FastQCFastQC Report
Wed 25 May 2016
SRR1780268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780268_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124072
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT71525.764394867496293No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC20391.643400606099684No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG18781.5136372428912244No Hit
CACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTTCTG16471.3274550261138693RNA PCR Primer, Index 18 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC4710.3796182861564253No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT2450.1974659874911342No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT2220.17892836417563995RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT1800.14507705203430266No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTC150.002220215770.0000127
CACTGAG150.002220215770.000017
GAAAGCG150.002220215770.0000124
ATCCCAA150.002220215770.0000159
CGACTGG150.002220215770.0000135
AAAGCGT150.002220215770.0000125
GCTTATA308.336288E-658.3333363
TCTTTAT601.8189894E-1258.3333361
GAAGCGC200.006937552.521
AAGCGTT200.006937552.526
ATAGACC200.006937552.514
CACTACC200.006937552.546
ACCAATT200.006937552.515
TCAAGCT200.006937552.532
ATCATGT200.006937552.515
CTTTATA707.2759576E-1250.02
ATCGTAT305.859032E-446.66666839
GATATCG305.859032E-446.66666836
TATCCTG305.859032E-446.66666838
TTTATAC1150.042.60873
Produced by FastQC (version 0.11.5)