FastQCFastQC Report
Wed 25 May 2016
SRR1780272_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780272_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92859
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT79378.547367514188178No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC23002.476873539452288No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG19942.1473416685512445No Hit
CACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCTG18992.045036022356476Illumina PCR Primer Index 8 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC5620.6052186648574721No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT2530.27245608933975163RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT1560.16799664006719867No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1390.14968931390602958No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1110.11953607081704519No Hit
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1040.11199776004479911No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCAT150.002218451670.028
AAAGTAC150.002218451670.070
ACTCGCT150.002218451670.07
ACCCATG150.002218451670.029
CTTTATA653.6379788E-1253.8461532
AGCGGTC200.00693202752.50000412
AGCGGCT200.00693202752.50000469
AGTGCCT200.00693202752.5000045
TGCGCGG200.00693202752.50000410
ATATATG200.00693202752.5000042
GAGCGGT200.00693202752.50000411
TGCTGCC200.00693202752.50000446
TTGAATT200.00693202752.50000413
CAGCTTA200.00693202752.5000041
GCTACCA200.00693202752.50000436
GTGAATG200.00693202752.50000429
TCTTTAT707.2759576E-1250.01
AGTGCTT350.001249817740.08
CGTAGCG350.001249817740.015
TTTATAC901.0186341E-1038.888893
Produced by FastQC (version 0.11.5)