FastQCFastQC Report
Wed 25 May 2016
SRR1780273_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780273_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683601
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACAC501.3624231E-939.551753
CGAATTT353.191152E-431.47083918
TGATACA602.971883E-729.2997342
AGTCTCT450.001393219524.45759212
GGGCTAA450.001406954824.4164451
TACACAG450.001406954824.4164455
ATTCAAT500.002583314221.99410628
AAGATAC500.002583314221.99410626
GGGACTA500.00259652121.9748021
CACTAGC500.00259652121.9748021
GATCCTA904.1216845E-721.97481
GGTATAG1306.91216E-1121.97481
TAATACG500.002597624221.9731944
GGACAAT654.938054E-420.30225230
AATCGTC654.938054E-420.30225228
CTGATAC654.967385E-420.2844311
CAATTCA550.004488603719.99464226
CCCTAGT550.00451147519.977091
CTACAGC550.004513385219.975634
GAAACTC708.13302E-418.8520926
Produced by FastQC (version 0.11.5)