FastQCFastQC Report
Wed 25 May 2016
SRR1780274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780274_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134876
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT75865.62442539814348No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC22211.646697707523948No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG20541.5228802752157538No Hit
CACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTG17631.3071265458643495RNA PCR Primer, Index 32 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC4720.349951066164477No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT2360.1749755330822385RNA PCR Primer, Index 42 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT2200.16311278507666302No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT1570.11640321480470951No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC1450.1075061538005279No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG1380.10231620154808861No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAA150.002220636270.0000133
TCTTTAT550.063.636361
TTAATAC252.3834499E-456.0000043
ATCGTAT252.3834499E-456.00000439
TCGTTCC200.006938804452.556
GTTTAGC200.006938804452.51
AGCGGTC200.006938804452.512
GTTAAGT200.006938804452.521
AACATGC200.006938804452.512
GAGCGGT200.006938804452.511
CCTTTAA200.006938804452.51
GGAGTGA200.006938804452.562
TGCTAGC200.006938804452.516
CTTTATA707.2759576E-1250.02
CAATCTT305.860858E-446.6666684
CTTAATA404.5731587E-543.752
TCGATTC350.00125183240.047
GTTCGAT350.00125183240.045
TTCGATT350.00125183240.046
GTACGAT350.00125183240.020
Produced by FastQC (version 0.11.5)