FastQCFastQC Report
Wed 25 May 2016
SRR1780268_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780268_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124072
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGCAA200.006937552.51
CGGTATG305.859032E-446.66666843
CCCGCAA305.859032E-446.6666681
CACTAAG404.5710272E-543.7536
GTCGTAA350.001251443940.021
GGCGGTA350.001251443940.041
GCGGTAT400.002411151335.042
TAAACAG400.002411151335.037
CCAGATT400.002411151335.018
ATGCACT400.002411151335.047
ATAAGAG652.08902E-532.3076933
CATCTTA552.9781938E-431.81818410
GAGTGTG450.004293803631.1111097
CTATGAA450.004293803631.1111092
CTTTTGG450.004293803631.11110936
ACAATCC450.004293803631.11110916
ACGCTTC450.004293803631.11110915
GGTCTAG450.004293803631.1111091
AACAGTC450.004293803631.11110957
CTTGTTC500.007185987727.9999983