##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780268_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 124072 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.74871042620414 34.0 34.0 34.0 34.0 34.0 2 33.720275324005414 34.0 34.0 34.0 34.0 34.0 3 33.73004384550906 34.0 34.0 34.0 34.0 34.0 4 33.75548874846863 34.0 34.0 34.0 34.0 34.0 5 33.74105358179122 34.0 34.0 34.0 34.0 34.0 6 37.49668740731188 38.0 38.0 38.0 37.0 38.0 7 37.48964311045199 38.0 38.0 38.0 37.0 38.0 8 37.40970565478109 38.0 38.0 38.0 37.0 38.0 9 37.41650815655426 38.0 38.0 38.0 37.0 38.0 10-11 37.371163517957314 38.0 38.0 38.0 37.0 38.0 12-13 37.36067364111162 38.0 38.0 38.0 37.0 38.0 14-15 37.27676268618222 38.0 38.0 38.0 36.0 38.0 16-17 37.309296215100915 38.0 38.0 38.0 36.0 38.0 18-19 37.27509430008382 38.0 38.0 38.0 36.0 38.0 20-21 37.296001515249216 38.0 38.0 38.0 36.0 38.0 22-23 37.28932394093752 38.0 38.0 38.0 36.0 38.0 24-25 37.30496808304855 38.0 38.0 38.0 36.0 38.0 26-27 37.18184602488877 38.0 38.0 38.0 36.0 38.0 28-29 37.15344316203495 38.0 38.0 38.0 36.0 38.0 30-31 37.17121913082726 38.0 38.0 38.0 36.0 38.0 32-33 37.05864739828487 38.0 38.0 38.0 36.0 38.0 34-35 37.111499774324585 38.0 38.0 38.0 36.0 38.0 36-37 37.07159955509704 38.0 38.0 38.0 36.0 38.0 38-39 36.32571410148946 38.0 37.0 38.0 34.0 38.0 40-41 36.702575923657236 38.0 37.0 38.0 34.5 38.0 42-43 37.02816509768522 38.0 38.0 38.0 36.0 38.0 44-45 37.10083258108195 38.0 38.0 38.0 36.0 38.0 46-47 37.04429282996969 38.0 38.0 38.0 36.0 38.0 48-49 37.068613385776004 38.0 38.0 38.0 36.0 38.0 50-51 36.91837400864014 38.0 38.0 38.0 35.0 38.0 52-53 36.91621397253208 38.0 38.0 38.0 35.0 38.0 54-55 36.895129440969754 38.0 38.0 38.0 35.5 38.0 56-57 36.945684763685605 38.0 38.0 38.0 36.0 38.0 58-59 36.838198787800636 38.0 37.5 38.0 35.0 38.0 60-61 36.840354793990585 38.0 38.0 38.0 35.0 38.0 62-63 36.8310738925785 38.0 37.5 38.0 35.0 38.0 64-65 36.76425785028048 38.0 37.5 38.0 34.5 38.0 66-67 36.50220839512541 38.0 37.5 38.0 34.0 38.0 68-69 36.41299406796054 38.0 37.0 38.0 34.0 38.0 70-71 35.80650348184925 38.0 37.0 38.0 31.0 38.0 72-73 34.76682490811787 38.0 37.0 38.0 24.0 38.0 74-75 34.43210796956606 38.0 36.5 38.0 22.5 38.0 76 33.727529176607135 38.0 34.0 38.0 21.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.07352500003400309 1101 2 -0.1165480880283738 1101 3 -0.2075470508113284 1101 4 -0.024580562944478856 1101 5 -0.11173967271841434 1101 6 -0.12576119577152411 1101 7 6.836696780894158E-4 1101 8 -0.13269887885314802 1101 9 -0.09749671008363237 1101 10-11 0.20875317912929603 1101 12-13 0.16409590541735497 1101 14-15 0.3042466031712294 1101 16-17 0.16134734220516123 1101 18-19 -0.036927846529749786 1101 20-21 0.08599527621997538 1101 22-23 0.03180211954342127 1101 24-25 -0.13643623570988694 1101 26-27 -0.1835155291745565 1101 28-29 -0.037676205645645666 1101 30-31 0.03426849232668161 1101 32-33 -0.08829333225070002 1101 34-35 0.034212972908697736 1101 36-37 -0.034297931187033726 1101 38-39 -0.01621352118348085 1101 40-41 0.06110386608899887 1101 42-43 0.17272064166595413 1101 44-45 0.05230754265477344 1101 46-47 0.07592425854940643 1101 48-49 -0.024126330760687154 1101 50-51 -0.10944955681890889 1101 52-53 -0.04113017135647823 1101 54-55 -0.17523569727173793 1101 56-57 -0.14498181323882875 1101 58-59 -0.08859215489940198 1101 60-61 -0.25111594044970076 1101 62-63 -0.33524816264252877 1101 64-65 -0.033832869976890834 1101 66-67 -0.3443468932565281 1101 68-69 -0.6988041316391644 1101 70-71 -0.4477423611289595 1101 72-73 -0.22343122987314956 1101 74-75 0.27811968852316227 1101 76 -0.46031842759700936 1102 1 0.03178631163982715 1102 2 -0.026561716551064762 1102 3 -0.24742239271569844 1102 4 -0.1506586934542682 1102 5 -0.002206378142183496 1102 6 -0.07135193981262233 1102 7 -0.0438997073364078 1102 8 -0.24031655590481193 1102 9 -0.40375725312784283 1102 10-11 -0.12992990530082693 1102 12-13 0.11955541536236325 1102 14-15 0.14545525299443085 1102 16-17 -0.09307239723273852 1102 18-19 0.036013341153115164 1102 20-21 0.05623172632337514 1102 22-23 -0.01124209214660965 1102 24-25 -0.14364124490675323 1102 26-27 -0.12564672514889708 1102 28-29 -0.029618222476393896 1102 30-31 0.17820665886150522 1102 32-33 -0.020493814490734508 1102 34-35 0.18430780074137942 1102 36-37 -0.060935498257471465 1102 38-39 0.4281287358142407 1102 40-41 0.25247024461883427 1102 42-43 0.23813278534679938 1102 44-45 0.11021446842220683 1102 46-47 0.1214368530198513 1102 48-49 0.10407232163574776 1102 50-51 0.057590385236039765 1102 52-53 0.19152681874140143 1102 54-55 -0.0339184004844455 1102 56-57 0.1439476329475724 1102 58-59 -0.05539288302467327 1102 60-61 0.12185765071367882 1102 62-63 0.11425959036670008 1102 64-65 0.2786701082841887 1102 66-67 0.2829130112485103 1102 68-69 0.41497020671035756 1102 70-71 0.5839463367276423 1102 72-73 0.6584819097381924 1102 74-75 0.4686045017742799 1102 76 0.273010458199785 1103 1 -0.010545514098922126 1103 2 0.0955176913754272 1103 3 -0.16552961665605892 1103 4 -0.12250302388562773 1103 5 -0.04995481840747118 1103 6 0.014813347375209673 1103 7 0.044661046341708754 1103 8 0.11454320204581592 1103 9 -0.04293564387490534 1103 10-11 -0.05354312684572449 1103 12-13 0.14082858792533415 1103 14-15 -0.027380335771901798 1103 16-17 -0.05187573658453459 1103 18-19 0.004389608158248848 1103 20-21 0.1443455646115126 1103 22-23 0.09357297426501532 1103 24-25 0.033453447526071045 1103 26-27 -0.0911135729652699 1103 28-29 -0.04285138717365555 1103 30-31 -0.08713594647644385 1103 32-33 -0.15290143707962756 1103 34-35 -0.20684595602939027 1103 36-37 -0.03496524494583042 1103 38-39 -0.6704563300610857 1103 40-41 -0.0032055808338924408 1103 42-43 -0.15353045496854634 1103 44-45 -0.001684206920572251 1103 46-47 -0.1552976256086609 1103 48-49 -0.0776747600067651 1103 50-51 -0.2827178076168906 1103 52-53 -0.008543793890218865 1103 54-55 -0.4750462361625907 1103 56-57 -0.361835452198747 1103 58-59 -0.15151097594732477 1103 60-61 -0.18253195224669128 1103 62-63 0.019576095845884822 1103 64-65 -0.18277450102237935 1103 66-67 -0.37190741814779216 1103 68-69 -0.2106711168742592 1103 70-71 -0.41302804608220356 1103 72-73 -0.7511830824854755 1103 74-75 -0.6979876789940604 1103 76 -0.8209758483126635 1104 1 0.010791587375948097 1104 2 0.09968619795495925 1104 3 0.03646632081633783 1104 4 -0.01800774878185507 1104 5 0.01887852964516412 1104 6 0.06702565860015142 1104 7 -0.07002587408574357 1104 8 -0.2898814204040647 1104 9 -0.2127846238272184 1104 10-11 -0.004439532391963041 1104 12-13 -0.2935527597740375 1104 14-15 -0.25653780287426287 1104 16-17 -0.1823270304112583 1104 18-19 -0.028914286569303727 1104 20-21 -0.20661266200687578 1104 22-23 -0.08641907731141885 1104 24-25 0.023067507404199716 1104 26-27 0.03349562954289809 1104 28-29 -0.12829252468138463 1104 30-31 -0.1457776492054066 1104 32-33 -0.10600132226906567 1104 34-35 -0.011999183972513094 1104 36-37 -0.2717620302500805 1104 38-39 0.009456237279728441 1104 40-41 -0.37788102020508063 1104 42-43 -0.31582401674545224 1104 44-45 -0.23681623906753657 1104 46-47 -0.23704716195062048 1104 48-49 -0.0751268486758434 1104 50-51 -0.3460755749930229 1104 52-53 -0.067746302059426 1104 54-55 -0.17865393730186696 1104 56-57 -0.5523503334139761 1104 58-59 -0.05729124619372783 1104 60-61 -0.23466036344512986 1104 62-63 -0.2893702964529723 1104 64-65 -0.35261906518381636 1104 66-67 -0.5530431595590883 1104 68-69 -0.30778760543736183 1104 70-71 -0.25076981063223514 1104 72-73 0.21632111251584973 1104 74-75 0.001341120793988182 1104 76 -0.23704049549097306 1105 1 -0.11322879410035114 1105 2 0.10334764900785132 1105 3 0.03958667072841138 1105 4 -0.005643587714082798 1105 5 0.13978757956174093 1105 6 0.09481120218134009 1105 7 0.15247491502008614 1105 8 0.1494115407719292 1105 9 0.0977533208902841 1105 10-11 0.13442054787137891 1105 12-13 -0.0919249471754 1105 14-15 -0.19442390108411445 1105 16-17 -0.029628288471407416 1105 18-19 0.09945744991443917 1105 20-21 -0.04346615887299521 1105 22-23 0.05220201661472146 1105 24-25 -0.18291616937805344 1105 26-27 0.1492777461501973 1105 28-29 0.22868993462329712 1105 30-31 0.02714925704881921 1105 32-33 0.1305635873219586 1105 34-35 0.17071263457823704 1105 36-37 0.11278051382711851 1105 38-39 -0.4313878804999547 1105 40-41 -0.13121555598539203 1105 42-43 -0.1485741632936879 1105 44-45 0.08302413609592918 1105 46-47 0.1320108711467327 1105 48-49 -0.2962297931678819 1105 50-51 -0.35479632171259823 1105 52-53 -0.2752405528598061 1105 54-55 -0.23029302284093234 1105 56-57 -0.010130916901374576 1105 58-59 -0.06898804918781565 1105 60-61 -0.017115158953998844 1105 62-63 0.10217499822168463 1105 64-65 0.12610213245336865 1105 66-67 -0.14155828784515023 1105 68-69 0.1747889378281826 1105 70-71 -0.0036669563237197167 1105 72-73 -0.06357247092645224 1105 74-75 -0.20813265834656391 1105 76 -0.017019753280578698 1106 1 0.08784941202515739 1106 2 -0.020664146132986616 1106 3 -0.015155808060235643 1106 4 -0.02766902324785292 1106 5 -0.017782476700354266 1106 6 -0.16486280328205538 1106 7 -0.1825649749884164 1106 8 -0.08897874813666107 1106 9 0.12313458253617426 1106 10-11 0.0026433168349626612 1106 12-13 0.10482958002398135 1106 14-15 -0.007990122116375176 1106 16-17 0.0037083614915047747 1106 18-19 -0.19434015125783333 1106 20-21 -0.08947082627535252 1106 22-23 -0.08575771347193495 1106 24-25 0.08842634197386445 1106 26-27 -0.028761944860534072 1106 28-29 -0.07577666088658219 1106 30-31 -0.1590719561139835 1106 32-33 -0.21692723164434824 1106 34-35 -0.20176145048426264 1106 36-37 0.0031508305953948934 1106 38-39 0.13451656438670767 1106 40-41 -0.09857992091113488 1106 42-43 0.008172765808836857 1106 44-45 -0.22681087665802124 1106 46-47 -0.18677904147515534 1106 48-49 -0.07077435962408885 1106 50-51 -0.07652959233148238 1106 52-53 0.09342091574377065 1106 54-55 0.1992708102180174 1106 56-57 0.059531872323802304 1106 58-59 -0.06393169663611786 1106 60-61 -0.1354781308953079 1106 62-63 0.0400469883803396 1106 64-65 -0.01170834930215392 1106 66-67 0.09643400551375692 1106 68-69 0.27172799023695404 1106 70-71 0.1457803725834168 1106 72-73 0.03769478308516483 1106 74-75 0.0680807758123052 1106 76 -0.09794853016789062 1107 1 0.10378118484315735 1107 2 -0.15784433121736186 1107 3 -0.003560816225721908 1107 4 0.08158519558720911 1107 5 0.07880616419966202 1107 6 0.08443859392353659 1107 7 0.13924594861892103 1107 8 0.20862604228250348 1107 9 0.1992913598905588 1107 10-11 -0.02617178024360811 1107 12-13 0.017957868900772667 1107 14-15 0.20732470277928883 1107 16-17 -0.05602671381242885 1107 18-19 -0.13576638969006183 1107 20-21 -0.0016827660322036309 1107 22-23 0.13359449120960676 1107 24-25 0.15533797325675636 1107 26-27 0.16085881362250376 1107 28-29 0.04656048300744686 1107 30-31 -0.16153974780332447 1107 32-33 0.143252409074222 1107 34-35 -0.011705199350167561 1107 36-37 0.1103727503921661 1107 38-39 0.0953675896089905 1107 40-41 0.11736999743399679 1107 42-43 0.04955364042324106 1107 44-45 0.11381695858058549 1107 46-47 -0.12659940075658938 1107 48-49 -0.053100616201845696 1107 50-51 0.17708136756689896 1107 52-53 0.05997870924766602 1107 54-55 0.2353894819654343 1107 56-57 -0.06343129225612643 1107 58-59 0.11061920156747362 1107 60-61 -0.052435125996012744 1107 62-63 -0.0708040368419347 1107 64-65 0.27714122427796184 1107 66-67 -0.005689021160172558 1107 68-69 0.26885192418723847 1107 70-71 0.0802568741257943 1107 72-73 0.17599999085144447 1107 74-75 0.3678721022503382 1107 76 0.5213946017500959 1108 1 -0.15379457273260044 1108 2 0.011852638479609823 1108 3 -0.06719717986208451 1108 4 -0.06386934986733905 1108 5 -0.07876959337609435 1108 6 -0.4549553454704096 1108 7 -0.2031782938053226 1108 8 -0.20955577589931806 1108 9 0.029594390193587117 1108 10-11 -0.10647481054663643 1108 12-13 -0.08052697958407862 1108 14-15 0.05580955126414011 1108 16-17 0.11518540739968586 1108 18-19 -0.09788760181127287 1108 20-21 -0.05925852360795858 1108 22-23 0.06238236999749347 1108 24-25 0.10533797325674499 1108 26-27 -0.010353307589618055 1108 28-29 0.05413624058319755 1108 30-31 0.0066420703784899615 1108 32-33 -0.03402031819850748 1108 34-35 -0.01473550238047494 1108 36-37 0.17097881099822843 1108 38-39 -0.009177864936468438 1108 40-41 0.1461578762218778 1108 42-43 0.005614246483851559 1108 44-45 0.17896847373210534 1108 46-47 0.356733932576752 1108 48-49 -0.006130919232148813 1108 50-51 0.11799045847599388 1108 52-53 -0.01729401802506203 1108 54-55 -0.10854991197395947 1108 56-57 -0.22403735286218307 1108 58-59 -0.13332019237192583 1108 60-61 -0.2645563381172309 1108 62-63 -0.20262221866012453 1108 64-65 0.14683819397492925 1108 66-67 0.08522006974892093 1108 68-69 0.06733677267209259 1108 70-71 0.28934778321669796 1108 72-73 0.35781817266963145 1108 74-75 -0.21091577653754 1108 76 0.7365461169016072 1109 1 -0.013530908903980787 1109 2 0.10334764900785132 1109 3 -0.005730549815396557 1109 4 0.042694780865979 1109 5 0.009878214002831953 1109 6 -0.08343653195763778 1109 7 0.010480957316161721 1109 8 0.08596743201059809 1109 9 0.1702608737603768 1109 10-11 0.0030006082943359047 1109 12-13 0.012304660075351137 1109 14-15 0.05179966387056112 1109 16-17 0.0972599290512477 1109 18-19 0.09492572786005837 1109 20-21 0.03659426408772504 1109 22-23 -0.056559312690417585 1109 24-25 0.07539198772163758 1109 26-27 -0.03803343209753507 1109 28-29 -0.2335457149235296 1109 30-31 -0.06348518403878955 1109 32-33 -0.03711012869013075 1109 34-35 -0.07702150439457256 1109 36-37 0.2638379156398045 1109 38-39 0.248370427657143 1109 40-41 0.039479308062944085 1109 42-43 0.13541375211417517 1109 44-45 0.18423259531042646 1109 46-47 0.04137643005912395 1109 48-49 0.049691656983178234 1109 50-51 0.10441817979797463 1109 52-53 0.09182893354502397 1109 54-55 0.01895169014999709 1109 56-57 0.19530714956388806 1109 58-59 0.08660107467925116 1109 60-61 0.034244357662316816 1109 62-63 -0.15764373289613332 1109 64-65 -0.020423547304929457 1109 66-67 -0.14609000989952392 1109 68-69 -0.08049807123526165 1109 70-71 -0.01575154846872806 1109 72-73 -0.1104002654883871 1109 74-75 0.13929936582261604 1109 76 0.7110769234565808 1110 1 0.01016454507267639 1110 2 -0.19146455714528088 1110 3 -0.12115091843121206 1110 4 0.007674490907270126 1110 5 0.08022269638542667 1110 6 -0.26625174138218455 1110 7 -0.27190700099556864 1110 8 -0.10439170919678986 1110 9 -0.11985872079798554 1110 10-11 -0.12011117418300188 1110 12-13 -0.1239643114829505 1110 14-15 -0.32175903399674866 1110 16-17 -0.1687396419099727 1110 18-19 -0.2811133504216343 1110 20-21 -0.10713372532931942 1110 22-23 -0.28660454052788253 1110 24-25 -0.27423886016623555 1110 26-27 -0.29821416468124085 1110 28-29 -0.2972892569961445 1110 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54-55 0.009888246041235504 2115 56-57 0.15603222509258785 2115 58-59 -0.02518140266214175 2115 60-61 0.17774888938437527 2115 62-63 0.14749221876549257 2115 64-65 0.10495409619959162 2115 66-67 0.07294322272887399 2115 68-69 0.08566507075869367 2115 70-71 -0.16076665420891345 2115 72-73 0.33975079191342417 2115 74-75 0.21633864074708953 2115 76 0.055487799589514 2116 1 0.07225864234500534 2116 2 0.028408440401264556 2116 3 0.040452487543682025 2116 4 0.016784752128224056 2116 5 -0.16927218022014046 2116 6 0.005576805305643973 2116 7 0.18266797374825927 2116 8 0.17874799350201442 2116 9 -0.03784832968815266 2116 10-11 0.05366931362187444 2116 12-13 -0.07886400840891383 2116 14-15 0.0989636532597089 2116 16-17 -0.36083085276291627 2116 18-19 -0.05106572450159064 2116 20-21 -0.04235164999376906 2116 22-23 0.08140491249564263 2116 24-25 -0.08117348276689995 2116 26-27 0.0333181632159949 2116 28-29 -0.21279834921724472 2116 30-31 -0.08505231247442424 2116 32-33 -0.09333295677943454 2116 34-35 -0.027743632461778134 2116 36-37 -0.12466974776008755 2116 38-39 -0.009168626207717523 2116 40-41 -0.3854847697500361 2116 42-43 -0.15270060939198515 2116 44-45 -0.16702191798999877 2116 46-47 0.12583962363365941 2116 48-49 -0.007516728544786133 2116 50-51 0.07221255751516509 2116 52-53 -0.23296290198662462 2116 54-55 0.02740722032464049 2116 56-57 -0.11155583031968774 2116 58-59 0.17738749425039657 2116 60-61 -0.31122115703814757 2116 62-63 0.041963886291839 2116 64-65 -0.42868841356386866 2116 66-67 -0.03786751339963956 2116 68-69 -0.03520387773441769 2116 70-71 0.4722007026549875 2116 72-73 0.2570883130983077 2116 74-75 0.2926134178453097 2116 76 0.2422186963546764 2117 1 0.010657641719618027 2117 2 -0.030221953594988804 2117 3 -0.164050326715234 2117 4 -0.06357797457596348 2117 5 -0.10814022274671942 2117 6 -0.011074226589286695 2117 7 0.06611075048371617 2117 8 -0.567656008999549 2117 9 -0.04762006202086155 2117 10-11 0.19399138991956022 2117 12-13 0.10988677332967 2117 14-15 0.050652208606798865 2117 16-17 0.12255720227148714 2117 18-19 0.07909375017007392 2117 20-21 0.060642408792972446 2117 22-23 0.17185206696719035 2117 24-25 0.012127017545800811 2117 26-27 0.23457676234045266 2117 28-29 -0.19262949368253146 2117 30-31 0.17432855056497232 2117 32-33 -0.031106565284758858 2117 34-35 0.06250808736311342 2117 36-37 -0.09746524494583042 2117 38-39 -0.340821221579823 2117 40-41 -0.2942558766918779 2117 42-43 0.010917276786884145 2117 44-45 0.28837989564352995 2117 46-47 0.25224675308956535 2117 48-49 -0.028858191959422186 2117 50-51 0.11915595964149617 2117 52-53 0.2033644901334526 2117 54-55 0.33807013464608815 2117 56-57 0.12226218093734076 2117 58-59 0.263183304131573 2117 60-61 -0.05703885559974253 2117 62-63 0.020192466654563646 2117 64-65 -0.223208426071686 2117 66-67 0.007160629189478129 2117 68-69 -0.04067604784073353 2117 70-71 0.04185361072252647 2117 72-73 -0.37088229353084046 2117 74-75 -0.5733575014792578 2117 76 -0.47580819545270714 2118 1 -0.035896845459873816 2118 2 -0.13767387667191144 2118 3 0.018882365592460815 2118 4 -0.00989207714006568 2118 5 -0.06759535095184788 2118 6 0.0021469272568666042 2118 7 0.043033827406794956 2118 8 0.14612604228250348 2118 9 0.31520045079965087 2118 10-11 0.025201325817000964 2118 12-13 0.02018324768864943 2118 14-15 0.15041182399141206 2118 16-17 0.23653957406635584 2118 18-19 -0.1969879805991539 2118 20-21 -0.08042329633523337 2118 22-23 0.04424790030051895 2118 24-25 0.22602926113553679 2118 26-27 -0.13497451971083052 2118 28-29 0.26734646785592986 2118 30-31 0.21952085825727607 2118 32-33 0.21512740907421346 2118 34-35 0.2920353309528565 2118 36-37 0.22140494736186156 2118 38-39 -0.13100551645161573 2118 40-41 0.2886768156158155 2118 42-43 -0.05262438987978868 2118 44-45 0.0963927161563447 2118 46-47 0.0937130992434092 2118 48-49 0.1758293080405764 2118 50-51 0.1693162160517545 2118 52-53 0.2586529516719125 2118 54-55 0.39135539105634365 2118 56-57 0.2711804501681172 2118 58-59 0.09499420156747362 2118 60-61 0.29472396491307506 2118 62-63 0.4321315692186687 2118 64-65 0.2207018303385695 2118 66-67 0.3372087061125555 2118 68-69 -0.11599656066124453 2118 70-71 0.22490649533791185 2118 72-73 0.09314014236660029 2118 74-75 0.0852963446745818 2118 76 -0.1656319134014197 2119 1 0.0583266393886106 2119 2 0.17245869908566647 2119 3 0.09037857771367186 2119 4 0.11491852892054055 2119 5 -0.048466563073063185 2119 6 0.09959010907504506 2119 7 0.1574277668007369 2119 8 0.00559573925220036 2119 9 -0.006769246170044596 2119 10-11 0.01625246218063836 2119 12-13 -0.01992091897801629 2119 14-15 -0.4608571154025256 2119 16-17 -0.04693580472152803 2119 18-19 0.18544573152205857 2119 20-21 -0.007743372092804179 2119 22-23 -0.036102478487357814 2119 24-25 0.05685312477189797 2119 26-27 0.22449517725885926 2119 28-29 0.061711998158962444 2119 30-31 -0.09335792962151146 2119 32-33 0.09325240907421772 2119 34-35 0.09284025519527717 2119 36-37 0.0391606291800457 2119 38-39 0.1468827411241378 2119 40-41 0.19464272470672483 2119 42-43 0.06470515557475665 2119 44-45 -0.049819405055771426 2119 46-47 0.14461272045553386 2119 48-49 -0.13491879802003126 2119 50-51 0.09980864029417091 2119 52-53 0.011493860762818997 2119 54-55 0.12629857287452495 2119 56-57 0.07596264713781409 2119 58-59 0.1894070803553518 2119 60-61 0.1718072982464065 2119 62-63 0.18677172073381598 2119 64-65 -0.059222412085674137 2119 66-67 0.19431097883983028 2119 68-69 0.03248828782360391 2119 70-71 -0.03186433799542243 2119 72-73 0.2684242332756881 2119 74-75 0.5012054355836781 2119 76 -0.4695144891589962 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 0.0 23 10.0 24 26.0 25 66.0 26 119.0 27 211.0 28 416.0 29 652.0 30 1029.0 31 1641.0 32 2516.0 33 4143.0 34 6568.0 35 11116.0 36 16400.0 37 79158.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.86175768908375 14.662454058933521 10.682506931459153 33.79328132052357 2 22.911696434328455 18.137049455155072 32.58430588690438 26.3669482236121 3 19.952930556451093 17.469695015797278 31.22541749951641 31.351956928235218 4 15.59417112644271 21.546360177961184 31.040847249983884 31.818621445612226 5 16.03020826616803 28.512476626474946 37.55480688632407 17.90250822103295 6 29.998710426204138 35.68895480043845 16.67338319685344 17.638951576503967 7 29.208846476239604 24.84363917725192 20.163292281900834 25.784222064607647 8 25.197465987491135 35.41008446708363 15.772293506995938 23.6201560384293 9 23.181700947836738 16.749951640982655 16.326004255593528 43.74234315558708 10-11 26.510413308401574 25.297004964859116 23.126894061512672 25.065687665226644 12-13 25.769311367593012 18.86525565800503 25.413066606486556 29.952366367915406 14-15 22.850038687213875 18.352247082339286 20.426042942807403 38.37167128763944 16-17 27.900332065252435 24.387049455155072 19.49271390805339 28.219904571539107 18-19 29.544941646785738 23.263508285511637 20.054484492875105 27.137065574827517 20-21 24.95405893352247 21.385566445289832 20.34584757237733 33.31452704881037 22-23 23.75757624605068 25.396140950415884 20.157650396543943 30.68863240698949 24-25 27.98213940292733 24.765861757689084 21.196966277645238 26.055032561738344 26-27 28.98558901283126 26.055032561738344 20.461103230382356 24.498275195048038 28-29 25.346572957637502 25.726594235605134 20.994664388419626 27.93216841833774 30-31 23.775307885743764 23.026549100522278 28.081275388484105 25.116867625249856 32-33 26.780014830098654 20.725465858533752 24.794877168095944 27.699642143271646 34-35 27.734299439035397 20.11291830550003 21.909455799858147 30.24332645560642 36-37 30.41137404087949 22.919756270552583 21.67652653298085 24.99234315558708 38-39 23.438406731575213 23.2735830807918 26.596250564188534 26.69175962344445 40-41 22.74002192275453 19.557192597846413 27.781449480946545 29.921335998452513 42-43 26.45359146302147 19.167902508221033 27.32606873428332 27.052437294474174 44-45 21.832484363917725 23.436391772519183 30.049084402604937 24.68203946095815 46-47 24.474901669998065 20.461506222193567 27.986169321039394 27.077422786768974 48-49 24.229882648784574 19.450399767876718 25.29861693210394 31.021100651234768 50-51 21.767199690502288 20.751257334450965 28.36699658262944 29.114546392417306 52-53 18.602907988909664 20.83790057386034 34.19345218905152 26.36573924817848 54-55 19.01395963634019 21.479463537300923 30.48270359146302 29.02387323489587 56-57 22.11619059900703 21.99730801470114 26.014733380617706 29.871768005674127 58-59 19.530998130117997 22.072264491585532 30.705155071248953 27.69158230704752 60-61 22.5333779312735 25.1254317067175 26.020077132943502 26.321113229065496 62-63 19.086978073127188 32.19957846886674 22.231938841716268 26.481504616289804 64-65 18.557369914243342 33.732026565220195 21.046247340254045 26.664356180282418 66-67 18.831404345863692 33.25931717067509 20.416774131149655 27.49250435231156 68-69 18.514652782255464 33.88838738796828 20.283786833451543 27.313172996324713 70-71 18.834225288542136 32.952237410535815 20.96645496163518 27.247082339286866 72-73 19.18644013153653 30.44562834483203 21.882858340318524 28.485073183312913 74-75 19.569282352182604 29.47764201431427 22.297939905861114 28.655135727642012 76 19.717583338706557 29.364401315365274 21.9316203494745 28.986394996453672 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 1.5 16 3.0 17 4.0 18 6.0 19 11.0 20 19.0 21 24.0 22 33.0 23 50.5 24 65.5 25 72.0 26 116.5 27 204.5 28 300.5 29 353.0 30 444.5 31 596.5 32 849.0 33 1041.0 34 1222.0 35 1572.5 36 1907.5 37 2073.0 38 2396.5 39 3025.5 40 3768.5 41 6003.5 42 7801.0 43 8796.0 44 9489.5 45 8324.5 46 7461.0 47 6904.5 48 6006.0 49 5290.0 50 4916.0 51 4552.0 52 3864.0 53 3266.5 54 2993.0 55 2940.0 56 2713.0 57 2531.5 58 2524.0 59 2524.0 60 2653.5 61 2887.5 62 2992.0 63 3114.0 64 3312.5 65 3351.0 66 3248.0 67 3183.0 68 3144.5 69 2942.0 70 2604.0 71 2430.0 72 2269.0 73 1833.0 74 1315.0 75 1072.0 76 844.5 77 468.0 78 206.5 79 94.0 80 56.5 81 13.5 82 4.5 83 1.0 84 1.0 85 1.0 86 0.5 87 0.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 4.029918112063963E-4 62-63 4.029918112063963E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 124072.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.35360113482494 #Duplication Level Percentage of deduplicated Percentage of total 1 64.34333866806023 31.75575472306403 2 20.656824639905935 20.389773679798825 3 7.102263448411013 10.515668321619705 4 2.9052487180324658 5.735379457089432 5 1.4861024920795636 3.6672254819782064 6 0.9602508410360258 2.843510219872332 7 0.6401672273573504 2.211619059900703 8 0.42296763236110657 1.669998065639306 9 0.34621288826468954 1.5378167515636083 >10 1.1055949309207302 7.809175317557547 >50 0.013064637293007154 0.4440969759494487 >100 0.011431557631381258 1.1710942033657876 >500 0.0 0.0 >1k 0.004899238984877682 4.4844928751047775 >5k 0.0016330796616258942 5.764394867496293 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 7152 5.764394867496293 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 2039 1.643400606099684 No Hit TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG 1878 1.5136372428912244 No Hit CACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTTCTG 1647 1.3274550261138693 RNA PCR Primer, Index 18 (95% over 24bp) TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 471 0.3796182861564253 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT 245 0.1974659874911342 No Hit ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT 222 0.17892836417563995 RNA PCR Primer, Index 30 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT 180 0.14507705203430266 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 8.059836224127926E-4 0.0 2 0.0 0.0 0.0 8.059836224127926E-4 0.0 3 0.0 0.0 0.0 0.0032239344896511702 0.0 4 0.0 0.0 0.0 0.008059836224127925 0.0 5 0.0 0.0 0.0 0.012089754336191888 0.0 6 0.0 0.0 0.0 0.012089754336191888 0.0 7 0.0 0.0 0.0 0.012895737958604681 0.0 8 0.0 0.0 0.0 0.012895737958604681 0.0 9 0.0 0.0 0.0 0.017731639693081436 0.0 10 0.0 0.0 0.0 0.020955574182732608 0.0 11 0.0 0.0 0.0 0.0217615578051454 0.0 12 0.0 0.0 0.0 0.0217615578051454 0.0 13 0.0 0.0 0.0 0.024179508672383776 0.0 14 0.0 0.0 0.0 0.02498549229479657 0.0 15 0.0 0.0 0.0 0.027403443162034948 0.0 16 0.0 0.0 0.0 0.02820942678444774 0.0 17 0.0 0.0 0.0 0.029015410406860533 0.0 18 0.0 0.0 0.0 0.03143336127409891 0.0 19 0.0 0.0 0.0 0.039493197498226836 0.0 20 0.0 0.0 0.0 0.04110516474305242 0.0 21 0.0 0.0 0.0 0.04271713198787801 0.0 22 0.0 0.0 0.0 0.04432909923270359 0.0 23 0.0 0.0 0.0 0.04594106647752918 0.0 24 0.0 0.0 0.0 0.04755303372235476 0.0 25 0.0 0.0 0.0 0.054806886324069895 0.0 26 0.0 0.0 0.0 0.07737442775162809 0.0 27 0.0 0.0 0.0 0.17812238055322716 0.0 28 0.0 0.0 0.0 0.30788574376168676 0.0 29 0.0 0.0 0.0 0.42878328712360564 0.0 30 0.0 0.0 0.0 0.5738603391579084 0.0 31 0.0 0.0 0.0 0.7165194403249726 0.0 32 0.0 0.0 0.0 0.9067315752143916 0.0 33 0.0 0.0 0.0 1.1162873170417178 0.0 34 0.0 0.0 0.0 1.407247404732736 8.059836224127926E-4 35 0.0 0.0 0.0 1.826358888387388 8.059836224127926E-4 36 0.0 0.0 0.0 2.423592752595267 8.059836224127926E-4 37 0.0 0.0 0.0 3.245696047456316 8.059836224127926E-4 38 0.0 0.0 0.0 4.2056225417499515 8.059836224127926E-4 39 0.0 0.0 0.0 5.113160100586756 8.059836224127926E-4 40 0.0 0.0 0.0 6.28022438584048 8.059836224127926E-4 41 0.0 0.0 0.0 7.531916951447546 8.059836224127926E-4 42 0.0 0.0 0.0 8.858565993939003 8.059836224127926E-4 43 0.0 0.0 0.0 10.221484299439036 8.059836224127926E-4 44 0.0 0.0 0.0 11.508640144432265 8.059836224127926E-4 45 0.0 0.0 0.0 12.646689019279128 8.059836224127926E-4 46 0.0 0.0 0.0 13.733960925913985 8.059836224127926E-4 47 0.0 0.0 0.0 14.737410535817911 8.059836224127926E-4 48 0.0 0.0 0.0 15.69491907924431 8.059836224127926E-4 49 0.0 0.0 0.0 16.574247211296665 8.059836224127926E-4 50 0.0 0.0 0.0 17.425365916564576 0.0016119672448255851 51 0.0 0.0 0.0 18.36191888580824 0.0016119672448255851 52 0.0 0.0 0.0 19.420175382036238 0.0016119672448255851 53 0.0 0.0 0.0 20.518731059384873 0.0016119672448255851 54 0.0 0.0 0.0 21.643078212650718 0.0016119672448255851 55 0.0 0.0 0.0 22.62799019923915 0.0016119672448255851 56 0.0 0.0 0.0 23.513766200270812 0.0016119672448255851 57 0.0 0.0 0.0 24.29073441227674 0.0016119672448255851 58 0.0 0.0 0.0 25.070926558772324 0.0016119672448255851 59 0.0 0.0 0.0 25.84547681991102 0.0016119672448255851 60 0.0 0.0 0.0 26.587787736153203 0.0016119672448255851 61 0.0 0.0 0.0 27.297053323876458 0.0016119672448255851 62 0.0 0.0 0.0 28.035334322006577 0.0016119672448255851 63 0.0 0.0 0.0 28.854213682377974 0.0016119672448255851 64 0.0 0.0 0.0 29.651331484944226 0.0016119672448255851 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGTTC 15 0.0022202157 70.00001 27 CACTGAG 15 0.0022202157 70.00001 7 GAAAGCG 15 0.0022202157 70.00001 24 ATCCCAA 15 0.0022202157 70.00001 59 CGACTGG 15 0.0022202157 70.00001 35 AAAGCGT 15 0.0022202157 70.00001 25 GCTTATA 30 8.336288E-6 58.333336 3 TCTTTAT 60 1.8189894E-12 58.333336 1 GAAGCGC 20 0.0069375 52.5 21 AAGCGTT 20 0.0069375 52.5 26 ATAGACC 20 0.0069375 52.5 14 CACTACC 20 0.0069375 52.5 46 ACCAATT 20 0.0069375 52.5 15 TCAAGCT 20 0.0069375 52.5 32 ATCATGT 20 0.0069375 52.5 15 CTTTATA 70 7.2759576E-12 50.0 2 ATCGTAT 30 5.859032E-4 46.666668 39 GATATCG 30 5.859032E-4 46.666668 36 TATCCTG 30 5.859032E-4 46.666668 38 TTTATAC 115 0.0 42.6087 3 >>END_MODULE