FastQCFastQC Report
Wed 25 May 2016
SRR1780269_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780269_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385915
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATAAG250.0023554435.18589430
GCGATTT353.1799672E-431.4853720
CGATAGT300.005751479429.32157928
AGAGACA1006.184564E-1126.3723246
GAAAATT858.232746E-925.8719826
TAAGAGA1200.025.6397614
ATTCTTA450.001381871224.48862315
ATAGTAT450.001399754224.43464930
ATAAGAG1350.024.4188183
GTATAAG1202.3646862E-1123.808351
CTTACTC500.002587180121.98548123
TATAAGA1451.2732926E-1121.2191122
TTACTCG654.8641767E-420.34439519
AAGAGAC1301.6352715E-920.2864045
AGCGACC550.004468397220.0075512
CAATCTA550.004498686619.98421333
CCCAATG708.131516E-418.84958530
TAGATAG708.167033E-418.8373765
TAATGAT600.00732645118.36646820
TCAGTGG600.007420316318.32598729
Produced by FastQC (version 0.11.5)