FastQCFastQC Report
Wed 25 May 2016
SRR1780276_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780276_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81400
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT77229.486486486486486No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC21902.6904176904176906No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG19202.3587223587223587No Hit
CACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTG16852.07002457002457Illumina PCR Primer Index 9 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC4610.5663390663390664No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT2440.29975429975429974No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT2290.28132678132678135Illumina PCR Primer Index 9 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1340.16461916461916462No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT1320.16216216216216214No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC1260.1547911547911548No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1100.13513513513513514No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG150.002217464670.000011
GAAGACG150.002217464670.000014
TACATGC150.002217464670.000012
GCGTCAA200.00692896952.511
TAAGACT200.00692896952.59
AAAGCGA200.00692896952.525
TTAATAC404.5571025E-543.752
TCTTTAT404.5571025E-543.751
CTTGAAA16550.036.586157
GTCTTCT16650.036.57657649
CTCGTAT16850.036.5578639
CGTCTTC16850.036.5578648
CCGTCTT16850.036.5578647
TATGCCG17000.036.44117743
TGCCGTC17000.036.44117745
CGTATGC17050.036.33431241
TCGTATG17050.036.33431240
TTGAAAA16700.036.25748458
CTGCTTG16900.036.24260354
GTATGCC17100.036.22807342
Produced by FastQC (version 0.11.5)