FastQCFastQC Report
Wed 25 May 2016
SRR1780275_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780275_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458815
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTGAC300.005740434429.33427810
TATGGTC407.0690265E-427.4709137
CATCGTT609.791154E-625.64790532
ACGAGAG801.3015779E-724.75890224
GAGCTAT450.001386006624.47728215
TCTAGTT450.001402855724.42657533
AAGTTGT500.002557995322.02955219
TCTCTAA703.187376E-522.0199337
TCTAGCC602.8917528E-421.9743353
CATAGGT500.002595552221.9743351
TAGATCT1750.021.39078939
GACGAAA1551.8189894E-1221.29565623
AGCTATG550.00444476620.02686516
AGCTAGT550.00450696219.9788466
TGCGTGT809.023606E-519.2359333
ATGGTCT708.1707595E-418.8371968
AAACTCG600.00743188618.3219327
CGAAACT600.00743188618.3219325
GCTAGTC600.007450605318.3139427
TAATACC600.00745529118.3119454
Produced by FastQC (version 0.11.5)