FastQCFastQC Report
Wed 25 May 2016
SRR1780271_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780271_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394458
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA353.219453E-431.4194210
CGGCTTA300.005737866329.33595825
CTTAGAC300.005766396429.3062041
GTTATAC300.005766396429.3062041
TTATAGA300.005769970429.302494
TATAGAT300.005769970429.302495
ATCGGTT407.019995E-427.50245925
ACCGTAT508.3439474E-526.39231311
TATCGGC450.001386551724.47458515
CAACCGT450.001405131424.4187419
TCGGTTT651.8081191E-523.69442426
CTACACA752.0773205E-623.441994
GTATCGG500.00256656422.01593614
TATCGGT500.00256656422.01593624
ACGAAGT500.00256656422.01593624
CCGTATC500.00257604322.00196612
TCCTAAC602.8860298E-421.9796541
ACTACAC654.959123E-420.2863393
TGCTAGT654.959123E-420.2863396
CGATATC550.004443243620.02720321
Produced by FastQC (version 0.11.5)