FastQCFastQC Report
Wed 25 May 2016
SRR1780275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780275_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458815
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT217114.73197258154158No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC60931.327986225384959No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG55531.2102917297821563No Hit
CACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCTG43590.950056122838181RNA PCR Primer, Index 36 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC12570.2739666314309689No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT5530.12052788160805553RNA PCR Primer, Index 15 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATCG207.8536384E-443.997611
CTAAAGC250.002350647535.201923
AACGTTA250.002351904835.1980828
GATTTAA250.002351904835.19808232
CGTTATC250.002351904835.19808210
TATCGTC250.002351904835.19808213
AATACAG250.002351904835.1980825
TCTTCTG6650.034.4041444
GTCTTCT6750.033.89444743
CCGTCTT7050.032.14009541
CTTTATA1950.031.5914652
ATGCCGT30000.031.45828644
CGTCTTC7250.031.2534742
ATATAAC300.005706259529.3701441
TCTTTAT2100.029.3701441
CCTTATA454.0625964E-529.3349322
ACGTTAT300.00574287629.3317369
GCCGTCT7850.029.14490940
AGCGCTT407.0276763E-427.498518
TTTATAC2550.026.7465573
Produced by FastQC (version 0.11.5)