FastQCFastQC Report
Wed 25 May 2016
SRR1780273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780273_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683601
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT208283.046806543583172No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC66020.9657680430543549No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG57730.8444984720619192No Hit
CACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTG40060.5860143563277409Illumina PCR Primer Index 4 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC11870.17363930128832464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCA357.2912826E-637.7102118
TCTTCTG5450.034.31072644
TCTTTAT1650.033.4299851
CCGTCTT5950.032.5366841
ATGCCGT30150.031.95908444
CTTTATA1800.030.563432
CGTCTTC6200.030.51506442
GTCTATG300.00573605929.3408972
GGGCTTA300.005744268729.332316
CATCGTT300.005746322729.33016632
ATTTGCG300.005746322729.33016631
GTCTTCT6500.029.10667443
TATGCCG34900.027.60935443
CAAGGAA609.725416E-625.6732854
GCCGTCT7700.024.57057240
TTTATAC2600.024.5447863
TTGGCAA450.001398473324.44180328
TCCGATT551.5937982E-423.99740816
CCGATTC752.0640236E-623.4641317
AGACTTG44450.022.12134625
Produced by FastQC (version 0.11.5)