FastQCFastQC Report
Wed 25 May 2016
SRR1780272_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780272_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92859
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA207.890355E-570.0000153
CCGGGTA150.002218451670.047
GATTACG150.002218451670.019
GTAAGGT252.379569E-455.99999619
CGGACAA200.00693202752.5000045
GGATGTG200.00693202752.50000423
CGACGAT407.4093987E-752.50000422
GCTAGGT200.00693202752.50000413
ACGAAAG200.00693202752.50000445
GACGATG404.5620924E-543.75000423
TGGTAGT404.5620924E-543.75000469
CTATCGG350.001249817740.038
GCTATCG350.001249817740.037
GCAGGAT350.001249817740.03
GACGCTT350.001249817740.014
GGTAGTA459.136709E-538.8888970
CTTCCGA400.00240803635.00000418
TCAGCTA400.00240803635.00000425
TAAGGTC501.698427E-435.020
ATCGGGC501.698427E-435.040
Produced by FastQC (version 0.11.5)