##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780274_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 134876 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.619005605148434 34.0 32.0 34.0 21.0 34.0 2 30.66248999080637 34.0 32.0 34.0 12.0 34.0 3 30.526416856964914 34.0 32.0 34.0 11.0 34.0 4 30.30289302766986 34.0 32.0 34.0 11.0 34.0 5 30.421728105815713 34.0 32.0 34.0 11.0 34.0 6 33.43556303567721 38.0 34.0 38.0 21.0 38.0 7 33.81405142501261 38.0 34.0 38.0 21.0 38.0 8 33.74040600255049 38.0 34.0 38.0 21.0 38.0 9 33.995966665678104 38.0 35.0 38.0 21.0 38.0 10-11 33.774478039087754 38.0 34.0 38.0 21.0 38.0 12-13 33.85621237284617 38.0 34.0 38.0 21.0 38.0 14-15 33.76637429935644 38.0 34.0 38.0 21.0 38.0 16-17 33.842522020225985 38.0 34.0 38.0 21.0 38.0 18-19 33.833250541237874 38.0 34.0 38.0 21.0 38.0 20-21 33.85481108573801 38.0 34.0 38.0 21.0 38.0 22-23 33.83856282807912 38.0 34.5 38.0 21.0 38.0 24-25 33.86507977698034 38.0 34.5 38.0 21.0 38.0 26-27 33.83499658945995 38.0 34.5 38.0 21.0 38.0 28-29 33.927351789792105 38.0 35.0 38.0 21.0 38.0 30-31 33.935192324802046 38.0 35.0 38.0 21.0 38.0 32-33 34.00684702986447 38.0 35.0 38.0 21.0 38.0 34-35 33.94467140188024 38.0 35.0 38.0 21.0 38.0 36-37 33.89765043447315 38.0 35.0 38.0 21.0 38.0 38-39 33.91060677956048 38.0 35.0 38.0 21.0 38.0 40-41 33.94442302559388 38.0 35.0 38.0 21.0 38.0 42-43 34.014346510869245 38.0 35.0 38.0 21.0 38.0 44-45 34.08410317625078 38.0 35.5 38.0 21.0 38.0 46-47 34.01972181855927 38.0 35.5 38.0 21.0 38.0 48-49 33.96863415285151 38.0 35.0 38.0 21.0 38.0 50-51 33.89801373113082 38.0 35.0 38.0 21.0 38.0 52-53 33.96453779768083 38.0 35.0 38.0 21.0 38.0 54-55 33.96723286574335 38.0 35.0 38.0 21.0 38.0 56-57 33.92027862629378 38.0 35.0 38.0 21.0 38.0 58-59 33.98029300987574 38.0 35.0 38.0 21.0 38.0 60-61 33.973375544944986 38.0 35.0 38.0 21.0 38.0 62-63 33.85151546605771 38.0 35.0 38.0 20.5 38.0 64-65 33.80447596310685 38.0 35.0 38.0 20.0 38.0 66-67 33.72697144043418 38.0 34.5 38.0 20.0 38.0 68-69 33.69145363148373 38.0 34.0 38.0 19.5 38.0 70-71 33.64571161659599 38.0 34.0 38.0 19.0 38.0 72-73 33.58863326314541 38.0 34.0 38.0 19.0 38.0 74-75 33.57133589370978 38.0 34.0 38.0 19.0 38.0 76 32.09237373587592 37.0 30.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.34176278308919095 1101 2 -0.2413479725732799 1101 3 0.04571575691885954 1101 4 -0.0630548689592132 1101 5 -0.1826239956744402 1101 6 -0.3851935002544451 1101 7 -0.4105833281798468 1101 8 -0.6823790502824778 1101 9 -0.6292220276694565 1101 10-11 -0.6959524357796667 1101 12-13 -0.8090026047590229 1101 14-15 -0.9671547152123949 1101 16-17 -0.2506543881714691 1101 18-19 -0.857583879557339 1101 20-21 -0.6100644637215638 1101 22-23 -0.7981399087779124 1101 24-25 -0.7231497672474774 1101 26-27 -0.6845378474492776 1101 28-29 -1.3317382616827942 1101 30-31 -1.1347783286270428 1101 32-33 -0.7545367332927739 1101 34-35 -0.6912838530140775 1101 36-37 -1.309732807861451 1101 38-39 -0.6808758850195886 1101 40-41 -0.9543819813229106 1101 42-43 -0.9896584163969138 1101 44-45 -0.9494499304579591 1101 46-47 -0.5457340578942222 1101 48-49 -0.8597896992909853 1101 50-51 -0.7428244382483484 1101 52-53 -0.6141400409317583 1101 54-55 -1.1729626088124192 1101 56-57 -1.181174996674038 1101 58-59 -1.0426131859166006 1101 60-61 -0.9450270217068351 1101 62-63 -1.0105426541305604 1101 64-65 -0.9899074370693342 1101 66-67 -1.102170101433174 1101 68-69 -1.122317667574606 1101 70-71 -1.056668651345909 1101 72-73 -1.024315143020047 1101 74-75 -1.3126784052479366 1101 76 -1.9112229337288476 1102 1 -0.2982028729034383 1102 2 -0.12679398196295466 1102 3 -0.8435677703315996 1102 4 0.22908638435778883 1102 5 -0.12473463049787625 1102 6 -0.39413410037691676 1102 7 -0.613032807665455 1102 8 -0.7018524121930767 1102 9 -0.581089755777235 1102 10-11 -0.33689956784743913 1102 12-13 -0.07805751392415772 1102 14-15 -0.2096327719586597 1102 16-17 -0.12013795624659451 1102 18-19 -0.3460509136459251 1102 20-21 -0.225475772151853 1102 22-23 -0.3794626277001356 1102 24-25 -0.5145561767187985 1102 26-27 -0.015809535548896747 1102 28-29 -0.31067273810655394 1102 30-31 -0.04245336434861713 1102 32-33 -0.05002560857344207 1102 34-35 0.22990414453644092 1102 36-37 -0.10991651882287101 1102 38-39 -0.3351522679548822 1102 40-41 -0.527151934374551 1102 42-43 -0.49676190690517785 1102 44-45 -0.2923362337851714 1102 46-47 -0.589620565344724 1102 48-49 -0.5505225019037709 1102 50-51 -0.4206158242454876 1102 52-53 -0.24655481945798385 1102 54-55 -0.7928850419620375 1102 56-57 -0.16119132653727064 1102 58-59 -0.23018207752713238 1102 60-61 -0.40224278002485647 1102 62-63 -0.5316081777067936 1102 64-65 -0.2589215215763687 1102 66-67 -0.7830641614454166 1102 68-69 -0.23979062123861894 1102 70-71 -0.5553010252997765 1102 72-73 -0.27777503279346405 1102 74-75 -0.42982476164719685 1102 76 -0.18074732646206115 1103 1 0.4632542551339043 1103 2 0.20265580457497023 1103 3 0.13535733839441022 1103 4 0.17538172011169095 1103 5 0.06734931795202925 1103 6 -0.566890753610771 1103 7 0.3885791302509958 1103 8 0.25379535543250853 1103 9 0.19760393611918659 1103 10-11 0.08951786023379782 1103 12-13 -0.20931873564542514 1103 14-15 0.016234043665960485 1103 16-17 0.2894778120173811 1103 18-19 0.18823867658669258 1103 20-21 0.1537406025838166 1103 22-23 0.20697155815152257 1103 24-25 0.036171988305341074 1103 26-27 -0.06323801577870825 1103 28-29 0.40560623887146363 1103 30-31 0.0012564868264206552 1103 32-33 0.020764001608903015 1103 34-35 0.39148496087926077 1103 36-37 -0.0710704110145457 1103 38-39 -0.41661099197035867 1103 40-41 -0.10244619284321033 1103 42-43 0.058734242797576997 1103 44-45 0.08771802947141794 1103 46-47 -0.024951531310492214 1103 48-49 0.031518754131028004 1103 50-51 -0.3170248734415253 1103 52-53 0.36516364999899054 1103 54-55 -0.019869042281236204 1103 56-57 0.3261206472886826 1103 58-59 0.12432855567718804 1103 60-61 -0.058764345679406915 1103 62-63 -0.04108828782928242 1103 64-65 0.2525854807776966 1103 66-67 0.3525780202566935 1103 68-69 0.17382307225377502 1103 70-71 0.280975557711038 1103 72-73 0.0403233592585579 1103 74-75 0.5585126281305577 1103 76 0.657205033590607 1104 1 0.326294245307178 1104 2 0.23886882959473965 1104 3 0.15934837150587455 1104 4 0.37191626336080574 1104 5 -0.2728089844808501 1104 6 0.3535127563733411 1104 7 -0.019491069431175845 1104 8 -0.2991576528511146 1104 9 -0.0015561857936461365 1104 10-11 -0.07992792770849633 1104 12-13 -0.00801285573109567 1104 14-15 0.2575300968531238 1104 16-17 0.02712338960630234 1104 18-19 0.02053559728960863 1104 20-21 -0.5386022273600659 1104 22-23 -0.07344331398587656 1104 24-25 -0.09815860429236523 1104 26-27 0.13106248246705832 1104 28-29 0.23424500684554062 1104 30-31 0.15129448862286665 1104 32-33 0.13853502351174996 1104 34-35 0.008315534284136561 1104 36-37 -0.15866647111112542 1104 38-39 0.05586265627886888 1104 40-41 -0.5093955314584093 1104 42-43 -0.37787568987399567 1104 44-45 -0.3942714221511423 1104 46-47 0.1426363910276507 1104 48-49 -0.09448982890098279 1104 50-51 -0.45441864114690134 1104 52-53 -0.38356386560478484 1104 54-55 -0.06692985283267916 1104 56-57 0.09586046933279846 1104 58-59 -0.3412817378195072 1104 60-61 -0.5288964690969564 1104 62-63 -0.229642453824205 1104 64-65 -0.07175025549751268 1104 66-67 0.11755418276619167 1104 68-69 0.02162011962935395 1104 70-71 0.08442949876603478 1104 72-73 -0.1111656202204685 1104 74-75 0.046647622794957044 1104 76 0.015512072751647565 1105 1 0.48075039214137405 1105 2 0.2792959668206585 1105 3 0.16308235770937074 1105 4 0.6994368965216822 1105 5 0.4440393771845841 1105 6 0.8048015590340967 1105 7 0.7473465137173889 1105 8 -0.007206125381294726 1105 9 0.6612918063226374 1105 10-11 0.7086630121781781 1105 12-13 0.0384117562422972 1105 14-15 0.5214651793857641 1105 16-17 0.3071107633436725 1105 18-19 0.7623477739340956 1105 20-21 0.8172659051848967 1105 22-23 0.781096751301142 1105 24-25 0.5603317874297318 1105 26-27 0.8155444977417616 1105 28-29 0.33569009800099536 1105 30-31 0.5495760850752021 1105 32-33 0.26100592151618685 1105 34-35 0.8628786963930395 1105 36-37 0.5081772711899291 1105 38-39 0.8264034298684209 1105 40-41 0.8475119580710313 1105 42-43 0.8942472160141577 1105 44-45 0.5939953923351879 1105 46-47 0.8116358367039282 1105 48-49 0.7092526261372072 1105 50-51 0.9177404497621922 1105 52-53 0.785135321387088 1105 54-55 0.7939387416487165 1105 56-57 0.48023392954467425 1105 58-59 0.7185775255458466 1105 60-61 0.6911431034578612 1105 62-63 0.7311198952765636 1105 64-65 0.4160584720215752 1105 66-67 1.0133066772229498 1105 68-69 0.8653470228074767 1105 70-71 0.5904616187463247 1105 72-73 0.8619496791143391 1105 74-75 1.0433986698508804 1105 76 0.832592942423986 1106 1 -0.266424222861108 1106 2 -0.1298725627372157 1106 3 -0.055543449543485224 1106 4 0.3773585308982348 1106 5 0.10632824951444064 1106 6 0.41435508610810956 1106 7 -0.8893431286075 1106 8 -0.35728995548561926 1106 9 -0.058777741615763546 1106 10-11 -0.46979420342043454 1106 12-13 0.12506724556171633 1106 14-15 0.4235081500762803 1106 16-17 0.5403292183859065 1106 18-19 -0.012917688062920263 1106 20-21 0.5258941962926897 1106 22-23 0.23512214871793446 1106 24-25 0.14667679488129437 1106 26-27 0.06023525780137362 1106 28-29 -0.41014168292299047 1106 30-31 -0.25041149565008425 1106 32-33 -0.0034081991991641303 1106 34-35 0.031928311395517994 1106 36-37 0.19904599945620305 1106 38-39 -0.12162190545199536 1106 40-41 0.028678127966713873 1106 42-43 0.09341357219895485 1106 44-45 -0.280383643929099 1106 46-47 -0.3561640887804174 1106 48-49 0.1290318735291578 1106 50-51 0.04831359929025325 1106 52-53 0.39715717931058236 1106 54-55 -0.20971718234532233 1106 56-57 -0.21956891446924232 1106 58-59 -0.29037987634188056 1106 60-61 -0.21880939281635392 1106 62-63 -0.2231228394477327 1106 64-65 0.10490130610006787 1106 66-67 -0.39467425919036003 1106 68-69 0.15903182161522267 1106 70-71 0.20936841208959578 1106 72-73 0.3497166935206977 1106 74-75 -0.10771285523605201 1106 76 -0.06021319269772363 1107 1 0.06980739867075769 1107 2 -0.501200642923191 1107 3 -0.07688813450559451 1107 4 -0.7948028071448192 1107 5 -0.7264465595306362 1107 6 -1.5738555228343145 1107 7 -0.5411862587587564 1107 8 -0.39025798374392906 1107 9 -0.12273632024692205 1107 10-11 -0.6161502098208231 1107 12-13 -0.13144635525947024 1107 14-15 -0.3444627462797243 1107 16-17 0.29676734295190954 1107 18-19 -0.47827408693983386 1107 20-21 0.20885618645059623 1107 22-23 0.46158865235589275 1107 24-25 0.2874475583990943 1107 26-27 -0.042472830234132175 1107 28-29 -0.13308344544449824 1107 30-31 -0.021054251439117877 1107 32-33 -0.15488904332560338 1107 34-35 -0.04187237090982876 1107 36-37 0.3776681049418116 1107 38-39 0.09756113956204615 1107 40-41 0.05372114943215678 1107 42-43 0.40982512912314917 1107 44-45 0.29245094231006874 1107 46-47 0.07226177844175652 1107 48-49 -0.05152242408881591 1107 50-51 0.00966975664314873 1107 52-53 -0.04221274491125371 1107 54-55 0.1178573127185345 1107 56-57 0.1873613152804836 1107 58-59 0.1227243110807521 1107 60-61 0.20585775689833952 1107 62-63 -0.04345161401525388 1107 64-65 0.08440195519585814 1107 66-67 0.16144988415414474 1107 68-69 0.15380131713193634 1107 70-71 -0.09320079963639927 1107 72-73 -0.07269371616441589 1107 74-75 0.24464804202567336 1107 76 0.022871068993545407 1108 1 -0.34311324422226264 1108 2 0.012250512275201686 1108 3 0.2284232668002808 1108 4 0.37841416924895555 1108 5 0.40426664991185746 1108 6 0.2905970135795499 1108 7 0.03143742280829542 1108 8 0.38483932916415853 1108 9 -0.20518546640463597 1108 10-11 0.42173119399635794 1108 12-13 0.4190935744241102 1108 14-15 0.3595333612039511 1108 16-17 0.20909939970731273 1108 18-19 0.3674614102977358 1108 20-21 0.31300454154852986 1108 22-23 0.25978993311932186 1108 24-25 -0.16694093984300196 1108 26-27 0.12946495228721489 1108 28-29 0.25188327981918235 1108 30-31 0.43451926689337483 1108 32-33 0.5564604669707336 1108 34-35 0.433901423665759 1108 36-37 -0.017390910628243716 1108 38-39 0.1218579753229676 1108 40-41 0.2850119580710313 1108 42-43 -0.1284800567131157 1108 44-45 -0.07930006221026531 1108 46-47 0.5999881094312087 1108 48-49 0.34419580795538707 1108 50-51 0.5711495406712785 1108 52-53 0.2027489577507282 1108 54-55 0.3678023780123567 1108 56-57 0.37654074772648727 1108 58-59 0.3222707073640336 1108 60-61 0.12580219436695472 1108 62-63 0.21123353164019676 1108 64-65 0.5680471083852154 1108 66-67 0.47779531358659 1108 68-69 -0.08351661355616358 1108 70-71 0.482507073291778 1108 72-73 0.0920633154779793 1108 74-75 0.22521685166906025 1108 76 0.49168385151489247 1109 1 -0.21854673443776917 1109 2 -0.6929374366968055 1109 3 -0.2968739836332084 1109 4 0.4164064902794209 1109 5 -0.49212918858207644 1109 6 -0.25544584002461335 1109 7 0.023441076511538483 1109 8 0.11269262368979582 1109 9 0.1620349324063639 1109 10-11 0.24034263194532457 1109 12-13 -0.23742735696058048 1109 14-15 0.12855274440929776 1109 16-17 -0.251108520778196 1109 18-19 -0.08889928452515505 1109 20-21 -0.29408689392335674 1109 22-23 0.3832959400551701 1109 24-25 -0.19287294380633568 1109 26-27 0.027660549265164036 1109 28-29 -0.6501247087862154 1109 30-31 -0.06582520301001438 1109 32-33 -0.26918744608351375 1109 34-35 -0.037747542150441404 1109 36-37 -0.16516462873575222 1109 38-39 -0.2846629560617231 1109 40-41 -0.4454809822311745 1109 42-43 -0.20532006786000778 1109 44-45 -0.0033850882196873044 1109 46-47 -0.03367782443057621 1109 48-49 0.01415493602078044 1109 50-51 0.0019846286078717412 1109 52-53 -0.3177257140352552 1109 54-55 -0.17366947608721972 1109 56-57 -0.07979285395793312 1109 58-59 0.09310533084681794 1109 60-61 0.25038031549649986 1109 62-63 0.04898820714180374 1109 64-65 -0.10734612916742492 1109 66-67 0.41914253924920786 1109 68-69 0.035321632666281744 1109 70-71 0.29147335394572593 1109 72-73 -0.005928695916615823 1109 74-75 -0.29848577404310106 1109 76 0.19041589263453318 1110 1 -0.5882431143521316 1110 2 -0.32962636085166963 1110 3 0.05197471535172937 1110 4 -0.13544696961218605 1110 5 -0.13469438904918363 1110 6 -0.034452936470401596 1110 7 -0.018418970798101952 1110 8 -0.8929141673393346 1110 9 -0.3474494524186227 1110 10-11 -0.7616635362733746 1110 12-13 -0.7195178141872773 1110 14-15 -0.3608400154194271 1110 16-17 -0.18380457132165873 1110 18-19 -0.3871745787132568 1110 20-21 -0.9387593195903321 1110 22-23 -0.4070744524950598 1110 24-25 -0.13153884194090182 1110 26-27 -0.8301223354250737 1110 28-29 -0.371274812088906 1110 30-31 -0.16933000883089733 1110 32-33 -0.2834027947675324 1110 34-35 -0.6366374124980752 1110 36-37 -0.11185894259627815 1110 38-39 -0.529116050651055 1110 40-41 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-0.5993229371001263 2116 10-11 -0.4326701800593895 2116 12-13 -0.8058673529007123 2116 14-15 -0.2649538099343687 2116 16-17 -0.6422494216003187 2116 18-19 -0.5772466842581423 2116 20-21 -0.17650688720895857 2116 22-23 -0.061314195560015605 2116 24-25 -0.5182205699550053 2116 26-27 -0.3846541216903816 2116 28-29 -0.5698720601130844 2116 30-31 -0.31383235462264025 2116 32-33 -0.4971138687937895 2116 34-35 -0.14655979670412478 2116 36-37 -0.3072287590262661 2116 38-39 -0.3502376224889616 2116 40-41 -0.557915887722146 2116 42-43 -0.36561456439741136 2116 44-45 -0.2895354750782033 2116 46-47 -0.4865130953096184 2116 48-49 -0.28152052442283804 2116 50-51 -0.22375961535893651 2116 52-53 -0.10096783047537627 2116 54-55 -0.023188928317416924 2116 56-57 8.813963328933028E-4 2116 58-59 -0.6673546833627171 2116 60-61 -0.39008329013422127 2116 62-63 -0.15063235140226539 2116 64-65 -0.5793057178716197 2116 66-67 -0.11789203989088293 2116 68-69 -0.5334384682058086 2116 70-71 -0.11657064877125833 2116 72-73 -0.1363246110653975 2116 74-75 -0.1314027107169764 2116 76 -0.22833601042832186 2117 1 0.14506986896085294 2117 2 0.02303949181418119 2117 3 -0.034105870728854626 2117 4 -0.23790932207453608 2117 5 0.20749120313641 2117 6 0.0845946825772188 2117 7 0.6437706913915662 2117 8 -0.19277171719688369 2117 9 -0.14873150370067378 2117 10-11 0.1475753729655338 2117 12-13 -0.3406513103207729 2117 14-15 -0.4476328522122657 2117 16-17 -0.43583957335665957 2117 18-19 -0.033491225029905536 2117 20-21 -0.4840889899200036 2117 22-23 -0.03751808293868919 2117 24-25 -0.12762146302351596 2117 26-27 -0.04710442740716303 2117 28-29 -0.14345633427043225 2117 30-31 0.2648376753367856 2117 32-33 0.06671930535457449 2117 34-35 7.760999154413639E-4 2117 36-37 -0.04317762391496416 2117 38-39 -0.24828576982077522 2117 40-41 0.18180035485942625 2117 42-43 -0.31106309867116266 2117 44-45 0.36669029003007836 2117 46-47 0.18717811849621313 2117 48-49 0.04611888487846727 2117 50-51 0.32620813947988125 2117 52-53 0.1547934410452072 2117 54-55 0.30041779500276533 2117 56-57 -0.5570823421466002 2117 58-59 0.025614238832567082 2117 60-61 -0.2825966984069481 2117 62-63 -0.12902401174234512 2117 64-65 -0.11326926043115293 2117 66-67 0.05386854590982182 2117 68-69 -0.6314964115359558 2117 70-71 -0.22934299793329416 2117 72-73 -1.6279816858170264 2117 74-75 -0.27522021126799956 2117 76 -0.48959528601424296 2118 1 -0.008945293528888953 2118 2 -0.30575475223430004 2118 3 -0.09560087290182295 2118 4 -1.1846957434372314 2118 5 -0.6433707409458727 2118 6 -0.4025848046022702 2118 7 -0.5896302453992064 2118 8 -0.46690373709162003 2118 9 -0.17589360713395763 2118 10-11 -1.5473157371798152 2118 12-13 -0.3327682643021319 2118 14-15 -0.5256907014565542 2118 16-17 -0.9851176650076354 2118 18-19 -0.6213274315101742 2118 20-21 -1.1049060259912764 2118 22-23 -0.6859438881441591 2118 24-25 -0.569772218733597 2118 26-27 -0.9610669557764737 2118 28-29 -0.9909743216189213 2118 30-31 -0.4210839046525905 2118 32-33 -0.8254652520842214 2118 34-35 -0.22837547669376335 2118 36-37 -0.8532601967093996 2118 38-39 -0.8023202475840634 2118 40-41 -0.9156814163511555 2118 42-43 -0.8017193350901053 2118 44-45 -0.7250210432066737 2118 46-47 -0.7522926430084453 2118 48-49 -0.7031205762254018 2118 50-51 -0.6761700535762856 2118 52-53 -0.7360511192908774 2118 54-55 -0.7796440138725984 2118 56-57 -0.7593253282878933 2118 58-59 -0.5470715499549144 2118 60-61 -0.7535875719401872 2118 62-63 -0.3640184586012012 2118 64-65 -0.3407548946964454 2118 66-67 -0.4950956725459221 2118 68-69 -0.5039069576753192 2118 70-71 -0.7259145368777169 2118 72-73 -0.7413502695245882 2118 74-75 -0.5917130404726976 2118 76 -0.36747446897817326 2119 1 -0.2515657467758885 2119 2 -0.1904116429852678 2119 3 -0.3626135054162667 2119 4 -0.10102914025053522 2119 5 -0.09777625100744913 2119 6 -0.35764098437755365 2119 7 -0.38349490609875403 2119 8 -0.16581822956923986 2119 9 -0.343560083360714 2119 10-11 -0.05744079272479041 2119 12-13 -0.0242159250651639 2119 14-15 -0.2759684263343516 2119 16-17 -0.2810850997819614 2119 18-19 -0.49507370206181633 2119 20-21 -0.6754243399632145 2119 22-23 -0.9581301383822023 2119 24-25 -0.23633573044565281 2119 26-27 -0.5435591794798995 2119 28-29 -0.26059756798469635 2119 30-31 -0.6378600742710745 2119 32-33 -0.6722007945205846 2119 34-35 -0.6992222995211677 2119 36-37 -0.41428825485705545 2119 38-39 -0.7989796140539482 2119 40-41 -0.40344565173489144 2119 42-43 -0.5334053631482547 2119 44-45 -0.40421706936042057 2119 46-47 -0.5444354094656276 2119 48-49 -0.2499053156401203 2119 50-51 -0.46846358496712526 2119 52-53 -0.4522242291747105 2119 54-55 -0.8748719069723236 2119 56-57 -0.26866481917851814 2119 58-59 -0.4838931077534383 2119 60-61 -0.7523100375023049 2119 62-63 -0.6322451455814573 2119 64-65 -0.8685591224625853 2119 66-67 -0.7941339509690764 2119 68-69 -0.6358659496133683 2119 70-71 -0.16233140985428918 2119 72-73 -0.6286019041338733 2119 74-75 -0.7983403495566819 2119 76 -0.3630467409263751 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 31.0 13 292.0 14 1082.0 15 1763.0 16 2192.0 17 2610.0 18 2636.0 19 2257.0 20 1839.0 21 1331.0 22 1005.0 23 794.0 24 872.0 25 849.0 26 955.0 27 1110.0 28 1371.0 29 1672.0 30 2064.0 31 2553.0 32 3327.0 33 4463.0 34 6047.0 35 9385.0 36 18011.0 37 64364.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.360568225629464 17.02749191850292 9.810492600610932 29.80144725525668 2 24.138467926095082 19.207271864527417 28.965123520863607 27.689136688513894 3 20.05397550342537 21.639135205670392 26.731219787063676 31.575669503840565 4 16.864379133426258 17.644354814792848 29.99940686259972 35.49185918918118 5 16.582638868293838 30.257421631720987 32.489842522020226 20.670096977964945 6 31.90782644799668 31.11672944037486 17.697737180817935 19.277706930810524 7 30.456122660814376 27.224265251045406 19.019692161689257 23.29991992645096 8 26.343456211631423 29.92229900056348 20.131083365461606 23.603161422343486 9 25.056348053026483 17.65473471929773 19.64174501023162 37.64717221744417 10-11 27.007399389068475 22.149974791660487 24.521041549274887 26.321584269996144 12-13 26.073949405379754 20.15777454847415 24.146252854473737 29.622023191672355 14-15 24.855793469557224 21.150167561315577 22.021338117975027 31.97270085115217 16-17 26.022791304605715 23.43189299801299 22.813176547347194 27.732139150034108 18-19 25.16088851982562 22.688246982413478 23.775541979299504 28.3753225184614 20-21 24.81242029716184 22.93810611228091 24.13068299771642 28.11879059284083 22-23 26.368664551143272 22.73792223968682 23.287315756695037 27.606097452474863 24-25 25.404074853939917 23.629111183605684 22.9685044040452 27.998309558409208 26-27 24.510290933894836 24.017986891663455 24.06284290755954 27.408879266882174 28-29 25.21352946410036 22.91289777276906 25.33734689640855 26.536225866722024 30-31 25.088229188291468 22.494735905572526 24.86172484356001 27.555310062575995 32-33 24.06580859456093 23.726608143776506 24.980352323615765 27.227230938046798 34-35 24.46654705062428 23.438565793766124 24.867656217562796 27.227230938046798 36-37 24.863578397935882 22.160354696165367 25.21760728372728 27.75845962217148 38-39 23.62206767697737 22.507710786203624 26.92806726178119 26.94215427503781 40-41 23.66840653637415 21.966843619324415 27.254292831934517 27.11045701236692 42-43 22.547747560722442 21.818188558379546 27.138631038880156 28.495432842017856 44-45 21.52347341261603 21.78074675998695 28.660769892345563 28.035009935051452 46-47 21.459711142086064 22.545152584596224 28.80608855541386 27.189047717903854 48-49 21.332928022776475 22.861368961119844 28.51248554227587 27.293217473827813 50-51 20.780198107891692 23.90010083335805 27.260965627687657 28.058735431062605 52-53 20.101797206322843 23.713633263145407 27.722500667279576 28.46206886325217 54-55 20.002817402651324 23.281013671817078 27.876345680476884 28.839823245054717 56-57 19.98576470239331 23.78925828168095 27.47820220053975 28.746774815385983 58-59 20.83951184791957 23.546813369317 26.694519410421425 28.919155372342004 60-61 20.452860405112844 22.951822414662356 27.083765829354373 29.51155135087043 62-63 20.499569975384798 22.859886117619148 26.854666508496695 29.78587739849936 64-65 21.142753343812092 22.927355496900855 26.188499065808596 29.741392093478453 66-67 21.03228150301017 23.88972092885317 24.676740116848066 30.401257451288593 68-69 20.94738871259527 24.771271390017496 24.598520122186304 29.682819775200926 70-71 21.01522880275216 25.24133277973843 25.124188143183368 28.619250274326046 72-73 21.132373439307216 24.70862125211305 26.021308461105015 28.137696847474718 74-75 20.946647290844925 25.91676799430588 25.477475607224413 27.65910910762478 76 20.49512144488271 25.96755538420475 24.590735193807646 28.946587977104898 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 1.0 16 6.0 17 10.0 18 8.5 19 10.5 20 21.0 21 28.0 22 38.5 23 67.5 24 81.5 25 77.0 26 144.0 27 294.5 28 402.0 29 426.0 30 551.0 31 770.0 32 1085.5 33 1307.0 34 1520.0 35 2031.5 36 2601.5 37 2873.0 38 3255.5 39 4249.0 40 5217.0 41 6016.0 42 6458.0 43 6511.5 44 6990.0 45 7376.5 46 7338.0 47 7279.0 48 7134.0 49 6602.0 50 6156.0 51 5786.0 52 4906.5 53 4099.0 54 3801.0 55 3736.0 56 3441.0 57 3263.0 58 3315.0 59 3275.0 60 3264.0 61 3215.5 62 3138.0 63 3258.5 64 3364.5 65 3444.5 66 3489.5 67 3440.0 68 3361.5 69 3066.5 70 2686.5 71 2523.0 72 2296.5 73 1857.0 74 1317.0 75 990.0 76 765.5 77 385.5 78 148.0 79 66.0 80 50.0 81 25.0 82 13.0 83 10.0 84 11.0 85 11.5 86 8.5 87 6.0 88 5.0 89 5.0 90 4.5 91 5.5 92 8.0 93 4.5 94 3.0 95 5.0 96 5.0 97 3.5 98 3.0 99 13.0 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 134876.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.2193718674931 #Duplication Level Percentage of deduplicated Percentage of total 1 75.36910592356915 46.89418428779027 2 13.632192947961725 16.963729647972954 3 4.6961951405521996 8.76582935436994 4 2.0615116958019044 5.1306385124114 5 1.1546848747006042 3.5921883804383286 6 0.7566820386324908 2.824816868827664 7 0.5398062417330998 2.351048370354993 8 0.37893683194508987 1.8861769328865032 9 0.30028956493761844 1.681544529790326 >10 1.1094031149084236 9.86980634063881 >50 0.0011916252576889622 0.040036774518817285 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.010379904504878554 0.0 2 0.0 0.0 0.0 0.01705270025801477 0.0 3 0.0 0.0 0.0 0.01705270025801477 0.0 4 0.0 0.0 0.0 0.08155639253833151 0.0 5 0.0 0.0 0.0 0.08600492304042232 0.0 6 0.0 0.0 0.0 0.08600492304042232 0.0 7 0.0 0.0 0.0 0.08897061004181618 0.0 8 0.0 0.0 0.0 0.08971203179216465 0.0 9 0.0 0.0 0.0 0.09712624929564934 0.0 10 0.0 0.0 0.0 0.10231620154808861 0.0 11 0.0 0.0 0.0 0.10454046679913402 0.0 12 0.0 0.0 0.0 0.10602331029983096 0.0 13 0.0 0.0 0.0 0.10602331029983096 0.0 14 0.0 0.0 0.0 0.10750615380052789 0.0 15 0.0 0.0 0.0 0.11640321480470951 0.0 16 0.0 0.0 0.0 0.11936890180610338 0.0 17 0.0 0.0 0.0 0.12233458880749726 0.0 18 0.0 0.0 0.0 0.12678311930958808 0.0 19 0.0 0.0 0.0 0.1327144933123758 0.0 20 0.0 0.0 0.0 0.13345591506272428 0.0 21 0.0 0.0 0.0 0.13864586731516357 0.0 22 0.0 0.0 0.0 0.14902577182004212 0.0 23 0.0 0.0 0.0 0.151991458821436 0.0 24 0.0 0.0 0.0 0.1586642545745722 0.0 25 0.0 0.0 0.0 0.1727512678311931 0.0 26 0.0 0.0 0.0 0.1846140158367686 0.0 27 0.0 0.0 0.0 0.2713603606275394 0.0 28 0.0 0.0 0.0 0.4025920104392182 0.0 29 0.0 0.0 0.0 0.5493935170082149 0.0 30 0.0 0.0 0.0 0.7258518935911504 0.0 31 0.0 0.0 0.0 0.897861739671995 0.0 32 0.0 0.0 0.0 1.084700020759809 0.0 33 0.0 0.0 0.0 1.3204721373706219 0.0 34 0.0 0.0 0.0 1.6096266200065246 0.0 35 0.0 0.0 0.0 2.010735786945046 0.0 36 0.0 0.0 0.0 2.649099913995077 0.0 37 0.0 0.0 0.0 3.464663839378392 0.0 38 0.0 0.0 0.0 4.375129748806311 0.0 39 0.0 0.0 0.0 5.353806459266289 0.0 40 0.0 0.0 0.0 6.404401079510069 0.0 41 0.0 0.0 0.0 7.47353124351256 0.0 42 0.0 0.0 0.0 8.492986150241704 0.0 43 0.0 0.0 0.0 9.552477831489664 0.0 44 0.0 0.0 0.0 10.625315104243898 0.0 45 0.0 0.0 0.0 11.672202615735936 0.0 46 0.0 0.0 0.0 12.757644058246093 0.0 47 0.0 0.0 0.0 13.874225214270886 0.0 48 0.0 0.0 0.0 14.881075951244105 0.0 49 0.0 0.0 0.0 15.82268157418666 0.0 50 0.0 0.0 0.0 16.72795753136214 0.0 51 0.0 0.0 0.0 17.67252884130609 0.0 52 0.0 0.0 0.0 18.652688395266765 0.0 53 0.0 0.0 0.0 19.543135917435272 0.0 54 0.0 0.0 0.0 20.415789317595422 0.0 55 0.0 0.0 0.0 21.292891248257657 0.0 56 0.0 0.0 0.0 22.155164743912927 0.0 57 0.0 0.0 0.0 23.051543640084226 0.0 58 0.0 0.0 0.0 23.87674604822207 0.0 59 0.0 0.0 0.0 24.684895756101902 0.0 60 0.0 0.0 0.0 25.500459681485218 7.414217503484682E-4 61 0.0 0.0 0.0 26.275986832349712 7.414217503484682E-4 62 0.0 0.0 0.0 27.049289717963166 7.414217503484682E-4 63 0.0 0.0 0.0 27.85521516059195 7.414217503484682E-4 64 0.0 0.0 0.0 28.651502120466205 7.414217503484682E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACTGGA 15 0.0022206362 70.00001 2 ACGCTTC 25 2.3834499E-4 56.000004 15 CCGACAA 20 0.0069388044 52.5 21 GACGCTT 20 0.0069388044 52.5 14 TCGCGAA 35 0.001251832 40.0 11 CTTCCGA 40 0.002411894 35.0 18 CCTTGCG 40 0.002411894 35.0 54 TTCTAAG 45 0.0042951177 31.111113 2 AGGAGAT 45 0.0042951177 31.111113 30 TTGCTAG 45 0.0042951177 31.111113 28 TGGAAGG 45 0.0042951177 31.111113 5 ACACTTT 45 0.0042951177 31.111113 39 GGACTCG 50 0.007188173 28.000002 1 CTTGCGG 50 0.007188173 28.000002 55 ATTGCTA 50 0.007188173 28.000002 27 CTGGAAG 50 0.007188173 28.000002 4 TAGATCT 75 5.5521064E-5 28.0 39 AGATCTC 100 3.908852E-4 21.000002 40 GGTCTAG 105 5.4322736E-4 20.000002 1 ATCCACA 100 0.009647397 17.500002 4 >>END_MODULE