167076167076 14-15 23.877764127764127 21.22420147420147 22.716216216216218 32.18181818181819 16-17 25.275184275184277 22.822481572481575 22.885135135135133 29.01719901719902 18-19 24.288083538083537 21.775798525798525 23.565724815724813 30.370393120393118 20-21 24.004299754299755 22.307125307125308 24.08845208845209 29.60012285012285 22-23 26.11732186732187 22.113636363636363 22.553439803439804 29.215601965601966 24-25 24.470515970515972 23.68058968058968 22.222358722358724 29.626535626535627 26-27 23.665233415233416 23.73832923832924 23.85135135135135 28.745085995085994 28-29 24.28869778869779 22.783783783783782 25.24570024570025 27.68181818181818 30-31 24.235872235872236 21.95823095823096 25.38022113022113 28.425675675675677 32-33 23.37837837837838 23.54361179361179 25.07248157248157 28.005528255528255 34-35 23.515356265356267 23.22972972972973 24.987100737100736 28.267813267813267 36-37 24.175675675675677 21.805896805896808 25.28931203931204 28.72911547911548 38-39 22.638206388206388 21.7997542997543 27.5982800982801 27.963759213759214 40-41 22.60933660933661 21.354422604422606 27.890663390663388 28.145577395577398 42-43 20.925061425061426 21.54176904176904 28.02149877149877 29.51167076167076 44-45 20.28194103194103 21.286240786240786 29.6504914004914 28.781326781326783 46-47 20.535626535626538 22.291154791154792 29.453931203931205 27.71928746928747 48-49 20.201474201474202 22.71805896805897 28.726044226044227 28.354422604422602 50-51 19.880835380835382 24.011056511056513 27.19041769041769 28.917690417690416 52-53 19.005528255528255 24.019041769041767 27.904791154791152 29.070638820638823 54-55 19.054054054054053 23.484643734643733 27.81326781326781 29.6480343980344 56-57 19.544226044226043 23.355651105651106 27.28009828009828 29.82002457002457 58-59 19.896191646191646 23.59152334152334 26.481572481572478 30.030712530712535 60-61 19.818181818181817 22.532555282555283 26.96928746928747 30.679975429975432 62-63 19.71253071253071 22.983415233415233 26.589066339066342 30.714987714987714 64-65 20.61117936117936 23.10687960687961 25.479729729729726 30.8022113022113 66-67 20.165847665847668 24.455773955773953 24.07002457002457 31.308353808353807 68-69 20.15909090909091 25.49078624078624 24.00184275184275 30.3482800982801 70-71 20.23710073710074 26.087837837837842 24.619164619164618 29.055896805896808 72-73 20.504299754299755 25.027027027027028 26.07063882063882 28.3980343980344 74-75 19.81203931203931 26.515356265356267 25.46068796068796 28.211916461916463 76 19.249385749385752 26.35995085995086 24.39189189189189 29.998771498771497 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 0.5 12 1.0 13 1.0 14 1.5 15 3.5 16 5.5 17 6.0 18 10.0 19 18.0 20 29.0 21 36.0 22 42.0 23 52.0 24 72.0 25 88.0 26 144.0 27 221.5 28 311.5 29 380.0 30 443.5 31 590.5 32 818.0 33 962.0 34 1111.5 35 1431.5 36 1700.5 37 1799.0 38 2074.5 39 2600.5 40 3166.0 41 3602.5 42 3724.0 43 3797.0 44 4020.5 45 4195.5 46 4220.0 47 4227.5 48 4055.5 49 3708.5 50 3541.0 51 3312.0 52 2760.0 53 2361.0 54 2285.0 55 2204.0 56 2063.0 57 2011.5 58 2020.0 59 2016.5 60 2029.0 61 2084.5 62 2124.0 63 2111.0 64 2112.0 65 2108.5 66 2084.5 67 2078.0 68 1997.0 69 1863.5 70 1673.5 71 1536.0 72 1391.0 73 1079.5 74 761.0 75 609.0 76 454.5 77 223.0 78 100.0 79 54.0 80 39.5 81 17.5 82 8.5 83 7.0 84 6.5 85 7.0 86 5.5 87 3.0 88 4.0 89 4.5 90 3.5 91 4.5 92 6.0 93 4.0 94 1.5 95 1.5 96 2.0 97 2.5 98 4.5 99 15.5 100 25.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0012285012285012285 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 81400.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.87837837837839 #Duplication Level Percentage of deduplicated Percentage of total 1 79.48869052777538 56.34029484029484 2 11.94557587312592 16.93366093366093 3 4.225669468758125 8.985257985257986 4 1.821648323078256 5.164619164619165 5 0.9082242828667996 3.218673218673219 6 0.5667735505676402 2.4103194103194103 7 0.29638616864546324 1.4705159705159705 8 0.23745558540601439 1.3464373464373465 9 0.1542594678914984 0.984029484029484 >10 0.355316751884912 3.1461916461916464 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0012285012285012285 0.0 2 0.0 0.0 0.0 0.007371007371007371 0.0 3 0.0 0.0 0.0 0.012285012285012284 0.0 4 0.0 0.0 0.0 0.03562653562653563 0.0 5 0.0 0.0 0.0 0.04176904176904177 0.0 6 0.0 0.0 0.0 0.04176904176904177 0.0 7 0.0 0.0 0.0 0.044226044226044224 0.0 8 0.0 0.0 0.0 0.044226044226044224 0.0 9 0.0 0.0 0.0 0.04791154791154791 0.0 10 0.0 0.0 0.0 0.051597051597051594 0.0 11 0.0 0.0 0.0 0.05405405405405406 0.0 12 0.0 0.0 0.0 0.05405405405405406 0.0 13 0.0 0.0 0.0 0.056511056511056514 0.0 14 0.0 0.0 0.0 0.056511056511056514 0.0 15 0.0 0.0 0.0 0.056511056511056514 0.0 16 0.0 0.0 0.0 0.056511056511056514 0.0 17 0.0 0.0 0.0 0.06388206388206388 0.0 18 0.0 0.0 0.0 0.07248157248157248 0.0 19 0.0 0.0 0.0 0.08476658476658476 0.0 20 0.0 0.0 0.0 0.08968058968058969 0.0 21 0.0 0.0 0.0 0.09336609336609336 0.0 22 0.0 0.0 0.0 0.10319410319410319 0.0 23 0.0 0.0 0.0 0.11425061425061425 0.0 24 0.0 0.0 0.0 0.12039312039312039 0.0 25 0.0 0.0 0.0 0.12530712530712532 0.0 26 0.0 0.0 0.0 0.14250614250614252 0.0 27 0.0 0.0 0.0 0.22972972972972974 0.0 28 0.0 0.0 0.0 0.33906633906633904 0.0 29 0.0 0.0 0.0 0.48034398034398035 0.0 30 0.0 0.0 0.0 0.6117936117936118 0.0 31 0.0 0.0 0.0 0.7800982800982801 0.0 32 0.0 0.0 0.0 0.9238329238329238 0.0 33 0.0 0.0 0.0 1.1474201474201475 0.0 34 0.0 0.0 0.0 1.5331695331695332 0.0 35 0.0 0.0 0.0 2.017199017199017 0.0 36 0.0 0.0 0.0 2.6474201474201475 0.0 37 0.0 0.0 0.0 3.4692874692874693 0.0 38 0.0 0.0 0.0 4.302211302211302 0.0 39 0.0 0.0 0.0 5.216216216216216 0.0 40 0.0 0.0 0.0 6.094594594594595 0.0 41 0.0 0.0 0.0 7.015970515970516 0.0 42 0.0 0.0 0.0 7.953316953316953 0.0 43 0.0 0.0 0.0 8.92137592137592 0.0 44 0.0 0.0 0.0 9.834152334152334 0.0 45 0.0 0.0 0.0 10.831695331695332 0.0 46 0.0 0.0 0.0 11.85135135135135 0.0 47 0.0 0.0 0.0 12.743243243243244 0.0 48 0.0 0.0 0.0 13.51105651105651 0.0 49 0.0 0.0 0.0 14.33046683046683 0.0 50 0.0 0.0 0.0 15.0995085995086 0.0 51 0.0 0.0 0.0 15.872235872235873 0.0 52 0.0 0.0 0.0 16.673218673218674 0.0 53 0.0 0.0 0.0 17.42014742014742 0.0 54 0.0 0.0 0.0 18.168304668304668 0.0 55 0.0 0.0 0.0 18.8022113022113 0.0 56 0.0 0.0 0.0 19.4987714987715 0.0 57 0.0 0.0 0.0 20.127764127764127 0.0 58 0.0 0.0 0.0 20.765356265356264 0.0 59 0.0 0.0 0.0 21.355036855036854 0.0 60 0.0 0.0 0.0 22.0012285012285 0.0 61 0.0 0.0 0.0 22.601965601965603 0.0 62 0.0 0.0 0.0 23.273955773955773 0.0 63 0.0 0.0 0.0 23.818181818181817 0.0 64 0.0 0.0 0.0 24.512285012285012 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGCG 15 0.0022174646 70.00001 2 GTATCAT 30 8.310641E-6 58.333336 57 GCCGTAT 30 8.310641E-6 58.333336 54 ATGATAA 20 0.006928969 52.5 1 ACGCACC 20 0.006928969 52.5 23 GTCTAGA 20 0.006928969 52.5 36 AGACGCA 20 0.006928969 52.5 21 CGTATCA 35 2.070263E-5 50.0 56 TGCTGCC 35 0.0012489085 40.0 2 CCGTATC 60 1.2027425E-5 35.000004 55 CGGTCAG 40 0.0024062956 35.0 9 TGGTCGC 40 0.0024062956 35.0 49 TAAACAC 55 2.9692566E-4 31.818184 5 AGATCTC 70 3.4515695E-5 30.0 40 TAGATCT 85 3.899906E-6 28.82353 39 TGCCCCT 50 0.007171691 28.000002 38 TTTGGTT 50 0.007171691 28.000002 33 AATACCA 50 0.007171691 28.000002 5 CGCCGTA 75 5.5254666E-5 28.0 53 ATACCCA 80 8.5739484E-5 26.25 4 >>END_MODULE