FastQCFastQC Report
Wed 25 May 2016
SRR1780274_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780274_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134876
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGGA150.002220636270.000012
ACGCTTC252.3834499E-456.00000415
CCGACAA200.006938804452.521
GACGCTT200.006938804452.514
TCGCGAA350.00125183240.011
CTTCCGA400.00241189435.018
CCTTGCG400.00241189435.054
TTCTAAG450.004295117731.1111132
AGGAGAT450.004295117731.11111330
TTGCTAG450.004295117731.11111328
TGGAAGG450.004295117731.1111135
ACACTTT450.004295117731.11111339
GGACTCG500.00718817328.0000021
CTTGCGG500.00718817328.00000255
ATTGCTA500.00718817328.00000227
CTGGAAG500.00718817328.0000024
TAGATCT755.5521064E-528.039
AGATCTC1003.908852E-421.00000240
GGTCTAG1055.4322736E-420.0000021
ATCCACA1000.00964739717.5000024
Produced by FastQC (version 0.11.5)