##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780270_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 66305 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.019365055425684 34.0 27.0 34.0 12.0 34.0 2 29.132282633285573 34.0 27.0 34.0 12.0 34.0 3 28.829273810421537 34.0 23.0 34.0 11.0 34.0 4 28.72683809667446 34.0 23.0 34.0 11.0 34.0 5 28.66079481185431 34.0 23.0 34.0 11.0 34.0 6 31.376532689842396 37.0 27.0 38.0 11.0 38.0 7 31.741135660960712 37.0 27.0 38.0 12.0 38.0 8 31.734906869768494 37.0 27.0 38.0 11.0 38.0 9 31.9554633888847 37.0 31.0 38.0 12.0 38.0 10-11 31.593100067868185 37.0 27.0 38.0 11.0 38.0 12-13 31.685996531181658 37.0 27.0 38.0 11.0 38.0 14-15 31.482135585551617 37.0 27.0 38.0 11.0 38.0 16-17 31.541693688258803 37.0 27.0 38.0 11.0 38.0 18-19 31.599984918181132 37.0 27.0 38.0 11.0 38.0 20-21 31.60617600482618 37.0 27.5 38.0 11.0 38.0 22-23 31.563230525601387 37.0 27.0 38.0 11.0 38.0 24-25 31.576057612548073 37.0 27.0 38.0 11.0 38.0 26-27 31.50690747304125 37.0 27.0 38.0 11.0 38.0 28-29 31.520714878214314 37.0 27.0 38.0 11.0 38.0 30-31 31.570696025940727 37.0 27.0 38.0 11.0 38.0 32-33 31.61396576427117 38.0 27.0 38.0 11.0 38.0 34-35 31.57188748963125 37.0 27.0 38.0 11.0 38.0 36-37 31.512246436920293 37.0 27.0 38.0 11.0 38.0 38-39 31.48158509916296 37.0 27.0 38.0 11.0 38.0 40-41 31.582331649196895 37.0 27.0 38.0 11.0 38.0 42-43 31.63321770605535 37.0 27.0 38.0 11.0 38.0 44-45 31.750969006862228 38.0 27.0 38.0 11.0 38.0 46-47 31.64072091094186 37.5 27.0 38.0 11.0 38.0 48-49 31.5423874519267 37.0 27.0 38.0 11.0 38.0 50-51 31.500701304577333 37.0 27.0 38.0 11.0 38.0 52-53 31.57016062137094 37.0 27.0 38.0 11.0 38.0 54-55 31.548993288590605 37.0 27.0 38.0 11.0 38.0 56-57 31.508370409471382 37.0 27.0 38.0 11.0 38.0 58-59 31.55503355704698 37.0 27.0 38.0 11.0 38.0 60-61 31.59091320413242 37.0 27.0 38.0 11.0 38.0 62-63 31.408883191312874 37.0 27.0 38.0 11.0 38.0 64-65 31.335057687957168 37.0 26.0 38.0 11.0 38.0 66-67 31.293703340622876 37.0 26.0 38.0 11.0 38.0 68-69 31.229432169519644 37.0 26.0 38.0 11.0 38.0 70-71 31.18656964029862 37.0 26.0 38.0 11.0 38.0 72-73 31.035321619787346 37.0 25.5 38.0 11.0 38.0 74-75 31.050531634115075 37.0 25.0 38.0 11.0 38.0 76 29.7365206243873 36.0 24.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3511148612958124 1101 2 -0.4657882883536004 1101 3 0.08789074111184192 1101 4 -0.3712656935967864 1101 5 -0.2460417319198207 1101 6 -1.163223655451631 1101 7 -1.3740510261681393 1101 8 -0.4533505859792868 1101 9 -0.5271112717280317 1101 10-11 -0.7045365903664944 1101 12-13 -1.830339613548709 1101 14-15 -0.7575603939350479 1101 16-17 -0.6740410794438532 1101 18-19 -1.206628286572748 1101 20-21 -1.2443935254209926 1101 22-23 -0.8008298137083791 1101 24-25 -1.2640118993156086 1101 26-27 -0.959939630450446 1101 28-29 -1.6355556289745756 1101 30-31 -1.392731057627607 1101 32-33 -1.4772498588887046 1101 34-35 -1.4291526505666532 1101 36-37 -1.354178222878545 1101 38-39 -1.010424856175792 1101 40-41 -0.44631325114187703 1101 42-43 -1.6697887986482307 1101 44-45 -1.3765267376394625 1101 46-47 -0.4706040798901654 1101 48-49 -1.3461606094977654 1101 50-51 -1.396940358733989 1101 52-53 -0.9404312763309832 1101 54-55 -0.7563767690276073 1101 56-57 -0.6642830268199411 1101 58-59 -0.40683518110591876 1101 60-61 -1.323085215248355 1101 62-63 -1.20996307546244 1101 64-65 -1.0677619813066386 1101 66-67 -0.7718675509127166 1101 68-69 -1.1524992200956348 1101 70-71 -1.2860581688196326 1101 72-73 -0.7128264301954523 1101 74-75 -0.9994139985292492 1101 76 -1.2101921820953478 1102 1 0.5253108586927837 1102 2 0.2085772662514742 1102 3 0.7134966721069951 1102 4 0.001239296423175773 1102 5 -0.31479707836977866 1102 6 -0.5756844481519465 1102 7 -0.3833538490937194 1102 8 -0.30122626319628054 1102 9 0.06427737954086155 1102 10-11 0.18785719349435226 1102 12-13 0.574422291213196 1102 14-15 -0.024187853302027662 1102 16-17 -0.5134337227287098 1102 18-19 0.08735517507538404 1102 20-21 0.07405885553138702 1102 22-23 -0.7493256791204104 1102 24-25 -0.21288201622464342 1102 26-27 -0.4197521482719466 1102 28-29 -0.4661088083301479 1102 30-31 0.045927339863126804 1102 32-33 0.4226824194118386 1102 34-35 -0.056184301550398885 1102 36-37 -0.3199965861520013 1102 38-39 -0.0916196095262336 1102 40-41 -0.5584283067449576 1102 42-43 -0.29229378866818934 1102 44-45 0.013443322240778599 1102 46-47 -0.45761220649410106 1102 48-49 -0.11588900983993611 1102 50-51 0.07780657596803664 1102 52-53 0.6563216463949928 1102 54-55 -0.11865221812940163 1102 56-57 -0.3104371471507079 1102 58-59 -0.6170575934241427 1102 60-61 -0.3287702737028759 1102 62-63 -0.1734646437544285 1102 64-65 -0.38330945778226067 1102 66-67 -0.1596633878103475 1102 68-69 0.1523018919659549 1102 70-71 0.4607874257056004 1102 72-73 -0.3143020503836489 1102 74-75 -0.8968156238500313 1102 76 -0.4758751019698835 1103 1 0.9793151876971109 1103 2 0.19694306711727805 1103 3 0.3771871482974696 1103 4 0.7834362661201446 1103 5 0.45874188266918026 1103 6 0.2592722618047638 1103 7 0.36989290415303344 1103 8 0.09217200520198787 1103 9 0.23527305053652725 1103 10-11 -0.19715362901646927 1103 12-13 0.4225012955421974 1103 14-15 0.23027643241225704 1103 16-17 0.29379030324531286 1103 18-19 0.27918417940438545 1103 20-21 0.6220837473062808 1103 22-23 0.25811479707006413 1103 24-25 0.8002402781476441 1103 26-27 0.6324664664466724 1103 28-29 0.9479875120161658 1103 30-31 0.6001751753609597 1103 32-33 1.0664865319659533 1103 34-35 0.14430271143661244 1103 36-37 0.7434503835449746 1103 38-39 0.3772656718590497 1103 40-41 0.019493771177121033 1103 42-43 -0.0659680310924351 1103 44-45 0.2918524131498721 1103 46-47 0.3143033779214903 1103 48-49 0.03833176938084293 1103 50-51 0.08781739847885817 1103 52-53 0.612355196178541 1103 54-55 0.10293869096150843 1103 56-57 -0.0874934242069827 1103 58-59 -0.016814086930633465 1103 60-61 0.4490976916650986 1103 62-63 0.23143254239275635 1103 64-65 0.15037560715280662 1103 66-67 0.8795682139212495 1103 68-69 0.8268148789789436 1103 70-71 0.022069893238064964 1103 72-73 0.6369966509150551 1103 74-75 0.3990447657603582 1103 76 1.0812136426188594 1104 1 -0.10597592568388947 1104 2 1.0242540987822402 1104 3 1.0885635527918502 1104 4 1.5578872364980825 1104 5 0.35474545774578914 1104 6 1.0321272158394947 1104 7 0.7540859582899131 1104 8 1.0024120460600052 1104 9 0.8149461863894416 1104 10-11 0.8468325814023245 1104 12-13 0.9955030830805001 1104 14-15 1.1035208144347344 1104 16-17 0.8697926015088449 1104 18-19 0.9550651804258372 1104 20-21 0.6235840026688955 1104 22-23 0.7338872689801761 1104 24-25 1.353898347606279 1104 26-27 1.224141645200504 1104 28-29 0.30061136288440693 1104 30-31 1.3552505073323573 1104 32-33 0.48946278324276093 1104 34-35 0.8166086358492741 1104 36-37 1.0709210168442524 1104 38-39 1.508968940500523 1104 40-41 1.4711663963048025 1104 42-43 1.210047035301848 1104 44-45 1.593499502016062 1104 46-47 1.1193340214352716 1104 48-49 0.5613782453767584 1104 50-51 1.1863873678353407 1104 52-53 0.8842844607342073 1104 54-55 0.9178646358031806 1104 56-57 1.1273303755887198 1104 58-59 1.3375973022419885 1104 60-61 0.6712631666395588 1104 62-63 0.9779532267645621 1104 64-65 1.42026197078917 1104 66-67 0.7638251087118597 1104 68-69 0.9810538983865058 1104 70-71 1.358159014790676 1104 72-73 1.3881457826821624 1104 74-75 1.4590230599380902 1104 76 1.9584480654993506 1105 1 -0.12308815608554369 1105 2 -0.586168218149183 1105 3 -0.5344507827370144 1105 4 0.031411710216278976 1105 5 -0.9348709699953943 1105 6 -1.134797748644555 1105 7 -0.8679597604237657 1105 8 -1.2183034388941465 1105 9 -0.8979557895888632 1105 10-11 -0.4318061891000653 1105 12-13 -0.8734430618245739 1105 14-15 -0.33545632620843335 1105 16-17 -0.4534994041737015 1105 18-19 0.5274208565203757 1105 20-21 -0.2673820311681183 1105 22-23 0.1104854867252385 1105 24-25 0.08155558640261162 1105 26-27 -0.513040325611847 1105 28-29 -0.03630585266512654 1105 30-31 -0.3504191297592065 1105 32-33 -0.8949489434781448 1105 34-35 -0.2963320848016302 1105 36-37 -1.3903988850025755 1105 38-39 -0.7603388213488955 1105 40-41 -0.34773454648222923 1105 42-43 -0.7822363174038252 1105 44-45 -0.30408541339140527 1105 46-47 0.1401915701889891 1105 48-49 -0.8329456600862422 1105 50-51 -0.8664668230467392 1105 52-53 -0.6255954307807059 1105 54-55 -0.7537096893937694 1105 56-57 -1.1520832883658159 1105 58-59 -0.4540855080382613 1105 60-61 -0.30116354135476087 1105 62-63 -0.3167322956427725 1105 64-65 -0.7205426269119783 1105 66-67 -0.5049014830484424 1105 68-69 -0.4660273362770653 1105 70-71 -0.8073775989249405 1105 72-73 -0.6802708516972764 1105 74-75 -0.8663353282835331 1105 76 -0.051819272741642664 1106 1 -1.7217633947374011 1106 2 -0.9350869791076839 1106 3 -1.1944936426629837 1106 4 -1.383647709226544 1106 5 -1.9551320259081173 1106 6 -1.9820605579178867 1106 7 -2.2420100234278593 1106 8 -3.410588652219687 1106 9 -2.7783376325656803 1106 10-11 -2.42362383959685 1106 12-13 -2.2625430033792178 1106 14-15 -1.8221600082657083 1106 16-17 -2.4518921569498566 1106 18-19 -1.5349208539803065 1106 20-21 -2.3263144293757954 1106 22-23 -2.8599996097095968 1106 24-25 -2.357807359243207 1106 26-27 -2.71126748321948 1106 28-29 -2.431201625117879 1106 30-31 -2.5823010701449434 1106 32-33 -3.0824124151321506 1106 34-35 -2.4004135186609723 1106 36-37 -2.3602508234401327 1106 38-39 -2.2500134755472203 1106 40-41 -1.6827725359620658 1106 42-43 -2.3297231671992655 1106 44-45 -2.282460632561481 1106 46-47 -2.4348620047184752 1106 48-49 -2.5108143528584996 1106 50-51 -2.619681317494436 1106 52-53 -2.214188845937535 1106 54-55 -2.734471427168952 1106 56-57 -2.543033999450948 1106 58-59 -2.0829575127138895 1106 60-61 -2.323372775720628 1106 62-63 -2.0047802208327177 1106 64-65 -2.4074216450300376 1106 66-67 -2.557161370054093 1106 68-69 -3.245347421996918 1106 70-71 -2.496789249032094 1106 72-73 -2.2748549155975297 1106 74-75 -2.435203436602251 1106 76 -3.048312551525978 1107 1 0.27697752535944886 1107 2 0.2333391710133803 1107 3 -0.20459856598824544 1107 4 -0.5447130845292065 1107 5 -0.07693993551263745 1107 6 -0.5366368291043244 1107 7 -0.23406813480800182 1107 8 -0.23932150129151708 1107 9 0.5918964271599094 1107 10-11 0.33190481254197124 1107 12-13 0.3668032435941484 1107 14-15 -0.31954499615916987 1107 16-17 -0.08057657987157185 1107 18-19 0.576878984599194 1107 20-21 0.789892188864723 1107 22-23 0.9432933684986295 1107 24-25 0.4258084599658254 1107 26-27 0.4287002326804341 1107 28-29 0.4715102392889001 1107 30-31 -0.40383456489878 1107 32-33 0.5142300384594556 1107 34-35 0.22667284130674403 1107 36-37 0.5511938900384763 1107 38-39 -0.239714847621471 1107 40-41 -0.5902140210306683 1107 42-43 0.25794430656990386 1107 44-45 -0.2644138206163653 1107 46-47 0.2577449363630393 1107 48-49 -0.3005318669827943 1107 50-51 0.011973242634702785 1107 52-53 0.21405974163308983 1107 54-55 0.06706206758488165 1107 56-57 -0.15817524238880054 1107 58-59 -0.5613433077098549 1107 60-61 0.0989678215352221 1107 62-63 0.7389163086265214 1107 64-65 0.49454768507488467 1107 66-67 0.0470032788563195 1107 68-69 -0.034483822319756996 1107 70-71 0.07543028284845477 1107 72-73 0.15069794961635452 1107 74-75 -0.26288705242146193 1107 76 0.23507727898249442 1108 1 -1.0227050143230905 1108 2 -0.08348622581201681 1108 3 -0.432535073924754 1108 4 -0.24042737024349137 1108 5 0.04750450893180869 1108 6 -0.0344146068821054 1108 7 0.224185833445965 1108 8 -0.15201991398993187 1108 9 -0.8627067474432693 1108 10-11 -0.8546031239659655 1108 12-13 -0.5525618357709305 1108 14-15 -0.20375134536552153 1108 16-17 -0.6170051513001376 1108 18-19 -0.04240672968652248 1108 20-21 -0.9805046365321033 1108 22-23 -0.20646853626326944 1108 24-25 -1.165858206700836 1108 26-27 -1.1667759577957533 1108 28-29 -0.6129342051555433 1108 30-31 -0.7453425014067179 1108 32-33 -0.5386271043976869 1108 34-35 -0.6426922380583378 1108 36-37 -0.8533299194853328 1108 38-39 -1.2844767523833767 1108 40-41 -0.551087036903688 1108 42-43 -0.6228493442237486 1108 44-45 -0.6615566777592221 1108 46-47 -0.8185249049067984 1108 48-49 -0.8466429780939038 1108 50-51 -0.8061219954605328 1108 52-53 -0.6053847028113566 1108 54-55 -0.8464299959071795 1108 56-57 -0.8737307979443578 1108 58-59 -0.6769782283447725 1108 60-61 -0.7198417022743016 1108 62-63 -0.5754487707385536 1108 64-65 -0.7464046958774944 1108 66-67 -0.9104570386039974 1108 68-69 -0.9292457270816641 1108 70-71 -0.7909982885801163 1108 72-73 -1.0801750662566612 1108 74-75 0.07600183646742664 1108 76 0.27174394564916327 1109 1 0.1209642363229051 1109 2 0.3334388387874654 1109 3 -0.05449889821415965 1109 4 -0.07531109117372381 1109 5 -0.5294980750475204 1109 6 -0.753148457011303 1109 7 -8.787660372391315E-4 1109 8 -0.5650690095971669 1109 9 0.052926327492130554 1109 10-11 -0.27656694825537187 1109 12-13 0.5359394562187347 1109 14-15 -0.5044619396807661 1109 16-17 -0.23560980246293184 1109 18-19 -0.1865927761981503 1109 20-21 0.04339717225342454 1109 22-23 -0.534763109906681 1109 24-25 -0.060819447010910466 1109 26-27 0.30027830577013503 1109 28-29 0.24430093696331667 1109 30-31 -0.12253888383565581 1109 32-33 0.5602101049046375 1109 34-35 -0.01174908560355803 1109 36-37 0.2836855843906392 1109 38-39 -0.20346900044539495 1109 40-41 -0.6130379412964508 1109 42-43 -0.7593480522340812 1109 44-45 0.09483867107798716 1109 46-47 -0.410062372607058 1109 48-49 -0.3291365181455852 1109 50-51 -0.5435250962357294 1109 52-53 -0.27605653743668057 1109 54-55 0.16913847954501549 1109 56-57 -0.36352407959810407 1109 58-59 0.18277629361904957 1109 60-61 -0.519454105375079 1109 62-63 0.07875019566971275 1109 64-65 -0.1146217501410618 1109 66-67 -0.18901001018022612 1109 68-69 0.08315737369353116 1109 70-71 0.035745897466394894 1109 72-73 0.16950193632731825 1109 74-75 0.1353687615320247 1109 76 0.6027516975871485 1110 1 -0.21100910789034089 1110 2 -0.76886634277108 1110 3 0.025944458238988943 1110 4 -0.10036889071133004 1110 5 -0.1712089413605895 1110 6 0.17494211826409511 1110 7 -0.9544691373142697 1110 8 -0.7444175748086437 1110 9 -0.5363909579695836 1110 10-11 -0.35255634034023586 1110 12-13 -0.45899458430894313 1110 14-15 -0.24688000033627944 1110 16-17 -0.2855724027621278 1110 18-19 -0.6829038057099126 1110 20-21 -0.12743446109350742 1110 22-23 -0.4697726298305227 1110 24-25 -0.3467061599172112 1110 26-27 -1.1236473028249954 1110 28-29 -0.22989326948303201 1110 30-31 -0.2858395774301066 1110 32-33 -1.0283931862690316 1110 34-35 0.1447346625264565 1110 36-37 -0.5941193931695423 1110 38-39 0.0242951774411857 1110 40-41 0.4493515595875408 1110 42-43 0.32832024641952984 1110 44-45 -0.2855332859463573 1110 46-47 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-0.8334793952065951 2116 32-33 -1.3963619110275225 2116 34-35 -0.8699175296482693 2116 36-37 -0.8077958495037478 2116 38-39 -1.1787677284423097 2116 40-41 -1.113134305166426 2116 42-43 -0.6428923153716894 2116 44-45 -1.2676340258255188 2116 46-47 -0.7465328852628446 2116 48-49 -0.6798688835573792 2116 50-51 -0.6505050525507734 2116 52-53 -1.2190894296376307 2116 54-55 -0.7512489032122751 2116 56-57 -1.0579542479136634 2116 58-59 -1.2269628815062248 2116 60-61 -1.441158461021999 2116 62-63 -1.5257403606710298 2116 64-65 -1.5162021176086213 2116 66-67 -1.2751593099361038 2116 68-69 -0.6807190235825828 2116 70-71 -0.8740093383038712 2116 72-73 -1.329791395292883 2116 74-75 -0.35404727341593656 2116 76 -1.1598398480893266 2117 1 0.21983466821659192 2117 2 -0.789517971843761 2117 3 -0.6845985659882459 2117 4 0.2438583440422235 2117 5 0.15734577877307743 2117 6 0.7662203137528181 2117 7 1.0459318651919958 2117 8 0.549249927279913 2117 9 0.6033249985884765 2117 10-11 0.800476241113401 2117 12-13 0.7153746721655736 2117 14-15 -0.36240213901631435 2117 16-17 -0.5834337227287101 2117 18-19 0.12830755602776733 2117 20-21 -0.20439352542099343 2117 22-23 0.43472193992720776 2117 24-25 0.4086656028229747 2117 26-27 0.7315573755375766 2117 28-29 -0.25706118928252764 2117 30-31 0.8018797208155064 2117 32-33 0.18851575274517174 2117 34-35 0.2381014127353147 2117 36-37 0.38547960432418904 2117 38-39 0.2431422952356712 2117 40-41 0.3126431218264756 2117 42-43 0.12937287799847397 2117 44-45 0.03844332224077718 2117 46-47 0.15774493636304499 2117 48-49 0.04232527587434731 2117 50-51 0.7062589569204185 2117 52-53 0.38834545591880243 2117 54-55 -0.15865221812940078 2117 56-57 0.09611047189691035 2117 58-59 -0.481343307709853 2117 60-61 0.21039639296379065 2117 62-63 -0.02965511994490555 2117 64-65 0.14597625650345236 2117 66-67 0.6184318502848889 2117 68-69 -0.18876953660547002 2117 70-71 0.18971599713416865 2117 72-73 -1.083587764669364 2117 74-75 0.17425580472139757 2117 76 -0.05635129244607384 2118 1 0.4606952534145279 2118 2 0.3878658491545153 2118 3 0.8523377507071075 2118 4 -0.14584905699048178 2118 5 -0.06802427286203283 2118 6 0.5915559419800118 2118 7 0.06307472233485356 2118 8 -0.8482715873500375 2118 9 -0.23405882240979992 2118 10-11 -0.9269592149967565 2118 12-13 0.13953990452358056 2118 14-15 0.42493004687008806 2118 16-17 0.4164285836396182 2118 18-19 0.08620772814480304 2118 20-21 0.05982334204888673 2118 22-23 0.2313828693592228 2118 24-25 0.32882050815860353 2118 26-27 0.2915573755375789 2118 28-29 -0.3582487968556798 2118 30-31 -0.27944558038931433 2118 32-33 -0.2886271043976869 2118 34-35 -0.09275917246261756 2118 36-37 0.023878915856030858 2118 38-39 -0.26258920218257487 2118 40-41 0.4171525882636473 2118 42-43 -0.02171145935092511 2118 44-45 -0.06336390667488345 2118 46-47 -0.3783307951343744 2118 48-49 0.31838379566780617 2118 50-51 -0.15150352156495117 2118 52-53 -0.5082294149934192 2118 54-55 0.22170922765372936 2118 56-57 -0.2964884954008511 2118 58-59 -0.08564623369952784 2118 60-61 0.16760809692248202 2118 62-63 0.13261682497764227 2118 64-65 -0.09178622198191277 2118 66-67 0.21397401895958623 2118 68-69 -0.3284425142302574 2118 70-71 0.6508691812994023 2118 72-73 -0.3992848386796872 2118 74-75 0.014221381313475945 2118 76 -0.38709139571630047 2119 1 0.8319089216467788 2119 2 0.6750825124177382 2119 3 0.3591786737211997 2119 4 0.39601330772260823 2119 5 1.7736815334139173 2119 6 1.6224592160934108 2119 7 1.5150521234647982 2119 8 1.8944960935430224 2119 9 1.3572555877733024 2119 10-11 1.5029298327195377 2119 12-13 1.18095982147954 2119 14-15 1.4179529860845754 2119 16-17 1.5339835492648284 2119 18-19 1.3859831008219494 2119 20-21 1.0324426327710974 2119 22-23 1.1456501078045278 2119 24-25 1.7806107198528345 2119 26-27 0.9440997484189353 2119 28-29 1.4953520471985051 2119 30-31 0.8301283083861222 2119 32-33 1.1379265679187007 2119 34-35 1.4018463683446747 2119 36-37 1.749918668085293 2119 38-39 1.2782351120234026 2119 40-41 1.1816342436989515 2119 42-43 1.4584124260210771 2119 44-45 1.1214941696984049 2119 46-47 1.1837820630781408 2119 48-49 1.4920105058985627 2119 50-51 0.9820135977598063 2119 52-53 1.034398724683939 2119 54-55 0.7768280078593044 2119 56-57 1.0897343621309759 2119 58-59 0.8548955946307473 2119 60-61 1.5240848513980119 2119 62-63 0.9076491576983514 2119 64-65 0.9767591459304157 2119 66-67 0.7083753531097479 2119 68-69 0.8619972107714489 2119 70-71 0.5653576436232726 2119 72-73 1.4172354798826952 2119 74-75 1.2992598402339013 2119 76 1.2059247366096137 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 19.0 13 221.0 14 799.0 15 1540.0 16 2064.0 17 2512.0 18 2513.0 19 2290.0 20 1810.0 21 1285.0 22 895.0 23 758.0 24 605.0 25 606.0 26 601.0 27 659.0 28 714.0 29 852.0 30 1047.0 31 1240.0 32 1497.0 33 2019.0 34 2710.0 35 4042.0 36 7428.0 37 25579.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.69421612246437 19.39823542719252 11.8226378101199 34.08491064022321 2 18.253525375160244 18.75122539778297 34.45139883869995 28.543850388356834 3 17.92624990573863 20.031671819621447 30.996154136188824 31.0459241384511 4 14.591659754166352 22.02548827388583 28.20149310006787 35.18135887187995 5 17.019832591810573 31.014252318829648 30.91923685996531 21.046678229394463 6 28.352311288741422 29.261744966442954 16.660885302767515 25.72505844204811 7 25.838172083553278 27.92398763290853 19.206696327577106 27.03114395596109 8 22.518663750848354 33.39416333609833 18.16001809818264 25.927154814870672 9 22.562401025563684 18.196214463464294 18.651685393258425 40.58969911771359 10-11 25.469421612246435 23.05406832063947 23.820224719101123 27.656285348012972 12-13 23.952190634190483 19.85672272076012 24.040419274564513 32.15066737048488 14-15 24.05173063871503 21.05949777543172 21.959128270869467 32.929643314983785 16-17 25.196440690747302 22.65440012065455 22.166503280295604 29.98265590830254 18-19 24.28776110398914 21.07533368524244 23.386622426664655 31.25028278410376 20-21 24.121107005504864 21.749490988613225 23.538948797224947 30.590453208656964 22-23 25.962597089208963 21.621295528240704 22.29243646783802 30.123670914712314 24-25 24.62257748284443 23.223738782897218 21.530804615036573 30.62287911922178 26-27 23.860191539099613 23.45599879345449 23.099313777241534 29.58449589020436 28-29 24.117336550788025 22.436467838021265 25.084081140185504 28.3621144710052 30-31 24.40992383681472 21.383756881079858 25.102179322826334 29.10413995927909 32-33 23.275016967046223 23.17849332629515 24.659527939069452 28.88696176758917 34-35 23.428097428549883 22.5525978432999 24.786215217555238 29.23308951059498 36-37 23.74255335193424 20.989367317698516 25.608928436769475 29.65915089359777 38-39 22.495286931603953 20.863434130156097 28.135887187994875 28.505391750245078 40-41 22.108438277656287 20.668878666767213 28.16077218912601 29.061910866450496 42-43 20.46301183922781 20.99087549958525 28.13890355176834 30.4072091094186 44-45 19.455546338888468 20.395143654324713 30.39212729055124 29.75718271623558 46-47 19.727773169444234 22.150667370484882 29.732297715104444 28.389261744966444 48-49 19.784329990196817 22.672498303295377 28.79948721815851 28.743684488349295 50-51 19.587512253977827 23.764421989291908 27.212125782369355 29.43593997436091 52-53 18.357589925344996 24.105271095694143 27.681924440087474 29.855214538873387 54-55 18.637357665334438 22.856496493477113 27.816152628006936 30.68999321318151 56-57 19.318301787195537 23.388884699494756 27.037930774451397 30.254882738858306 58-59 19.737576351708018 23.241082874594674 26.126234823919763 30.89510594977754 60-61 19.305482241158284 22.128798733127216 26.73629439710429 31.82942462861021 62-63 19.678003167181963 22.385189653872256 26.348691652213258 31.588115526732523 64-65 20.07767136716688 23.029183319508334 25.429454792247945 31.463690521076842 66-67 19.51964406907473 24.46120202096373 23.71993062363321 32.299223286328335 68-69 19.782821808310082 25.793680717894578 23.463539702888166 30.95995777090717 70-71 19.380137244551694 26.29213483146067 24.545660206620916 29.78206771736672 72-73 19.68478998567227 25.188145690370256 26.094563004298315 29.032501319659147 74-75 19.136565869843903 27.316190332554108 25.222833873765175 28.324409923836814 76 18.42394992836136 26.721966669180304 24.002714727396125 30.85136867506221 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.5 14 2.5 15 4.5 16 7.0 17 7.0 18 9.5 19 12.5 20 16.0 21 19.0 22 26.0 23 44.5 24 65.0 25 74.0 26 103.5 27 162.5 28 237.0 29 282.0 30 371.5 31 503.5 32 655.5 33 765.0 34 849.5 35 1094.5 36 1358.0 37 1461.0 38 1599.5 39 1976.5 40 2370.5 41 2662.0 42 2798.0 43 2930.0 44 3113.0 45 3200.5 46 3237.0 47 3202.5 48 3041.0 49 2808.0 50 2702.0 51 2569.0 52 2251.5 53 2041.0 54 2015.0 55 1951.0 56 1881.0 57 1871.0 58 1867.0 59 1873.5 60 1906.0 61 1995.5 62 2059.0 63 2107.5 64 2049.5 65 1943.0 66 1958.5 67 1974.0 68 1795.0 69 1585.0 70 1375.0 71 1196.0 72 1028.5 73 741.5 74 477.5 75 333.0 76 235.5 77 96.5 78 37.5 79 20.0 80 16.0 81 9.0 82 7.0 83 8.0 84 7.5 85 4.5 86 3.0 87 4.0 88 3.5 89 3.5 90 6.5 91 7.5 92 6.0 93 3.0 94 2.0 95 5.0 96 6.0 97 4.5 98 3.5 99 11.0 100 18.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 66305.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.12849709674987 #Duplication Level Percentage of deduplicated Percentage of total 1 82.12056976444717 62.51715556896161 2 11.379440141054342 17.325993514817885 3 3.5105097371079896 8.017494909886132 4 1.418467817025576 4.319432923610587 5 0.6597064009350794 2.5111228414146747 6 0.35857915486261066 1.6378855289947967 7 0.21197773243259305 1.1296282331649197 8 0.11292271727717575 0.6877309403514064 9 0.08914951363987558 0.6108136641278938 >10 0.13867702121758424 1.2427418746700851 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0015081818867355404 0.0 2 0.0 0.0 0.0 0.0015081818867355404 0.0 3 0.0 0.0 0.0 0.010557273207148781 0.0 4 0.0 0.0 0.0 0.06786818490309932 0.0 5 0.0 0.0 0.0 0.07390091245004148 0.0 6 0.0 0.0 0.0 0.07390091245004148 0.0 7 0.0 0.0 0.0 0.07540909433677702 0.0 8 0.0 0.0 0.0 0.07540909433677702 0.0 9 0.0 0.0 0.0 0.09049091320413241 0.0 10 0.0 0.0 0.0 0.09954000452454566 0.0 11 0.0 0.0 0.0 0.10255636829801675 0.0 12 0.0 0.0 0.0 0.10557273207148782 0.0 13 0.0 0.0 0.0 0.10557273207148782 0.0 14 0.0 0.0 0.0 0.11160545961842998 0.0 15 0.0 0.0 0.0 0.12367091471231431 0.0 16 0.0 0.0 0.0 0.12819546037252091 0.0 17 0.0 0.0 0.0 0.12970364225925646 0.0 18 0.0 0.0 0.0 0.14026091546640526 0.0 19 0.0 0.0 0.0 0.15232637056028958 0.0 20 0.0 0.0 0.0 0.15835909810723173 0.0 21 0.0 0.0 0.0 0.167408189427645 0.0 22 0.0 0.0 0.0 0.18249000829500037 0.0 23 0.0 0.0 0.0 0.18852273584194254 0.0 24 0.0 0.0 0.0 0.19908000904909132 0.0 25 0.0 0.0 0.0 0.21114546414297564 0.0 26 0.0 0.0 0.0 0.23226001055727322 0.0 27 0.0 0.0 0.0 0.36498001659000073 0.0 28 0.0 0.0 0.0 0.5112736596033481 0.0 29 0.0 0.0 0.0 0.6651082120503733 0.0 30 0.0 0.0 0.0 0.8068773094035141 0.0 31 0.0 0.0 0.0 0.9788100444913657 0.0 32 0.0 0.0 0.0 1.1884473267476057 0.0 33 0.0 0.0 0.0 1.5006409773018625 0.0 34 0.0 0.0 0.0 1.9199155418143428 0.0 35 0.0 0.0 0.0 2.5171555689616167 0.0 36 0.0 0.0 0.0 3.3451474247794284 0.0 37 0.0 0.0 0.0 4.405399291154513 0.0 38 0.0 0.0 0.0 5.446044793002036 0.0 39 0.0 0.0 0.0 6.3675439257974515 0.0 40 0.0 0.0 0.0 7.399140336324561 0.0 41 0.0 0.0 0.0 8.391524017796547 0.0 42 0.0 0.0 0.0 9.326596787572582 0.0 43 0.0 0.0 0.0 10.278259558102707 0.0 44 0.0 0.0 0.0 11.157529598069527 0.0 45 0.0 0.0 0.0 12.036799638036348 0.0 46 0.0 0.0 0.0 13.074428776110398 0.0 47 0.0 0.0 0.0 13.962747907397633 0.0 48 0.0 0.0 0.0 14.787723399441973 0.0 49 0.0 0.0 0.0 15.574994344317926 0.0 50 0.0 0.0 0.0 16.315511650705076 0.0 51 0.0 0.0 0.0 17.031898046904455 0.0 52 0.0 0.0 0.0 17.816152628006936 0.0 53 0.0 0.0 0.0 18.537063569866525 0.0 54 0.0 0.0 0.0 19.239876329085288 0.0 55 0.0 0.0 0.0 19.957770907171405 0.0 56 0.0 0.0 0.0 20.627403664881985 0.0 57 0.0 0.0 0.0 21.24123369278335 0.0 58 0.0 0.0 0.0 21.89729281351331 0.0 59 0.0 0.0 0.0 22.50961465952794 0.0 60 0.0 0.0 0.0 23.112887414222154 0.0 61 0.0 0.0 0.0 23.74029107910414 0.0 62 0.0 0.0 0.0 24.360153834552445 0.0 63 0.0 0.0 0.0 24.93778749717216 0.0 64 0.0 0.0 0.0 25.500339340924516 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTACGC 15 0.002215644 70.0 20 CACCTTA 15 0.002215644 70.0 53 TCTATCA 20 7.87373E-5 70.0 10 GCGAGAC 20 0.006923323 52.5 60 GGTACTG 20 0.006923323 52.5 5 TACCCGA 20 0.006923323 52.5 14 ACCCGAT 20 0.006923323 52.5 15 CTTTATC 20 0.006923323 52.5 40 TAGTAGC 20 0.006923323 52.5 64 CCCGAAG 20 0.006923323 52.5 12 CTTATAA 45 1.6616996E-6 46.666664 1 TACTCTA 30 5.8391894E-4 46.666664 7 GGTCTTT 35 0.0012472312 40.0 35 CTTAACA 55 2.9633474E-4 31.81818 1 TTATAAA 55 2.9633474E-4 31.81818 2 TATTTTC 45 0.0042795227 31.111109 25 AGTGCTT 45 0.0042795227 31.111109 42 CTTATAT 45 0.0042795227 31.111109 1 TGTACCC 45 0.0042795227 31.111109 12 TATCCAC 45 0.0042795227 31.111109 3 >>END_MODULE