FastQCFastQC Report
Wed 25 May 2016
SRR1780270_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780270_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66305
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT783811.821129628233166No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG19412.9273810421536837No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC19072.8761028580046757No Hit
CACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTG18522.7931528542342208RNA PCR Primer, Index 47 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC4580.6907473041248774No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT2500.3770454716838851Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT2410.3634718347032652No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1340.2020963728225624No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT1230.18550637206847145No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC840.1266872784857854No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG740.11160545961842999No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCG740.11160545961842999No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC720.10858909584495889No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT800.061.251
TTTATAC1000.055.9999963
CTTTATA900.054.4444432
TCCATGC200.00692332352.533
AGTCCGA200.00692332352.517
AAAAGAC200.00692332352.569
GGACAAG200.00692332352.51
GCCGCCA350.001247231240.035
ACTCGTA350.001247231240.038
AACTCGT350.001247231240.037
CTGCTTG17000.035.61764554
TCTGCTT17050.035.513253
TCTTCTG17050.035.3079250
TTCTGCT17200.035.20348752
TGCTTGA17050.035.1026455
GTCTTCT17150.035.1020449
CGTCTTC17250.035.1014548
TCGTATG17350.035.10086440
CGTATGC17300.035.041
CTCGTAT17300.035.039