FastQCFastQC Report
Wed 25 May 2016
SRR1780271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780271_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394458
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT172844.381708572268784No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC51781.3126872822962141No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG45141.1443550390662631No Hit
CACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCTG37560.9521926288730359Illumina PCR Primer Index 1 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC10230.2593432000365058No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT4830.12244649620491915Illumina PCR Primer Index 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAT254.4326778E-544.0081143
GGTCCAA302.5246754E-643.99695611
TCTTCTG4700.039.3164344
GTCTTCT4950.037.33075343
TAGATTG301.300075E-436.664135
TCAGTTA301.300075E-436.6641333
TCTTTAT1050.035.6980361
CGTCTTC5200.035.53600342
CCGTCTT5250.035.19756741
ATGCCGT23800.032.1658444
GCCGTCT5850.031.58755940
CAGTTAT353.215266E-431.426434
AGTTATA353.215266E-431.426435
TGATAGA502.3527427E-630.805682
TGTAGGG300.005742323629.3313035
ATAGATT407.0161093E-427.5050724
TGCCGTC7100.026.33620639
TATGCCG30150.025.53720743
AGATTGG450.001397109124.4427556
TTAATAC551.588976E-424.0044272