FastQCFastQC Report
Wed 25 May 2016
SRR1780269_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780269_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385915
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT221615.74245624036381No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG58531.5166552220048457No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC57791.4974800150292162No Hit
CACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTTCTG42161.0924685487736938RNA PCR Primer, Index 14 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC13320.3451537255613283No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT6300.16324838371143904RNA PCR Primer, Index 35 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG5150.038.44388644
GTCTTCT5250.037.7116243
TCTTTAT1550.035.5504571
GTTCTCT250.0023516335.1975146
CGCCATT250.0023516335.19751433
CGTCTTC5950.033.6446842
ATGCCGT30100.033.25346444
CTCTTGC353.2078443E-431.438572
CTTTATA1700.031.0687052
GCTTATA502.3501161E-630.8098013
CCGTCTT6450.030.69550541
ATCGTAT300.005742214629.33125939
ATATCGT300.005742214629.33125937
TACGACA300.005742214629.33125939
TTTTCGG300.005742214629.33125926
TATGCCG36500.027.4227243
GCCGTCT7450.026.57530440
TCTTGCT508.308984E-526.40843
CAGCTTA609.589972E-625.7148281
AGCGTCA701.1258035E-625.1508563
Produced by FastQC (version 0.11.5)