FastQCFastQC Report
Wed 25 May 2016
SRR1780270_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780270_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66305
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC150.00221564470.020
CACCTTA150.00221564470.053
TCTATCA207.87373E-570.010
GCGAGAC200.00692332352.560
GGTACTG200.00692332352.55
TACCCGA200.00692332352.514
ACCCGAT200.00692332352.515
CTTTATC200.00692332352.540
TAGTAGC200.00692332352.564
CCCGAAG200.00692332352.512
CTTATAA451.6616996E-646.6666641
TACTCTA305.8391894E-446.6666647
GGTCTTT350.001247231240.035
CTTAACA552.9633474E-431.818181
TTATAAA552.9633474E-431.818182
TATTTTC450.004279522731.11110925
AGTGCTT450.004279522731.11110942
CTTATAT450.004279522731.1111091
TGTACCC450.004279522731.11110912
TATCCAC450.004279522731.1111093
Produced by FastQC (version 0.11.5)