Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780271_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 394458 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 58 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT | 17284 | 4.381708572268784 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC | 5178 | 1.3126872822962141 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG | 4514 | 1.1443550390662631 | No Hit |
CACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCTG | 3756 | 0.9521926288730359 | Illumina PCR Primer Index 1 (96% over 25bp) |
TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC | 1023 | 0.2593432000365058 | No Hit |
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT | 483 | 0.12244649620491915 | Illumina PCR Primer Index 1 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATAGAT | 25 | 4.4326778E-5 | 44.008114 | 3 |
GGTCCAA | 30 | 2.5246754E-6 | 43.996956 | 11 |
TCTTCTG | 470 | 0.0 | 39.31643 | 44 |
GTCTTCT | 495 | 0.0 | 37.330753 | 43 |
TAGATTG | 30 | 1.300075E-4 | 36.66413 | 5 |
TCAGTTA | 30 | 1.300075E-4 | 36.66413 | 33 |
TCTTTAT | 105 | 0.0 | 35.698036 | 1 |
CGTCTTC | 520 | 0.0 | 35.536003 | 42 |
CCGTCTT | 525 | 0.0 | 35.197567 | 41 |
ATGCCGT | 2380 | 0.0 | 32.16584 | 44 |
GCCGTCT | 585 | 0.0 | 31.587559 | 40 |
CAGTTAT | 35 | 3.215266E-4 | 31.4264 | 34 |
AGTTATA | 35 | 3.215266E-4 | 31.4264 | 35 |
TGATAGA | 50 | 2.3527427E-6 | 30.80568 | 2 |
TGTAGGG | 30 | 0.0057423236 | 29.331303 | 5 |
ATAGATT | 40 | 7.0161093E-4 | 27.505072 | 4 |
TGCCGTC | 710 | 0.0 | 26.336206 | 39 |
TATGCCG | 3015 | 0.0 | 25.537207 | 43 |
AGATTGG | 45 | 0.0013971091 | 24.442755 | 6 |
TTAATAC | 55 | 1.588976E-4 | 24.004427 | 2 |